@article{ShityakovFoersterRethwilmetal.2014,
  author    = {Shityakov, Sergey and F{\"o}rster, Carola and Rethwilm, Axel and Dandekar, Thomas},
  title     = {Evaluation and Prediction of the HIV-1 Central Polypurine Tract Influence on Foamy Viral Vectors to Transduce Dividing and Growth-Arrested Cells},
  doi       = {10.1155/2014/487969},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-112763},
  year      = {2014},
  abstract  = {Retroviral vectors are potent tools for gene delivery and various biomedical applications. To accomplish a gene transfer task successfully, retroviral vectors must effectively transduce diverse cell cultures at different phases of a cell cycle. However, very promising retroviral vectors based on the foamy viral (FV) backbone lack the capacity to efficiently transduce quiescent cells. It is hypothesized that this phenomenon might be explained as the inability of foamy viruses to form a pre-integration complex (PIC) with nuclear import activity in growth-arrested cells, which is the characteristic for lentiviruses (HIV-1). In this process, the HIV-1 central polypurine tract (cPPT) serves as a primer for plus-strand synthesis to produce a "flap" element and is believed to be crucial for the subsequent double-stranded cDNA formation of all retroviral RNA genomes. In this study, the effects of the lentiviral cPPT element on the FV transduction potential in dividing and growth-arrested (G1/S phase) adenocarcinomic human alveolar basal epithelial (A549) cells are investigated by experimental and theoretical methods. The results indicated that the HIV-1 cPPT element in a foamy viral vector background will lead to a significant reduction of the FV transduction and viral titre in growth-arrested cells due to the absence of PICs with nuclear import activity.},
  subject      = {Evaluation},
  language  = {en}
}
@inproceedings{MaurerBannertRethwilmetal.1988,
  author    = {Maurer, B. and Bannert, H. and Rethwilm, Axel and Darai, B. and Fl{\"u}gel, R. M.},
  title     = {Characterization of the env gene and of two novel coding regions of the human spumaretrovirus},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86334},
  year      = {1988},
  abstract  = {Recombinant clones harboring retroviral DNA were established. The nucleotide sequence of the central and 3' region of the genome of the human spumaretrovirus was determined. The 5' end of the deduced protein sequence was homologaus to the endonuclease domain of retroviral reverse transcriptases. A small intergenic region is followed by a lang open reading frame of 985 aminoacid residues that according to its genomic location and structural features is a typical retroviral env gene. Surprisingly, the postenv region contains two open reading frames that encodes two novel retroviral genes, termed bel-l and bel-2. The 3' LTR is 963 nucleotides lang and contains the signal sequences characteristic for transcriptional regulation of retrovirus genomes.},
  subject      = {Spumaviren},
  language  = {en}
}