@article{RasaNoraKrukleHenningetal.2018,
  author    = {Rasa, Santa and Nora-Krukle, Zaiga and Henning, Nina and Eliassen, Eva and Shikova, Evelina and Harrer, Thomas and Scheibenbogen, Carmen and Murovska, Modra and Prusty, Bhupesh K.},
  title     = {Chronic viral infections in myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS)},
  series = {Journal of Translational Medicine},
  volume    = {16},
  journal   = {Journal of Translational Medicine},
  number    = {268},
  doi       = {10.1186/s12967-018-1644-y},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-224960},
  pages     = {1-25},
  year      = {2018},
  abstract  = {Background and main text: Myalgic encephalomyelitis/chronic fatigue syndrome (ME/CFS) is a complex and controversial clinical condition without having established causative factors. Increasing numbers of cases during past decade have created awareness among patients as well as healthcare professionals. Chronic viral infection as a cause of ME/CFS has long been debated. However, lack of large studies involving well-designed patient groups and validated experimental set ups have hindered our knowledge about this disease. Moreover, recent developments regarding molecular mechanism of pathogenesis of various infectious agents cast doubts over validity of several of the past studies. Conclusions: This review aims to compile all the studies done so far to investigate various viral agents that could be associated with ME/CFS. Furthermore, we suggest strategies to better design future studies on the role of viral infections in ME/CFS.},
  language  = {en}
}
@article{KasimirToomeyLiuetal.2022,
  author    = {Kasimir, Francesca and Toomey, Danny and Liu, Zheng and Kaiping, Agnes C. and Ariza, Maria Eugenia and Prusty, Bhupesh K.},
  title     = {Tissue specific signature of HHV-6 infection in ME/CFS},
  series = {Frontiers in Molecular Biosciences},
  volume    = {9},
  journal   = {Frontiers in Molecular Biosciences},
  issn      = {2296-889X},
  doi       = {10.3389/fmolb.2022.1044964},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-299433},
  year      = {2022},
  abstract  = {First exposure to various human herpesviruses (HHVs) including HHV-6, HCMV and EBV does not cause a life-threatening disease. In fact, most individuals are frequently unaware of their first exposure to such pathogens. These herpesviruses acquire lifelong latency in the human body where they show minimal genomic activity required for their survival. We hypothesized that it is not the latency itself but a timely, regionally restricted viral reactivation in a sub-set of host cells that plays a key role in disease development. HHV-6 (HHV-6A and HHV-6B) and HHV-7 are unique HHVs that acquire latency by integration of the viral genome into sub-telomeric region of human chromosomes. HHV-6 reactivation has been linked to Alzheimer's Disease, Chronic Fatigue Syndrome, and many other diseases. However, lack of viral activity in commonly tested biological materials including blood or serum strongly suggests tissue specific localization of active HHV-6 genome. Here in this paper, we attempted to analyze active HHV-6 transcripts in postmortem tissue biopsies from a small cohort of ME/CFS patients and matched controls by fluorescence in situ hybridization using a probe against HHV-6 microRNA (miRNA), miR-aU14. Our results show abundant viral miRNA in various regions of the human brain and associated neuronal tissues including the spinal cord that is only detected in ME/CFS patients and not in controls. Our findings provide evidence of tissue-specific active HHV-6 and EBV infection in ME/CFS, which along with recent work demonstrating a possible relationship between herpesvirus infection and ME/CFS, provide grounds for renewed discussion on the role of herpesviruses in ME/CFS.},
  language  = {en}
}