@article{GorboulevAkhundovaGrzeschiketal.1994, author = {Gorboulev, Valentin G. and Akhundova, Aida and Grzeschik, K.-H. and Fahrenholz, Falk}, title = {Organization and chromosomal localization of the gene for the human bombesin receptor subtype expressed in pregnant uterus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32572}, year = {1994}, abstract = {The gene encoding the human homologue of the guinea pig uterine bombesm receptor [( 1992) Eur. J. Biochem. 208,405] was isolated from a genomic lambda library by the PCR/homology screening approach. The gene spans more than 4 kb and consists of 3 exons and 2 introns. The deduced amino acid sequence shows about 86\% identity to that of guinea pig bombesin receptor. This subtype of bombesin receptor is expressed in the pregnant uterus and in two human tumour cell lines, T47D (ductal breast carcinoma) and A431 (epidermal carcinoma). PCR analysis of genomic DNA from human-mouse cell hybrids allows the cloned gene to be localized to the region q26q28 on chromosome X.}, language = {en} } @article{GorboulevBuechnerAkhundovaetal.1993, author = {Gorboulev, Valentin and B{\"u}chner, Hubert and Akhundova, Aida and Fahrenholz, Falk}, title = {Molecular cloning and functional characterization of V2 [8-Iysine] vasopressin and oxytocin receptors from a pig kidney cell line (LLC-PK1)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59311}, year = {1993}, abstract = {No abstract available}, subject = {Biologie}, language = {en} } @article{GorboulevAkhundovaLuziusetal.1992, author = {Gorboulev, Valentin and Akhundova, Aida and Luzius, Heike and Fahrenholz, Falk}, title = {Molecular cloning of substance P receptor cDNA from guinea-pig uterus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59293}, year = {1992}, abstract = {A cDNA encoding guinea-pig uterine substance P (SP) receptor has been isolated using the homology screening approach. Northern blot analysis reveals that the corresponding mRNA, of approx. 4.8 kb, is expressed in all tissues tested, but predominantly in the uteri of non-pregnant animals; during pregnancy its expression is reduced. The guinea-pig SP receptor was expressed in COS-7 cells and demonstrated relative Iigand affinity in the order: SP >> neurokinin A > neurokinin B.}, subject = {Biologie}, language = {en} } @article{GorboulevAkhundovaBuechneretal.1992, author = {Gorboulev, Valentin and Akhundova, Aida and B{\"u}chner, Hubert and Fahrenholz, Falk}, title = {Molecular cloning of a new bombesin receptor subtype expressed in uterus during pregnancy}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59304}, year = {1992}, abstract = {The homology screening approach has been used to clone a new member of the guanine-nucleotidebinding-protein-coupled receptor superfamily from guinea pig uterus. The cloned cDNA encodes a 399-amino-acid protein and shows the highest amino acid similarity to members of the bombesin receptor family; 52\% and 47\% similarity to the gastrin-releasing-peptide (GRP) receptor and the neuromedin-B receptor, respectively. Bindingexperiments with the stably transfected LLC-PK1 cell line expressing the new receptor protein confmned the bombesin-like nature of the cloned receptor. The relative order ofligand affinity, GRP = neuromedin C >> neuromedin B, suggests that the cloned cDNA represents the GRP subtype rather than the neuromedin-B subtype of bombesin receptors. Northern-blot analysis of mRNA species from several guinea-pig tissues showed that the mRNA for the new bombesin receptor subtype is expressed mainly in uteri of pregnant animals.}, subject = {Biologie}, language = {en} }