@phdthesis{Geier2021,
  author    = {Geier, Bettina},
  title     = {Kernspintomografische Natriumbildgebung in Haut und Muskel},
  doi       = {10.25972/OPUS-24942},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-249429},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2021},
  abstract  = {Die vorliegende Arbeit untersucht den Natriumgehalt verschiedener Kompartimente des K{\"o}rpers mittels Magnetresonanztomographie (= MRT). Die Korrelation zwischen erh{\"o}htem Salzkonsum und arterieller Hypertonie ist bereits umfangreich analysiert worden. F{\"u}r das Verst{\"a}ndnis der pathophysiologischen Zust{\"a}nde und deren Regulation, ist eine Quantifizierung von Natriumkonzentrationen in verschiedenen Gewebearten bedeutsam. Die exakte Messung von Natriumkonzentrationen im menschlichen Gewebe ist derzeit experimentell. Im Rahmen der hier vorgelegten Arbeit wurden die Natriumkonzentrationen von Haut und Skelettmuskel mittels 23Na Magnetresonanztomographie (= 23 Na MRT) im menschlichen K{\"o}rper quantifiziert. Natriummessungen wurden bei Patienten mit prim{\"a}rem Hyperaldosteronismus (= PHA), bei Patienten mit essentieller Hypertonie (= EH), sowie einer gesunden Kontrollgruppe vorgenommen. Die Ergebnisse zeigten, dass Haut und Skelettmuskel Speicherorgane f{\"u}r Natrium im menschlichen K{\"o}rper darstellen. Durch gezielte Therapie waren die Natriumkonzentrationen in beiden Speicherorganen modulierbar},
  subject      = {Natrium-23},
  language  = {de}
}
@article{UeberschaarGoebelerKneitz2020,
  author    = {Ueberschaar, Simon and Goebeler, Matthias and Kneitz, Hermann},
  title     = {CD10-Positive Cutaneous PEComa: An Extremely Rare Skin Tumour},
  series = {Case Reports in Dermatology},
  volume    = {12},
  journal   = {Case Reports in Dermatology},
  doi       = {10.1159/000510718},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-236151},
  pages     = {192-198},
  year      = {2020},
  abstract  = {We here present the case of a 67-year-old woman with a history of a slowly progressive, polypous nodule on her left wrist. The lesion was excised, and the histological analysis revealed a clear cell tumour that was relatively sharply demarked from the surrounding tissue extending into the subcutaneous tissue. The tumour showed a characteristic trabecular pattern in which the tumour cells were arranged around numerous vessels. The neoplastic cells had a predominantly epithelioid shape, granular eosinophilic to clear cytoplasm and prominent centrally located nucleoli. The histological differential diagnosis included a metastatic clear-cell renal cell carcinoma and a primary cutaneous perivascular epithelioid cell tumour (PEComa). Immunohistochemically, the tumour cells revealed homogenous expression of HMB-45, MiTF and CD10, whereas MART-1 and S100 were negative. Antibodies against actin marked the trabecularly arranged vessels, and the neoplastic cells yielded a patchy positivity against actin and desmin. Additional immunohistochemical stains against pan-cytokeratin, CAIX, PAX-8 and EMA were negative. Based on the morphologic and immunophenotypic findings, the histological diagnosis of a CD10-positive cutaneous PEComa was made.},
  language  = {en}
}
@article{AbimannanSumathiKrishnarajasekharetal.2019,
  author    = {Abimannan, Nagarajan and Sumathi, G. and Krishnarajasekhar, O. R. and Sinha, Bhanu and Krishnan, Padma},
  title     = {Clonal Clusters and Virulence Factors of Methicillin-Resistant \(Staphylococcus\) \(Aureus\): Evidence for Community-Acquired Methicillin-Resistant \(Staphylococcus\) \(Aureus\) Infiltration into Hospital Settings in Chennai, South India},
  series = {Indian Journal of Medical Microbiology},
  volume    = {37},
  journal   = {Indian Journal of Medical Microbiology},
  number    = {3},
  doi       = {10.4103/ijmm.IJMM_18_271},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-226963},
  pages     = {326-336},
  year      = {2019},
  abstract  = {Background and Objective: Staphylococcus aureus is one of the major pathogens of nosocomial infections as wells as community-acquired (CA) infections worldwide. So far, large-scale comprehensive molecular and epidemiological characterisation of S. aureus from very diverse settings has not been carried out in India. The objective of this study is to evaluate the molecular, epidemiological and virulence characteristics of S. aureus in both community and hospital settings in Chennai, southern India. Methods: S. aureus isolates were obtained from four different groups (a) healthy individuals from closed community settings, (b) inpatients from hospitals, (c) outpatients from hospitals, representing isolates of hospital-community interface and (d) HIV-infected patients to define isolates associated with the immunocompromised. Antibiotic susceptibility testing, multiplex polymerase chain reactions for detection of virulence and resistance determinants, molecular typing including Staphylococcal cassette chromosome mec (SCCmec) and agr typing, were carried out. Sequencing-based typing was done using spa and multilocus sequence typing (MLST) methods. Clonal complexes (CC) of hospital and CA methicillin-resistant S. aureus (MRSA) were identified and compared for virulence and resistance. Results and Conclusion: A total of 769 isolates of S. aureus isolates were studied. The prevalence of MRSA was found to be 7.17\%, 81.67\%, 58.33\% and 22.85\% for groups a, b, c and d, respectively. Of the four SCCmec types (I, III, IV and V) detected, SCCmec V was found to be predominant. Panton-Valentine leucocidin toxin genes were detected among MRSA isolates harbouring SCCmec IV and V. A total of 78 spa types were detected, t657 being the most prevalent. 13 MLST types belonging to 9 CC were detected. CC1 (ST-772, ST-1) and CC8 (ST238, ST368 and ST1208) were found to be predominant among MRSA. CA-MRSA isolates with SCCmec IV and V were isolated from all study groups including hospitalised patients and were found to be similar by molecular tools. This shows that CA MRSA has probably infiltrated into the hospital settings.},
  language  = {en}
}
@phdthesis{Rossi2017,
  author    = {Rossi, Angela Francesca},
  title     = {Development of functionalized electrospun fibers as biomimetic artificial basement membranes},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-137618},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2017},
  abstract  = {The basement membrane separates the epithelium from the stroma of any given barrier tissue and is essential in regulating cellular behavior, as mechanical barrier and as structural support. It further plays an important role for new tissue formation, homeostasis, and pathological processes, such as diabetes or cancer. Breakdown of the basement membrane is believed to be essential for tumor invasion and metastasization. Since the basement membrane is crucial for many body functions, the development of artificial basement membranes is indispensable for the ultimate formation of engineered functional tissue, however, challenging due to their complex structure. Electrospinning enables the production of fibers in the nano- or microscale range with morphological similarities to the randomly orientated collagen and elastic fibers in the basement membrane. However, electrospun fibers often lack the functional similarity to guide cells and maintain tissue-specific functions. Hence, their possible applications as matrix structure for tissue engineering are limited. Herein, the potential of polyester meshes, modified with six armed star-shaped pre-polymers and cell-adhesion-mediating peptides, was evaluated to act as functional isotropic and bipolar artificial basement membranes. Thereby, the meshes were shown to be biocompatible and stable including under dynamic conditions, and the degradation profile to correlate with the rate of new tissue formation. The different peptide sequences did not influence the morphology and integrity of the fibers. The modified membranes exhibited protein-repellent properties over 12 months, indicating the long-term stability of the cross-linked star-polymer surfaces. Cell culture experiments with primary fibroblasts and a human keratinocyte cell line (HaCaT) revealed that cell adhesion and growth strongly depends on the peptide sequences and their combinations employed. HaCaT cells grew to confluence on membranes modified with a combination of laminin/collagen type IV derived binding sequences and with a combination of fibronectin/laminin/collagen type IV derived peptide sequences. Fibroblasts strongly adhered to the fibronectin derived binding sequence and to membranes containing a combination of fibronectin/laminin/collagen type IV derived peptide sequences. The adhesion and growth of fibroblasts and HaCaT cells were significantly reduced on membranes modified with laminin, as well as collagen IV derived peptide sequences. HaCaT cells and fibroblasts barely adhered onto meshes without peptide sequences. Co-culture experiments at the air-liquid interface with fibroblasts and HaCaT cells confirmed the possibility of creating biocompatible, biofunctional and biomimetic isotropic and bipolar basement membranes, based on the functionalized fibers. HaCaT cells grew in several layers, differentiating towards the surface and expressing cytokeratin 10 in the suprabasal and cytokeratin 14 in the basal layers. Migration of fibroblasts into the electrospun membrane was shown by vimentin staining. Moreover, specific staining against laminin type V, collagen type I, III, IV and fibronectin illustrated that cells started to remodel the electrospun membrane and produced new extracellular matrix proteins following the adhesion to the synthetic surface structures. The culturing of primary human skin keratinocytes proved to be difficult on electrospun fibers. Cells attached to the membrane, but failed to form a multilayered, well-stratified, and keratinized epidermal layer. Changing the fiber composition and fixation methods did not promote tissue development. Further investigations of the membrane demonstrated the tremendous influence of the pore size of the membrane on epithelial formation. Furthermore, primary keratinocytes reacted more sensitive to pH changes in the medium than HaCaT cells did. Since primary keratinocytes did not adequately develop on the functionalized meshes, polycarbonate membranes were used instead of electrospun meshes to establish oral mucosa models. The tissue-engineered models represented important features of native human oral mucosa. They consisted of a multilayered epithelium with stratum basale, stratum spinosum, stratum granulosum, and stratum corneum. The models formed a physical barrier and the expression of characteristic cell markers was comparable with that in native human oral mucosa. The results from the ET-50 assay and the irritation study reflected the reproducibility of the tissue equivalents. Altogether, electrospinning enables the production of fibers with structural similarity to the basement membrane. Incorporating extracellular matrix components to mimic the functional composition offers a safe and promising way to modify the fibers so that they can be used for different tissue engineering applications. The resultant biomimetic membranes that can be functionalized with binding sequences derived from widely varying proteins can be used as a toolbox to study the influence of isotropic and bipolar basement membranes on tissue formation and matrix remodeling systematically, with regards to the biochemical composition and the influence and importance of mono- and co-culture. The oral mucosa models may be useful for toxicity and permeation studies, to monitor the irritation potential of oral health care products and biomaterials or as a disease model.},
  subject      = {Tissue Engineering},
  language  = {en}
}
@article{PlauthGeikowskiCichonetal.2016,
  author    = {Plauth, Annabell and Geikowski, Anne and Cichon, Susanne and Wowro, Sylvia J. and Liedgens, Linda and Rousseau, Morten and Weidner, Christopher and Fuhr, Luise and Kliem, Magdalena and Jenkins, Gail and Lotito, Silvina and Wainwright, Linda J. and Sauer, Sascha},
  title     = {Hormetic shifting of redox environment by pro-oxidative resveratrol protects cells against stress},
  series = {Free Radical Biology and Medicine},
  volume    = {99},
  journal   = {Free Radical Biology and Medicine},
  doi       = {10.1016/j.freeradbiomed.2016.08.006},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-187186},
  pages     = {608-622},
  year      = {2016},
  abstract  = {Resveratrol has gained tremendous interest owing to multiple reported health-beneficial effects. However, the underlying key mechanism of action of this natural product remained largely controversial. Here, we demonstrate that under physiologically relevant conditions major biological effects of resveratrol can be attributed to its generation of oxidation products such as reactive oxygen species (ROS). At low nontoxic concentrations (in general < 50 mu M), treatment with resveratrol increased viability in a set of representative cell models, whereas application of quenchers of ROS completely truncated these beneficial effects. Notably, resveratrol treatment led to mild, Nrf2-specific gene expression reprogramming. For example, in primary epidermal keratinocytes derived from human skin this coordinated process resulted in a 1.3-fold increase of endogenously generated glutathione (GSH) and subsequently in a quantitative reduction of the cellular redox environment by 2.61 mV mmol GSH per g protein. After induction of oxidative stress by using 0.78\% (v/v) ethanol, endogenous generation of ROS was consequently reduced by 24\% in resveratrol pre-treated cells. In contrast to the common perception that resveratrol acts mainly as a chemical antioxidant or as a target protein-specific ligand, we propose that the cellular response to resveratrol treatment is essentially based on oxidative triggering. In physiological microenvironments this molecular training can lead to hormetic shifting of cellular defense towards a more reductive state to improve physiological resilience to oxidative stress.},
  language  = {en}
}