@article{WinterPryssProbstetal.2020,
  author    = {Winter, Michael and Pryss, R{\"u}diger and Probst, Thomas and Reichert, Manfred},
  title     = {Towards the applicability of measuring the electrodermal activity in the context of process model comprehension: feasibility study},
  series = {Sensors},
  volume    = {20},
  journal   = {Sensors},
  number    = {16},
  issn      = {1424-8220},
  doi       = {10.3390/s20164561},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-211276},
  year      = {2020},
  abstract  = {Process model comprehension is essential in order to understand the five Ws (i.e., who, what, where, when, and why) pertaining to the processes of organizations. However, research in this context showed that a proper comprehension of process models often poses a challenge in practice. For this reason, a vast body of research exists studying the factors having an influence on process model comprehension. In order to point research towards a neuro-centric perspective in this context, the paper at hand evaluates the appropriateness of measuring the electrodermal activity (EDA) during the comprehension of process models. Therefore, a preliminary test run and a feasibility study were conducted relying on an EDA and physical activity sensor to record the EDA during process model comprehension. The insights obtained from the feasibility study demonstrated that process model comprehension leads to an increased activity in the EDA. Furthermore, EDA-related results indicated significantly that participants were confronted with a higher cognitive load during the comprehension of complex process models. In addition, the experiences and limitations we learned in measuring the EDA during the comprehension of process models are discussed in this paper. In conclusion, the feasibility study demonstrated that the measurement of the EDA could be an appropriate method to obtain new insights into process model comprehension.},
  language  = {en}
}
@phdthesis{Waidelich2005,
  author    = {Waidelich, Michael},
  title     = {Neue Aspekte zum Design von ionensensitiven und solvatochromen Fluoreszenzsensoren},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-15578},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2005},
  abstract  = {Neuartige Akzeptor-substituierte Fluoresenzsensoren wurden etabliert, die durch signifikante Rotverschiebung der Emissionsmaxima Analyten nachweisen und deren pH-Sensitivit{\"a}t {\"u}ber das Substitutionsmuster variierbar ist. Es wurde gezeigt, dass 2-Methoxyanthracenderivate eine duale Emission aufweisen, die in dieser Form noch nicht detektiert und untersucht worden ist. Desweiteren wurde ein neues Strukturelement f{\"u}r stark solvatochrome Proben etabliert, die als Fluoreszenzsensoren zur Detektion von Fluorid und Analyten mit hoher Akzeptornummer verwendet werden k{\"o}nnen. Außerdem konnte eine Fluoreszenzsonde als Leucht-Sensor zur selektiven, differenzierenden Detektion von Fluorid und Chlorid generiert werden.},
  subject      = {Fluoreszenzsonde},
  language  = {de}
}
@phdthesis{Nikolaev2005,
  author    = {Nikolaev, Viacheslav},
  title     = {Development and application of fluorescent cAMP und cGMP biosensors},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-15673},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2005},
  abstract  = {The cyclic nucleotides cAMP and cGMP are two ubiquitous important second messengers, which regulate diverse physiological responses from vision and memory to blood pressure and thrombus formation. They act in cells via cAMP- and cGMP-dependent protein kinases (PKA and GK), cyclic nucleotide-gated channels and Epac. Although the concept of cyclic nucleotide signalling is well developed based on classical biochemical studies, these techniques have not allowed to analyze cAMP and cGMP in live cells with high temporal and spatial resolution. In the present study fluorescence resonance energy transfer was used to develop a technique for visualization of cAMP and cGMP in live cells and in vitro by means of fluorescent biosensors. Ligand-induced conformational change in a single nucleotide-binding domain flanked with green fluorescent protein mutants was used for dynamic, highly sensitive measurements of cAMP and cGMP. Such biosensors retained binding properties and chemical specificity of unmodified domains, allowing to image cyclic nucleotides in a physiologically relevant range of concentrations. To develop cAMP-sensors, binding domains of PKA, Epac and cAMP-gated HCN-channel were used. cGMP-sensors were based on single domains of GK and phosphodiesterases (PDEs). Sensors based on Epac were used to analyze spatio-temporal dynamics of cAMP in neurons and macrophages, demonstrating that cAMP-gradients travel with a high speed (~ 40 \&\#956;m/s) throughout the entire cytosol. To understand the mechanisms of cAMP-compartmentation, kinetics properties of phosphodi-esterase (PDE2) were, next, analyzed in aldosterone producing cells. PDE2 is able to rapidly hydrolyze extensive amounts of cAMP, so that the speed of cAMP-hydrolysis is much faster than that of its synthesis, which might serve as a basis of compartmentation. cAMP-sensors were also used to develop a clinically relevant diagnostic method for reliable detection of \&\#946;1-adrenergic receptor autoantibodies in cardiac myopathy patients, which has allowed to significantly increase the sensitivity of previously developed diagnostic approaches. Conformational change in a single binding domain of GK and PDE was, next, used to create novel fluorescent biosensors for cGMP. These sensors demonstrated high spatio-temporal resolution and were applied to analyze rapid dynamics of cGMP production by soluble and particulate guanylyl cyclases as well as to image cGMP in mesangial cells. In summary, highly sensitive biosensors for cAMP and cGMP based on single cyclic nucleotide-binding domains have been developed and used in various biological and clinically relevant applications.},
  subject      = {Cyclo-AMP},
  language  = {en}
}
@phdthesis{Loisel2004,
  author    = {Loisel, Claudine},
  title     = {Korrosionsempfindliche Dosimetermaterialien zur {\"U}berwachung der Umweltbedingungen an Kulturg{\"u}tern},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-10835},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2004},
  abstract  = {Das Ziel dieser Arbeit war es, ein neues Dosimetermaterial zu entwickeln, das schneller reagiert als der klassische Glassensor. Einen vielversprechenden Ansatz daf{\"u}r bietet der Sol-Gel Prozeß, mit dem d{\"u}nne Schichten hergestellt werden k{\"o}nnen. Erste Versuche mit transparenten Schichten einer glas{\"a}hnlichen Zusammensetzung (mit einem hohen Anteil an K und Ca) waren nicht erfolgreich, da eine deutliche Erh{\"o}hung der Reaktivit{\"a}t nicht erreicht wurde. Schichten, die einen sehr hohen Ca-Anteil aufweisen, zeigten allerdings die gew{\"u}nschte Empfindlichkeit gegen Umwelteinfl{\"u}sse. Die neuen „Rapid-Sensoren" werden aus einem vorkonsensierten SiO2-Sol (Silizium (IV) Oxid-Sol) und Ca(NO3)2\&\#61655; 4H2O in Aceton (Molverh{\"a}ltnis Ca : Si = 10 : 1) hergestellt. Objekttr{\"a}ger werden mit diesem Sol beidseitig beschichtet. Die Tauchbeschichtung und die Temperung (5 Minuten bei 600 °C) wurden auf hohe Empfindlichkeit gegen Feuchtigkeit und Schadgase (Screening Test in einer Klimakammer) optimiert. Die neuen Sensoren sind im sichtbaren Spektralbereich nicht transparent, sondern opak, k{\"o}nnen aber wie die klassischen Glassensoren mit IR-Spektroskopie (in Transmission) ausgewertet werden, wobei der Anstieg der OH-Bande bei 3300 cm-1 als Mass f{\"u}r den Korrosionsfortschritt (genannt \&\#61508;E-Wert) dient. Die aktive Sensorschicht setzt sich aus kristallinen und amorphen Bestandteilen zusammen. Die Zusammensetzung und Morphologie der Kristallphase wurde weitestgehend charakterisiert. Mit Lichtmikroskopie l{\"a}sst sich die Oberfl{\"a}che des Rapid-Sensors als eine Vielzahl kleiner polygoner Kristalle charakterisieren, f{\"u}r die im REM beobachtet verschiedene Wachstumsstufen erkennbar sind. Mit Hilfe der EDX-Analyse und ICP-AES wurden Si, O, Ca und Na als die Hauptelemente der Schicht bestimmt. Mit SNMS-Tiefenprofil konnte eine Diffusion von Na aus dem Objekttr{\"a}ger in die Schicht nachgewiesen werden, was zu einer besonders guten Haftung f{\"u}hrt. Mittels R{\"o}ntgendiffraktometrie, IR- und Raman-Spektroskopie lassen sich Informationen {\"u}ber die Struktur der Schicht erhalten: die Kristallphase besteht aus einer Mischung aus Calciumoxid und Calcium-silicat-en, die mit XRD schwer zu unterscheiden sind. Auch im infraroten Spektralbereich weisen die Si-O-Schwingungen auf silicathaltige, Bestandteile in der amorphen Schicht hin. F{\"u}r die Kalibrierung des neuen Dosimetermaterials sind Bewitterungen unter kontrollierten Bedingungen grundlegend notwendig. Dazu wurde ein Bewitterunsprogramm (I) mit hoher Feuchte und Temperatur (40 °C, 98 \% r.F.) sowie ein zweites mit Zugabe von SO2 als Schadgas (II) gew{\"a}hlt. Beide Programme beschleunigen die Umweltwirkung im Vergleich zu Realbedingungen und haben sich in anderen Versuchen mit klassischen Glassensoren bew{\"a}hrt. Zusammenfassend l{\"a}sst sich aus den Bewitterungsversuchen feststellen, dass der neue Sensor integrativ auf Temperatur, Feuchte, und Schadgas reagiert. Entsprechend der Reaktion des klassischen Sensors f{\"u}hrt eine Temperatur / Feuchte- Bewitterung zur Bildung von CaCO3-Kristallen, w{\"a}hrend bei Anwesenheit von SO2 bevorzugt Gipskristalle gebildet werden. Diese Parallelen lassen den Schluß auf ein vergleichbares Reaktionsprinzip zu, obwohl die Reaktion der Calciumsilicate, aus denen die Schicht besteht, nur bedingt mit der f{\"u}r Glas typischen Verwitterung vergleichbar ist. Mit REM kann man bei Rapid-Sensoren beobachten, dass die Reaktion am Rand der Kristalle beginnt und in die Tiefe fortschreitet, bis zur vollst{\"a}ndigen Umsetzung (S{\"a}ttigung). Die kristallinen Korrosionsprodukte breiten sich im weiteren Verlauf auch auf der amorphen Schicht aus. Der Mechanismus ist nicht reversibel und entspricht damit nicht dem f{\"u}r por{\"o}se SiO2-Schichten beschriebenen Alterungprozeß. Erste Sensorstudien unter nat{\"u}rlicher Bewitterungsbedingungen erm{\"o}glichen einen Vergleich mit klassischen Glassensoren und umreissen das k{\"u}nftige Einsatzspektrum. Expositionen in der ISC-Außenstelle Bronnbach und im Gr{\"u}nen Gew{\"o}lbe in Dresden zeigen, dass die Rapid-Sensoren schneller reagieren als klassische Glassensoren (Steigerung um etwa Faktor 3). Unter moderat korrosiven Bedingungen im Innenraum sind 4 Wochen Expositionszeit g{\"u}nstig (mindestens 3 Monate f{\"u}r Glassensoren) w{\"a}hrend im Außenraum Rapid-Sensoren innerhalb von 7 Tagen ansprechen (einige Wochen f{\"u}r herk{\"o}mmliche Glassensoren).},
  subject      = {Kulturgut},
  language  = {de}
}
@article{KruegerFriedrichFoersteretal.2012,
  author    = {Krueger, Beate and Friedrich, Torben and F{\"o}rster, Frank and Bernhardt, J{\"o}rg and Gross, Roy and Dandekar, Thomas},
  title     = {Different evolutionary modifications as a guide to rewire two-component systems},
  series = {Bioinformatics and Biology Insights},
  volume    = {6},
  journal   = {Bioinformatics and Biology Insights},
  doi       = {10.4137/BBI.S9356},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-123647},
  pages     = {97-128},
  year      = {2012},
  abstract  = {Two-component systems (TCS) are short signalling pathways generally occurring in prokaryotes. They frequently regulate prokaryotic stimulus responses and thus are also of interest for engineering in biotechnology and synthetic biology. The aim of this study is to better understand and describe rewiring of TCS while investigating different evolutionary scenarios. Based on large-scale screens of TCS in different organisms, this study gives detailed data, concrete alignments, and structure analysis on three general modification scenarios, where TCS were rewired for new responses and functions: (i) exchanges in the sequence within single TCS domains, (ii) exchange of whole TCS domains; (iii) addition of new components modulating TCS function. As a result, the replacement of stimulus and promotor cassettes to rewire TCS is well defined exploiting the alignments given here. The diverged TCS examples are non-trivial and the design is challenging. Designed connector proteins may also be useful to modify TCS in selected cases.},
  language  = {en}
}
@phdthesis{Klein2010,
  author    = {Klein, Alexander},
  title     = {Performance Issues of MAC and Routing Protocols in Wireless Sensor Networks},
  doi       = {10.25972/OPUS-4465},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-52870},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2010},
  abstract  = {The focus of this work lies on the communication issues of Medium Access Control (MAC) and routing protocols in the context of WSNs. The communication challenges in these networks mainly result from high node density, low bandwidth, low energy constraints and the hardware limitations in terms of memory, computational power and sensing capabilities of low-power transceivers. For this reason, the structure of WSNs is always kept as simple as possible to minimize the impact of communication issues. Thus, the majority of WSNs apply a simple one hop star topology since multi-hop communication has high demands on the routing protocol since it increases the bandwidth requirements of the network. Moreover, medium access becomes a challenging problem due to the fact that low-power transceivers are very limited in their sensing capabilities. The first contribution is represented by the Backoff Preamble-based MAC Protocol with Sequential Contention Resolution (BPS-MAC) which is designed to overcome the limitations of low-power transceivers. Two communication issues, namely the Clear Channel Assessment (CCA) delay and the turnaround time, are directly addressed by the protocol. The CCA delay represents the period of time which is required by the transceiver to detect a busy radio channel while the turnaround time specifies the period of time which is required to switch between receive and transmit mode. Standard Carrier Sense Multiple Access (CSMA) protocols do not achieve high performance in terms of packet loss if the traffic is highly correlated due to the fact that the transceiver is not able to sense the medium during the switching phase. Therefore, a node may start to transmit data while another node is already transmitting since it has sensed an idle medium right before it started to switch its transceiver from receive to transmit mode. The BPS-MAC protocol uses a new sequential preamble-based medium access strategy which can be adapted to the hardware capabilities of the transceivers. The protocol achieves a very low packet loss rate even in wireless networks with high node density and event-driven traffic without the need of synchronization. This makes the protocol attractive to applications such as structural health monitoring, where event suppression is not an option. Moreover, acknowledgments or complex retransmission strategies become almost unnecessary since the sequential preamble-based contention resolution mechanism minimizes the collision probability. However, packets can still be lost as a consequence of interference or other issues which affect signal propagation. The second contribution consists of a new routing protocol which is able to quickly detect topology changes without generating a large amount of overhead. The key characteristics of the Statistic-Based Routing (SBR) protocol are high end-to-end reliability (in fixed and mobile networks), load balancing capabilities, a smooth continuous routing metric, quick adaptation to changing network conditions, low processing and memory requirements, low overhead, support of unidirectional links and simplicity. The protocol can establish routes in a hybrid or a proactive mode and uses an adaptive continuous routing metric which makes it very flexible in terms of scalability while maintaining stable routes. The hybrid mode is optimized for low-power WSNs since routes are only established on demand. The difference of the hybrid mode to reactive routing strategies is that routing messages are periodically transmitted to maintain already established routes. However, the protocol stops the transmission of routing messages if no data packets are transmitted for a certain time period in order to minimize the routing overhead and the energy consumption. The proactive mode is designed for high data rate networks which have less energy constraints. In this mode, the protocol periodically transmits routing messages to establish routes in a proactive way even in the absence of data traffic. Thus, nodes in the network can immediately transmit data since the route to the destination is already established in advance. In addition, a new delay-based routing message forwarding strategy is introduced. The forwarding strategy is part of SBR but can also be applied to many routing protocols in order to modify the established topology. The strategy can be used, e.g. in mobile networks, to decrease the packet loss by deferring routing messages with respect to the neighbor change rate. Thus, nodes with a stable neighborhood forward messages faster than nodes within a fast changing neighborhood. As a result, routes are established through nodes with correlated movement which results in fewer topology changes due to higher link durations.},
  subject      = {Routing},
  language  = {en}
}