@article{SchilcherHilsmannRauscheretal.2021,
  author    = {Schilcher, Felix and Hilsmann, Lioba and Rauscher, Lisa and Değirmenci, Laura and Krischke, Markus and Krischke, Beate and Ankenbrand, Markus and Rutschmann, Benjamin and Mueller, Martin J. and Steffan-Dewenter, Ingolf and Scheiner, Ricarda},
  title     = {In vitro rearing changes social task performance and physiology in honeybees},
  series = {Insects},
  volume    = {13},
  journal   = {Insects},
  number    = {1},
  issn      = {2075-4450},
  doi       = {10.3390/insects13010004},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-252305},
  year      = {2021},
  abstract  = {In vitro rearing of honeybee larvae is an established method that enables exact control and monitoring of developmental factors and allows controlled application of pesticides or pathogens. However, only a few studies have investigated how the rearing method itself affects the behavior of the resulting adult honeybees. We raised honeybees in vitro according to a standardized protocol: marking the emerging honeybees individually and inserting them into established colonies. Subsequently, we investigated the behavioral performance of nurse bees and foragers and quantified the physiological factors underlying the social organization. Adult honeybees raised in vitro differed from naturally reared honeybees in their probability of performing social tasks. Further, in vitro-reared bees foraged for a shorter duration in their life and performed fewer foraging trips. Nursing behavior appeared to be unaffected by rearing condition. Weight was also unaffected by rearing condition. Interestingly, juvenile hormone titers, which normally increase strongly around the time when a honeybee becomes a forager, were significantly lower in three- and four-week-old in vitro bees. The effects of the rearing environment on individual sucrose responsiveness and lipid levels were rather minor. These data suggest that larval rearing conditions can affect the task performance and physiology of adult bees despite equal weight, pointing to an important role of the colony environment for these factors. Our observations of behavior and metabolic pathways offer important novel insight into how the rearing environment affects adult honeybees.},
  language  = {en}
}
@article{HesselbachSeegerSchilcheretal.2020,
  author    = {Hesselbach, Hannah and Seeger, Johannes and Schilcher, Felix and Ankenbrand, Markus and Scheiner, Ricarda},
  title     = {Chronic exposure to the pesticide flupyradifurone can lead to premature onset of foraging in honeybees Apis mellifera},
  series = {Journal of Applied Ecology},
  volume    = {57},
  journal   = {Journal of Applied Ecology},
  number    = {3},
  doi       = {10.1111/1365-2664.13555},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-212769},
  pages     = {609-618},
  year      = {2020},
  abstract  = {1.Honeybees Apis mellifera and other pollinating insects suffer from pesticides in agricultural landscapes. Flupyradifurone is the active ingredient of a novel pesticide by the name of 'Sivanto', introduced by Bayer AG (Crop Science Division, Monheim am Rhein, Germany). It is recommended against sucking insects and marketed as 'harmless' to honeybees. Flupyradifurone binds to nicotinergic acetylcholine receptors like neonicotinoids, but it has a different mode of action. So far, little is known on how sublethal flupyradifurone doses affect honeybees. 2. We chronically applied a sublethal and field-realistic concentration of flupyradifurone to test for long-term effects on flight behaviour using radio-frequency identification. We examined haematoxylin/eosin-stained brains of flupyradifurone-treated bees to investigate possible changes in brain morphology and brain damage. 3. A field-realistic flupyradifurone dose of approximately 1.0 μg/bee/day significantly increased mortality. Pesticide-treated bees initiated foraging earlier than control bees. No morphological damage in the brain was observed. 4. Synthesis and applications. The early onset of foraging induced by a chronical application of flupyradifurone could be disadvantageous for honeybee colonies, reducing the period of in-hive tasks and life expectancy of individuals. Radio-frequency identification technology is a valuable tool for studying pesticide effects on lifetime foraging behaviour of insects.},
  language  = {en}
}
@article{BemmBeckerLarischetal.2016,
  author    = {Bemm, Felix and Becker, Dirk and Larisch, Christina and Kreuzer, Ines and Escalante-Perez, Maria and Schulze, Waltraud X. and Ankenbrand, Markus and Van de Weyer, Anna-Lena and Krol, Elzbieta and Al-Rasheid, Khaled A. and Mith{\"o}fer, Axel and Weber, Andreas P. and Schultz, J{\"o}rg and Hedrich, Rainer},
  title     = {Venus flytrap carnivorous lifestyle builds on herbivore defense strategies},
  series = {Genome Research},
  volume    = {26},
  journal   = {Genome Research},
  number    = {6},
  doi       = {10.1101/gr.202200.115},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-188799},
  pages     = {812-825},
  year      = {2016},
  abstract  = {Although the concept of botanical carnivory has been known since Darwin's time, the molecular mechanisms that allow animal feeding remain unknown, primarily due to a complete lack of genomic information. Here, we show that the transcriptomic landscape of the Dionaea trap is dramatically shifted toward signal transduction and nutrient transport upon insect feeding, with touch hormone signaling and protein secretion prevailing. At the same time, a massive induction of general defense responses is accompanied by the repression of cell death-related genes/processes. We hypothesize that the carnivory syndrome of Dionaea evolved by exaptation of ancient defense pathways, replacing cell death with nutrient acquisition.},
  language  = {en}
}
@article{WolfChenSongetal.2013,
  author    = {Wolf, Matthias and Chen, Shilin and Song, Jingyuan and Ankenbrand, Markus and M{\"u}ller, Tobias},
  title     = {Compensatory Base Changes in ITS2 Secondary Structures Correlate with the Biological Species Concept Despite Intragenomic Variability in ITS2 Sequences - A Proof of Concept},
  series = {PLoS ONE},
  journal   = {PLoS ONE},
  doi       = {10.1371/journal.pone.0066726},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-96450},
  year      = {2013},
  abstract  = {Compensatory base changes (CBCs) in internal transcribed spacer 2 (ITS2) rDNA secondary structures correlate with Ernst Mayr's biological species concept. This hypothesis also referred to as the CBC species concept recently was subjected to large-scale testing, indicating two distinct probabilities. (1) If there is a CBC then there are two different species with a probability of ~0.93. (2) If there is no CBC then there is the same species with a probability of ~0.76. In ITS2 research, however, the main problem is the multicopy nature of ITS2 sequences. Most recently, 454 pyrosequencing data have been used to characterize more than 5000 intragenomic variations of ITS2 regions from 178 plant species, demonstrating that mutation of ITS2 is frequent, with a mean of 35 variants per species, respectively per individual organism. In this study, using those 454 data, the CBC criterion is reconsidered in the light of intragenomic variability, a proof of concept, a necessary criterion, expecting no intragenomic CBCs in variant ITS2 copies. In accordance with the CBC species concept, we could demonstrate that the probability that there is no intragenomic CBC is ~0.99.},
  language  = {en}
}