@article{MauelerEigenbrodtSchartl1987,
  author    = {Maueler, W. and Eigenbrodt, E. and Schartl, Manfred},
  title     = {Intermediary metabolism of normal and tumorous tissue of Xiphophorus (Teleostei: Poeciliidae)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61855},
  year      = {1987},
  abstract  = {No abstract available},
  subject      = {Physiologische Chemie},
  language  = {en}
}
@article{BarnekowSchartl1987,
  author    = {Barnekow, A. and Schartl, Manfred},
  title     = {Comparative studies on the src proto-oncogene and its gene product pp60\(^{c-src}\) in normal and neoplastic tissues of lower vertebrates},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61869},
  year      = {1987},
  abstract  = {No abstract available},
  subject      = {Physiologische Chemie},
  language  = {en}
}
@article{StopperJonesZimmermann1987,
  author    = {Stopper, Helga and Jones, H. and Zimmermann, U.},
  title     = {Large scale transfection of mouse L-cells by electropermeabilization},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63497},
  year      = {1987},
  abstract  = {Mouse L-cells were transfected by electropenneabilization using the selectable plasmid pSV2-neo which confers resistance to G-418 (Geneticin). 1be DNA concentration used was 1 l'gfml, the field strength was 10 kV fcm, the duration of the pulse was S ~s. Transfeetion yield was optimal at a temperature of 4°C when using a time in between consecutive pulses of 1 minute compared to shorter (of the order of seoonds) or Ionger (3 minutes) time intervals. A more detailed study of the relationship between the number of pulses applied (up to 10) and transfection yield showed it to be almost linear in this range at 4 o C. The yield of transfectants in response to 10 pulses was up to 1000 per 106 cells (using 3.3 pg DNA per cell). The inßuence of the growth phase of the cells on the transfection yield and I or the subpopulation of the mouse L--ceU line used was shown. Furthennore the clone yield depended on the DNA per ceU ratio within a very small range.},
  subject      = {Toxikologie},
  language  = {en}
}
@article{HollerFischerWeberetal.1987,
  author    = {Holler, E. and Fischer, H. and Weber, C. and Stopper, Helga and Steger, H. and Simek, H.},
  title     = {A DNA polymerase with unusual properties from the slime mold Physarum polycephalum},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63501},
  year      = {1987},
  abstract  = {Two forms of a DNA polymerase have been purified from microplasmodia of Physarum polycephalum by poly(ethyleneimine) precipitation and chromatography on DEAE-Sephacel, phosphocellulose, heparin Sepharose, hydroxyapatite, DNA-agarose, blue-Sepharose. They were separated from DNA polymerase cx on phosphocellulose and from each other on heparin-Sepharose. Form HS1 enzymewas 30-40\% pure and form HS2 enzyme 60\% with regard toprotein contents of the preparations. Form HS2 enzymewas generated from form HS1 enzyme on prolonged standing of enzyme preparations. The DNA polymerases were obtained as complexes of a 60-kDa protein associated with either a 135-kDa (HS1) or a 110-kDa (HS2) DNA-polymerizing polypeptidein a 1:1 molar stoichiometry. The biochemical function of the 60-kDa protein remained unknown. The complexes tended to dissociate during gradient centrifugation and during partition chromatography as weil as during polyacrylamide gradient gel electrophoresis under nondenaturing conditions at high dilutions of samples. Both forms existed in plasmodia extracts, their proportions depending on several factors including those which promoted proteolysis. The DNA polymerases resembled eucaryotic DNA polymerase ß by several criteria and were functionally indistinguishable from each other. It is suggested that lower eucaryotes contain repair DNA polymerases, which are similar to those of eubacteria on a molecular mass basis.},
  subject      = {Toxikologie},
  language  = {en}
}
@article{ZimmermannStopper1987,
  author    = {Zimmermann, U. and Stopper, Helga},
  title     = {Elektrofusion und Elektropermeabilisierung von Zellen : Eine neuartige Methode der Biotechnologie zur gezieltenVer{\"a}nderung der genetischen Eigenschaften von Zellen},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63514},
  year      = {1987},
  abstract  = {No abstract available},
  subject      = {Toxikologie},
  language  = {en}
}
@article{SchneiderSchauliesSchimplWecker1987,
  author    = {Schneider-Schaulies, J{\"u}rgen and Schimpl, A. and Wecker, E.},
  title     = {Kinetics of cellular oncogene expression in mouse lymphocytes II. Regulation of c-fos and c-myc gene expression},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-54823},
  year      = {1987},
  abstract  = {Newly isolated lymphocytes from mousespleensexpress the c-fos oncogene even in the absence of mitogen with maximal mRNA levels 60 min post preparation of single cell suspension, whereas c-myc mRNA Ievels increase only after mitogenic Stimulation with maximal mRNA Ievels 6 h post Stimulation. The half-lives of c-fos mRNA are generally very short; they increase from 14 min (after 30 min of culture) to 70 min (after 2 h of culture). The half-lives of c-myc mRNA decrease from 50 min (at 2 and 6 h post stimulation with concanavalin A) to 12 min (at 48 h post stimulation). The c-fos gene transcription is already tumed on in time-0 lymphocytes 10 min after disruption of the organ structure of the spleens and is down-regulated after 2 h and later. In nuclear run-on experiments with nonstimulated lymphocytes there is already significant transcription of the first exon of c-myc, but almost no elongation of the transcript to exon 2 and 3. In concanavalin A-treated lymphocytes elongation is stimulated about 5-fold within 6 h and returns to background levels at 48 h post Stimulation. · The nuclear run-on analyses of nonactivated lymphocytes showed a signal for RNA complementary to c-myc mRNA detected with a probe specific for the exon 1/intron 1 boundary of c-myc, which disappeared with increasing time of concanavalin A Stimulation. This anti-sense transcription may play a role in regulating the elongation of cmyc transcripts.},
  subject      = {Lymphozyt},
  language  = {en}
}
@article{EngelsPeyerimhoffDavidson1987,
  author    = {Engels, Bernd and Peyerimhoff, S.D. and Davidson, E.R.},
  title     = {Calculation of hyperfine coupling constants : An ab initio MRD-CI study for nitrogen to analyse the effects of the basis sets and CI parameter},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-58784},
  year      = {1987},
  abstract  = {The hyperfine coupling constant for the nitrogen atom is evaluated by large-scale MRD-CI calculations. A detailed analysis of the charge density at the nucleus and the spin polarization in the ls and 2s shell as a function of various technical parameters is undertaken. Various (s, p) AO basis sets and the inftuence of correlation orbitals is investigated as weil as selection threshold and other properlies in CI calculations. The best value, obtained for the isotropic hyperfine coupling constant in an s, p, d basis, based on theoretical judgment of' best' quantities, is 9·9 MHz compared to 10·4509 MHz.},
  subject      = {Organische Chemie},
  language  = {en}
}
@article{HackerUlmerFasskeetal.1987,
  author    = {Hacker, J{\"o}rg and Ulmer, E. and Fasske, E. and Schmidt, G.},
  title     = {Isolation and characterization of coliphage Omega18A specific for Escherichia coli O18ac strains},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-73001},
  year      = {1987},
  abstract  = {The bactedophage Q18A, specific for Escherichia coli 018ac srrains, was isolated frorn sewage. The results of host range and conjugation experiments showed that the sensitivity of bacteria to the phage is associated with rhe presence of 018ac antigens. With sorne of rhe 018 strains rhe phage Q18A produces clear Iysis on bacterial lawns only when applied at a high multiplicity and moreover the phage does not multiply. With rhe help of the phage Ql8A, E. coli 0 18ac strains could be divided inro rwo serologically clistinct subgroups called 018A and 018A1• E. coli strains belanging to the sugroup 0 ISAare sensitive to phage Q t8A wheteas bacteria of subgroup A1 are resistanr.},
  subject      = {Escherichia coli},
  language  = {en}
}
@article{FeuersteinLeaderSirenetal.1987,
  author    = {Feuerstein, G. and Leader, P. and Sir{\´e}n, Anna-Leena and Braquet, P.},
  title     = {Protective effect of PAF-acether antagonist, BN 52021, in trichothecen toxicosis},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63244},
  year      = {1987},
  abstract  = {Trichothecenes are mycotoxins which produce Iethai toxicosis in humans and animals, yet no adequate therapeutic regimen has been developed. This study provides evidence that the selective platelet activating factor (PAF) antagonist, BN 52021 (5-15 mg/kg i.v.) can prolong the survival of conscious rats exposed to a highly Iethai T -2 toxicosis. These data also suggest that P AF is an important mediator of this unique toxicosis.},
  subject      = {Neurobiologie},
  language  = {en}
}
@article{LabrooCohenLozovskyetal.1987,
  author    = {Labroo, V. M. and Cohen, L. A. and Lozovsky, D. and Sir{\´e}n, Anna-Leena and Feuerstein, G.},
  title     = {Dissociation of the cardiovascular and prolactin-releasing activities of TRH by histidine replacement},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63253},
  year      = {1987},
  abstract  = {No abstract available},
  subject      = {Neurobiologie},
  language  = {en}
}