@article{SchiererOstaleckiZinseretal.2018, author = {Schierer, Stefan and Ostalecki, Christian and Zinser, Elisabeth and Lamprecht, Ricarda and Plosnita, Bianca and Stich, Lena and Doerrie, Jan and Lutz, Manfred B and Schuler, Gerold and Baur, Andreas S}, title = {Extracellular vesicles from mature dendritic cells (DC) differentiate monocytes into immature DC}, series = {Life Science Alliance}, volume = {1}, journal = {Life Science Alliance}, number = {6}, doi = {10.26508/lsa.201800093}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-228587}, pages = {e201800093, 1-17}, year = {2018}, abstract = {During inflammation, murine and human monocytes can develop into dendritic cells (DC), but this process is not entirely understood. Here, we demonstrate that extracellular vesicles (EV) secreted by mature human DC (maDC) differentiate peripheral monocytes into immature DC, expressing a unique marker pattern, including 6-sulfo LacNAc (slan), Zbtb46, CD64, and CD14. While EV from both maDC and immature DC differentiated monocytes similar to GM-CSF/IL-4 stimulation, only maDC-EV produced precursors, which upon maturation stimulus developed into T-cell-activating and IL-12p70-secreting maDC. Mechanistically, maDC-EV induced cell signaling through GM-CSF, which was abundant in EV as were IL-4 and other cytokines and chemokines. When injected into the mouse skin, murine maDC-EV attracted immune cells including monocytes that developed activation markers typical for inflammatory cells. Skin-injected EV also reached lymph nodes, causing a similar immune cell infiltration. We conclude that DC-derived EV likely serve to perpetuate an immune reaction and may contribute to chronic inflammation.}, language = {en} } @article{LuetkensErguenHuflageetal.2021, author = {Luetkens, Karsten Sebastian and Erg{\"u}n, S{\"u}leyman and Huflage, Henner and Kunz, Andreas Steven and Gietzen, Carsten Herbert and Conrads, Nora and Pennig, Lenhard and Goertz, Lukas and Bley, Thorsten Alexander and Gassenmaier, Tobias and Grunz, Jan-Peter}, title = {Dose reduction potential in cone-beam CT imaging of upper extremity joints with a twin robotic x-ray system}, series = {Scientific Reports}, volume = {11}, journal = {Scientific Reports}, number = {1}, doi = {10.1038/s41598-021-99748-1}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-270429}, year = {2021}, abstract = {Cone-beam computed tomography is a powerful tool for 3D imaging of the appendicular skeleton, facilitating detailed visualization of bone microarchitecture. This study evaluated various combinations of acquisition and reconstruction parameters for the cone-beam CT mode of a twin robotic x-ray system in cadaveric wrist and elbow scans, aiming to define the best possible trade-off between image quality and radiation dose. Images were acquired with different combinations of tube voltage and tube current-time product, resulting in five scan protocols with varying volume CT dose indices: full-dose (FD; 17.4 mGy), low-dose (LD; 4.5 mGy), ultra-low-dose (ULD; 1.15 mGy), modulated low-dose (mLD; 0.6 mGy) and modulated ultra-low-dose (mULD; 0.29 mGy). Each set of projection data was reconstructed with three convolution kernels (very sharp [Ur77], sharp [Br69], intermediate [Br62]). Five radiologists subjectively assessed the image quality of cortical bone, cancellous bone and soft tissue using seven-point scales. Irrespective of the reconstruction kernel, overall image quality of every FD, LD and ULD scan was deemed suitable for diagnostic use in contrast to mLD (very sharp/sharp/intermediate: 60/55/70\%) and mULD (0/3/5\%). Superior depiction of cortical and cancellous bone was achieved in FD\(_{Ur77}\) and LD\(_{Ur77}\) examinations (p < 0.001) with LD\(_{Ur77}\) scans also providing favorable bone visualization compared to FD\(_{Br69}\) and FD\(_{Br62}\) (p < 0.001). Fleiss' kappa was 0.618 (0.594-0.641; p < 0.001), indicating substantial interrater reliability. In this study, we demonstrate that considerable dose reduction can be realized while maintaining diagnostic image quality in upper extremity joint scans with the cone-beam CT mode of a twin robotic x-ray system. Application of sharper convolution kernels for image reconstruction facilitates superior display of bone microarchitecture.}, language = {en} } @article{LozovayaGataullinaTsintsadzeetal.2014, author = {Lozovaya, N. and Gataullina, S. and Tsintsadze, T. and Tsintsadze, V. and Pallesi-Pocachard, E. and Minlebaev, M. and Goriounova, N. A. and Buhler, E. and Watrin, F. and Shityakov, S. and Becker, A. J. and Bordey, A. and Milh, M. and Scavarda, D. and Bulteau, C. and Dorfmuller, G. and Delalande, O. and Represa, A. and Cardoso, C. and Dulac, O. and Ben-Ari, Y. and Burnashev, N.}, title = {Selective suppression of excessive GluN2C expression rescues early epilepsy in a tuberous sclerosis murine model}, series = {Nature Communications}, volume = {5}, journal = {Nature Communications}, number = {4563}, doi = {10.1038/ncomms5563}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-121276}, year = {2014}, abstract = {Tuberous sclerosis complex (TSC), caused by dominant mutations in either TSC1 or TSC2 tumour suppressor genes is characterized by the presence of brain malformations, the cortical tubers that are thought to contribute to the generation of pharmacoresistant epilepsy. Here we report that tuberless heterozygote \(Tsc1^{+/-}\) mice show functional upregulation of cortical GluN2C-containing N-methyl-D-aspartate receptors (NMDARs) in an mTOR-dependent manner and exhibit recurrent, unprovoked seizures during early postnatal life (