@article{ZilligPauliWieseretal.2023, author = {Zillig, Anna-Lena and Pauli, Paul and Wieser, Matthias and Reicherts, Philipp}, title = {Better safe than sorry? - On the influence of learned safety on pain perception}, series = {PloS One}, volume = {18}, journal = {PloS One}, number = {11}, doi = {10.1371/journal.pone.0289047}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-349905}, year = {2023}, abstract = {The experience of threat was found to result—mostly—in increased pain, however it is still unclear whether the exact opposite, namely the feeling of safety may lead to a reduction of pain. To test this hypothesis, we conducted two between-subject experiments (N = 94; N = 87), investigating whether learned safety relative to a neutral control condition can reduce pain, while threat should lead to increased pain compared to a neutral condition. Therefore, participants first underwent either threat or safety conditioning, before entering an identical test phase, where the previously conditioned threat or safety cue and a newly introduced visual cue were presented simultaneously with heat pain stimuli. Methodological changes were performed in experiment 2 to prevent safety extinction and to facilitate conditioning in the first place: We included additional verbal instructions, increased the maximum length of the ISI and raised CS-US contingency in the threat group from 50\% to 75\%. In addition to pain ratings and ratings of the visual cues (threat, safety, arousal, valence, and contingency), in both experiments, we collected heart rate and skin conductance. Analysis of the cue ratings during acquisition indicate successful threat and safety induction, however results of the test phase, when also heat pain was administered, demonstrate rapid safety extinction in both experiments. Results suggest rather small modulation of subjective and physiological pain responses following threat or safety cues relative to the neutral condition. However, exploratory analysis revealed reduced pain ratings in later trials of the experiment in the safety group compared to the threat group in both studies, suggesting different temporal dynamics for threat and safety learning and extinction, respectively. Perspective: The present results demonstrate the challenge to maintain safety in the presence of acute pain and suggest more research on the interaction of affective learning mechanism and pain processing.}, language = {en} } @article{ZiebellRodriguesForsteretal.2023, author = {Ziebell, Philipp and Rodrigues, Johannes and Forster, Andr{\´e} and Sanguinetti, Joseph L. and Allen, John JB. and Hewig, Johannes}, title = {Inhibition of midfrontal theta with transcranial ultrasound explains greater approach versus withdrawal behavior in humans}, series = {Brain Stimulation}, volume = {16}, journal = {Brain Stimulation}, number = {5}, doi = {10.1016/j.brs.2023.08.011}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-349890}, pages = {1278-1288}, year = {2023}, abstract = {Highlights • Transcranial ultrasound neuromodulation/stimulation (TUS) is a growing field. • We conducted a double-blind sham-controlled within-subjects large sample TUS study. • Right prefrontal cortex TUS inhibits midfrontal theta electroencephalography (MFT). • TUS MFT inhibition explains greater approach versus withdrawal in a virtual T-maze. • This distinct TUS-MFT-behavior link merits future basic and applied research. Abstract Recent reviews highlighted low-intensity transcranial focused ultrasound (TUS) as a promising new tool for non-invasive neuromodulation in basic and applied sciences. Our preregistered double-blind within-subjects study (N = 152) utilized TUS targeting the right prefrontal cortex, which, in earlier work, was found to positively enhance self-reported global mood, decrease negative states of self-reported emotional conflict (anxiety/worrying), and modulate related midfrontal functional magnetic resonance imaging activity in affect regulation brain networks. To further explore TUS effects on objective physiological and behavioral variables, we used a virtual T-maze task that has been established in prior studies to measure motivational conflicts regarding whether participants execute approach versus withdrawal behavior (with free-choice responses via continuous joystick movements) while allowing to record related electroencephalographic data such as midfrontal theta activity (MFT). MFT, a reliable marker of conflict representation on a neuronal level, was of particular interest to us since it has repeatedly been shown to explain related behavior, with relatively low MFT typically preceding approach-like risky behavior and relatively high MFT typically preceding withdrawal-like risk aversion. Our central hypothesis is that TUS decreases MFT in T-maze conflict situations and thereby increases approach and reduces withdrawal. Results indicate that TUS led to significant MFT decreases, which significantly explained increases in approach behavior and decreases in withdrawal behavior. This study expands TUS evidence on a physiological and behavioral level with a large sample size of human subjects, suggesting the promise of further research based on this distinct TUS-MFT-behavior link to influence conflict monitoring and its behavioral consequences. Ultimately, this can serve as a foundation for future clinical work to establish TUS interventions for emotional and motivational mental health.}, language = {en} } @phdthesis{Zhou2023, author = {Zhou, Yang}, title = {The Exploitation of Opsin-based Optogenetic Tools for Application in Higher Plants}, doi = {10.25972/OPUS-23696}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-236960}, school = {Universit{\"a}t W{\"u}rzburg}, year = {2023}, abstract = {The discovery, heterologous expression, and characterization of channelrhodopsin-2 (ChR2) - a light-sensitive cation channel found in the green alga Chlamydomonas reinhardtii - led to the success of optogenetics as a powerful technology, first in neuroscience. ChR2 was employed to induce action potentials by blue light in genetically modified nerve cells. In optogenetics, exogenous photoreceptors are expressed in cells to manipulate cellular activity. These photoreceptors were in the beginning mainly microbial opsins. During nearly two decades, many microbial opsins and their mutants were explored for their application in neuroscience. Until now, however, the application of optogenetics to plant studies is limited to very few reports. Several optogenetic strategies for plant research were demonstrated, in which most attempts are based on non-opsin optogenetic tools. Opsins need retinal (vitamin A) as a cofactor to generate the functional protein, the rhodopsin. As most animals have eyes that contain animal rhodopsins, they also have the enzyme - a 15, 15'-Dioxygenase - for retinal production from food-supplied provitamin A (beta-carotene). However, higher plants lack a similar enzyme, making it difficult to express functional rhodopsins successfully in plants. But plant chloroplasts contain plenty of beta-carotene. I introduced a gene, coding for a 15, 15'-Dioxygenase with a chloroplast target peptide, to tobacco plants. This enzyme converts a molecule of β-carotene into two of all-trans-retinal. After expressing this enzyme in plants, the concentration of all-trans-retinal was increased greatly. The increased retinal concentration led to increased expression of several microbial opsins, tested in model higher plants. Unfortunately, most opsins were observed intracellularly and not in the plasma membrane. To improve their localization in the plasma membrane, some reported signal peptides were fused to the N- or C-terminal end of opsins. Finally, I helped to identify three microbial opsins -- GtACR1 (a light-gated anion channel), ChR2 (a light-gated cation channel), PPR (a light-gated proton pump) which express and work well in the plasma membrane of plants. The transgene plants were grown under red light to prevent activation of the expressed opsins. Upon illumination with blue or green light, the activation of these opsins then induced the expected change of the membrane potential, dramatically changing the phenotype of plants with activated rhodopsins. This study is the first which shows the potential of microbial opsins for optogenetic research in higher plants, using the ubq10 promoter for ubiquitous expression. I expect this to be just the beginning, as many different opsins and tissue-specific promoters for selective expression now can be tested for their usefulness. It is further to be expected that the here established method will help investigators to exploit more optogenetic tools and explore the secrets, kept in the plant kingdom.}, language = {en} } @phdthesis{Zhang2023, author = {Zhang, Yanxiang}, title = {The Making of a Place: Topographical Literature on West Lake by Tian Rucheng (b. 1501) and Zhang Dai (b. 1597)}, doi = {10.25972/OPUS-32759}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-327590}, school = {Universit{\"a}t W{\"u}rzburg}, year = {2023}, abstract = {This dissertation explores the local gazetteers of West Lake that were compiled by literati of the Ming dynasty. In 1547, the first West Lake gazetteer was published by the local literatus of Hangzhou, Tian Rucheng 田汝成. In the late sixteenth and early seventeenth centuries, accompanying the huge enthusiasm for West Lake and the flourishing of its tourism, the production of West Lake gazetteers reached its peak. This trend, however, was reduced by the turmoils in the last years of the Ming and the dynastic transition, a period when West Lake had also experienced destruction. Nevertheless, the practice was resumed in the first decades of the Qing dynasty by some literati who had survived the disasters. One prominent work of this period was compiled by the Ming loyalist and "remnant subject" Zhang Dai 張岱, who wrote an author's preface in 1671. This dissertation can be divided into two parts. The first part focuses on the editorial principles of compilers, e.g., which materials are included, how they are organized and presented. It explores various possible intentions of the compilers, such as scholarly and documentary, practical and oriented toward tour-guiding, didactic and educational, and personal and nostalgic ones. The second part focuses on some of the perceptions, attitudes, and values of literati focusing on West Lake. The discourses analyzed in this part include West Lake as a hybrid between metropolitan city and sheer wilderness, as a national symbol and object of nostalgia of the lost dynasty, and as a place of pleasure-seeking and indulgence. While a discourse often had a long tradition and historical development, the emphasis of the study is on the late sixteenth and early seventeenth centuries, i.e., the late Ming.}, language = {en} } @misc{Zhang2023, type = {Master Thesis}, author = {Zhang, Shenxishuai}, title = {Conflicts and Anxieties over Money in Late Ming Vernacular Stories}, doi = {10.25972/OPUS-31673}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-316733}, school = {Universit{\"a}t W{\"u}rzburg}, year = {2023}, abstract = {The present study discusses money and conflicts and anxiety over money in late Ming vernacular stories and contextualizes these stories in the contemporary society of economic prosperity and rapid changes. The high monetization and extensive use of silver and copper cash as currency brought both wealth and conflicts in various aspects of society. Eleven vernacular stories from several collections are adopted as source materials for the close examination, including Jingshi tongyan (Stories to Caution the World, 1624) and Xingshi hengyan (Stories to Awaken the World, 1627) by Feng Menglong (1574-1646) and the two Pai'an jingqi (Slapping the Table in Amazement, 1628 and 1632) collections by Ling Mengchu (1580-1644), etc. The analysis then focuses on the relationship between money and four topics, the late Ming context, social relations, gender ideals, and religion. Multiple voices and various viewpoints in these narratives show human beings' struggles in taming and dominating money, the increasingly familiar and essential object in everyday life. Generally, when people cannot control money properly, there is a fear of its detrimental power to humans and social relations within and beyond families. On the contrary, characters, who are able to control money, are praised.}, subject = {Geld}, language = {en} } @article{ZeinerSchroederMetzneretal.2023, author = {Zeiner, Carsten and Schr{\"o}der, Malte and Metzner, Selina and Herrmann, Johannes and Notz, Quirin and Hottenrott, Sebastian and R{\"o}der, Daniel and Meybohm, Patrick and Lepper, Philipp M. and Lotz, Christopher}, title = {High-dose methylprednisolone pulse therapy during refractory COVID-19 acute respiratory distress syndrome: a retrospective observational study}, series = {BMC Pulmonary Medicine}, volume = {23}, journal = {BMC Pulmonary Medicine}, doi = {10.1186/s12890-023-02664-5}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-357231}, year = {2023}, abstract = {Background Current COVID-19 guidelines recommend the early use of systemic corticoids for COVID-19 acute respiratory distress syndrome (ARDS). It remains unknown if high-dose methylprednisolone pulse therapy (MPT) ameliorates refractory COVID-19 ARDS after many days of mechanical ventilation or rapid deterioration with or without extracorporeal membrane oxygenation (ECMO). Methods This is a retrospective observational study. Consecutive patients with COVID-19 ARDS treated with a parenteral high-dose methylprednisolone pulse therapy at the intensive care units (ICU) of two University Hospitals between January 1st 2021 and November 30st 2022 were included. Clinical data collection was at ICU admission, start of MPT, 3-, 10- and 14-days post MPT. Results Thirty-seven patients (mean age 55 ± 12 years) were included in the study. MPT started at a mean of 17 ± 12 days after mechanical ventilation. Nineteen patients (54\%) received ECMO support when commencing MPT. Mean paO2/FiO2 significantly improved 3- (p = 0.034) and 10 days (p = 0.0313) post MPT. The same applied to the necessary FiO2 10 days after MPT (p = 0.0240). There were no serious infectious complications. Twenty-four patients (65\%) survived to ICU discharge, including 13 out of 20 (65\%) needing ECMO support. Conclusions Late administration of high-dose MPT in a critical subset of refractory COVID-19 ARDS patients improved respiratory function and was associated with a higher-than-expected survival of 65\%. These data suggest that high-dose MPT may be a viable salvage therapy in refractory COVID-19 ARDS.}, language = {en} } @article{ZeigermannEttelt2023, author = {Zeigermann, Ulrike and Ettelt, Stefanie}, title = {Spanning the boundaries between policy, politics and science to solve wicked problems: policy pilots, deliberation fora and policy labs}, series = {Sustainability Science}, volume = {18}, journal = {Sustainability Science}, number = {2}, doi = {10.1007/s11625-022-01187-y}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-324806}, pages = {809-821}, year = {2023}, abstract = {Current crises have highlighted the importance of integrating research, politics and practice to work on solutions for complex social problems. In recent years, policy deliberation fora, policy pilots and policy labs have increasingly been deployed to mobilise science to produce solutions, help create popular support and guide implementation of policies addressing major public policy problems. Yet, we know little about how these approaches manage to transcend the boundaries between research, politics and practice. By systematically comparing policy deliberation fora, policy pilots and policy labs, this paper explores their mechanisms of boundary spanning including relationship and trust building, knowledge translation and developing solutions. We situate our analysis in healthcare policy and climate change policy in Germany, two contrasting policy fields that share a perpetual and escalating sense of crisis. Our findings suggest that deliberation fora, policy pilots and policy labs address different dilemmas of policymaking, namely the idea dilemma, the implementation dilemma and the legitimacy dilemma. All three approaches reduce wicked problems to a manageable scale, by grounding them in local decision-making, reducing their scope or reducing the problem analytically. We argue that despite their ambition to modernise democratic practices, unless they are institutionally well embedded, their effects are likely to be small scale, local and temporary.}, language = {en} } @phdthesis{Zapf2023, author = {Zapf, Ludwig}, title = {Novel Borane- and Phosphorane- Functionalized Anionic Carbene Ligands}, doi = {10.25972/OPUS-32078}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-320781}, school = {Universit{\"a}t W{\"u}rzburg}, year = {2023}, abstract = {N-heterocyclic carbenes (NHC) are utilized for the stabilization of reactive compounds, for the activation of strong bonds, and as ligands in transition metal chemistry. In contrast to neutral NHCs, few examples of anionic or even dianionic NHCs are known. One approach for the synthesis of anionic carbenes is the deprotonation of neutral or anionic precursors, bearing Lewis acids instead of alkyl or aryl substituents. Following this strategy, novel anionic and dianionic NHCs, featuring weakly coordinating fluorinated borane and phosphorane substituents or coordinating tricyanoborane substituents were synthesized within the scope of this thesis. These carbenes possess unprecedented stabilities compared to related species. Furthermore, their electronic and steric properties can be directly adjusted by the type of Lewis acid attached. Their potential as ligands with highly shielding weakly coordinating substituents next to the carbene coordination center was demonstrated by the syntheses of the respective NHC selenium adducts and NHC gold(I) complexes. In contrast anionic NHCs with coordinating tricyanoborane moieties have an outstanding potential as ditopic ligands with coordination being possible at the carbene center and via the cyano groups. Their beneficial ligand properties were demonstrated by the syntheses of the respective NHC selenium adducts and NHC nickeltricarbonyl complexes. The combination of electronic properties, the large buried volume, the negative charge, the possibility to act as ditopic or ligands with weakly coordinating groups, and the ease of accessibility render borane- and phosphorane functionalized NHCs unique novel ligands. A further project of this PhD thesis deals with the steric properties of Lewis acids. Therefore, an easy-to-apply model was designed to quantify the steric demand of Lewis acids. Using the results of this evaluation, a second model was developed which judges the steric repulsion in Lewis acid/base adduct formation for arbitrary sets of acids and bases.}, subject = {Komplexe}, language = {en} } @article{Zakaria2023, author = {Zakaria, Nevine Nizar}, title = {Assessing the working practices and the inclusive programs to students with disabilities in the Egyptian museums}, series = {Frontiers in Education}, volume = {8}, journal = {Frontiers in Education}, issn = {2504-284X}, doi = {10.3389/feduc.2023.1111695}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-319848}, year = {2023}, abstract = {Following the implementation of 2018's laws on the rights of persons with disabilities (PWDs) in Egypt, students with disabilities (SWDs) have both legal and moral rights to meaningful learning opportunities and inclusive education. Despite that, SWDs still have very limited education resources which limit their career aspirations and quality of life. In this respect, education whether as part of formal education or lifelong learning is central to the museum's mission. Museums, as part of non-formal education, are being acknowledged for their educative powers and investments in the development of quality formal, non-formal, and informal learning experiences. Further, phrases such as "inclusivity," "accessibility," and "diversity" were notably included in the newly approved museum definition by ICOM (2022) emphasizing museums' obligations to embrace societal issues and shape a cultural attitude concerning disability rights, diversity, and equality together with overcoming exclusionary educational practices. The study seeks to investigate the existing resources and inclusive practices in Egyptian museums to achieve non-formal education for SWDs. Qualitative research approaches have been employed to answer a specific question: How can Egyptian museums work within their governing systems to support the learning of SWDs beyond their formal education system? The study aims to assess the potential of Egyptian museums in facilitating learning for SWDs. Further, it examines the capability of Egyptian museums in contributing to informal and non-formal learning for SWDs and striving for inclusive education inspired by the social model of disability that fosters inclusive educational programs and adopts a human rights-based approach. The results revealed that Egyptian museums contributed to the learning of SWDs, yet small-scale programs and individual efforts, but they are already engaged in active inclusive practices that address the learning of SWDs. The study suggests that they need to be acknowledged and supported by the government as state instruments and direct actors in advancing inclusive education and implementing appropriate pedagogies in favor of SWDs.}, language = {en} } @article{ZaitsevaHoffmannLoestetal.2023, author = {Zaitseva, Olena and Hoffmann, Annett and L{\"o}st, Margaretha and Anany, Mohamed A. and Zhang, Tengyu and Kucka, Kirstin and Wiegering, Armin and Otto, Christoph and Wajant, Harald}, title = {Antibody-based soluble and membrane-bound TWEAK mimicking agonists with FcγR-independent activity}, series = {Frontiers in Immunology}, volume = {14}, journal = {Frontiers in Immunology}, doi = {10.3389/fimmu.2023.1194610}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-323116}, year = {2023}, abstract = {Fibroblast growth factor (FGF)-inducible 14 (Fn14) activates the classical and alternative NFκB (nuclear factor 'kappa-light-chain-enhancer' of activated B-cells) signaling pathway but also enhances tumor necrosis factor (TNF)-induced cell death. Fn14 expression is upregulated in non-hematopoietic cells during tissue injury and is also often highly expressed in solid cancers. In view of the latter, there were and are considerable preclinical efforts to target Fn14 for tumor therapy, either by exploiting Fn14 as a target for antibodies with cytotoxic activity (e.g. antibody-dependent cellular cytotoxicity (ADCC)-inducing IgG variants, antibody drug conjugates) or by blocking antibodies with the aim to interfere with protumoral Fn14 activities. Noteworthy, there are yet no attempts to target Fn14 with agonistic Fc effector function silenced antibodies to unleash the proinflammatory and cell death-enhancing activities of this receptor for tumor therapy. This is certainly not at least due to the fact that anti-Fn14 antibodies only act as effective agonists when they are presented bound to Fcγ receptors (FcγR). Thus, there are so far no antibodies that robustly and selectively engage Fn14 signaling without triggering unwanted FcγR-mediated activities. In this study, we investigated a panel of variants of the anti-Fn14 antibody 18D1 of different valencies and domain architectures with respect to their inherent FcγR-independent ability to trigger Fn14-associated signaling pathways. In contrast to conventional 18D1, the majority of 18D1 antibody variants with four or more Fn14 binding sites displayed a strong ability to trigger the alternative NFκB pathway and to enhance TNF-induced cell death and therefore resemble in their activity soluble (TNF)-like weak inducer of apoptosis (TWEAK), one form of the natural occurring ligand of Fn14. Noteworthy, activation of the classical NFκB pathway, which naturally is predominately triggered by membrane-bound TWEAK but not soluble TWEAK, was preferentially observed with a subset of constructs containing Fn14 binding sites at opposing sites of the IgG scaffold, e.g. IgG1-scFv fusion proteins. A superior ability of IgG1-scFv fusion proteins to trigger classical NFκB signaling was also observed with the anti-Fn14 antibody PDL192 suggesting that we identified generic structures for Fn14 antibody variants mimicking soluble and membrane-bound TWEAK.}, language = {en} }