@article{YeAmbiOlguinNavaetal.2021, author = {Ye, Liqing and Ambi, Uddhav B. and Olguin-Nava, Marco and Gribling-Burrer, Anne-Sophie and Ahmad, Shazeb and Bohn, Patrick and Weber, Melanie M. and Smyth, Redmond P.}, title = {RNA structures and their role in selective genome packaging}, series = {Viruses}, volume = {13}, journal = {Viruses}, number = {9}, issn = {1999-4915}, doi = {10.3390/v13091788}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-246101}, year = {2021}, abstract = {To generate infectious viral particles, viruses must specifically select their genomic RNA from milieu that contains a complex mixture of cellular or non-genomic viral RNAs. In this review, we focus on the role of viral encoded RNA structures in genome packaging. We first discuss how packaging signals are constructed from local and long-range base pairings within viral genomes, as well as inter-molecular interactions between viral and host RNAs. Then, how genome packaging is regulated by the biophysical properties of RNA. Finally, we examine the impact of RNA packaging signals on viral evolution.}, language = {en} } @article{VanHauteDietmannKremeretal.2016, author = {Van Haute, Lindsey and Dietmann, Sabine and Kremer, Laura and Hussain, Shobbir and Pearce, Sarah F. and Powell, Christopher A. and Rorbach, Joanna and Lantaff, Rebecca and Blanco, Sandra and Sauer, Sascha and Kotzaeridou, Urania and Hoffmann, Georg F. and Memari, Yasin and Kolb-Kokocinski, Anja and Durbin, Richard and Mayr, Johannes A. and Frye, Michaela and Prokisch, Holger and Minczuk, Michal}, title = {Deficient methylation and formylation of mt-tRNA(Met) wobble cytosine in a patient carrying mutations in NSUN3}, series = {Nature Communications}, volume = {7}, journal = {Nature Communications}, doi = {10.1038/ncomms12039}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-165998}, pages = {12039}, year = {2016}, abstract = {Epitranscriptome modifications are required for structure and function of RNA and defects in these pathways have been associated with human disease. Here we identify the RNA target for the previously uncharacterized 5-methylcytosine (m5C) methyltransferase NSun3 and link m5C RNA modifications with energy metabolism. Using whole-exome sequencing, we identified loss-of-function mutations in NSUN3 in a patient presenting with combined mitochondrial respiratory chain complex deficiency. Patient-derived fibroblasts exhibit severe defects in mitochondrial translation that can be rescued by exogenous expression of NSun3. We show that NSun3 is required for deposition of m5C at the anticodon loop in the mitochondrially encoded transfer RNA methionine (mt-tRNAMet). Further, we demonstrate that m5C deficiency in mt-tRNAMet results in the lack of 5-formylcytosine (f5C) at the same tRNA position. Our findings demonstrate that NSUN3 is necessary for efficient mitochondrial translation and reveal that f5C in human mitochondrial RNA is generated by oxidative processing of m5C.}, language = {en} }