@article{SchartlKneitzWildeetal.2012,
  author    = {Schartl, Manfred and Kneitz, Susanne and Wilde, Brigitta and Wagner, Toni and Henkel, Christiaan V. and Spaink, Hermann P. and Meierjohann, Svenja},
  title     = {Conserved expression signatures between medaka and human pigment cell tumors},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-75848},
  year      = {2012},
  abstract  = {Aberrations in gene expression are a hallmark of cancer cells. Differential tumor-specific transcript levels of single genes or whole sets of genes may be critical for the neoplastic phenotype and important for therapeutic considerations or useful as biomarkers. As an approach to filter out such relevant expression differences from the plethora of changes noted in global expression profiling studies, we searched for changes of gene expression levels that are conserved. Transcriptomes from massive parallel sequencing of different types of melanoma from medaka were generated and compared to microarray datasets from zebrafish and human melanoma. This revealed molecular conservation at various levels between fish models and human tumors providing a useful strategy for identifying expression signatures strongly associated with disease phenotypes and uncovering new melanoma molecules.},
  subject      = {Biologie},
  language  = {en}
}
@article{AlbrechtSharmaReinhardtetal.2010,
  author    = {Albrecht, Marco and Sharma, Cynthia M. and Reinhardt, Richard and Vogel, Joerg and Rudel, Thomas},
  title     = {Deep sequencing-based discovery of the Chlamydia trachomatis transcriptome},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-68389},
  year      = {2010},
  abstract  = {Chlamydia trachomatis is an obligate intracellular pathogenic bacterium that has been refractory to genetic manipulations. Although the genomes of several strains have been sequenced, very little information is available on the gene structure of these bacteria. We used deep sequencing to define the transcriptome of purified elementary bodies (EB) and reticulate bodies (RB) of C. trachomatis L2b, respectively. Using an RNAseq approach, we have mapped 363 transcriptional start sites (TSS) of annotated genes. Semiquantitative analysis of mapped cDNA reads revealed differences in the RNA levels of 84 genes isolated from EB and RB, respectively. We have identified and in part confirmed 42 genome- and 1 plasmid-derived novel non-coding RNAs. The genome encoded non-coding RNA, ctrR0332 was one of the most abundantly and differentially expressed RNA in EB and RB, implying an important role in the developmental cycle of C. trachomatis. The detailed map of TSS in a thus far unprecedented resolution as a complement to the genome sequence will help to understand the organization, control and function of genes of this important pathogen.},
  subject      = {Biologie},
  language  = {en}
}
@article{KreftBurgerGoebel1983,
  author    = {Kreft, J{\"u}rgen and Burger, Klaus J. and Goebel, Werner},
  title     = {Expression of antibiotic resistance genes from Escherichia coli in Bacillus subtilis},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60600},
  year      = {1983},
  abstract  = {Bifunctional recombinant plasmids were constructed, comprised of the E. coli vectors pBR322, pBR325 and pACYC184 and different plasmids from Gram-positive bacteria, e.g. pBSU161-1 of B. subtilis and pUB110 and pC221 of S. aureus. The beta-lactamase (bla) gene and the chloramphenicol acetyltransferase (cat) gene from the E. coli plasmids were not transcribed and therefore not expressed in B. subtilis. However, tetracycline resistance from the E. coli plasmids was expressed in B. subtilis. Transcription of the tetracycline resistance gene(s) started in B. subtilis at or near the original E. coli promoter, the sequence of which is almost identical with the sequence recognized by σ<sup>55</sup> of B. subtilis RNA polymerase.},
  subject      = {Biologie},
  language  = {en}
}
@article{GoebMeyerNatusBenaventeetal.2011,
  author    = {G{\"o}b, Eva and Meyer-Natus, Elisabeth and Benavente, Ricardo and Alsheimer, Manfred},
  title     = {Expression of individual mammalian Sun1 isoforms depends on the cell type},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-68750},
  year      = {2011},
  abstract  = {Mammalian Sun1 belongs to an evolutionarily conserved family of inner nuclear membrane proteins, which are known as SUN domain proteins. SUN domain proteins interact with KASH domain partners to form bridging complexes, so-called LINC complexes, that physically connect the nuclear interior to the cytoskeleton. LINC complexes are critical for nuclear integrity and play fundamental roles in nuclear positioning, shaping and movement. The mammalian genome codes for at least five different SUN domain proteins used for the formation of a number of different LINC complexes. Recently, we reported on the identification of everal Sun1 isoforms, which tremendously enlarges the alternatives to form functional LINC complexes. We now confirmed that Sun1 actually exists in at least seven distinct splice variants. Besides that, we observed that expression of individual Sun1 isoforms remarkably depends on the cell type, suggesting a cell type-specific adaption of Sun1 dependent LINC complexes to specific cellular and physiological requirements.},
  subject      = {Biologie},
  language  = {en}
}
@article{SturmHessWeibeletal.2012,
  author    = {Sturm, Julia B. and Hess, Michael and Weibel, Stephanie and Chen, Nanhei G. and Yu, Yong A. and Zhang, Quian and Donat, Ulrike and Reiss, Cora and Gambaryan, Stepan and Krohne, Georg and Stritzker, Jochen and Szalay, Aladar A.},
  title     = {Functional hyper-IL-6 from vaccinia virus-colonized tumors triggers platelet formation and helps to alleviate toxicity of mitomycin C enhanced virus therapy},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-75224},
  year      = {2012},
  abstract  = {Background: Combination of oncolytic vaccinia virus therapy with conventional chemotherapy has shown promise for tumor therapy. However, side effects of chemotherapy including thrombocytopenia, still remain problematic. Methods: Here, we describe a novel approach to optimize combination therapy of oncolytic virus and chemotherapy utilizing virus-encoding hyper-IL-6, GLV-1h90, to reduce chemotherapy-associated side effects. Results: We showed that the hyper-IL-6 cytokine was successfully produced by GLV-1h90 and was functional both in cell culture as well as in tumor-bearing animals, in which the cytokine-producing vaccinia virus strain was well tolerated. When combined with the chemotherapeutic mitomycin C, the anti-tumor effect of the oncolytic virotherapy was significantly enhanced. Moreover, hyper-IL-6 expression greatly reduced the time interval during which the mice suffered from chemotherapy-induced thrombocytopenia. Conclusion: Therefore, future clinical application would benefit from careful investigation of additional cytokine treatment to reduce chemotherapy-induced side effects.},
  subject      = {Biologie},
  language  = {en}
}
@article{GoebelKathariouKuhnetal.1988,
  author    = {Goebel, Werner and Kathariou, S. and Kuhn, M. and Sokolovic, Z. and Kreft, J{\"u}rgen and K{\"o}hler, S. and Funke, D. and Chakraborty, T. and Leimeister-W{\"a}chter, M.},
  title     = {Hemolysin from Listeria-biochemistry, genetics and function in pathogenesis},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60563},
  year      = {1988},
  abstract  = {No abstract available},
  subject      = {Biologie},
  language  = {en}
}
@article{PrustyBoehmeBergmannetal.2012,
  author    = {Prusty, Bhupesh K. and B{\"o}hme, Linda and Bergmann, Birgit and Siegl, Christine and Krause, Eva and Mehlitz, Adrian and Rudel, Thomas},
  title     = {Imbalanced oxidative stress causes chlamydial persistence during non-productive Human Herpes Virus co-infection},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-76215},
  year      = {2012},
  abstract  = {Both human herpes viruses and Chlamydia are highly prevalent in the human population and are detected together in different human disorders. Here, we demonstrate that co-infection with human herpes virus 6 (HHV6) interferes with the developmental cycle of C. trachomatis and induces persistence. Induction of chlamydial persistence by HHV6 is independent of productive virus infection, but requires the interaction and uptake of the virus by the host cell. On the other hand, viral uptake is strongly promoted under co-infection conditions. Host cell glutathione reductase activity was suppressed by HHV6 causing NADPH accumulation, decreased formation of reduced glutathione and increased oxidative stress. Prevention of oxidative stress restored infectivity of Chlamydia after HHV6-induced persistence. We show that co-infection with Herpes simplex virus 1 or human Cytomegalovirus also induces chlamydial persistence by a similar mechanism suggesting that Chlamydia -human herpes virus co-infections are evolutionary shaped interactions with a thus far unrecognized broad significance.},
  subject      = {Biologie},
  language  = {en}
}
@article{HaasDumbskyKreft1992,
  author    = {Haas, Albert and Dumbsky, Martina and Kreft, J{\"u}rgen},
  title     = {Listeriolysin genes: complete sequence of ilo from Listeria ivanovii and of lso from Listeria seeligeri},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60529},
  year      = {1992},
  abstract  = {The completc DNA scqucnccs coding for thc thiol-activated cytolysins from Listeria ivanovii, ivanolysin 0 (ILO) and for sccligerolysin 0 (LSO) from Listeria seeligeri have been dctermined. Thc deduced amino acid scquences revealed that: (i) the primary translation products comprise 528 (ILO) and 530 (LSO) amino acids. respectively. (ii) ILO contains two cysteines. LSO has a substitution in the conserved cysteine motif.},
  subject      = {Biologie},
  language  = {en}
}
@article{MenescalSchmidtLiedtkeetal.2012,
  author    = {Menescal, Luciana and Schmidt, Cornelia and Liedtke, Daniel and Schartl, Manfred},
  title     = {Liver hyperplasia after tamoxifen induction of Myc in a transgenic medaka model},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-75316},
  year      = {2012},
  abstract  = {Myc is a global transcriptional regulator and one of the most frequently overexpressed oncoproteins in human tumors. It is well established that activation of Myc leads to enhanced cell proliferation but can also lead to increased apoptosis. The use of animal models expressing deregulated levels of Myc has helped to both elucidate its function in normal cells and give insight into how Myc initiates and maintains tumorigenesis. Analyses of the medaka (Oryzias latipes) genome uncovered the unexpected presence of two Myc gene copies in this teleost species. Comparison of these Myc versions to other vertebrate species revealed that one gene, myc17, differs by the loss of some conserved regulatory protein motifs present in all other known Myc genes. To investigate how such differences might affect the basic biological functions of Myc, we generated a tamoxifeninducible in vivo model utilizing a natural, fish-specific Myc gene. Using this model we show that, when activated, Myc17 leads to increased proliferation and to apoptosis in a dose-dependent manner, similar to human Myc. We have also shown that long-term Myc17 activation triggers liver hyperplasia in adult fish, allowing this newly established transgenic medaka model to be used to study the transition from hyperplasia to liver cancer and to identify Myc-induced tumorigenesis modifiers.},
  subject      = {Biologie},
  language  = {en}
}
@article{RatzkaFoersterLiangetal.2012,
  author    = {Ratzka, Carolin and F{\"o}rster, Frank and Liang, Chunguang and Kupper, Maria and Dandekar, Thomas and Feldhaar, Heike and Gross, Roy},
  title     = {Molecular characterization of antimicrobial peptide genes of the carpenter ant Camponotus floridanus},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-75985},
  year      = {2012},
  abstract  = {The production of antimicrobial peptides (AMPs) is a major defense mechanism against pathogen infestation and of particular importance for insects relying exclusively on an innate immune system. Here, we report on the characterization of three AMPs from the carpenter ant Camponotus floridanus. Due to sequence similarities and amino acid composition these peptides can be classified into the cysteine-rich (e.g. defensin) and glycine-rich (e.g. hymenoptaecin) AMP groups, respectively. The gene and cDNA sequences of these AMPs were established and their expression was shown to be induced by microbial challenge. We characterized two different defensin genes. The defensin-2 gene has a single intron, whereas the defensin-1 gene has two introns. The deduced amino acid sequence of the C. floridanus defensins is very similar to other known ant defensins with the exception of a short C-terminal extension of defensin-1. The hymenoptaecin gene has a single intron and a very peculiar domain structure. The corresponding precursor protein consists of a signal- and a pro-sequence followed by a hymenoptaecin-like domain and six directly repeated hymenoptaecin domains. Each of the hymenoptaecin domains is flanked by an EAEP-spacer sequence and a RR-site known to be a proteolytic processing site. Thus, proteolytic processing of the multipeptide precursor may generate several mature AMPs leading to an amplification of the immune response. Bioinformatical analyses revealed the presence of hymenoptaecin genes with similar multipeptide precursor structure in genomes of other ant species suggesting an evolutionary conserved important role of this gene in ant immunity.},
  subject      = {Biologie},
  language  = {en}
}