@article{RaduSchoenwetterBraunetal.2017,
  author    = {Radu, Laura and Schoenwetter, Elisabeth and Braun, Cathy and Marcoux, Julien and Koelmel, Wolfgang and Schmitt, Dominik R. and Kuper, Jochen and Cianf{\´e}rani, Sarah and Egly, Jean M. and Poterszman, Arnaud and Kisker, Caroline},
  title     = {The intricate network between the p34 and p44 subunits is central to the activity of the transcription/DNA repair factor TFIIH},
  series = {Nucleic Acids Research},
  volume    = {45},
  journal   = {Nucleic Acids Research},
  number    = {18},
  doi       = {10.1093/nar/gkx743},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-170173},
  pages     = {10872-10883},
  year      = {2017},
  abstract  = {The general transcription factor IIH (TFIIH) is a multi-protein complex and its 10 subunits are engaged in an intricate protein-protein interaction network critical for the regulation of its transcription and DNA repair activities that are so far little understood on a molecular level. In this study, we focused on the p44 and the p34 subunits, which are central for the structural integrity of core-TFIIH. We solved crystal structures of a complex formed by the p34 N-terminal vWA and p44 C-terminal zinc binding domains from Chaetomium thermophilum and from Homo sapiens. Intriguingly, our functional analyses clearly revealed the presence of a second interface located in the C-terminal zinc binding region of p34, which can rescue a disrupted interaction between the p34 vWA and the p44 RING domain. In addition, we demonstrate that the C-terminal zinc binding domain of p34 assumes a central role with respect to the stability and function of TFIIH. Our data reveal a redundant interaction network within core-TFIIH, which may serve to minimize the susceptibility to mutational impairment. This provides first insights why so far no mutations in the p34 or p44 TFIIH-core subunits have been identified that would lead to the hallmark nucleotide excision repair syndromes xeroderma pigmentosum or trichothiodystrophy.},
  language  = {en}
}