@article{KodererSchmitzWuenschetal.2022,
  author    = {Koderer, Corinna and Schmitz, Werner and W{\"u}nsch, Anna Chiara and Balint, Julia and El-Mesery, Mohamed and Volland, Julian Manuel and Hartmann, Stefan and Linz, Christian and K{\"u}bler, Alexander Christian and Seher, Axel},
  title     = {Low energy status under methionine restriction is essentially independent of proliferation or cell contact inhibition},
  series = {Cells},
  volume    = {11},
  journal   = {Cells},
  number    = {3},
  issn      = {2073-4409},
  doi       = {10.3390/cells11030551},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-262329},
  year      = {2022},
  abstract  = {Nonlimited proliferation is one of the most striking features of neoplastic cells. The basis of cell division is the sufficient presence of mass (amino acids) and energy (ATP and NADH). A sophisticated intracellular network permanently measures the mass and energy levels. Thus, in vivo restrictions in the form of amino acid, protein, or caloric restrictions strongly affect absolute lifespan and age-associated diseases such as cancer. The induction of permanent low energy metabolism (LEM) is essential in this process. The murine cell line L929 responds to methionine restriction (MetR) for a short time period with LEM at the metabolic level defined by a characteristic fingerprint consisting of the molecules acetoacetate, creatine, spermidine, GSSG, UDP-glucose, pantothenate, and ATP. Here, we used mass spectrometry (LC/MS) to investigate the influence of proliferation and contact inhibition on the energy status of cells. Interestingly, the energy status was essentially independent of proliferation or contact inhibition. LC/MS analyses showed that in full medium, the cells maintain active and energetic metabolism for optional proliferation. In contrast, MetR induced LEM independently of proliferation or contact inhibition. These results are important for cell behaviour under MetR and for the optional application of restrictions in cancer therapy.},
  language  = {en}
}
@phdthesis{Haschke2021,
  author    = {Haschke, Sebastian},
  title     = {Untersuchung Thiol-En vernetzter Gelatine Hydrogele und Vergleich mit Alginat-Gelatine in Bezug auf das in vitro Zellverhalten von Fibroblasten},
  doi       = {10.25972/OPUS-24872},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-248727},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2021},
  abstract  = {Hydrogele stehen als Material f{\"u}r den 3D-Biodruck zunehmend im Fokus aktueller Forschung, da sie aufgrund ihrer wasserhaltigen Struktur optimale Voraussetzungen f{\"u}r Anwendungen der Zellkultur aufweisen. Durch die Verarbeitung solcher Biotinten mittels additiver Fertigungstechniken der Biofabrikation erhofft man sich besch{\"a}digtes oder krankes Gewebe zu heilen oder zu ersetzen. Allerdings wird der Fortschritt in diesem Bereich durch einen Mangel an geeigneten Materialien gebremst, weshalb die Entwicklung neuer Biotinten von zentraler Bedeutung ist. Das Polymer GelAGE ist ein am Lehrstuhl f{\"u}r Funktionswerkstoffe der Medizin und Zahnheilkunde der Universit{\"a}t W{\"u}rzburg synthetisiertes Hydrogelsystem. Zu diesem {\"u}ber eine Thiol-En Reaktion vernetzenden Material stehen systematische Untersuchungen der f{\"u}r die in vitro Zellkultur relevanten Eigenschaften noch aus. Das Ziel dieser Arbeit war daher die biologische Evaluation von GelAGE und der Vergleich mit der Biotinte Alginat-Gelatine. Zu diesem Zweck wurden L929-Zellen f{\"u}r 7 Tage in verschiedenen Hydrogelzusammensetzungen in vitro kultiviert. Um die zytokompatiblen Eigenschaften in den verschiedenen Versuchsgruppen zu untersuchen, wurden die Proben mittels der in vitro Testverfahren Live/Dead F{\"a}rbung, DNA-Assay, CCK-8-Assay und Phalloidin-F{\"a}rbung analysiert. Im Rahmen dieser Arbeit konnte ein Herstellungsprotokoll f{\"u}r das Material GelAGE etabliert werden, welches eine Grundlage f{\"u}r die Durchf{\"u}hrung weiterer biologischer Experimente bietet. Das Resultat der biologischen Untersuchungen war, dass das Polymer GelAGE als zytokompatibel bewertet werden kann, es jedoch nicht die Qualit{\"a}t des Alginat-Gelatine Hydrogelsystems aufweist. Allerdings konnten die Eigenschaften der GelAGE Proben teilweise durch eine Modifikation mit Humanem Pl{\"a}ttchenlysat verbessert werden. Des Weiteren konnten deutliche Unterschiede in der Zell-Material- Interaktion zwischen den verschiedenen GelAGE Varianten nachgewiesen werden.},
  subject      = {Hydrogel},
  language  = {de}
}
@article{SchmitzKodererElMeseryetal.2021,
  author    = {Schmitz, Werner and Koderer, Corinna and El-Mesery, Mohamed and Gobik, Sebastian and Sampers, Rene and Straub, Anton and K{\"u}bler, Alexander Christian and Seher, Axel},
  title     = {Metabolic fingerprinting of murine L929 fibroblasts as a cell-based tumour suppressor model system for methionine restriction},
  series = {International Journal of Molecular Sciences},
  volume    = {22},
  journal   = {International Journal of Molecular Sciences},
  number    = {6},
  issn      = {1422-0067},
  doi       = {10.3390/ijms22063039},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-259198},
  year      = {2021},
  abstract  = {Since Otto Warburg reported in 1924 that cancer cells address their increased energy requirement through a massive intake of glucose, the cellular energy level has offered a therapeutic anticancer strategy. Methionine restriction (MetR) is one of the most effective approaches for inducing low-energy metabolism (LEM) due to the central position in metabolism of this amino acid. However, no simple in vitro system for the rapid analysis of MetR is currently available, and this study establishes the murine cell line L929 as such a model system. L929 cells react rapidly and efficiently to MetR, and the analysis of more than 150 different metabolites belonging to different classes (amino acids, urea and tricarboxylic acid cycle (TCA) cycles, carbohydrates, etc.) by liquid chromatography/mass spectrometry (LC/MS) defines a metabolic fingerprint and enables the identification of specific metabolites representing normal or MetR conditions. The system facilitates the rapid and efficient testing of potential cancer therapeutic metabolic targets. To date, MS studies of MetR have been performed using organisms and yeast, and the current LC/MS analysis of the intra- and extracellular metabolites in the murine cell line L929 over a period of 5 days thus provides new insights into the effects of MetR at the cellular metabolic level.},
  language  = {en}
}