@article{KunzRuehlingMoldovanetal.2021,
  author    = {Kunz, Tobias C. and R{\"u}hling, Marcel and Moldovan, Adriana and Paprotka, Kerstin and Kozjak-Pavlovic, Vera and Rudel, Thomas and Fraunholz, Martin},
  title     = {The Expandables: Cracking the Staphylococcal Cell Wall for Expansion Microscopy},
  series = {Frontiers in Cellular and Infection Microbiology},
  volume    = {11},
  journal   = {Frontiers in Cellular and Infection Microbiology},
  issn      = {2235-2988},
  doi       = {10.3389/fcimb.2021.644750},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-232292},
  year      = {2021},
  abstract  = {Expansion Microscopy (ExM) is a novel tool improving the resolution of fluorescence microscopy by linking the sample into a hydrogel that gets physically expanded in water. Previously, we have used ExM to visualize the intracellular Gram-negative pathogens Chlamydia trachomatis, Simkania negevensis, and Neisseria gonorrhoeae. Gram-positive bacteria have a rigid and thick cell wall that impedes classic expansion strategies. Here we developed an approach, which included a series of enzymatic treatments resulting in isotropic 4× expansion of the Gram-positive pathogen Staphylococcus aureus. We further demonstrate the suitability of the technique for imaging of planktonic bacteria as well as endocytosed, intracellular bacteria at a spatial resolution of approximately 60 nm with conventional confocal laser scanning microscopy.},
  language  = {en}
}
@article{KunzGoetzSaueretal.2019,
  author    = {Kunz, Tobias C. and G{\"o}tz, Ralph and Sauer, Markus and Rudel, Thomas},
  title     = {Detection of chlamydia developmental forms and secreted effectors by expansion microscopy},
  series = {Frontiers in Cellular and Infection Microbiology},
  volume    = {9},
  journal   = {Frontiers in Cellular and Infection Microbiology},
  number    = {276},
  issn      = {2235-2988},
  doi       = {10.3389/fcimb.2019.00276},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-195716},
  year      = {2019},
  abstract  = {Expansion microscopy (ExM) is a novel tool to improve the resolution of fluorescence-based microscopy that has not yet been used to visualize intracellular pathogens. Here we show the expansion of the intracellular pathogen Chlamydia trachomatis, enabling to differentiate its two distinct forms, catabolic active reticulate bodies (RB) and infectious elementary bodies (EB), on a conventional confocal microscope. We show that ExM enables the possibility to precisely locate chlamydial effector proteins, such as CPAF or Cdu1, within and outside of the chlamydial inclusion. Thus, we claim that ExM offers the possibility to address a broad range of questions and may be useful for further research on various intracellular pathogens.},
  language  = {en}
}