@article{NeumannOhlsenDonatetal.2015,
  author    = {Neumann, Yvonne and Ohlsen, Knut and Donat, Stefanie and Engelmann, Susanne and Kusch, Harald and Albrecht, Dirk and Cartron, Michael and Hurd, Alexander and Foster, Simon J.},
  title     = {The effect of skin fatty acids on Staphylococcus aureus},
  series = {Archives of Microbiology},
  volume    = {197},
  journal   = {Archives of Microbiology},
  doi       = {10.1007/s00203-014-1048-1},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-121657},
  pages     = {245-67},
  year      = {2015},
  abstract  = {Staphylococcus aureus is a commensal of the human nose and skin. Human skin fatty acids, in particular cis-6-hexadecenoic acid (C-6-H), have high antistaphylococcal activity and can inhibit virulence determinant production. Here, we show that sub-MIC levels of C-6-H result in induction of increased resistance. The mechanism(s) of C-6-H activity was investigated by combined transcriptome and proteome analyses. Proteome analysis demonstrated a pleiotropic effect of C-6-H on virulence determinant production. In response to C-6-H, transcriptomics revealed altered expression of over 500 genes, involved in many aspects of virulence and cellular physiology. The expression of toxins (hla, hlb, hlgBC) was reduced, whereas that of host defence evasion components (cap, sspAB, katA) was increased. In particular, members of the SaeRS regulon had highly reduced expression, and the use of specific mutants revealed that the effect on toxin production is likely mediated via SaeRS},
  language  = {en}
}
@article{SelleHertleinOesterreichetal.2016,
  author    = {Selle, Martina and Hertlein, Tobias and Oesterreich, Babett and Klemm, Theresa and Kloppot, Peggy and M{\"u}ller, Elke and Ehricht, Ralf and Stentzel, Sebastian and Br{\"o}ker, Barbara M. and Engelmann, Susanne and Ohlsen, Knut},
  title     = {Global antibody response to Staphylococcus aureus live-cell vaccination},
  series = {Scientific Reports},
  volume    = {6},
  journal   = {Scientific Reports},
  doi       = {10.1038/srep24754},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-181245},
  year      = {2016},
  abstract  = {The pathogen Staphylococcus aureus causes a broad range of severe diseases and is feared for its ability to rapidly develop resistance to antibiotic substances. The increasing number of highly resistant S. aureus infections has accelerated the search for alternative treatment options to close the widening gap in anti-S. aureus therapy. This study analyses the humoral immune response to vaccination of Balb/c mice with sublethal doses of live S. aureus. The elicited antibody pattern in the sera of intravenously and intramuscularly vaccinated mice was determined using of a recently developed protein array. We observed a specific antibody response against a broad set of S. aureus antigens which was stronger following i.v. than i.m. vaccination. Intravenous but not intramuscular vaccination protected mice against an intramuscular challenge infection with a high bacterial dose. Vaccine protection was correlated with the strength of the anti-S. aureus antibody response. This study identified novel vaccine candidates by using protein microarrays as an effective tool and showed that successful vaccination against S. aureus relies on the optimal route of administration.},
  language  = {en}
}