@article{SchmidSteinlein2016,
  author    = {Schmid, Michael and Steinlein, Claus},
  title     = {Chromosome Banding in Amphibia. XXXIII. Demonstration of 5-Methylcytosine-Rich Heterochromatin in Anura},
  series = {Cytogenetic and Genome Research},
  volume    = {148},
  journal   = {Cytogenetic and Genome Research},
  number    = {1},
  issn      = {1424-8581},
  doi       = {10.1159/000446141},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-199022},
  pages     = {35-43},
  year      = {2016},
  abstract  = {An experimental approach using monoclonal anti-5-methylcytosine (5-MeC) antibodies and indirect immunofluorescence was elaborated for detecting 5-MeC-rich chromosome regions in anuran chromosomes. This technique was applied to mitotic metaphases of 6 neotropical frog species belonging to 6 genera and 4 families. The hypermethylation patterns were compared with a variety of banding patterns obtained by conventional banding techniques. The hypermethylated DNA sequences are species-specific and located exclusively in constitutive heterochromatin. They are found in centromeric, pericentromeric, telomeric, and interstitial positions of the chromosomes and adjacent to nucleolus organizer regions. 5-MeC-rich DNA sequences can be embedded both in AT- and GC-rich repetitive DNA. The experimental parameters that have major influence on the reproducibility and quality of the anti-5-MeC antibody labeling are discussed.},
  language  = {en}
}