@article{MoellerOverloeperFoerstneretal.2014,
  author    = {M{\"o}ller, Philip and Overl{\"o}per, Aaron and F{\"o}rstner, Konrad U. and Wen, Tuan-Nan and Sharma, Cynthia M. and Lai, Erh-Min and Narberhaus, Franz},
  title     = {Profound Impact of Hfq on Nutrient Acquisition, Metabolism and Motility in the Plant Pathogen Agrobacterium tumefaciens},
  series = {PLOS ONE},
  volume    = {9},
  journal   = {PLOS ONE},
  number    = {10},
  doi       = {10.1371/journal.pone.0110427},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-114874},
  pages     = {e110427},
  year      = {2014},
  abstract  = {As matchmaker between mRNA and sRNA interactions, the RNA chaperone Hfq plays a key role in riboregulation of many bacteria. Often, the global influence of Hfq on the transcriptome is reflected by substantially altered proteomes and pleiotropic phenotypes in hfq mutants. Using quantitative proteomics and co-immunoprecipitation combined with RNA-sequencing (RIP-seq) of Hfq-bound RNAs, we demonstrate the pervasive role of Hfq in nutrient acquisition, metabolism and motility of the plant pathogen Agrobacterium tumefaciens. 136 of 2544 proteins identified by iTRAQ (isobaric tags for relative and absolute quantitation) were affected in the absence of Hfq. Most of them were associated with ABC transporters, general metabolism and motility. RIP-seq of chromosomally encoded Hfq 3xFlag revealed 1697 mRNAs and 209 non-coding RNAs (ncRNAs) associated with Hfq. 56 ncRNAs were previously undescribed. Interestingly, 55\% of the Hfq-bound ncRNAs were encoded antisense (as) to a protein-coding sequence suggesting that A. tumefaciens Hfq plays an important role in asRNA-target interactions. The exclusive enrichment of 296 mRNAs and 31 ncRNAs under virulence conditions further indicates a role for post-transcriptional regulation in A. tumefaciens-mediated plant infection. On the basis of the iTRAQ and RIP-seq data, we assembled a comprehensive model of the Hfq core regulon in A. tumefaciens.},
  language  = {en}
}
@article{GruenwaldFastMuelleretal.2014,
  author    = {Gr{\"u}nwald, Stefanie and Fast, Anna and M{\"u}ller, Karen and Boll, Michael and Kler, Adolf and Bonnl{\"a}nder, Bernd and Wenzel, Uwe},
  title     = {Feeding a grape seed extract extends the survival of the red flour beetle Tribolium castaneum under heat-stress depending on nrf-2, jnk-1, and foxo-1 homologous genes but independent of catechin monomers},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-101089},
  year      = {2014},
  abstract  = {Besides caloric restriction, a diet rich in fruits and vegetables is believed to delay the ageing process thus providing a powerfull tool in preventive medicine. To investigate underlying interactions between food ingredients and genes simple models, such as the red flour beetle Tribolium castaneum, appear especially constructive. Here we show that 1 \% of a grape seed extract containing 30 \% of procyanidins, significantly increases the survival of T. castaneum at 42 °C when added to flour as a dietary source. The beneficial effects of grape seed extract could not be reproduced by supplementing flour with single catechins of which the oligomeric procyanidins consist. We identified previously stress resistance genes responsible for a survival extension by dietary ingredients and show here by the use of RNA-interference that a knockdown of transcripts encoding homologues of Nrf-2 or Jnk-1 block the effects of grape seed extract on survival. Interestingly, grape seed extract under knockdown of Foxo-1 caused a significant survival reduction, stressing the hormetic response as underlying the survival extension by the dietary interventions. In conclusion, our studies provide evidence that a procyanidin-rich extract is able to extend the survival of the model organism T. castaneum. Catechin monomers, however, appear not to mediate the effects. The active ingredients, moreover, need the presence of stress resistance factors, and here especially of Foxo-1, in order to promote their preventive activities with regard to degenerations.},
  subject      = {Langlebigkeit},
  language  = {en}
}