@article{EneLohseVladuetal.2016,
  author    = {Ene, Iuliana V. and Lohse, Matthew B. and Vladu, Adrian V. and Morschh{\"a}user, Joachim and Johnson, Alexander D. and Bennett, Richard J.},
  title     = {Phenotypic Profiling Reveals that Candida albicans Opaque Cells Represent a Metabolically Specialized Cell State Compared to Default White Cells},
  series = {mBio},
  volume    = {7},
  journal   = {mBio},
  number    = {6},
  doi       = {10.1128/mBio.01269-16},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-165818},
  pages     = {e01269-16},
  year      = {2016},
  abstract  = {The white-opaque switch is a bistable, epigenetic transition affecting multiple traits in Candida albicans including mating, immunogenicity, and niche specificity. To compare how the two cell states respond to external cues, we examined the fitness, phenotypic switching, and filamentation properties of white cells and opaque cells under 1,440 different conditions at 25°C and 37°C. We demonstrate that white and opaque cells display striking differences in their integration of metabolic and thermal cues, so that the two states exhibit optimal fitness under distinct conditions. White cells were fitter than opaque cells under a wide range of environmental conditions, including growth at various pHs and in the presence of chemical stresses or antifungal drugs. This difference was exacerbated at 37°C, consistent with white cells being the default state of C. albicans in the mammalian host. In contrast, opaque cells showed greater fitness than white cells under select nutritional conditions, including growth on diverse peptides at 25°C. We further demonstrate that filamentation is significantly rewired between the two states, with white and opaque cells undergoing filamentous growth in response to distinct external cues. Genetic analysis was used to identify signaling pathways impacting the white-opaque transition both in vitro and in a murine model of commensal colonization, and three sugar sensing pathways are revealed as regulators of the switch. Together, these findings establish that white and opaque cells are programmed for differential integration of metabolic and thermal cues and that opaque cells represent a more metabolically specialized cell state than the default white state.},
  language  = {en}
}