@article{ArtyukovaGorboulevRodionovaetal.1985, author = {Artyukova, E. V. and Gorboulev, Valentin G. and Rodionova, N. P. and Krylov, A. V. and Atabekov, J. G.}, title = {Comparative study of structural peculiarities and translation of potexvirus RNAs}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46985}, year = {1985}, abstract = {Structural peculiarities of the S'-end segments of genomic RNA were studied in F potato virus (F-PV) and white clover mosaic virus (WCMV). The methods of affinity chromatography on oligo(dT) cellulose and oligonucleotide mapping revealed a prolonged (up to 210 nucleotides) polyadenyl sequence at the 3'-end of F-PV RNA. A polyadenyl sequence is missing at the 3'end of WCMV RNA. A study of the translation products of WCMV and F-PV RNAs in a oe11-free protein-synthesizing system derived from rabbit reticulocytes showed that polypeptides electrophoretically comigrating with a structural protein of either virus were synthesized alongside high-molecular-weight polypeptides (M\(_r\)\(\approx\) 180-150 kdaltons).}, language = {en} } @article{RubtsovChernovGorboulevetal.1985, author = {Rubtsov, P. M. and Chernov, V. G. and Gorboulev, Valentin G. and Parsadanyan, A. S. and Sverdlova, P. S. and Chupeeva, V. V. and Golova, Yu. B. and Batchikova, N. V. and Zvirblis, G. S. and Skryabin, K. G. and Bayev, A. A.}, title = {Genetic engineering of peptide hormones}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46964}, year = {1985}, abstract = {Peptide and polypeptide hormones represent an extensive group of biologically active compounds of important significance for medicine and agriculture. In recent years genetic engineering methods have been used to create strains of microorganisms synthesizing eukaryotic proteins, including hormones and their precursors. The first stage of such developments is the isolation of DNA coding the des~red product. We have accomplished the cloning of the cDNA of a number of polypeptide and peptide hormones of the pituitary of man and domestic animals. The model gene of human calcitonin has also been synthesized and cloned. The obtained genes are being used to develop methods for the microbiological synthesis of human and animal-hormones.}, language = {en} }