@article{JedeHenzeMeinersetal.2023,
  author    = {Jede, Christian and Henze, Laura J. and Meiners, Kirstin and Bogdahn, Malte and Wedel, Marcel and van Axel, Valeria},
  title     = {Development and application of a dissolution-transfer-partitioning system (DTPS) for biopharmaceutical drug characterization},
  series = {Pharmaceutics},
  volume    = {15},
  journal   = {Pharmaceutics},
  number    = {4},
  issn      = {1999-4923},
  doi       = {10.3390/pharmaceutics15041069},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-311149},
  year      = {2023},
  abstract  = {A variety of in vitro dissolution and gastrointestinal transfer models have been developed aiming to predict drug supersaturation and precipitation. Further, biphasic, one-vessel in vitro systems are increasingly applied to simulate drug absorption in vitro. However, to date, there is a lack of combining the two approaches. Therefore, the first aim of this study was to develop a dissolution-transfer-partitioning system (DTPS) and, secondly, to assess its biopredictive power. In the DTPS, simulated gastric and intestinal dissolution vessels are connected via a peristaltic pump. An organic layer is added on top of the intestinal phase, serving as an absorptive compartment. The predictive power of the novel DTPS was assessed to a classical USP II transfer model using a BCS class II weak base with poor aqueous solubility, MSC-A. The classical USP II transfer model overestimated simulated intestinal drug precipitation, especially at higher doses. By applying the DTPS, a clearly improved estimation of drug supersaturation and precipitation and an accurate prediction of the in vivo dose linearity of MSC-A were observed. The DTPS provides a useful tool taking both dissolution and absorption into account. This advanced in vitro tool offers the advantage of streamlining the development process of challenging compounds.},
  language  = {en}
}