@article{GoebelKathariouKuhnetal.1988, author = {Goebel, Werner and Kathariou, S. and Kuhn, M. and Sokolovic, Z. and Kreft, J{\"u}rgen and K{\"o}hler, S. and Funke, D. and Chakraborty, T. and Leimeister-W{\"a}chter, M.}, title = {Hemolysin from Listeria-biochemistry, genetics and function in pathogenesis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60563}, year = {1988}, abstract = {No abstract available}, subject = {Biologie}, language = {en} } @article{KreftFunkeSchlesingeretal.1989, author = {Kreft, J{\"u}rgen and Funke, D. and Schlesinger, R. and Lottspeich, F. and Goebel, Werner}, title = {Purification and characterization of cytolysins from Listeria monocytogenes serovar 4b and Listeria ivanovii}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-47036}, year = {1989}, abstract = {Several exoproteins from Listeria monocytogenes serovar 4b (NCTC 10527) and Listeria ivanovii (ATCC) 19119, SLCC 2379), respectively, have been purified to homogeneity by thiol-disulfide exchange chromatography and gel filtration. Both strains produce a haemolytic/cytolytic protein of Mr 58 kDa, which has all the properties of a SH-activated cytolysin, the prototype of which is streptolysin 0 (SLO), and this protein has therefore heen termed Iisteriolysin 0 (LLO). In addition a protein of Mr 24 kDa from culture supernatants of L. ivanovii co-purified withLLO. The N-terminal aminoacid sequences of both proteins from L. ivanovii have been determined. By mutagenesis with transposons of Gram-positive origin (Tn916 and TnI545), which have been introduced via conjugation into L. ivanovii, several phenotypic mutants (altered haemolysis on sheep blood agar or lecithinase-negative) were obtained. Results on the properties of these muntants will he presented.}, language = {en} }