@article{BarnekowGessler1986, author = {Barnekow, Angelika and Gessler, Manfred}, title = {Activation of the pp60\(^{c-src}\) kinase during differentiation of monomyelocytic cells in vitro}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59278}, year = {1986}, abstract = {Tbe proto-oncogene c-src, the cellular homolog of the Rous sarcoma virus (RSV) transforming gene v-src, is expressed in a tissue-specific and age-dependent manner. Its physiological function, although still unknown, appears to be more closely related to differentiation processes than to proliferation processes. To obtain more information about the physiological role of the c-src gene in cells, we have studied differentiation-dependent alterations using the human HL-60 leukaemia cell line as a model system. Induction of monocytic and granulocytic differentiation of HL-60 cells by 12-0-tetradecanoylphorbol-13-acetate (TPA) and dimethylsulfoxide (DMSO) is associated with an activation of the pp60c-src tyrosine kinase, but not with increased c-src gene expression. Control experiments exclude an interaction of TPA and DMSO themselves with the pp60c-src kinase.}, subject = {Biochemie}, language = {en} } @article{Dandekar1986, author = {Dandekar, Thomas}, title = {Offenlegungsschrift ({\"u}ber einen Biosensor)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31683}, year = {1986}, abstract = {No abstract available}, language = {de} } @article{GabelliniSebald1986, author = {Gabellini, N. and Sebald, Walter}, title = {Nucleotide sequence and transcription of the fbc operon from Rhodopseudomonas sphaeroides. Evaluation of the deduced amino acid sequences of the FeS protein, cytochrome b and cytochrome c\(_1\)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62615}, year = {1986}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{HadjiolovaRoseScheer1986, author = {Hadjiolova, Krassimira and Rose, Kathleen M. and Scheer, Ulrich}, title = {Immunolocalization of nucleolar proteins after D-galactosamine-induced inhibition of transcription in rat hepatocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33205}, year = {1986}, abstract = {No abstract available}, language = {en} } @article{HoppeGattiWeberetal.1986, author = {Hoppe, J. and Gatti, D. and Weber, H. and Sebald, Walter}, title = {Labeling of individual amino acid residues in the membrane-embedded F\(_0\) part of the F\(_1\) F\(_0\) ATP synthase from Neurospora crassa. Influence of oligomycin and dicyclohexylcarbodiimide}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62598}, year = {1986}, abstract = {Three F0 subunits and the F\(_1\) subunit P of the ATP synthase from Neurospora crassa were labeled with the lipophilic photoactivatable reagent 3-(trifluoromethyl)-3-(m-[\(^{125}\)I]iodophenyl)diazirine ([\(^{125}\)I]TID). In the proteolipid subunit which was the most heavily labeled polypeptide labeling was confmed to five residues at the NH2-terminus and five residues at the C-terminus ofthe protein. Labeling occurred at similar positions compared with the homologaus protein (subunit c) in the ATP synthase from Escherichia coli, indicating a similar structure of the proteolipid subunits in their respective organisms. The inhibitors oligomycin and dicyclohexylcarbodiimide did not change the pattern of accessible surface residues in the proteolipid, suggesting that neither inhibitor induces gross conformational changes. However, in the presence of oligomycin, the extent oflabeling in some residues was reduced. Apparently, these residues provide part of the binding site for the inhibitor. After reaction with dicyclohexylcarbodiimide an additional labeled amino acid was found at position 65 corresponding to the invariant carbod{\"u}mide-binding glutamic acid. These results and previous observations indicate that the carboxyl side chain of Glu-65 is located at the protein-lipid interphase. The idea is discussed that proton translocation occurs at the interphase between different types if F\(_0\) subunits. Dicyclohexylcarbodiimide or oligomycin might disturb this essential interaction between the F\(_0\) subunits.}, subject = {Biochemie}, language = {en} } @article{HoppeSebald1986, author = {Hoppe, J. and Sebald, Walter}, title = {Topological studies suggest that the pathway of the protons through F\(_0\) is provided by amino acid residues accessible from the lipid phase}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62602}, year = {1986}, abstract = {The structure of the F0 part of ATP synthases from E. coli and Neurospora crassa was analyzed by hydrophobic surface labeling with [125I]TID. In the E. co/i F0 all three subunits were freely accessible to the reagent, suggesting that these subunits are independently integrated in the membrane. Labeted amino acid residues were identified by Edman degradation of the dicyclohexylcarbodiimide binding (DCCD) proteins from E. coli and Neurospora crassa. The very similar patterns obtained with the two homologaus proteins suggested the existence of tightly packed cx-helices. The oligomeric structure of the DCCD binding protein appeared to be very rigid since little, if any, change in the labeling patternwas observed upon addition of oligomycin or DCCD to membranes from Neurospora crassa. When membrancs were pretrcated with DCCD prior to the reaction with [125I]TID an additionally labeled amino acid appeared at the position of Glu·65 which binds DCCD covalently, indicating the Jocation of this inhibitor on the outside of the oligomer. It is suggested that proton conduction occurs at the surface of the oligomer of the DCCD binding protein. Possibly this oligomer rotates against the subunit a or b and thus enables proton translocation. Conserved residues in subunit a, probably located in the Iipid bilayer, might participate in the pro· ton translocation mechanism.}, subject = {Biochemie}, language = {en} } @article{KobeltLinsenmair1986, author = {Kobelt, Frank and Linsenmair, Karl Eduard}, title = {Adaptations of the reed frog Hyperolius viridiflavus to its arid environment. I. The skin of Hyperolius viridiflavus nitidulus in wet and dry season conditions.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30551}, year = {1986}, abstract = {Hyperolius viridiflavus nitidulus inhabits parts of the seasonally very hot and dry West African savanna. During the long lasting dry season, the small frog is sitting unhidden on mostly dry plants and has to deal with high solar radiation load (SRL), evaporative water loss (EWL) and small energy reserves. It seems to be very badly equipped to survive such harsh climatic conditions (unfavorable surface to volume ratio, very limited capacity to st{\"o}re energy and water). Therefore, it must have developed extraordinary efficient mechanisms to solve the mentioned Problems. Some of these mechanisms are to be looked for within the skin of the animal (e.g. protection against fast desiccation, deleterious effects of UV radiation and over-heating). The morphology of the wet season skin is, in most aspects, that of a "normal" anuran skin. It differs in the Organization of the processes of the melanophores and in the arrangement of the chromatophores in the Stratum spongiosum, forming no "Dermal Chromatophore Unit". During the adaptation to dry season conditions the number of iridophores in dorsal and ventral skin is increased 4-6 times compared to wet season skin. This increase is accompanied by a very conspicuous change of the wet season color pattern. Now, at air temperatures below 35° C the color becomes brownish white or grey and changes to a brilliant white at air temperatures near and over 40° C. Thus, in dry season State the frog retains its ability for rapid color change. In wet season State the platelets of the iridophores are irregularly distributed. In dry season State many platelets become arranged almost parallel to the surface. These purine crystals probably act as quarter-wave-length interference reflectors, reducing SRL by reflecting a considerable amount of the radiated energy input. EWL is as low as that of much larger xeric reptilians. The impermeability of the skin seems to be the result of several mechanisms (ground substance, iridophores, lipids, mucus) supplementing each other. The light red skin at the pelvic region and inner sides of the limbs is specialized for rapid uptake of water allowing the frog to replenish the unavoidable EWL by using single drops of dew or rain, available for only very short periods.}, language = {en} } @article{Linsenmair1986, author = {Linsenmair, Karl Eduard}, title = {Adaptations of the reed frog Hyperbolius viridiflavus to its arid environment: II. Some aspects of the water economy of H. viridiflavus nitidulus under wet and dry ...}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-78395}, year = {1986}, abstract = {Adaptations to aridity ofthe reedfrog Hyperolius viridiflavus nitidulus, living in different parts of the seasonally very dry and hot West African savanna, are investigated ...}, subject = {Zoologie}, language = {en} } @article{Mahsberg1986, author = {Mahsberg, Dieter}, title = {Contact chemoreception of prey in hunting scorpions}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45784}, year = {1986}, abstract = {Scorpions commonly are assumed to hunt on living prey. But under laboratory conditions they also respond very sensitively to dead insects lying on the substrate. In many cases the motionless prey is seized and consumed. It was investigated how this behavior can be elicited. The buthid scorpions Androctonus australis (L.) and Buthus occitanus (Am.) not only find motionless prey again which was stung but managed to escape before dying: They also respond to extracts of the cuticle of prey insects. After touching prey marks' either with the tips of the chelae fingers or the tarsi of the walking legs or the pectine organs specific responses (searching, seizing, feeding) are released at a high rate. Behavioral experiments demonstrate for the first time the chemosensitivity of the pectine organs for which only mechanosensitivity had been proofed formerly. Mechanical as well as contact chemical stimulation of these organs cause scorpions to orient towards the stimulus source which is grasped, retained and consumed or rejected depending on its quality. The probably responsible chemosensitive receptors are already described in the literature. The possible adaptive value and the biological significance of contact chemoreception in prey catching and in other aspects of the life of scorpions is discussed.}, subject = {Skorpion}, language = {en} } @article{McCarthySebaldGrossetal.1986, author = {McCarthy, J. E. and Sebald, Walter and Gross, G. and Lammers, R.}, title = {Enhancement of translational efficiency by the Escherichia coli atpE translational initiation region: its fusion with two human genes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62626}, year = {1986}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} }