@article{SendtnerThoenen1994, author = {Sendtner, Michael and Thoenen, Hans}, title = {Oxidative stress and motorneuron disease}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42684}, year = {1994}, abstract = {Transgenic mice carrying mutated Cu/Zn superoxide dismutase genes provide insights into the pathogenesis of human motorneuron diseases and may be useful as models in the development and testing of therapies.}, language = {en} } @article{SendtnerThoenenHoltmannetal.1992, author = {Sendtner, Michael and Thoenen, Hans and Holtmann, B. and Kohlbeck, R. and Barde, Y.-A.}, title = {Brain-derived neurotrophic factor prevents the death of motoneurons in newborn rats after nerve section}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42673}, year = {1992}, abstract = {Motoneurons innervating the skeletal musculature were among the first neurons shown to require the presence of their target cells to develop appropriatelyl,2. But the characterization of molecules allowing motoneuron survival has been difficult. Ciliary neurotrophic factor prevents the death of motoneurons3-6, but its gene is not expressed during development7. Although the presence of a neurotrophin receptor on developing motoneurons8-1O has suggested a role for neurotrophins, none could be shown to promote motoneuron survival in vitro3. We report here that brainderived neurotrophic factor can prevent the death of axotomized motoneurons in newborn rats, suggesting a role for this neurotrophin for motoneuron survival in vivo.}, language = {en} } @article{HughesLillienRaffetal.1988, author = {Hughes, Simon M. and Lillien, Laura E. and Raff, Martin C. and Rohrer, Hermann and Sendtner, Michael}, title = {Ciliary neurotrophic factor induces type-2 astrocyte differentiation in culture}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42660}, year = {1988}, abstract = {We have been studying a population of bipotential glial progenitor cells in the perinatal rat optic nerve and brain in an attempt to understand how cells choose between alternative fates in the developing mammalian central nervous system (CNS). This cell population gives rise initially to oligodendrocytes and then to type-2 astrocytes1 both of which apparently collaborate in sheathing axons in the CNS2,3. In vitro studies suggest that oligodendrocyte differentiation is the constitutive pathway of development for the oligodendrocyte-type-2-astrocyte (O-2A) progenitor cell4,5, whereas type-2 astrocyte differentiation depends on a specific inducing protein6. This protein is present in the developing optic nerve when type-2 astrocytes are differentiating and can induce 0-2A progenitor cells in vitro to express glial fibrillary acidic protein (GFAP)6, a marker of astrocyte differentiation7. Here we show that the type-2-astrocyte-inducing protein is similar or identical to ciliary neutrotrophic factor (CNTF)8,9, which promotes the survival of some types of peripheral neurons in vitro8, including ciliary ganglion neurons8,10. This suggests that CNTF, in addition to its effect on neurons, may be responsible for triggering type-2 astrocyte differentiation in the developing CNS.}, language = {en} } @article{SendtnerThoenenHughes1993, author = {Sendtner, Michael and Thoenen, Hans and Hughes, R. A.}, title = {Members of several gene families influence survival of rat motoneurons in vitro and in vivo}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42652}, year = {1993}, abstract = {The survival and functional maintenance of spinal motoneurons, both during the period of developmental cell death and in adulthood, have been shown to be dependent on trophic factors. In vitro experiments have previously been used to identify several survival factors for motoneurons, including CNTF, UF, and members of the neurotrophin, FGF, and IGF gene families. Some of these factors have also been shown to be active in vivo, either on chick motoneurons during embryonic development or on lesioned facial and spinal motoneurons of the newborn rat. Here we demonstrate that lesioned newborn rat facial motoneurons can be rescued by NT-4/5, IGF-I, and UF. Furthermore, in contrast to chick motoneurons, the survival of isolated embryonic rat motoneurons can be maintained by the neurotrophins BDNF, NT-3, and NT-4/5. IGF-I and FGF-5 were also active in this system, each supporting more than 50\% of the originally plated neurons. The responsiveness of motoneurons to multiple factors in vitro and in vivo suggests that motoneuron survival and function are regulated by the coordinated actions of members of different gene families.}, language = {en} } @article{BenaventeReimerRoseetal.1988, author = {Benavente, Ricardo and Reimer, Georg and Rose, Kathleen M. and H{\"u}gle-D{\"o}rr, Barbara and Scheer, Ulrich}, title = {Nucleolar changes after microinjection of antibodies to RNA polymerase I into the nucleus of mammalian cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40666}, year = {1988}, abstract = {After microinjection of antibodies against RNA polymerase I into the nuclei of cultured rat kangaroo (PtKz) and rat (RVF-SMC) cells alterations in nucleolar structure and composition were observed. These were detected by electron microscopy and double-label immunofluorescence microscopy using antibodies to proteins representative of the three major components of the nucleolus. The microinjected antibodies produced a progressive loss of the material of the dense fibrillar component (DFC) from the nucleoli which, at 4 h after injection, were transformed into bodies with purely granular component (GC) structure with attached fibrillar centers (FCs). Concomitantly, numerous extranucleolar aggregates appeared in the nucleoplasm which morphologically resembled fragments of the DFC and contained a protein (fibrillarin) diagnostic for this nucleolar structure. These observations indicate that the topological distribution of the material constituting the DFC can be experimentally influenced in interphase cells, apparently by modulating the transcriptional activity of the rRNA genes. These effects are different from nucleolar lesions induced by inhibitory drugs such as actinomycin D-dependent "nucleolar segregation". The structural alterations induced by antibodies to RNA polymerase I resemble, however, the initial events of nucleolar disintegration during mitotic prophase.}, language = {en} } @article{BarresSchmidSendtneretal.1993, author = {Barres, B. A. and Schmid, R. and Sendtner, Michael and Raff, Martin C.}, title = {Multiple extracellular signals are required for long-term oligodendrocyte survival}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42644}, year = {1993}, abstract = {We showed previously that oligodendrocytes and their precursors require continuous signalling by protein trophic factors to avoid programmed cell death in culture. Here we show that three classes of such trophic factors promote oligodendrocyte survival in vitro: (1) insulin and insulin-like growth factors (IGFs), (2) neurotrophins, particularly neurotrophin-3 (NT -3), and (3) ciliary-neurotrophic factor (CNTF), leukemia inhibitory factor (LIF) and interleukin 6 (IL-6). A single factor, or combinations of factors within the same class, promote only short-term survival of oligodendrocytes and their precursors, while combinations of factors from different classes promote survival additively. Long-term survival of oligodendrocytes in vitro requires at least one factor from each class, suggesting that multiple signals may be required for long-term oligodendrocyte survival in vivo. We also show that CNTF promotes oligodendrocyte survival in vivo, that platelet-derived growth factor (PDGF) can promote the survival of oligodendrocyte precursors in vitro by acting on a novel, very high affinity PDGF receptor, and that, in addition to its effect on survival, NT-3 is a potent mitogen for oligodendrocyte precursor cells.}, language = {en} } @article{DittrichThoenenSendtner1994, author = {Dittrich, Falk and Thoenen, Hans and Sendtner, Michael}, title = {Ciliary neurotrophic factor: pharmacokinetics and acute-phase response in rat}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42639}, year = {1994}, abstract = {No abstract available}, language = {en} } @article{KaupmannSendtnerStoecklietal.1991, author = {Kaupmann, Klemens and Sendtner, Michael and St{\"o}ckli, Kurt A. and Jockusch, Harald}, title = {The gene of ciliary neurotrophic factor (cntf) maps to murine chromosome 19 and its expression is not affected in the hereditary motoneuron disease 'wobbler' of the mouse}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42626}, year = {1991}, abstract = {The cDNA for ciliary neurotrophic factor (CNTF), a polypeptide involved in the survival of motoneurons in mammals, has recently been cloned (St{\"o}ckli et al., Nature, 342, 920 - 923, 1989; Lin et al. Science, 246, 1023 - 1025, 1989). We have now localized the corresponding gene Cntf to chromosome 19 in the mouse, using an interspecific cross between Mus spretus and Mus musculus domesticus. The latter was carrying the gene wobbler (wr) for spinal muscular atrophy. DNA was prepared from backcross individuals and typed for the segregation of species-specific Cntf restriction fragments in relation to DNA markers of known chromosomal location. The M.spretus allele of Cntf cosegregated with chromosome 19 markers and mapped closely to Ly-1, to a region of mouse chromosome 19 with conserved synteny to human chromosome 11q. Cntf is not linked to wr, and the expression of CNTF mRNA and protein appears close to normal in facial and sciatic nerves, of affected (wr/wr) mice, suggesting that motoneuron degeneration of wobbler mice has its origin in defects other than reduced CNTF expression.}, language = {en} } @article{SendtnerGnahnWakadeetal.1988, author = {Sendtner, Michael and Gnahn, H. and Wakade, A. and Thoenen, Hans}, title = {Is activation of the Na\(^+\)K\(^+\) pump necessary for NGF-mediated neuronal survival?}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42610}, year = {1988}, abstract = {The ability of nerve growth factor to cause rapid activation of the Na+K+ pump of its responsive cells was examined by measuring the uptake of 86Rb+. A significant increase in 86Rb+ uptake in Ea chick dorsal root ganglion sensory neurons after NGF treatment was seen only if the cells had been damaged during the preparation procedure. Such damaged cells could not survive in culture in the presence of NGF, and undamaged cells that did survive in response to NGF exhibited no increased 86Rb+ uptake rate. Furthermore, cultured calf adrenal medullary cells did not show an increase in 86Rb+ uptake after treatment with NGF, although these cells respond to NGF with an increased synthesis of catecholaminergic enzymes. These results are incompatible with the hypothesis that the mechanism of action of NGF that promotes neuronal survival and enzyme induction results from an initial stimulation of the Na+K+ pump.}, language = {en} } @article{LillienSendtnerRaff1990, author = {Lillien, Laura E. and Sendtner, Michael and Raff, Martin C.}, title = {Extracellular Matrix-associated molecules collaborate with ciliary neurotrophic factor to induce type-2 astrocyte development}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42602}, year = {1990}, abstract = {0-2A progenitor cells give rise to both oligodendrocytes and type-2 astrocytes in vitro. Whereas oligodendrocyte differentiation occurs constitutively, type-2 astrocyte differentiation requires extracellular signals, one of which is thought to be ciliary neurotrophic factor (CNTF). CNTF, however, is insufficient by itself to induce the development of stable type-2 astrocytes. In this report we show the following: (a) that molecules associated with the extracellular matrix (ECM) cooperate with CNTF to induce stable type-2 astrocyte differentiation in serumfree cultures. The combination of CNTF and the ECM-associated molecules thus mimics the effect of FCS, which has been shown previously to induce stable type-2 astrocyte differentiation in vitro. (b) Both the ECM-associated molecules and CNTF act directly on 0-2A progenitor cells and can induce them to differentiate prematurely into type-2 astrocytes. (c) ECM-associated molecules also inhibit oligodendrocyte differentiation, even in the absence of CNTF, but this inhibition is not sufficient on its own to induce type-2 astrocyte differentiation. (d) Whereas the effect of ECM on oligodendrocyte differentiation is mimicked by basic fibroblast growth factor (bFGF), the effect of ECM on type-2 astrocyte differentiation is not. (e) The ECM-associated molecules that are responsible for inhibitin~ oligodendrocyte differentiation and for cooperating with CNTF to induce type-2 astrocyte differentiation are made by non-glial cells in vitro. (f) Molecules that have these activities and bind to ECM are present in the optic nerve at the time type-2 astrocytes are thought to be developing.}, language = {en} } @article{SendtnerStoeckliCarrolletal.1992, author = {Sendtner, Michael and St{\"o}ckli, Kurt A. and Carroll, Patrick and Kreutzberg, Georg W. and Thoenen, Hans}, title = {More on motor neurons}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42598}, year = {1992}, abstract = {No abstract available}, language = {en} } @article{FrankeScheerTrendelenburgetal.1976, author = {Franke, Werner W. and Scheer, Ulrich and Trendelenburg, Michael F. and Spring, Herbert and Zentgraf, Hanswalter}, title = {Absence of nucleosomes in transcriptionally active chromatin}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40646}, year = {1976}, abstract = {The ultrastructure of twO kinds of transcription ally active chromatin, the lampbrush chromosome loops and the nucleoli from amphibian oocytes and primary nuclei of the green alga Acetabularia, has been examined after manual isolation and dispersion in low salt media of slightly alkaline pH using various electron microscopic staining techniques (positive staining, metal shadowing, negative staining, preparation on positively charged films, etc.) and compared with the appearance of chromatin from various somatic cells (hen erythrocytes, rat hepatocytes, ClIltured murine sarcoma cells) prepared in parallel. While typical nucleosomes were revealed with all the techniques for chromatin from the latter three cell system, no nucleosomes were identified in either the lampbrush chromosome structures or the nucleolar chromatin. Nucleosomal arrays were absent not only in maximally fibril-covered matrix units but also in fibril-free regions between transcriptional complexes, including the apparent spacer intercepts between different transcriptional units. Moreover, comparisons of the length of the repeating units of rDNA in the transcribed state with those determined in the isolated rDNA and with the lengths of the first stable product of rDNA transcription, the pre-rRNA, demonstrated that the transcribed rDNA was not significantly shortened and/or condensed but rather extended in the transcriptional units. Distinct granules of about nucleosomal size which were sometimes found in apparent spacer regions as well as within matrix units of reduced fibril density were shown not to represent nucleosomes since their number per spacer unit was not inversely correlated with the length of the specific unit and also on the basis of their resistance to treatment with the detergent Sarkosyl NL-30. It is possible to structurally distinguish between transcriptionally active chromatin in which the DNA is extended in a non-nucleosomal form of chromatin and condensed, inactive chromatin within the typical nucleosomal package. The characteristic extended structure of transcriptionally active chromatin is found not only in the transcribed genes but also in non-transcribed regions within or between ("spacer") transcriptional units as well as in transcriptional units that are untranscribed amidst transcribed ones and/or have been inactivated for relatively short time. It is hypothesized that activation of transcription involves a transition from a nucleosomal to an extended chromatin organisation and that this structural transition is not specific for single "activated" genes but may involve larger chromatin regions, including adjacent untranscribed intercepts.}, subject = {Cytologie}, language = {en} } @article{EckertFrankeScheer1972, author = {Eckert, W. A. and Franke, Werner W. and Scheer, Ulrich}, title = {Actinomycin D and the central granules in the nuclear pore complex: thin sectioning versus negative staining}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40636}, year = {1972}, abstract = {Thin section electron microscopy of Actinomycin D treated Tetrahymena cells and amphibian oocytes (Xenopus laevis, Triturus aZpestris) reveal no reduction in the central granules in the nuclear pore complexes. Possible reasons for the diversity between these results and earlier observations using negatively stained isolated nuclear envelopes from the same objects are discussed. The results clearly show that the presence of central granules within the nuclear pores does neither depend on nuclear RNA synthesis nor does indicate nucleocytoplasmic RNA transport. This conclusion leads to a reconsideration of the nature of the central granule. The functioning of the central granule of the nuclear pore complexes is further discussed in connection with recent studies on the ultrastructure of various types of cisternal pores.}, language = {en} } @article{FrankeScheer1971, author = {Franke, Werner W. and Scheer, Ulrich}, title = {Some structural differentiations in the HeLa cell: heavy bodies, annulate lamellae and cotte de maillet endoplasmic reticulum}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40614}, year = {1971}, abstract = {A small fraction of HeLa cells within an exponentially growing culture showed cisternal differentiations, such as cytoplasmic as well as intranuclear annulate lamellae and special smooth surfaced endoplasmic reticulum aggregates with a typical "Cotte de maillet" appearance. Additionally, clusters of dense granules were observed in the cytoplasm which were often associated with polysomes and strongly resembled the so-called "heavy bodies" known in particular in diverse oocytes. The functional meaning of these structures is discussed. Moreover, it is deduced from the ultrastructural identity of the pore complexes in the nuclear envelope and the cytoplasmic and intranuclear annulate lamellae that the pore complex material with its highly ordered arrangement is not a structure characteristic for nucleocytoplasmically migrating material, but rather is a general structural expression of a tight binding of ribonucleoprotein (RNP) to cisternal membranes. The pore complexes are thought of as representing sites of a RNP-storage. A similar functioning is hypothesized for the "heavy body"like aggregates. To the current hypotheses on the formation of annulate lamellae and the nuclear envelope, which are based on the concept of membrane continuities and constancies, the alternative view of a self assembly mechanism of membrane constituents on nucleoprotein structures is added.}, subject = {Cytologie}, language = {en} } @article{HughesSendtnerGoldfarbetal.1993, author = {Hughes, Richard A. and Sendtner, Michael and Goldfarb, Mitchell and Lindholm, Dan and Thoenen, Hans}, title = {Evidence that fibroblast growth factor 5 is a major muscle-derived survival factor for cultured spinal motoneurons}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42588}, year = {1993}, abstract = {We examined the potential role of fibroblast growth factor 5 (FGF-5) as a target-derived trophic factor for spinal motoneurons. Northern analysis of total RNA from rat skeletal muscle revealed an FGF-5 mRNA transcript both during the period of embryonic motoneuron death and in the adult. Recombinant human FGF-5 supported the survival of highly enriched cultures of embryonic chick motoneurons. Significant proportions of the motoneuron survival activity of rat skeletal muscle extracts could be immunoprecipitated using an antiserum to FGF-5. The immunoprecipitable activity was present in soluble and matrix-bound forms in embryonic muscle, but bound exclusively to the extracellular matrix in adult muscle. These results, along with the secretory nature of FGF-5, suggest that FGF-5 may act as a target-derived trophic factor for motoneurons.}, language = {en} } @article{SendtnerStoeckliThoenenetal.1992, author = {Sendtner, Michael and St{\"o}ckli, Kurt A. and Thoenen, Hans and Schmalbruch, H. and Carroll, P. and Kreutzberg, Georg W.}, title = {Ciliary neurotrophic factor prevents the degeneration of motor neurons in mouse mutant progressive motor neuronopathy}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42563}, year = {1992}, abstract = {CILIARY neurotrophic factor (CNTF) supports the survival of embryonic motor neurons in vitro and in vivo and prevents lesion-mediated degeneration of rat motor neuron~ during early post-natal stages. Here we report that CNTF greatly reduces all the functional and morphological changes in pmnlpmn mice5, an autosomal recessive mutant leading to progressive caudo-cranial motor neuron degeneration. The first manifestations of progressive motor neuronopathy in homozygous pmnl pmn mice become apparent in the hind limbs at the end of the third post-natal week and all the mice die up to 6 or 7 weeks after birth from respiratory paralysis. Treatment with CNTF prolongs- survival- and greatly Impoves motor function of these mice. Moreover, morphological manifestations, such as loss of motor axons in the phrenic nerve and degeneration of facial motor neurons, were greatly reduced by CNTF, although the treatment did not start until the first symptoms of the disease had already become apparent and substantial degenerative changes were already present. The protective and restorative effects of CNTF in this mouse mutant give new perspectives for the treatment of human degenerative motor neuron diseases with CNTF.}, language = {en} } @article{SpringTrendelenbrugScheeretal.1974, author = {Spring, Herbert and Trendelenbrug, Michael F. and Scheer, Ulrich and Franke, Werner W. and Herth, Werner}, title = {Structural and biochemical studies of the primary nucleus of two green algal species, Acetabularia mediterranea and Acetabularia major}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40600}, year = {1974}, abstract = {Primary (giant) nuclei of the green algae Acetabularia mediterranea and A. major were studied by light and electron microscopy using in situ fixed material as well as manually isolated nuclear components. In addition, cytochemical reactions of nuclear structures and biochemical determinations of nuclear and cytoplasmic RNA and of genome DNA content were performed. The data obtained and the structures observed are interpreted as demonstralions of transcriptional activities of different gene classes. The most prominent class is the nucleolar cistrons of precursors of ribosomal RNA which occur highly repeated in clusters in the form of regularly alternating intercepts on deoxyribonucleoprotein axes of transcribed rDNA, the fibril-covered matrix units, and the fibril-free "spacer" segments. A description and a classification of the various structural complexes which seem to represent transcriptional activities is given. Quantitative evaluations of these arrangements are presented. The morphology and the dimensions of such structures are compared with the RNA molecular weight determinations and with the corresponding data reported from various animal cell systems. It is suggested that the formation of the giant nucleus is correlated with, and probably due to, an enormous amplification of transcriptionally active rDNA and packing of the extrachromosomal copies into the large nucleolar aggregate bodies.}, subject = {Cytologie}, language = {en} } @article{SendtnerCarrollHoltmannetal.1994, author = {Sendtner, Michael and Carroll, P. and Holtmann, B and Hughes, R. A. and Thoenen, H.}, title = {Ciliary Neurotrophic Factor}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42545}, year = {1994}, abstract = {No abstract available}, language = {en} } @article{FrankeZentgrafScheer1973, author = {Franke, Werner W. and Zentgraf, Hanswalter and Scheer, Ulrich}, title = {Membrane linkages at the nuclear envelope}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40596}, year = {1973}, abstract = {Electron-opaque material is shown in the perinuclear cisternae of various cell types to connect the inner and outer nuclear membrane faces. Similar bridges were observed between the outer nuclear membrane and the outer mitochondrial membrane. The intracisternal bridges of the nuclear envelope appear to be important for the structural stability of the perinuclear cisterna. Stable structural linkage of mitochondria to the outer nuclear membrane might be relevant to the understanding of the characteristic juxtanuclear accumulation of mitochondria and also provide arguments for the discussions of certain biochemical activities found in nuclear and nuclear membrane fractions.}, subject = {Cytologie}, language = {en} } @article{FrankeScheerZentgraf1984, author = {Franke, Werner W. and Scheer, Ulrich and Zentgraf, Hanswalter}, title = {Organization of transcriptionally active and inactive chromatin}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40588}, year = {1984}, abstract = {No abstract available}, subject = {Deutschland}, language = {en} } @article{WeisenbergerScheerBenavente1993, author = {Weisenberger, Dieter and Scheer, Ulrich and Benavente, Ricardo}, title = {The DNA topoisomerase I inhibitor camptothecin blocks postmitotic reformation of nucleoli in mammmalian cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-41434}, year = {1993}, abstract = {No abstract available}, subject = {Cytologie}, language = {en} } @article{SaadatSendtnerRohrer1989, author = {Saadat, S. and Sendtner, Michael and Rohrer, H.}, title = {Ciliary neurotrophic factor induces cholinergic differentiation of rat sympathetic neurons in culture}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32677}, year = {1989}, abstract = {Ciliary neurotrophic factor (CNTF) influences the levels of choline acetyltransferase (ChAT) and tyrosine hydroxylase (TH) in cultures of dissociated sYmpathetic neurons from newborn rats. In the presence of CNTF both the total and specific activity of ChAT was increased 7 d after culture by 15- and 18-fold, respectively, as compared to cultures kept in the absence of CNTF. Between 3 and 21 d in culture in the presence of CNTF . the total ChAT activity increased by a factor of >100. Immunotitration demonstrated that the elevated ChAT levels were due to an increased number of enzyme molecules. In contrast to the increase in ChAT levels, the total and specific activity levels' of TH were decreased by 42 and 36 \%, respectively, after 7 d in culture. Half-maximal effects for both ChAT increase and TH decrease were obtained at CNTF concentrations of rvO.6 ng and maximal levels were reached at I ng of CNTF per milliliter of medium. The effect of CNTF on TH and ChAT levels were seen in serum-containing medium as well as in serum-free medium. CNTF was shown to have only a small effect on the long-term s.urviVal of rat sympathetic neurons. We therefore concluded that the effects of CNTF on ChAT and TH are not due to selective survival of cells that acquire cholinergic traits in vitro, but are rather due to the induction of cholinergic differentiation of noradrenergic sympathetic neurons.}, language = {en} } @article{FialaMaschwitz1991, author = {Fiala, Brigitte and Maschwitz, Ulrich}, title = {Extrafloral nectaries in the genus Macaranga (Euphorbiaceae) in Malaysia: comparative studies of their possible significance as predispositions for myrmecophytism.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42863}, year = {1991}, abstract = {So me species of the paleotropical tree genus Macaranga (Euphorbiaceae) live in elose association with ants. Thc genus comprises the full range of species from those not regularly inhabited by ants to obligate myrmecophytes. In Malaysia (peninsular and Borneo) 23 ofthe 52 species areknown to be ant-associated (44\%). The simplest structural adaptation of plants to attract ants are extrafloral nectaries. We studied the distribution of extraflural nectaries in the genus Macaranga to assess the significance of this character as a possible predisposition for the evolution of obligate myrmecophytism. All species have marginal glands on the leaves. However, only the glands of nonmyrmecophytic species function as nectaries, whereas liquids secreted by these glands in myrmecophytic species did not contain sugar. Some non-myrmecophytic Macaranga and transitional Macaranga species in addition have extrafloral nectaries on the leaf blade near the petiole insertion. All obligatorily myrmecophytic Macaranga species, however, lack additional glands on the lamina. The non-myrmecophytic species are visited by a variety of different ant species, whereas myrmecophytic Macaranga are associated only with one specific ant-partner. Since these ants keep scale insects in the hollow sterns, reduction of nectary production in ant-inhabited Macaranga seems to be biologically significant. We interpret this as a means of (a) saving the assimilates and (b) stabilization of maintenance of the association's specificity. Competition with other ant species for food rewards is avoided and thereby danger ofweakening the protective function ofthe obligate antpartner for the plant is reduced. A comparison with other euphorb species living in the same habitats as Macaranga showed that in genera in which extrafloral nectaries are widespread, no myrmecophytes have evolved. Possession of extrafloral nectaries does not appear to be essential for the development of symbiotic ant-plant interactions. Other predispositions such as nesting space might have played a more important role.}, subject = {Macaranga}, language = {en} } @article{MaschwitzFialaLinsenmair1992, author = {Maschwitz, Ulrich and Fiala, Brigitte and Linsenmair, K. Eduard}, title = {A new ant-tree from SE Asia: Zanthoxylum myriacanthum (Rutaceae), the Thorny Ivy-Rue}, isbn = {0025-1291}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42967}, year = {1992}, abstract = {Zanthoxylum myriacanthum, a small Rutaceous tree growing mainly in secondary hill forests in SE Asia, is a true myrmecophyte. It possesses stem domatia in the form of hollow branches with slitlike openings. Branch hollows and entrance slits are produced by the plant itself through pith degene~.tion ?u.d growth proceSses. If the entrance is not kept open by ants it closes again by growth ol the surrounding tissue after some time. The domatia are colonized opportunistic ally by different arboreous ants, e.g. Crematogaster and Campono tus. Additionally many small extrafloral nectaries are found on the leaflets of Zanthoxylum myriacanthum. Judging from herbarium studies and literature records at least four more true ant trees are found in the genus Zanthoxylum namely Z. rhetsa in SE Asia, Z. conspersipunctatum, Z. pluviatile and Z. vinkii in New Guinea. We could not confirm ant inhabitation in Drypetes pendula (Euphorbiaceae) on the Malay Peninsula, which has also been recorded to be an anttree.}, language = {en} } @article{HughesMuellerHackeretal.1982, author = {Hughes, Colin and M{\"u}ller, Dorothee and Hacker, J{\"o}rg and Goebel, Werner}, title = {Genetics and pathogenic role of Escherichia coli hemolysin}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40082}, year = {1982}, abstract = {While clear evidence exists for the direct involvement of cytolysins in the pathogenesis of Gram-positive bacteria, the significance of Gram-negative haemolysins remains unclear. This paper presents briefly data indicating a role for haemolysin production in infections caused by Escherichia coli and also experiments which have allowed an analysis of the molecular basis of the haemolysis among pathogenic and non-pathogenic strains of this species.}, language = {en} } @article{KlemdMatthesSchuessler1994, author = {Klemd, R. and Matthes, S. and Sch{\"u}ssler, Ulrich}, title = {Reaction textures and fluid behaviour in very high-pressure calc-silicate rocks of the M{\"u}nchberg gneiss complex, Bavaria, Germany}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31035}, year = {1994}, abstract = {Calc-silicate rocks occur as elliptical bands and boudins intimately interlayered with eclogites and high-pressure gneisses in the Munchberg gneiss complex of NE Bavaria. Core assemblages of the boudins consist of grossular-rich garnet, diopside, quartz, zoisite, clinozoisite, calcite, rutile and titanite. The polygonal granoblastic texture commonly displays mineral relics and reaction textures such as postkinematic grossular-rich garnet coronas. Reactions between these mineral phases have been modelled in the CaO-Al203-Si02-C02-H2 0 system with an internally consistent thermodynamic data base. High-pressure metamorphism in the calc-silicate rocks has been estimated at a minimum pressure of 31 kbar at a temperature of 630°C with X^oSQ.Gi. Small volumes of a C02-N2-rich fluid whose composition was buffered on a local scale were present at peak-metamorphic conditions. The P-T conditions for the onset of the amphibolite facies overprint are about 10 kbar at the same temperature. A'co., of the H20-rich fluid phase is regarded to have been <0.03 during amphibolite facies conditions. These P-T estimates are interpreted as representing different stages of recrystallization during isothermal decompression. The presence of multiple generations of mineral phases and the preservation of very high-pressure relics in single thin sections preclude pervasive post-peak metamorphic fluid flow as a cause of a re-equilibration within the calc-silicates. The preservation of eclogite facies, very high-pressure relics as well as amphibolite facies reactions textures in the presence of a fluid phase is in agreement with fast, tectonically driven unroofing of these rocks.}, language = {en} } @article{FialaMaschwitz1992, author = {Fiala, Brigitte and Maschwitz, Ulrich}, title = {Food bodies and their significance for obligate ant-association in the tree genus Macaranga (Euphorbiaceae)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32921}, year = {1992}, abstract = {The production of extrafloral nectar and food bodies plays an important role in many tropical ant-plant mutualisms. In Malaysia, a close association exists between ants and some species of the pioneer tree genus Macaranga (Euphorbiaccac). Macaranga is a very diverse genus which exhibits all stages ofintcraction with ants, from facultative to obligatory associations. The ants nest inside the hollow inlcrnodes and reed mainly on food budies provided by the plants. Food body production had previously been reported only in myrrnecophytic Macaranga species, where it is usually coneentrated on protected parts or the plants such as recurved stipules. We found that non-myrmecophytic Macaranga species also produce food bodies on leaves and stems, where they are collected by a variety or ants. Levels of food body production differ between facultatively and obligatorily ant-associated species but also among the various non-myrmecophytes. This may he rdated to the degree of interaction with ants. Food body production starts at a younger age in the myrmccophytic species than in the transitional or non-myrmcccophytic Macaranga. Although food bodies of the non-inhabited Macaranga species are collected by a variety of ants, there is nu evidence of association with specific ant species. Our observations suggest that food bodies enhance the evolution of ant-plant interactions. Production of food bodies alone, however, does not appear to be the most important factor for the development of obligate myrmccopllytism in Macaranga.}, language = {en} } @article{GreinerPolignoneBeckeretal.1992, author = {Greiner, P. and Polignone, L. and Becker, Charles R. and Geick, R.}, title = {Contactless measurement of the conductivity of II-VI epitaxial layers by means of the partially filled waveguide method}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37838}, year = {1992}, abstract = {We report the contactless determination of the conductivity, the mobility and the carrier concentration of II-VI semiconductors by means of the technique of the partially filled waveguide at a microwave frequency of 9 GHz. The samples are CdHgTe epitaxial layers, grown on CdZnTe substrates by molecular beam epitaxy. The conductivity is determined from the transmission coefficient of the sample in the partially filled waveguide. For the analysis of the experimental data, the complex transmission coefficient is calculated by a rigorous multi-mode matching procedure. By varying the conductivity of the sample, we obtain an optimum fit of the calculated data to the experimental results. Comparison with conductivity data determined by the van der Pauw method shows that our method allows to measure the conductivity with good accuracy. The behaviour of the transmission coefficient of the sample is discussed in dependence on the layer conductivity, the layer thickness and the dielectric constant of the substrate. The calculations require to consider in detail the distribution of the electromagnetic fields in the sample region. The usual assumption of a hardly disturbed TE\(_{10}\) mode cannot be used in our case. By applying a magnetic field in extraordinary Voigt configuration. galvanomagnetic measurements have been carried out which yield the mobility and thus the carrier concentration. These results are also in good agreement with van der Pauw transport measurements.}, language = {en} } @article{KuninHuaVanArsdaleWhiteetal.1993, author = {Kunin, Calvin M. and Hua, Tong Hua and Van Arsdale-White, Laura and Krishnan, Chandradekar and Hacker, J{\"o}rg}, title = {Isolation of a nicotinamide-requiring clone of Escherichia coli O18:K1:H7 from women with acute cystitis resembles strains found in neonatal meningitis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40406}, year = {1993}, abstract = {During a study of the nutritional requirements of clinical isolates of Escherichia coli, we found that 21 (7.0\%) of 301 strains required nicotinamide to grow in minimal medium. The nicotinamide- requiring strains were present in 16 (15.8\%) of 101 cultures of urine from young women with acute cystitis, in 5 (5.0\%) of 100 stool specimens from healthy adults, and in none of 100 blood samples from adult patients with bacteremia. Most of the strains belonged to serogroup OI8:KI:H7, were hemolytic, possessed type I fimbriae, and exhibited similar patterns of antibiotic susceptibility. Two of the urinary isolates expressed S fimbriae, and all 16 urinary isolates contained the s/aS homologue gene on their chromosomes. One of the stool isolates contained the s/aS gene. The urinary isolates closely resembled a large clone of E. coli that is reportedly associated with neonatal meningitis and sepsis. It may be possible to detect this and related clones by their requirement for nicotinamide and to screen strains for S fimbriae by relatively inexpensive hemagglutination methods, including the use of avian PI antigens to detect mannose- resistant, non-P-fimbriated E. coli; the agglutination of bovine erythrocytes; and the use of bovine mucin to detect sialyl galactosides in S fimbriae.}, language = {en} } @article{LueckBenderOttetal.1991, author = {L{\"u}ck, P. Christian and Bender, Larisa and Ott, Manfred and Helbig, J{\"u}rgen H. and Hacker, J{\"o}rg}, title = {Analysis of Legionella pneumophila serogroup 6 strains isolated from a hospital warm water supply over a three-year period by using genomic long-range mapping techniques and monoclonal antibodies}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40392}, year = {1991}, abstract = {Over a period of 3 years, Legionella pneumophila serogroup 6 strains were isolated from warm water outlets and dental units in the Dental Faculty and from the Surgery and Internal Medicine Clinics at the University of Dresden, Dresden, Germany. In the bacteriological unit of the above-mentioned facility, L. pneumophila serogroups 3 and 12 were grown frl,)m warm water specimens. The medical facilities are located in separate buildings connected with a ring pipe warm water system. All L. pneumophila serogroup 6 strains isolated from the warm water supply reacted with a serogroup-specific monoclonal antibody, but not with two other monoclonal antibodies which are subgroup specific, reacting with other serogroup 6 strains. The NolI genomic profiles obtained by pulsed-field gel electrophoresis of 25 serogroup 6 strains isolated from the Dental Faculty over a 3-year period, 1 isolate from the Internal Medicine Clinic, and 4 strains from the Surgery Clinic were identical. Furthermore, all these strains hybridized with a 3OO-kb NolI fragment when a legiolysin (lIy)-specific DNA probe was used. The NolI pattern, however, differed from those of six serogroup 6 strains of other origins, one serogroup 12 strain from the bacteriological unit, and another six unrelated strains of serogroups other than serogroup 6. L. pneumophila serogroup 6 strains which can be divided into only two subgroups by the use of monoclonal antibodies are differentiated in at least six Noli cleavage types obtained by pulsed-field electrophoresis.}, language = {en} } @article{VanDieKramerHackeretal.1991, author = {Van Die, I. and Kramer, C. and Hacker, J{\"o}rg and Bergmans, H. and Jongen, W. and Hoekstra, W.}, title = {Nucleotide sequence of the genes coding for minor fimbrial subunits of the F1C fimbriae of Escherichia coli}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40353}, year = {1991}, abstract = {F 1 C fimbriae allow uropathogenic Escherichia coli to adhere to specific epithelial surfaces. This adhesive property is probably due to the presence of minor fimbrial components in F1C fimbriae. The foe gene cluster encoding F1C fimbriae has been cloned, as described previously. Here we present the nucleotide sequence (2081 bp) coding for the F 1 C minor fimbria I subunits. The structural genes code for polypeptides of 175 (FocF), 166 (FocG), and 300 (FocH) amino acids. The deduced amino acids of the F 1 C minor subunits were compared with the reported sequences of the minor subunits of other types of fimbriae. The data show that the Foc minor subunits are highly homologous to the corresponding Sfa proteins, whereas homology to the minor subunits of type 1 and P fimbriae is much lower.}, language = {en} } @article{ChakrabortyKathariouHackeretal.1987, author = {Chakraborty, Trinad and Kathariou, Sophia and Hacker, J{\"o}rg and Hof, Herbert and Huhle, Burkhard and Wagner, Wilma and Kuhn, Michael and Goebel, Werner}, title = {Molecular analysis of bacterial cytolysins}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40328}, year = {1987}, abstract = {Results of molecular and pathogenic studies of three different bacterial hemolysins (cytolysins) are presented. These exoproteins derive from the two gram-negative bacteria Escherichia coli and Aeromonas hydrophila and from the gram-positive pathogen Listeria monocytogenes. The hemolysin of E. coli is determined by an 8-kilobase (kb) region that includes four clustered genes (hlyC, hlyA, hlyB, and hlyD). This hemolysin determinant is part either of large transmissible plasmids or of the chromosome. The genes located chromosomally are found predominantly in E. coli strains that can cause pyelonephritis and/or other extraintestinal infections. A detailed analysis of the chromosomal hly determinants of one nephropathogenic E. coli strain revealed the existence of specific, large chromosomal insertions 75 kb and lOO kb in size that carry the hly genes but that also influence the expression of other virulence properties, i.e., adhesion and serum resistance. The direct involvement of E. coli hemolysin in virulence could be demonstrated in several model systems. The genetic determinants for hemolysin (cytolysin) formation in , A. hydrophila (aerolysin) and L. monocytogenes (listeriolysin) are less complex. Both cytolysins seem to be encoded by single genes, although two loci (aerB and aerC) that affect the expression and activity of aerolysin have been identified distal and proximal to the structural gene for aerolysin (aerA). Cytolysin-negative mutants of both bacteria were obtained by site-specific deletion and/or transposon mutagenesis. These mutants show a drastic reduction in the virulence of the respective bacteria.}, language = {en} } @article{HofChristenHacker1986, author = {Hof, H. and Christen, A. and Hacker, J{\"o}rg}, title = {Comparative therapeutic activities of Ciprofloxacin, Amoxicillin, Ceftriaxone and Cotrimoxazole in a new model of experimental infection with Escherichia coli}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40313}, year = {1986}, abstract = {A new mouse model for systemic infection with Escherichia coli is presented. Whereas in other models 107_108 bacteria have to be injected into an animal to induce toxic effects resulting in death within 24 hours, now, only 103_104 bacteria of an appropriate strain are required to produce a genuine infection characterized by an increase in the bacterial load over several days. The quantitative determination of bacterial counts per liver allows a more sensitive measurement than recording death rates. Furthermore, few animals are required for a definite result in contrast to the LDso determination of other models. The salient point regarding this new model is that conditioning of animals has to be achieved by incorporating the inoculum into agar which is injected subcutaneously. The resulting infection is completely dependent on the E. colicondistrain used. Whereas a hemolytic, uropathogenic strain is so virulent that an overwhelming infection develops within 48 hours after the injection of 103 bacterial cells, a non-hemolytic variant of this strain is completely avirulent, being unable to multiply in spite of the potentiating agar. The hemolytic E. coli strain ATCC 25922 is intermediate in virulence. The bacterial counts per liver increase steadily until death occurs five to seven days after the injection of 104 bacteria. This bacterial infection can be therapeutically influenced by daily treatment with various drugs. Ciprofloxacin, ceftriaxone and co-trimoxazole are able to cure the infection, whereas amoxicillin given orally is only moderately active against this ATCC strain, which is relatively resistant to amoxicillin.}, language = {en} } @article{HackerHofHughesetal.1985, author = {Hacker, J{\"o}rg and Hof, H. and Hughes, C. and Goebel, W.}, title = {Salmonella typhimurium strains carrying hemolysin plasmids and cloned hemolysin. genes from Escherichia coli}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40309}, year = {1985}, abstract = {Like all other Salmonella typhimurium strains examined, the smooth variants SF1397 (L T2) and 1366 and also their semi-rough and rough derivatives are non-haemolytic. Nevertheless, two haemolysin (Hly) plasmids of E. coli belonging to the inc groups incFllI,lv (pSU316) and incIz (pHly152) were able to be introduced into these strains by conjugation and stably maintained. A considerable percentage of the Hly+ transconjugants obtained had lost parts of their O-side chains, a result of selection for the better recipient capability of « semi-rough» variants rather than the direct influence of the Hly+ plasmids themselves. In contrast to the incF1lI1V plasmid pSU316, which exhibited higher conjugation rates with rough recipients, the incIz plasmid pHly152 was accepted best by smooth strains. Transformation with cloned E. coli haemolysin (hly) determinant was inefficient ( <10-8) for smooth strains, but 102-103 times higher for rough recipients, and was increased by the use of Salmonella-modified DNA. The transform ants and transconjugants were relatively stable and showed the same haemolytic activity as the E. coli donor strains. The virulence of the Hly+ smooth, semi-rough and rough S. typhimurium strains was tested in two mouse models, and neither the mortality rate nor the ability to multiply within the mouse spleen was influenced by the hly determinants.}, language = {en} } @article{KoenigSchefferBremmetal.1985, author = {K{\"o}nig, W and Scheffer, J. and Bremm, K. D. and Hacker, J{\"o}rg and Goebel, W.}, title = {The role of bacterial adherence and toxin production from E. coli on leukotriene generation from human polymorphonuclear granulocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40295}, year = {1985}, abstract = {No abstract available}, language = {en} } @article{KnappHackerThenetal.1984, author = {Knapp, Stefan and Hacker, J{\"o}rg and Then, Irene and M{\"u}ller, Dorothee and Goebel, Werner}, title = {Multiple copies of hemolysin genes and associated sequences in the chromosome of uropathogenic Escherichia coli strains}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40278}, year = {1984}, abstract = {The 06 serogroup Escherichia coli strain 536 carries two hemolysin (hly) determinants integrated into the chromosome. The two hly determinants are not completely identical, either functionally or structurally, as demonstrated by spontaneous deletion mutants carrying only one of them and by cloning each of the two determinants separately into cosmid vectors. Each hly determinant is independently deleted at a frequency of 10-4 , leading to variants which exhibit similar levels of internal hemolysin but different amounts of secreted hemolysin. The two hly determinants were also identified in the 04 E. coli strain 519. The three E. coli strains 251, 764, and 768, which belong to the serogroup 018, and the 04 strain 367 harbor a single chromosomal hly determinant, as demonstrated by hybridization with hly-gene-specific probes. However, a hybridization probe derived from a sequence adjacent to the hlyC-proximal end of the plasmid pHlyl52-encoded hly determinant hybridizes with several additional chromosomal bands in hemolytic 018 and 06 E. coli strains and even in E. coli K-12. The size ofthe probe causing the multiple hybridization suggests a 1,500- to 1,800-base pair sequence directly flanking hlyC. Spontaneous hemolysin-negative mutants were isolated from strains 764 and 768, which had lost the entire hly determinant but retained all copies of the hlyC-associated sequence. This sequence is not identical to a previously identified (J. Hacker, S. Knapp, and W. Goebel, J. Bacteriol. 154:1145-1154, 1983) somewhat smaller (about 850 base pairs) sequence flanking the other (hlyBb-proximal) end of the plasmid pHlyl52-encoded hly determinant which, as shown here, exists also in multiple copies in these hemolytic E. coli strains and in at least two copies in E. coli K-12. In contrast to the plasmid-encoded hly determinant which is directly flanked at both ends by these two diJJerent sequences, the chromosomal hly determinants are not immediately flanked by such sequences.}, language = {en} } @article{HackerKnappGoebel1983, author = {Hacker, J{\"o}rg and Knapp, S. and Goebel, W.}, title = {Spontaneous deletions and flanking regions of the chromosomal inherited hemolysin determinant of an Escherichia coli 06 strain}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40260}, year = {1983}, abstract = {The hemolytic Escherichia coli strain 536 (06) propagates spontaneous hemolysin- negative mutants at relatively high rates (10-3 to 10-4 ). One type of mutant (type I) lacks both secreted (external) and periplasmic (internal) hemolysin activity (HlYex - IHlYin -) and in addition shows no mannose-resistant hemagglutination (Mrh -), whereas the other type (type II) is HlYex -IHIYin + and Mrh +. The genetic determinants for hemolysin production (hly) and for mannose-resistant hemagglutination (mrh) of this strain are located on the chromosome. Hybridization experiments with DNA probes specific for various parts of the hly determinant reveal that mutants of type I have lost the total hly determinant, whereas those of type 11 lack only part of the hlyB that is essential for transport of hemolysin across the outer membrane. Using a probe that contains the end sequence of the plasmid pHly152-encoded hly determinant (adjacent to hlyB), we determined that a related sequence flanks also the hlyB-distal end of the chromosomal hly determinant of E. coli 536. In addition several other similar or even identical sequences are found in the vicinity of the hlyC- and the hlyB-distal ends of both the chromosomal and the plasmid hly determinants.}, language = {en} } @article{BergerHackerJuarezetal.1982, author = {Berger, Harald and Hacker, J{\"o}rg and Juarez, Antonio and Hughes, Colin and Goebel, Werner}, title = {Cloning of the chromosomal determinants encoding hemolysin production and mannose-resistant hemagglutination in Escherichia coli}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40255}, year = {1982}, abstract = {We have cloned the chromosomal hemolysin determinants from Escherichia coli strains belonging to the four O-serotypes 04, 06, 018, and 075, The hemolysin-producing clones were isolated from gene banks of these strains which were constructed by inserting partial Sau3A fragments of chromosomal DNA into the cosmid pJC74. The hemolytic cosmid clones were relatively stable. The inserts were further sub cloned either as Sail fragments in pACYC184 or as BamHI-SaLI fragments in a recombinant plasmid (pANN202) containing cistron C (hlye) of the plasmid-encoded hemolysin determinant. Detailed restriction maps of each of these determinants were constructed, and it was found that, despite sharing overall homology, the determinants exhibited minor specific differences in their structure, These appeared to be restricted to cistron A (hlyA), which is the structural gene for hemolysin. In the gene banks of two of these hemolytic strains, we could also identify clones which carried the genetic determinants for the mannose-resistant hemagglutination antigens Vb and VIc. Both of these fimbrial antigens were expressed in the E. coli K-12 clones to an extent similar to that observed in the wild-type strains. These recombinant cosmids were rather unstable, and, in the absence of selection, segregated at a high frequency.}, language = {en} } @article{HofEmmerlingHackeretal.1982, author = {Hof, H. and Emmerling, P. and Hacker, J{\"o}rg and Hughes, C.}, title = {The role of macrophages in primary and secondary infection of mice with Salmonella typhimurium}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40248}, year = {1982}, abstract = {Elimination of macrophages with high-molecular dextran sulphate (OS) markedly impairs resistance of mice to primary infection with smooth, virulent strains of Salmonella typhimurium, whereas stimulation of this system by killed Bordetella pertussis organisms increases resistance. In infection with rough, avirulent strains of S. iyphimurium the elimination of macro phages was not followed by an essential loss of resistance, and it appears that other non-specific defence mechanisms, for example the complement system, may have compensated for the lack of macrophages. Macrophages, therefore, play an important role in defence during primary infection with virulent strains. In immunity to challenge infection with S. typhimurium, macrophages play an even more significant role. Treatment with OS completely removes immunity, and both humoral and cell-mediated immune mechanisms seem to require the participation of macrophages.}, language = {en} } @article{StrackDeutsch2004, author = {Strack, Fritz and Deutsch, Roland}, title = {Reflective and Impulsive Determinants of Social Behavior}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40447}, year = {2004}, abstract = {This article describes a 2-systems model that explains social behavior as a joint function of reflective and impulsive processes. In particular, it is assumed that social behavior is controlled by 2 interacting systems that follow different operating principles. The reflective system generates behavioral decisions that are based on knowledge about facts and values, whereas the impulsive system elicits behavior through associative links and motivational orientations. The proposed model describes how the 2 systems interact at various stages of processing, and how their outputs may determine behavior in a synergistic or antagonistic fashion. It extends previous models by integrating motivational components that allow more precise predictions of behavior. The implications of this reflective-impulsive model are applied to various phenomena from social psychology and beyond. Extending previous dual-process accounts, this model is not limited to specific domains of mental functioning and attempts to integrate cognitive, motivational, and behavioral mechanisms.}, subject = {Psychologie}, language = {en} } @article{Scheer1987, author = {Scheer, Ulrich}, title = {Structure of lampbrush chromosome loops during different states of transcriptional activity as visualized in the presence of physiological salt concentrations}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39304}, year = {1987}, abstract = {Lampbrush chromosomes of amphibian oocytes were isolated in the presence of near-physiological salt concentrations, to preserve their native state, and studied by electron microscopy of ultrathin s~dions. The transcriptional state of the lampbrush chromosomes was experimentally modulated by incubating the oocytes for various time periods in medium containing actinomycin D. The observations show that the structure of the lateral loops changes rapidly in response to alterations in transcriptional activity. During decreasing transcriptional activity and reduced packing density of transcripts, the chromatin axis first condensed into nucleosomes and then into an approximately 30 nm thick higher order chromatin fiber. Packaging of the loop axis into supranucleosomal structures may contribute to the foreshortening and retraction of the loops observed during inhibition of transcription and in later stages of meiotic prophase. The increasing packing density of the DNA during the retraction process of the loops could also be visualized by immunofluorescence microscopy using antibodies to DNA. The dependence of the loop chromatin structure on transcriptional activity is discussed in relation to current views of mechanisms involved in gene activation.}, language = {en} } @article{ThiryScheerGoessens1988, author = {Thiry, Marc and Scheer, Ulrich and Goessens, Guy}, title = {Localization of DNA within Ehrlich tumour cells nucleoli by immunoelectron microscopy}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39327}, year = {1988}, abstract = {The distribution of DNA in Ehrlich tumour cell nucleoli was investigated by means of an immunocytochemical approach , involving a monoclonal antibody directed against double- and single-stranded DNA. Immunolabelling was performed . either before or after the embedding process. The postembedding labelling method allows better ultrastructural preservation than the preembedding labelling method. In particular, the various nucleolar components are well preserved and identifiable. In the nucleolus, labelling is particularly concentrated over the perinucleolar chromatin and over its intranucleolar invaginations, which penetrate the nucleolar body and often terminate at the fibrillar centres. In addition, aggregates of gold particles are found in the fibrillar centres, preferentially towards the peripheral regions. By contrast, the dense fibrillar component is completely devoid of labelling. The results seem to indicate that DNA containing the rDNA genes is located in the fibrillar centres, with a preference for the peripheral regions. This finding suggests that transcription of the rDNA genes should occur within the confines of the fibrillar centre, probably close to the boundary region of the surrounding dense fibrillar component. The results are discussed in the light of present knowledge of the functional organization of the nucleolus.}, language = {en} } @article{BenaventeSchmidtZachmannHuegleDoerretal.1988, author = {Benavente, Ricardo and Schmidt-Zachmann, Marion S. and H{\"u}gle-D{\"o}rr, B. and Reimer, G. and Rose, K. M. and Scheer, Ulrich}, title = {Identification and definition of nucleolus-related fibrillar bodies in micronucleated cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39423}, year = {1988}, abstract = {Small nucleolus-related bodies which occur in the nUcleoplasm of " micronuclei" lacking nucleolar organizers have been studied by immunofluorescence microscopy. These bodies stained specifically with three different antibodies directed against proteins that are normally associated with the dense fibrillar component of functional nucleoli, but not with antibodies specific for certain proteins of the granular component or the fibrillar centers. Our data show that, in the absence of rRNA genes, the various constituent proteins characteristic of the dense fibrillar component spontaneously assemble into spherical entities but that the subsequent fusion of these bodies into larger structures is prevented in these micronuclei. The similarity between these nucleolus-related bodies of micronuclei and the prenucleolar bodies characteristic of early stages of nucleologenesis during mitotic telophase is discussed.}, language = {en} } @article{FrankeScheerHerth1974, author = {Franke, Werner W. and Scheer, Ulrich and Herth, Werner}, title = {Cytology, general and molecular cytology}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39499}, year = {1974}, abstract = {The present article had originally been conceived as a review on endomembranes, the plasma membrane, and the major product of membrane-bound activities, the cell wall material. However, limitations of space and the cascading number of pertinent literature articles made it necessary to confine this to one group of membranes and one type of cell wall components. Therefore, we shall begin our survey on the biochemical and cytological aspects of membranes by a review of the class of the pore complex bearing endomembranes, i.e. the nuclear envelope and the annulate lamellae (AL). Next year the membranes of the endoplasmic reticulum and the dictyosomes will be dealt with in conjunction with a discussion of the various intracellular vesicles, the tonoplast and the plasmalemma.}, subject = {Botanik}, language = {en} } @article{Scheer1972, author = {Scheer, Ulrich}, title = {The ultrastructure of the nuclear envelope of amphibian ooctyes: IV. On the chemical nature of the nuclear pore complex material}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39500}, year = {1972}, abstract = {In order to investigate the chemical composition of the nuclear pore complexes isolated nuclei from mature Xenopus laevis oocytes were manually fractioned into nucleo· plasmic aggregates and the nuclear envelopes. The whole isolation procedure takes no more than 60- 90 sec, and the pore complexes of the isolated envelopes are well preserved as demonstrated by electron microscopy. Minor nucleoplasmic and cytoplasmic contaminations associated with the isolated nuclear envelopes were determined with electron microscopic morphometry and were found to be quantitatively negligible as far as their mass and nucleic acid content is concerned. The RNA content of the fractions was determined by direct phosphorus analysis after differential alkaline hydrolysis. Approximately 9\% of the total nuclear RNA of the mature Xenopus egg was found to be attached to the nuclear envelope. The nonmembranous elements of one pore complex contain 0.41 X 10- 16 g RNA. This value agrees well with the content estimated from morphometric data. The RNA package density in the pore complexes (270 X 10- 15 g/fJ-3) is compared with the nucleolar, nucleoplasmic and cytoplasmic RNA concentration and is discussed in context with the importance of the pore complexes for the nucleo-cytoplasmic transport of RNA-containing macromolecules. Additionally, the results of the chemical analyses as well as of the 3H-actinomycin D autoradiography and of the nucleoprotein staining method of Bernhard (1969) speak against the occurence of considerable amounts of DNA in the nuclear pore complex structures.}, language = {en} } @article{FrankeKartenbeckKrienetal.1972, author = {Franke, Werner W. and Kartenbeck, J{\"u}rgen and Krien, S. and VanderWoude, W. J. and Scheer, Ulrich and Morr{\´e}, D. J.}, title = {Inter- and intracisternal elements of the Golgi apparatus: A system of membrane-to-membrane cross-links}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39514}, year = {1972}, abstract = {Electron opaque cross-bridge structures span the inter- and intracisternal spaces and provide membrane-to-membrane connections between adjacent cisternae of dictyosomes of pollen tubes of Clivia and Lilium. Additionally, the classic intercisternal rods, characteristic of intercisternal regions near the maturing face of dictyosomes, are connected with the adjacent membranes through similar cross-bridge elements. We suggest that these structural links are responsible for maintaining the flattened appearance of the central parts of Golgi apparatus cisternae as well as for the coherence of cisternae within the stack. Observations on other plant (e.g. microsporocytes of Canna) and animal cells (e.g. rodent liver and hepatoma cells, newt spermatocytes) show that such an array of membrane cross-links is a universal feature of Golgi apparatus architecture. The cross-bridges appear as part of the complex "zone of exclusion" which surrounds dictyosomes, entire Golgi apparatus and Golgi apparatus equivalents in a variety of cell types.}, language = {en} } @article{ScheerKartenbeckTrendelenburgetal.1976, author = {Scheer, Ulrich and Kartenbeck, J{\"u}rgen and Trendelenburg, Michael F. and Stadler, Joachim and Franke, Werner W.}, title = {Experimental disintegration of the nuclear envelope: evidence for pore-connecting fibrils}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39735}, year = {1976}, abstract = {The disintegration of the nuclear envelope has been examined in nuclei and nuclear envelopes isolated from amphibian oocytes and rat liver tissue, using different electron microscope techniques (ultrathin sections and negatively or positively stained spread preparations). Various treatments were studied, including disruption by surface tension forces, very low salt concentrations, and non ionic detergents such as Triton X-lOO and Nonidet P-40. The high local stability of the cylinders of nonmembranous pore complex material is emphasized. As progressive disintegration occurred in the membrane regions, a network of fibrils became apparent which interconnects the pore complexes and is distinguished from the pore complexassociated intranuclear fibrils. This network might correspond to an indistinct lamella, about 15 - 20 nm thick, located at the level of the inner nuclear membrane, which is recognized in thin sections to bridge the interpore distances. With all disintegration treatments a somewhat higher susceptibility of the outer nuclear membrane is notable, but a selective removal does not take place. Final stages of disintegration are generally characterized by the absence of identifiable, membrane- like structures. Analysis of detergent-treated nuclei and nuclear membrane fractions shows almost complete absence of lipid components but retention of significant amount of glycoproteins with a typical endomembrane-type carbohydrate pattern. Various alternative interpretations of these observations are discussed. From the present observations and those of Aaronson and Blobel (1,2), we favor the notion that threadlike intrinsic membrane components are stabilized by their attachment to the pore complexes, and perhaps also to peripheral nuclear structures, and constitute a detergent-resistant, interpore skeleton meshwork.}, language = {en} } @article{TrendelenburgScheerFranke1973, author = {Trendelenburg, Michael F. and Scheer, Ulrich and Franke, W. W.}, title = {Structural organization of the transcription of ribosomal DNA in oocytes of the house cricket}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33113}, year = {1973}, abstract = {No abstract available}, language = {en} } @article{ScheerRaska1987, author = {Scheer, Ulrich and Raska, I.}, title = {Immunocytochemical localization of RNA polymerase I in the fibrillar centers of nucleoli}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39618}, year = {1987}, abstract = {No abstract available}, language = {en} } @article{FischerHockScheer1993, author = {Fischer, Dagmar and Hock, Robert and Scheer, Ulrich}, title = {DNA Topoisomerase II is not detectable on lampbrush chromosomes but enriched in the amplified nucleoli of xenopus oocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32654}, year = {1993}, abstract = {In somatic cells DNA topoisomerase II (topo II) is thought to be involved in the domain Organization of the genome by anchoring the basis of chromatin loops to a chromosomal scafFold. Lampbrush chromosomes of am-phibian oocytes directly display this radial loop Organization in cytological preparations. In order to find out whether topo II may play a role in the Organization of these meiotic chromosomes, we performed immunofluorescence studies using antibodies against Xenopus topo II. Our results indicate that topo II is apparently absent from lampbrush chromosomes and is hence unlikely to act as a "fastener" of the numerous lateral chromosomal loops. Topo II was, however, enriched in the amplified nucleoli of Xenopus oocytes.}, language = {en} } @article{FrankeScheer1972, author = {Franke, Werner W. and Scheer, Ulrich}, title = {Structural details of dictyosomal pores}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32155}, year = {1972}, abstract = {Structural details of the dictyosomal pores in several plant cell types are described from tangential and cross sections of Golgi cisternae. Frequency distributions of the sizes of such Golgi pores are given and compared with the corresponding values of nuclear pores in the same cells. Golgi pore inner diameters are less homogeneously distributed and can be as small as 100 A or less. They are not simply cisterna I holes, but are often associated with centrally located electron dense granules or rods and with inner pore filaments. This organization, which is very common in dictyosomal pores in plant and animal cells, has some similarities with the structural architecture of nuclear envelope and annulate lamellar pore complexes. The particulate material associated with the dictyosomal pores shows spatial and structural relationship to cytoplasmic ribosomes. Possible modes of Golgi pore formation and some consequences of these observations for interpretation of nuclear pore structures are discussed.}, language = {en} } @article{FrankeScheerSpringetal.1976, author = {Franke, Werner W. and Scheer, Ulrich and Spring, Herbert and Trendelenburg, Michael F. and Krohne, G.}, title = {Morphology of transcriptional units of rDNA: evidence for transcription in apparent spacer intercepts and cleavages in the elongating nascent RNA}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39681}, year = {1976}, abstract = {Several types of "irregular" structures in the arrangement of lateral fibrils were noted in electron microscopic preparations of transcriptionally active nucleolar chromatin from various plant and animal cells. Such forms include: I. Disproportionately long lateral fibrils which occur either as individual fibrils or in groups; 2. "Prelude complexes" and other arrangements of lateral fibrils in apparent spacer intercepts; 3. Thickening of the rDNA chromatin axis at the starting end of pre-rRNA matrix units; 4. Extremely long matrix units , the length of which exceeds that of the rDNA (double-strand) sequence complementary to the specific pre-rRN A (for abbreviations see text). In addition, the stability of high molecular weight RNAs contained in the nucleolar ribonucleoproteins during the preparation for electron microscopy was demonstrated by gel electrophoresis. The observations indicate that the morphological starting point of a pre-rRNA matrix unit is not necessarily identical with the initiation site for synthesis of pre-rRNA, but they rather suggest that the start of the transcriptional unit is located at least O.2-D.8 JLm before the matrix unit and that parts of the "apparent spacer" are transcribed. It is proposed that the pre-rRN A molecules do not represent the primary product of rDNA transcription but rather relatively stable intermediate products that have already been processed during transcription.}, language = {en} } @article{BringmannZagstSchoeneretal.1991, author = {Bringmann, Gerhard and Zagst, Rainer and Sch{\"o}ner, Bernd and Busse, Holger and Hemmerling, Martin and Burschka, Christian}, title = {Acetogenic Isoquinoline Alkaloids. XXIII. Structure of the Naphthyl Isoquinoline Alkaloid Dioncophylline A}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31331}, year = {1991}, abstract = {No abstract available}, subject = {Chemie}, language = {en} } @article{ScheerSommervilleMueller1980, author = {Scheer, Ulrich and Sommerville, John and M{\"u}ller, Ulrike}, title = {DNA is assembled into globular supranucleosomal chromatin structures by nuclear contents of amphibian oocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39671}, year = {1980}, abstract = {The assembly of DNA into nucleosomal and supranucleosomal chromatin structures has been studied (i) by injection of circular DNA molecules (plasmids) into nuclei of Pleurodeles waltlii oocytes; and (ii) by in vitro incubation of plasmid molecules with the supernatant fraction from oocyte nuclei of Pleurodeles and Xenopus laevis, followed by purification of nucleoprotein structures formed with sucrose gradient centrifugation. [n both types of experiments , spread preparations of the newly assembled and transcriptionally inactive chromatin , examined by electron microscopy , show dense globular higher order (supranucleosomal) packing forms. Under partially relaxing (low salt) preparation conditions granular chromatin subunits of about 30 nm diameter can be seen either as widely spaced particles or in closely packed aggregates. The transcriptionally inactive endogenous chromatin of chromomeres of lampbrush chromosomes is arranged in similar higher order chromatin units. A correlation is found between the sizes of the DN A molecule probes used and the numbers of nucleosomes and higher order globules in the assembled chromatin structures. After prolonged dispersion in low salt buffers , these globular chromatin units unfold into chains of7-12 nucleosomes. The results support the concept that chromatin is arranged , under physiological ion concentrations as they are present in the nucleus , in supranucleosomal units of globular morphology.}, language = {en} } @article{HuegleHazanScheeretal.1985, author = {H{\"u}gle, Barbara and Hazan, Rachel and Scheer, Ulrich and Franke, Werner W.}, title = {Localization of ribosomal protein S1 in the granular component of the interphase nucleolus and its distribution during mitosis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39695}, year = {1985}, abstract = {Using antibodies to various nucleolar and ribosomal proteins, we define, by immunolocalization in situ, the distribution of nucleolar proteins in the different morphological nucleolar subcompartments. In the present study we describe the nucleolar localization of a specific ribosomal protein (51) by immunofluorescence and immunoelectron microscopy using a monoclonal antibody (R5 1-105). In immunoblotting experiments, this antibody reacts specifically with the largest and most acidic protein of the small ribosomal subunit (51) and shows wide interspecies cross-reactivity from amphibia to man. Beside its localization in cytoplasmic ribosomes, this protein is found to be specifically localized in the granular component of the nucleolus and in distinct granular aggregates scattered over the nucleoplasm. This indicates that ribosomal protein 51, in contrast to reports on other ribosomal proteins, is not bound to nascent pre-rRNA transcripts but attaches to preribosomes at later stages of rRNA processing and maturation. This protein is not detected in the residual nucleolar structures of cells inactive in rRNA synthesis such as amphibian and avian erythrocytes. During mitosis, the nucleolar material containing ribosomal protein 51 undergoes a remarkable transition and shows a distribution distinct from that of several other nucleolar proteins. In prophase, the nucleolus disintegrates and protein 51 appears in numerous small granules scattered throughout the prophase nucleus. During metaphase and anaphase, a considerable amount of this protein is found in association with the surfaces of all chromosomes and finely dispersed in the cell plasm. In telophase, protein 51-containing material reaccumulates in granular particles in the nucleoplasm of the newly formed nuclei and, finally, in the re-forming nucleoli. These observations indicate that the nucleolus-derived particles containing ribosomal protein 51 are different from cytoplasmic ribosomes and, in the living cell, are selectively recollected after mitosis into the newly formed nuclei and translocated into a specific nucleolar subcompartment, i.e ., the granular component. The nucleolar location of ribosomal protein 51 and its rearrangement du'ring mitosis is discussed in relation to the distribution of other nucleolar proteins.}, subject = {Cytologie}, language = {en} } @article{ScheerHinssenFrankeetal.1984, author = {Scheer, Ulrich and Hinssen, Horst and Franke, Werner W. and Jockusch, Brigitte M.}, title = {Microinjection of actin-binding proteins and actin antibodies demonstrates involvement of nuclear actin in transcription of lampbrush chromosomes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39706}, year = {1984}, abstract = {Nuclei of amphibian oocytes contain large amounts of actin, mostly in unpolymerized or short-polymer form. When antibodies to actin or actin-binding proteins (fragmin and the actin modulator from mammalian smooth muscle) are injected into nuclei of living oocytes of Pleurodeles waltlii, transcription of the lampbrush chromosomes, but not of the rRNA genes, is inhibited. When transcription is repressed by drugs or RNA is digested by microinjection of RNAase into oocyte nuclei, an extensive meshwork of actin filament bundles is seen in association with the isolated lampbrush chromosomes. These observations indicate a close relationship between the state of nuclear actin and transcriptional activity and suggest that nuclear actin may be involved in transcriptional events concerning protein-coding genes.}, language = {en} } @article{ScheerHansmannFalketal.1986, author = {Scheer, Ulrich and Hansmann, Paul and Falk, Heinz and Sitte, Peter}, title = {Ultrastructural localization of DNA in two Cryptomonas species by use of a monoclonal DNA-antibody}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39746}, year = {1986}, abstract = {Immunogold cytochemistry - DNA localization - Cryptomonas nucleomorph The distribution and subcellular localization of DNA in the unicellular alga Cryptomonas has been investigated electron-microscopically by indirect immunocytochemistry, using a monoclonal DNA antibody and a gold-Iabeled secondary antibody. This technique proved to be very sensitive and entirely specific. DNA could be demonstrated in four different compartments (nucleus, nucleomorph, plastid, and mitochondrion). Within the plastid, DNA is concentrated in stroma regions that are localized preferentially around the center of the organelle. The mitochondrion contains several isolated DNA-containing regions (nucleoids). Within the nucleus, most of the DNA is localized in the 'condensed' chromatin. DNA was also detectable in small areas of the nucleolus, whereas the interchromatin space of the nucleus appeared almost devoid of DNA. Within the nucleomorph, DNA is distributed inhomogeneously in the matrix. DNA could furthermore be detected in restricted areas of the 'fibrillogranular body' of the nucleomorph, resembling the situation encountered in the nucleol us. The presence of DNA and its characteristic distribution in the nucleomorph provide additional, strong evidence in favour of the interpretation of that organelle as the residual nucleus of a eukaryotic endosymbiont in Cryptomonas.}, subject = {Cytologie}, language = {en} } @article{Scheer1978, author = {Scheer, Ulrich}, title = {Changes of nucleosome frequency in nucleolar and non-nucleolar chromatin as a function of transcription: an electron microscopic study}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39750}, year = {1978}, abstract = {The morphology of nucleolar and non-nucleolar (Iampbrush chromosome loops) chromatin was studied in the electron microscope during states of reduced transcriptional activity in amphibian oocytes (Xenopus laevis, Triturus alpestris, T. cristatus). Reduced transcriptional activity was observed in maturing stages of oocyte development and after treatment with an inhibitor, actinomycin D. Strands of nucleolar chromatin appear smooth and thin, and contain only few, if any, nucleosomal particles in the transcribed units. This is true whether they are densely or only sparsely covered with lateral ribonucleoprotein fibrils. This smooth and non-nucleosomal character is also predominant in the interspersed, apparently nontranscribed rDNA spacer regions. During inactivation, however, nucleolar chromatin frequently and progressively assumes a beaded appearance in extended fibril-free-that is, apparently nontranscribed - regions. I n either fUll-grown 00- cytes or late after drug treatment, most of the nucleolar chromatin is no longer smooth and thin, but rather shows a beaded configuration indistinguishable from inactive non - nucleolar chromatin. In many chromatin strands, transitions of fibril-associated regions of smooth character into beaded regions wihout lateral fibrils are seen. Similarly, in the non-nucleolar chromatin of the retracting lampbrush chromosome loops, reduced transcriptional activity is correlated with a change from smooth to beaded morphology. Here, however, beaded regions are also commonly found interspersed between the more or less distant bases of the lateral fibrils, the putative transcriptional complexes. I n both sorts of chromatin, detergents (in particular Sarkosyl) that remove most of the chromatin proteins including histones from the DNA axis but leave the RNA polymerases of the transcriptional complexes attached were used to discriminate between polymerases and nucleosomal particles. The results suggest that nucleosomes are absent in heavily transcribed chromatin regions but are reformed after inactivation. In contrast to the findings with inactivated nucleolar genes, in lampbrush chromosome loops the beaded nucleosomal configuration appears to be assumed also in regions within transcriptional units that, perhaps temporarily, are not involved in transcription.}, language = {en} } @article{ScheerSommerville1981, author = {Scheer, Ulrich and Sommerville, J.}, title = {Structural organization of nascent transcripts and hnRNA molecules in amphibian oocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39765}, year = {1981}, abstract = {Comparisons ofrelative lengths oflampbrush loops, nascent RNP transcripts and hnRNA molecules from oocytes of amphibia with different C-values show that there is an increasing trend in loop, and transcriptional unit, length with increase in genome size but no increasing trend with respect to RN A contour length.The formation of duplex regions and circles in RNP fibrils indicates that RNA processing may occur within the nascent fibrils. The hnRNA molecules from oocytes of the various amphibia readily form intermolecular duplex structures. These complementary sequences have a low kinetic complexity and are transcribed from highly repetitive sequences distributed throughout the genome. Their possible function is considered.}, language = {en} } @article{ScheerSchmidtZachmannHuegleetal.1984, author = {Scheer, Ulrich and Schmidt-Zachmann, Marion S. and H{\"u}gle, Barbara and Franke, Werner W.}, title = {Identification and localization of a novel nucleolar protein of a high molecular weight by a monoclonal antibody}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39786}, year = {1984}, abstract = {A monoclonal murine antibody (No-I 14) is described which reacts specifically with a polypeptide of molecular weight (M,) 180000 present in low-speed nuclear pellets from oocytes and somatic cells of Xenopus laevis and X. borealis and in isolated amplified nucleoli. Two-dimensional gel electrophoresis has revealed the acidic nature of this polypeptide (isoelectric at pH of ca 4.2 in the presence of 9.5 M urea). A relatively large proportion of the protein is extracted at elevated ionic strength( i.e., at 0.4-0.5 M alkali salt) in a form sedimenting at approx. 7-8S , compatible with a monomeric state. It is also extracted by digestion with RNase but not with DNase. In immunofluorescence microscopy, antibody No-114 stains intensely nucleoli of oocytes and all somatic cells examined , including the residual nucleolar structure of Xenopus erythrocytes which are transcriptionally inactive. During mitosis the antigen does not remain associated with the nucleolar organizer regions (NOR) of chromosomes but is released and dispersed over the cytoplasm until telophase when it re-associates with the reforming interphase nucleoli. At higher resolution the immunofluorescent region is often resolved into a number of distinct subnucleolar components of varied size and shape. Immunoelectron microscopy using colloidal gold-coupled secondary antibodies reveals that the M, 180000 protein is confined to the dense fibrillar component of the nucleolus. This conclusion is also supported by its localization in the fibrillar part of segregated nucleoli of cells treated with actinomycin D. We conclude that nucleoli contain a prominent protein of M, 180000 which contributes to the general structure of the dense fibrillar component of the interphase nucleolus , independent of its specific transcriptional activity.}, language = {en} } @article{ThiryScheerGoessens1991, author = {Thiry, Marc and Scheer, Ulrich and Goessens, Guy}, title = {Localization of nucleolar chromatin by immunocytochemistry and in situ hybridization at the electron microscopic level}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-39289}, year = {1991}, abstract = {Nucleoli are the morphological expression of the activity of a defined set of chromosomal segments bearing rRNA genes. The topological distribution and composition of the intranucleolar chromatin as well as the definition of nucleolar structures in which enzymes of the rDNA transcription machinery reside have been investigated in mammalian cells by various immunogold labelling approaches at the ultrastructural level. The precise intranucleolar location of rRNA genes has been further specified by electron microscopic in situ hybridization with a non-autoradiographic procedure. Our results indicate that the fibrillar centers are the sole nucleolar structures where rDNA, core histones, RNA polymerase I and DNA to po isomerase I are located together. Taking into account the potential value and limitations of immunoelectron microscopic techniques, we propose that transcription of the rRNA genes takes place within the confines of the fibrillar centers, probably close to the boundary regions to the surrounding dense fibrillar component.}, language = {en} } @article{GesslerKoenigArdenetal.1994, author = {Gessler, Manfred and K{\"o}nig, A. and Arden, K. and Grundy, P. and Orkin, S. H. and Sallan, S. and Peters, C. and Ruyle, S. and Mandell, J. and Li, F. and Cavenee, W. and Bruns, G. A.}, title = {Infrequent mutation of the WT1 gene in 77 Wilms' Tumors}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34308}, year = {1994}, abstract = {Homozygous deletions in Wilms' tumor DNA have been a key step in the identification and isolation of the WTI gene. Several additional loci are also postulated to contribute to Wilms' tumor formation. To assess the frequency of WTI alterations we have analyzed the WTI locus in a panel of 77 Wilms' tumors. Eight tumors showed evidence for large deletions of several hundred or thousand kilobasepairs of DNA, some of which were also cytogenetically detected. Additional intragenic mutations were detected using more sensitive SSCP analyses to scan all 10 WTI exons. Most of these result in premature stop codons or missense mutations that inactivate the remaining WTI allele. The overall frequency of WTI alterations detected with these methods is less than 15\%. While some mutations may not be detectable with the methods employed, our results suggest that direct alterations of the WTI gene are present in only a small fraction of Wilms' tumors. Thus, mutations at other Wilms' tumor loci or disturbance of interactions between these genes likely play an important role in Wilms' tumor development.}, language = {en} } @article{FrankeScheer1970, author = {Franke, Werner W. and Scheer, Ulrich}, title = {The ultrastructure of the nuclear envelope of amphibian oocytes: a reinvestigation. I. The mature oocyte}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32098}, year = {1970}, abstract = {1 n order to review the contradictory statements on the ultrast ructure of the nuclear envelope, a study was undertaken combining section and negat ive stai ning electron microscopy on manually isolated oocyte nuclei and nuclear envelopes from six amphibian species including Anura as well as Urodela. The a ppeara nce of the negatively stained iso lated nuclear envelopes is described in deta il and the dependence on the preparation co nditions used is emphas ized . Pore complex structures such as pore perimeter, central granule, an nul ar components, interna l fibrils, and annu lus-attached fibrils could be identified by both techniques, negat ive staining and sect ions. Comparative studies show that no marked diffe rences ex ist in the structural data of the nuclear envelope among the investigated amphibians and the significance of the structural components is discussed. A model of the nuclea r pore complex based on the findings of the present investigation is prese nted.}, language = {en} } @article{QuastSchmittSchaeferetal.1994, author = {Quast, Helmut and Schmitt, Edeltraud and Sch{\"a}fer, Peter and Heller, Eberhard and Aldenkortt, Sven}, title = {Synthesis and Thermolysis of a Chiral, Non-Racemic Iminoaziridine}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-38298}, year = {1994}, abstract = {The 2-halo imidoyl chlorides 7 are obtained from the amide 5 and the 2-halo amides 6 by the action of phosphorus pentachloride and thionyl chloride, respectively. Non-racemic (S)-6a is converted into 7a which is racemic, however. The reaction of Lawesson's reagent with 6a furnishes the diastereomeric 1,3.2-thiazaphospholidine derivatives 15. Treatment of (S)-6a (98\% eel with methyl triflate affords 2-chloro imidate 8 (95\% eel which reacts with methanamine in the presence of methanammonium chloride to yield the 2-chloro amidine (S)-9a (90\% eel. The 2-halo imidoyl halides 7a and b react with methanamine to produce the 2-halo amidines 9a and b. - Strong bases, e.g. potassium tert-butoxide or sodium hydride in the presence of catalytic amounts of tertbutyl alcohol, eliminate hydrogen chloride or bromide from the 2-halo amidines 9a and band (S)-9a to yield mixtures of Recently, we demonstrated that the formation of the chiral non-racemic aziridinone (R)-2 from the a-chloro amide (5)-1 by base-promoted dehydrochlorination[2) as well as the nucleophilic cleavage of the N-C(3) bond of (R)_2[3,4) occur with inversion of configuration, thus excluding the intervention of achiral (acyclic) intermediates. In the temperature range of lOO-170°C, however, slow racemization accompanies the thermolysis of (R)-2 and indicates the existence of an achiral or a racemic transient, e. g. (M)-3 + (P)-3. Indeed, high-level quantum-chemical calculations reveal that an activation energy of (170 ± 25) kJmol- 1 is required for the unimolecular ring opening of the parent aziridinone which affords a species of high diradical character[41. Subsequently, the unstable N-phenylaziridinone invoked in the decomposition of the (5)-2-bromopropananilide anion was shown to react with tert-butylamine or dimethylformamide with inversion of configuration at C(3)[51. Thus, the stereochemical evidence in the series of 3-alkylaziridinones excludes achiral (acyclic) aziridinone isomers as intermediates at low tempera tures [6J. Similar stereochemical studies are still missing in the related series of iminoaziridines. Therefore, we report on the synthesis and thermolysis of the diastereomeric chiral racemic (E)- and (Z)-(4)[71 and non-racemic iminoaziridines (E,R)- and (Z,R)-4. Racemic Iminoaziridines (E)- and (Z)-4 Though a photochemical route to the iminoaziridines (E)- and (Z)-4 has been devised more recently, i. e. the phothe 2-iminoaziridines (E)- and (Z)-4, and (E,R)- and (Z.R)-4 (83\% eel, respectively. The 1.3-elimination of hydrogen bromide from 9b is diastereoselective at -30 to -40°C [(E)-4:(Z)-4 = <10:>90). The diastereomers equilibrate at 36°C with (kEZ + k ZE) = (5.92 ± 0.08) . 10-5 S-I (K = kEZlkzE = 0.428 ± 0.013). - The thermolysis of (E)- and (Z)-4 in [D61benzene solution yields the imine 16 and methyl isocyanide (17). The decomposition follows the first-order rate law. The following Arrhenius and Eyring parameters are calculated from five rate constants obtained in the temperature range of 70-110°C: Ea = (115.2 ± 0.4) kJmol-t, IgA = (12.06 ± 0.28), AH* = (112.1 ± 0.4) kJmol- l , AS'" = (-23.9 ± 0.7) JK-I mol-I, AGj73K = 121 kJmol-1 . The enantiomeric excess of the surviving fraction of (E,R)- and (Z.R)-4 is unchanged after two half-lives at 80°C.}, language = {en} } @article{HellerHerdeis1993, author = {Heller, Eberhard and Herdeis, Claus}, title = {Synthesis of 2S,5S- and 2R,5S-5-Hydroxypipecolic Acid via Amide-Methylenation of S-5-Hydroxy-2-piperidone with Dimethyltitanocene}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-38288}, year = {1993}, abstract = {A route to 2S,5S-and 2R,5S-hydroxypipecolic acid is presented, starting with the enantiopure 5S-5-hydroxy-piperidone 7. The key step of this reaction sequence is the chemoselsctive methylenation of the amide carbonyl group of 8 with dimethyltitanocene 9 to 10. The transformation of the exocyclic enecarbamate double bond to the carboxylic acid group is best accomplished via hydroboration/oxidation to the alcohol 11a,b. Separation and oxidation of the dlastereomers 11a,b, to 148. and 14b, and hydrolysis furnishes the diastereomeric pipecolic acids 15a and 15b in enantiopure form.}, subject = {Chemie}, language = {en} } @article{ReimerRaskaScheeretal.1988, author = {Reimer, Georg and Raska, Ivan and Scheer, Ulrich and Tan, Eng M.}, title = {Immunolocalization of 7-2-ribonucleoprotein in the granular component of the nucleolus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33890}, year = {1988}, abstract = {Certain autoimmune sera contain antibodies against a nucleolar ribonucleoprotein particle associated with 7-2-RNA (R. Reddy et al. (1983) J. Bioi. Chem . 258, 1383; C. Hashimoto and J. A. Steitz (1983) J. Bioi. Chem. 258, 1379). In this study, we showed by immunofluorescence microscopy that antibodies reactive with 7-2-ribonucleoprotein immunolocalized in the granular regions of actinomycin D and 5,6-dichloro-I-j3-D-ribofuranosylbenzimidazole (DRB)-segregated nucleoli from Vero cells. By electron microscopic immunocytochemistry, antigen-antibody complexes were located in the granular component of transcriptionally active nucleoli from rat liver hepatocytes and HeLa cells. Anti-7- 2-RNP antibodies from two autoimmune sera immunoprecipitated a major protein of Mr 40,000 from e5S] methionine-Iabeled HeLa cell extract. The immunolocalization data suggest that 7-2-ribonucleoprotein may be involved in stages of ribosome biogenesis which take place in the granular component of the nucleolus, i.e., assembly, maturation, and/or transport of preribosomes}, language = {en} } @article{SommervilleScheer1982, author = {Sommerville, John and Scheer, Ulrich}, title = {Transcription of complementary repeat sequences in amphibian oocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33915}, year = {1982}, abstract = {Repeat sequences are transcribed in the germinal vesicles of amphibian oocytes. In the hnRNA population both complements of the repeats are found and can be readily detected because they form intermolecular duplex structures. The structure and formation of duplex regions have been studied in the hnRNA of Xenopus laevis, Triturus cristatus, Amphiuma means and Necturus maculosus, a series of amphibians of increasing genome size (C-value). In T. cristatus, the duplex structures are mostly 600- 1200 bp in length, whereas in X. laevis they are shorter and in N. maculosus they tend to be longer. Although the proportion of RNA sequence capable of rapidly forming duplex structures is different in different organisms, this property bears no relationship to C-value. However the sequence complexity of complementary repeats, as estimated from the rate of duplex formation, does show an increasing trend with C-value. The complementary repeats found in oocyte hnRNA are transcribed from families of DNA sequence that are each represented in the genome by thousands of copies. The extent of cross-species hybridization is low, indicating that the repeat sequences transcribed in different amphibian genera are not the same. In situ hybridization experiments indicate that the repeat sequences are spread throughout the genome. The evolution and possible function of complementary repeats are considered.}, language = {en} } @article{BenaventeRoseReimeretal.1987, author = {Benavente, Ricardo and Rose, Kathleen M. and Reimer, Georg and H{\"u}gle-D{\"o}rr, Barbara and Scheer, Ulrich}, title = {Inhibition of nucleolar reformation after microinjection of antibodies to RNA polymerase I into mitotic cells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33247}, year = {1987}, abstract = {The formation of daughter nuclei and the reformation of nucleolar structures was studied after microinjection of antibodies to RNA polymerase I into dividing cultured cells (PtK2). The fate of several nucleolar proteins representing the three main structural subcomponents of the nucleolus was examined by immunofluorescence and electron microscopy. The results show that the RNA polymerase I antibodies do not interfere with normal mitotic progression or the early steps of nucleologenesis, i.e. , the aggregation of nucleolar material into prenucleolar bodies. However,they inhibit the telophasic coalescence of the prenucleolar bodies into the chromosomal nucleolar organizer regions, thus preventing the formation of new nucleoli. These prenucleolar bodies show a fibrillar organization that also compositionally resembles the dense fibrillar component of interphase nucleoli . We conclude that during normal nucleologenesis the dense fibrillar component forms from preformed entities around nucleolar organizer regions, and that this association seems to be dependent on the presence of an active form of RNA polymerase I.}, language = {en} } @article{FrankeBergerFalketal.1974, author = {Franke, Werner W. and Berger, S. and Falk, Heinz and Spring, H. and Scheer, Ulrich and Trendelenburg, Michael F. and Schweiger, H. G. and Herth, W.}, title = {Morphology of the nucleo-cytoplasmic interactions during the development of Acetabularia cells. I. The vegetative phase}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32363}, year = {1974}, abstract = {The ultrastructure of th e growin g and ma turing primary nucleus of Acetabularia medite rranea and Acetabularia major has been studied with the use of various fi xation procedures. Particular interest has been focused on the deta ils of the nuclear periphery and the perinuclear region. It is demonstrated that early in nuclear grow th a characteristic perinucl ear structura l complex is formed which is, among the eukaryotic cells, unique to Acetabularia and re lated genera. This perinuclear system consists essentially of a) the nuclear envelope with a very hi gh pore frequency and various pore complex assoc iat ion s w ith granular and/or threadlike structures some of which are continuous with the nucleolus; b) an approx imate ly 100 nm thick intermediate zone densely filled with a filam entOus material and occasional sma ll membraneous structures from which the typical cytOplasmic and nuclear organe lles and particles are excl ud ed ; c) an adjacent Iacunar labyrinthum which is interrupted by many plasmatic junction channels between the intermed iate zone and the free cytOplasm; d) numerous dense perinuclear bodies in the juxtanuclear cytOplasm which a re especia lly frequent at the junction channels and reveal a composition of aggregated fibrillar and granul ar structures; e) very dense exclusively fibrill ar agg regates which occur either in assoc iation with t he perinuclear region of the lacunar labyrinthum or, somewhat further out, in the cytOplasmic strands between the bra nches of the lacun ar labyrinthum in the form of slender, characteristic rods or "sausages".}, language = {en} } @article{Scheer1973, author = {Scheer, Ulrich}, title = {Nuclear pore flow rate of ribosomal RNA and chain growth rate of its precursor during oogenesis of Xenopus laevis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32178}, year = {1973}, abstract = {The number of ribosomal RNA molecules which are transferred through an average nuclear pore complex per minute into the cytoplasm (nuclear pore flow rate, NPFR) during oocyte growth of Xenopus laevis is estimated. The NPFR calculations are based on determinations of the increase of cytoplasmic rRNA content during defined time intervals and of the total number of pore complexes in the respective oogenesis stages. In the mid-la mpbrush stage (500:"700 I'm oocyte diameter) the NPFR is maximal with 2.62 rRNA molecules/ pore/ minute. Then it decreases to zero at the end of oogenesis. The nucleocytoplasmic RNA f10w rates determined are compared with corresponding values of other cell types. The molecular weight of the rRNA precursor transcribed in the extrachromosomal nucleoli of Xenopus lampbrush stage oocytes is determined by acrylamide gel electrophoresis to be 2.5 x 10· daltons. From the temporal increase of cytoplasmic rRNA (3.8 I'g per oocyte in 38 days) and the known number of simultaneously growing precursor molecules in the nucleus the chain growth rate of the 40 S precursor RNA is estimated to be 34 nucleotides per second.}, language = {en} } @article{ScheerFranke1969, author = {Scheer, Ulrich and Franke, Werner W.}, title = {Negative staining and adenosine triphosphatase activity of annulate lamellae of newt oocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32087}, year = {1969}, abstract = {Semi -iso la ted annul a te lamellae were prepared from single newt oocy tes (Triturus alpestris) by a modified Call a n-T omlin technique. Such preparations were examined with the electron mi croscope, and the negative sta ining a ppearance of th e a nnulate lamellae is described . The annul a te lamellae can be de tected either adhering to the nuclear envelope or being detached from it. Sometimes they a re obse rved to be connected with slender tubular-like structures interpreted as pa rts of the endoplasmic reti culum. The results obta ined from negativ e sta ining a re combined with those from sections. Especially, the structural data on th e a nnula te lamellae and the nuclear envelope of the very same cell were compa red . Evidence is presented th a t in the oocytes studied the two kinds of porous cisternae, n amely a nnul a te lamellae and nuclear envelope, a re markedly distinguished in that the annul a te lamellae ex hibit a much higher pore frequency (generally about twice tha t found for the corresponding nuclear envelope) and have al so a rela tive pore area occupying as much as 32 \% to 55 \% of th e cistern al surface (compa red with 13 \% to 22 \% in the nuclear envelopes). T he pore di ame ter a nd all other ultras tructural details of the pore complexes, however, a re equi valent in both kinds of porous cisternae. Like the annuli of the nuclear pore complexes of various a nimal and pl ant cells, the a nnuli of the a nnula te lamellae pores reveal al so an eightfold symmetry of their subunits in negatively stained as well as in ectioned ma teria l. Furthermore, th e a nnul a te lamellae a re shown to be a site of activity of the Mg-Na-Kstimul a ted ATPase.}, language = {en} } @article{RoseSzopaHanetal.1988, author = {Rose, Kathleen M. and Szopa, Jan and Han, Fu-Sheng and Cheng, Yung-Chi and Richter, Arndt and Scheer, Ulrich}, title = {Association of DNA topoisomerase I and RNA polymerase I: A possible role for topoisomerase I in ribosomal gene transcription}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33901}, year = {1988}, abstract = {RNA polymerase I preparations purified from a rat hepatoma contained DNA topoisomerase activity. The DNA topoisomerase associated with the polymerase had an Mr of 110000, required Mg2+ but not ATP, and was recognized by anti-topoisomerase I antibodies. When added to RNA polymerase I preparations containing topoisomerase activity, anti-topoisomerase I antibodies were able to inhibit the DNA relaxing activity of the preparation as well as RNA synthesis in vitro. RNA polymerase II prepared by analogous procedures did not contain topoisomerase activity and was not recognized by the antibodies. The topoisomerase I: polymerase I complex was reversibly dissociated by column chromatography on Sephacryl S200 in the presence of 0.25 M (NH4hS04. Topoisomerase I was immunolocalized in the transcriptionally active ribosomal gene complex containing RNA polymerase I in situ. These data indicate that topoisomerase I and RNA polymerase I are tightly complexed both in vivo and in vitro, and suggest a role for DNA topoisomerase I in the transcription of ribosomal genes.}, language = {en} } @article{RoeschAtzmuellerSchaacketal.1994, author = {R{\"o}sch, M. and Atzm{\"u}ller, R. and Schaack, G. and Becker, Charles R.}, title = {Resonant Raman scattering in a zero-gap semiconductor: Interference effects and deformation potentials at the E\(_1\) and E\(_1\) + \(\Delta_1\) gaps of HgTe}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-38062}, year = {1994}, abstract = {No abstract available}, language = {en} } @article{KrausBeckerScholletal.1993, author = {Kraus, M. M. and Becker, Charles R. and Scholl, S. and Wu, Y. S. and Yuan, S. and Landwehr, G.}, title = {Infrared photoluminescence on molecular beam epitaxially grown Hg\(_{1-x}\)Cd\(_x\)Te layers}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-38053}, year = {1993}, abstract = {No abstract available}, language = {en} } @article{HeBeckerBicknellTassiusetal.1993, author = {He, L. and Becker, Charles R. and Bicknell-Tassius, R. N. and Scholl, S. and Landwehr, G.}, title = {Molecular beam epitaxial growth of (100) Hg\(_{0.8}\)Cd\(_{0.2}\)Te on Cd\(_{0.96}\)Zn\(_{0.04}\)Te}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-38044}, year = {1993}, abstract = {No abstract available}, language = {en} } @article{WuBeckerWaagetal.1993, author = {Wu, Y. S. and Becker, Charles R. and Waag, A. and von Schierstedt, K. and Bicknell-Tassius, R. N. and Landwehr, G.}, title = {Surface sublimation of zinc blende CdTe}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37829}, year = {1993}, abstract = {The surface sublimation of Cd and Te atoms from the zinc blende (111)A CdTe surface has been investigated in detail by reflection high energy electron diffraction and x-ray photoelectron spectroscopy. These experiments verify that Te is much easier to evaporate than Cd. The experimental value for the Te activation energy from a Te stabilized (111)A CdTe surface is 1.41 ±0.1O eV, which is apparently inconsistent with recent theoretical results.}, language = {en} } @article{WuBeckerWaagetal.1992, author = {Wu, Y. S. and Becker, Charles R. and Waag, A. and Bicknell-Tassius, R. N. and Landwehr, G.}, title = {Thermal effects on (100) CdZnTe substrates as studied by x-ray photoelectron spectroscopy and reflection high energy electron diffraction}, issn = {0003-6951}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37801}, year = {1992}, abstract = {The influence of different CdZnTe substrate treatments prior to II-VI molecular beam epitaxial growth on surface stoichiometry, oxygen, and carbon contamination has been studied using x-ray photoelectron spectroscopy and reflection high energy electron diffraction. Heating the substrate at 300 °C can eliminate oxygen contamination, but cannot completely remove carbon from the surface. Heating at higher temperatures decreases the carbon contamination only slightly, while increasing the Zn-Cd ratio on the surface considerably. The magnitude of the latter effect is surprising and is crucial when one is using lattice matched CdZnTe (Zn 4\%) substrates.}, language = {en} } @article{WuBeckerWaagetal.1991, author = {Wu, Y.S. and Becker, Charles R. and Waag, A. and Kraus, M. M. and Bicknell-Tassius, R. N. and Landwehr, G.}, title = {Correlation of the Cd-to-Te ratio on CdTe surfaces with the surface structure}, isbn = {0163-1829}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37789}, year = {1991}, abstract = {We report here that reconstruction on (100), (1lIlA, and (1l1lB CdTe surfaces is either C(2X2), (2X2), and (l X I) or (2X I), (l X I), and (l X I) when they are Cd or Te stabilized, respectively. There is a mixed region between Cd and Te stabilization in which the reflected high-energy electron-diffraction (RHEED) patterns contain characteristics of both Cd- and Te-stabilized surfaces. We have also found that the Cd-to-Te ratio of the x-ray photoelectron intensities of their 3d\(_{3/ 2}\) core levels is about 20\% larger for a Cd-stabilized (1lIlA, (1lIlB, or (100) CdTe surface than for a Te-stabilized one. According to a simple model calculation, which was normalized by means of the photoelectron intensity ratio of a Cd-stabilized (lll)A and aTe-stabilized (1l1lB CdTe surface, the experimental data for CdTe surfaces can be explained by a linear dependence of the photoelectron-intensity ratio on the fraction of Cd in the uppermost monatomic layer. This surface composition can be correlated with the surface structure, i.e., the corresponding RHEED patterns. This correlation can in turn be employed to determine Te and Cd evaporation rates. The Te reevaporation rate is increasingly slower for the Te-stabilized (Ill) A, (l1l)B, and (100) surfaces, while the opposite is true for Cd from Cd-stabilized (Ill) A and (Ill)B surfaces. In addition, Te is much more easily evaporated from all the investigated surfaces than is Cd, if the substrate is kept at normal molecular-beam-epitaxy growth temperatures ranging from 2oo·C to 300 ·C.}, subject = {Festk{\"o}rperphysik}, language = {en} } @article{QiuHeLietal.1993, author = {Qiu, Yueming and He, Li and Li, Jie and Yuan, Shixin and Becker, Charles R. and Landwehr, G.}, title = {Infrared photoconductor fabricated with HgTe/CdTe superlattice grown by molecular beam epitaxy}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37772}, year = {1993}, abstract = {An infrared photoconductor fabricated with a HgTe/CdTe superlattice grown on a GaAs substrate by molecular beam epitaxy is described here for the first time. The growth procedure, device fabrication, and measurement results are described. The results show that the device has relatively high uniformity and 1000 K black-body detectivity 2.4 X 10\(^9\) cm Hz\(^{1/2}\) W\(^{-1}\) . The photoconductivity decay method was used for determining carrier lifetime of the HgTe/CdTe superlattice, the measured lifetime is 12\(\mu\)s at 77 K, which is the longest lifetime ever reported for HgTe/CdTe superlattices and we believe that the increase of lifetime is mainly due to the reduction of dimensions.}, language = {en} } @article{ToenniesBacherForcheletal.1994, author = {T{\"o}nnies, D. and Bacher, G. and Forchel, Alfred and Waag, A. and Litz, Th. and Hommel, D. and Becker, Charles R. and Landwehr, G. and Heuken, M. and Scholl, M.}, title = {Optical study of interdiffusion in CdTe and ZnSe based quantum wells}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37750}, year = {1994}, abstract = {No abstract available}, language = {en} } @article{BeckerHeRegnetetal.1993, author = {Becker, Charles R. and He, L. and Regnet, M. M. and Kraus, M.M. and Wu, Y. S. and Landwehr, G. and Zhang, X. F. and Zhang, H.}, title = {The growth and structure of short period (001) Hg\(_{1-x}\)Cd\(_x\)Te-HgTe superlattices}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37858}, year = {1993}, abstract = {Molecular beam epitaxially grown short period (001) Hg\(_{1_x}\)Cd\(_x\)Te-HgTe superlattices have been systematically investigated. Several narrow well widths were chosen, e.g., 30, 35 and 40 {\AA}, and the barrier widths were varied between 24 and 90 {\AA} for a particular well width. Both the well width and the total period were determined directly by means of x-ray diffraction. The well width was determined by exploiting the high reflectivity from HgTe and the low reflectivity from CdTe for the (002) Bragg reflection. Knowing the well and barrier widths we have been able to set an upper limit on the average Cd concentration of the barriers, \(\overline x_b\), by annealing several superlattices and then measuring the composition of the resulting alloy. \(\overline x_b\) was shown to decrease exponentially with decreasing barrier width. The structure of a very short period superlattice, i.e., 31.4 {\AA}, was also investigated by transmission electron microscopy, corroborating the x-ray diffraction results.}, language = {en} } @article{WuBeckerWaagetal.1993, author = {Wu, Y. S. and Becker, Charles R. and Waag, A. and Schmiedl, R. and Einfeldt, S. and Landwehr, G.}, title = {Oxygen on the (100) CdTe surface}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37869}, year = {1993}, abstract = {We have investigated oxygen on CdTe substrates by means of x-ray photoelectron spectroscopy (XPS) and reflection high-energy electron diffraction (RHEED). A Te oxide layer that was at least 15 A thick was found on the surface of as-delivered CdTe substrates that were mechanically polished. This oxide is not easily evaporated at temperatures lower than 350°C. Furthermore, heating in air, which further oxidizes the CdTe layer, should be avoided. Etching with HCI acid (15\% HCl) for at least 20 s and then rinsing with de-ionized water reduces the Te oxide layer on the surface down to 4\% of a monoatomic layer. However, according to XPS measurements of the 0 Is peak, 20\%-30\% of a monoatomic layer of oxygen remains on the surface, which can be eliminated by heating at temperatures ranging between 300 and 340 cC. The RHEED patterns for a molecular beam epitaxially (MBE)-grown CdTe film on a (lOO) CdTe substrate with approximately one monoatomic layer of oxidized Te on the surface lose the characteristics of the normal RHEED pattems for a MBE-grown CdTe film on an oxygen-free CdTe substrate.}, language = {en} } @article{KrausRegnetBeckeretal.1992, author = {Kraus, M. M. and Regnet, M. M. and Becker, Charles R. and Bicknell-Tassius, R. N. and Landwehr, G.}, title = {Comparison of band structure calculations and photoluminescence experiments on HgTe/CdTe superlattices grown by molecular beam epitaxy}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37878}, year = {1992}, abstract = {We have grown HgTe/CdTe superlattices by molecular beam epitaxy; barrier thicknesses were in the range from 15 to 91 {\AA} and the well thickness was maintained at a constant value of 30 {\AA}. The infrared photoluminescence was investigated by means of Fourier transform infrared spectroscopy in the temperature range from 4.2 to 300 K. All superlattices showed pronounced photoluminescence at temperatures up to 300 K. To gain more detailed insight into the band structure of the HgTe/CdTe superlattices, band structure calculations were performed. The concept of the envelope function approximation was followed. Employing the transfer matrix method, the calculations were completed taking into account an eight band k·p model. An important parameter in these calculations is the natural valence band offset (VBO) between the well and barrier materials. As a general trend, the value for the direct gap decreases with increasing VBO. The experimentally determined energies of the band gap are in reasonable agreement with the values obtained by the theoretical calculations. A comparison between theory and experiment shows that the observed transition energies are closer to calculations employing a large offset (350 meV) as opposed to a small VBO (40 meV).}, language = {en} } @article{HerrmannHappMoellmannetal.1993, author = {Herrmann, K. H. and Happ, M. and M{\"o}llmann, K.-P. and Tomm, J. W. and Becker, Charles R. and Kraus, M. M. and Yuan, S. and Landwehr, G.}, title = {A new model for the absorption coefficient of narrow gap (Hg,Cd)Te that simultaneously considers band tails and band filling}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37894}, year = {1993}, abstract = {A semiempirical model is presented that correlates the broadening of the absorption edge with both transitions below the energy gap and with transitions by the Kane band model. This model correctly fits both the absorption and luminescence spectra of narrow-gap (Hg,Cd)Te samples that have been grown by the traveling heater method as well as by molecular-beam epitaxy. The accuracy of the band-gap determination is enhanced by this model.}, language = {en} } @article{WaagHeinkeScholletal.1993, author = {Waag, A. and Heinke, H. and Scholl, S. and Becker, Charles R. and Landwehr, G.}, title = {Growth of MgTe and Cd\(_{1-x}\)Mg\(_x\)Te thin films by molecular beam epitaxy}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37917}, year = {1993}, abstract = {We report on the growth of the compound semiconductor MgTe as weil as the ternary alloy Cd\(_{l-x}\)Mg, Te by molecular hcam cpitaxy. This is to our knowkdgc thc first time that this material has heen grown by any epitaxial technique. Bulk MgTe, which is hygroscopic, has a band gap of 3.0 eV and crystallizcs usually in thc wurtzite structure. Pseudomorphic films were grown on zincblende Cd Te suhstrates for a MgTe thickness helow a critical layer thickncss of approximately 500 nm. In addition, Cd\(_{l_x}\),Mg\(_x\)Te epilayers were grown with a Mg concentration between 0 and 68\%, which corresponds to a band gap betwcen 1.5 and 2.5 eV at room temperature. The crystalline quality of thc layers is comparabk to CdTc thin films as long as they are fully strained. The lauice constant of zincblende MgTe is slightly smaller than that of CdTe, and the lattice mismatch is as low as O.7\%. In addition highly n-type CdMgTe layers were fabricatcd by hromine doping. The tunability of the band gap as weil as the rather good laUice match with CdTc makes the matcrial interesting for optoelectronic device applications for the entire visible range.}, language = {en} } @article{ShanjiaXinzhangGreineretal.1992, author = {Shanjia, Xu and Xinzhang, Wu and Greiner, P. and Becker, Charles R. and Geick, R.}, title = {Microwave transmission and reflection of stratified lossy dielectric segments partially filled waveguide}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37958}, year = {1992}, abstract = {The microwave transmission and reßection is evaluated for stratified lossy dielectric segments partially filling the rectangular waveguide by the method which combines the multimode network theory with the rigorous mode matching procedure. As an example, we investigate in detail the microwave scattering properties of II-VI-epitaxial layer on a lossy dielectric substrate inserted in the rectangular waveguide. The experimental data verify the accuracy and the effectiveness of the present method. Extensive numerical results are presented to establish useful guidelines for the contactless microwave measurement of the conductivity of the epitaxiallayer.}, language = {en} } @article{ShanjiaXinzhangBoegeetal.1993, author = {Shanjia, Xu and Xinzhang, Wu and Boege, P. and Sch{\"a}fer, H. and Becker, Charles R. and Geick, R.}, title = {Scattering characteristics of 3-D discontinuity consisting of semiconductor sample filled in waveguide with gaps}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37964}, year = {1993}, abstract = {No abstract available}, language = {en} } @article{HeBeckerBicknellTassiusetal.1993, author = {He, L. and Becker, Charles R. and Bicknell-Tassius, R. N. and Scholl, S. and Landwehr, G.}, title = {Molecular beam epitaxial growth and evaluation of intrinsic and extrinsically doped (100) Hg\(_{0.8}\)Cd\(_{0.2}\)Te on (100) Cd\(_{0.96}\)Zn\(_{0.04}\)Te}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37885}, year = {1993}, abstract = {No abstract available.}, language = {en} } @article{SchikoraHausleitnerEinfeldtetal.1994, author = {Schikora, D. and Hausleitner, H. and Einfeldt, S. and Becker, Charles R. and Widmer, T. and Giftige, C. and Lischka, K. and von Ortenburg, M. and Landwehr, G.}, title = {Epitaxial overgrowth of II-VI compounds on patterned substrates}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37985}, year = {1994}, abstract = {The selected area epitaxial overgrowth of narrow gap HgTe as well as wide gap CdTe and ZnTe on CdTe/GaAs substrates, which had been structured by dry etching techniques, has been investigated. A plasma etching process using a barrel reactor with CH\(_4\)-CH\(_2\) gases has been employed to prepare stripes with a width of about 1 μm with anisotropic as well as isotropic etching profiles. It has been found, that the selected area HgTe overgrowth takes place with a high local selectivity to the low index planes of the patterned surface. In contrast, the selected area overgrowth of the wide gap CdTe and ZnTe is controlled by anisotropic growth kinetics provided that the substrate temperature is not lower than 220°C and the starting surface consists of well developed low index crystallographic planes.}, language = {en} } @article{EinfeldtHeinkeBehringeretal.1994, author = {Einfeldt, S. and Heinke, H. and Behringer, M. and Becker, Charles R. and Kurtz, E. and Hommel, D. and Landwehr, G.}, title = {The growth of HgSe by molecular beam epitaxy for ohmic contacts to p-ZnSe}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-38001}, year = {1994}, abstract = {The structural properties of HgSe grown by molecular beam epitaxy (MBE) are investigated for different lattice mismatches to the substrate and various growth conditions. The growth rate is shown to depend strongly on the growth temperature above lOO°C as well as on the Hg/Se flux ratio. It has been found that the crystalline perfection and the electrical properties are mainly determined by the layer thickness, especially for the growth on highly lattice mismatched substrates. Changes in the surface morphology are related to growth parameters. Differences between the electrical behavior of MBE-grown and bulk HgSe are discussed. The electrical properties of HgSe contacts on p-ZnSe are investigated as a function of different annealing procedures.}, language = {en} } @article{WuBeckerWaagetal.1993, author = {Wu, Y. S. and Becker, Charles R. and Waag, A. and Bicknell-Tassius, R. N. and Landwehr, G.}, title = {Removal of oxygen and reduction of carbon contamination on (100) Cd\(_{0.96}\)Zn\(_{0.04}\)Te}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-38014}, year = {1993}, abstract = {No abstract available}, language = {en} } @article{ZhangBeckerZhangetal.1994, author = {Zhang, X. F. and Becker, Charles R. and Zhang, H. and He, L. and Landwehr, G.}, title = {Investigation of a short period (001) HgTe-Hg\(_{0.6}\)Cd\(_{0.4}\)Te superlattice by transmission electron microscopy}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-38029}, year = {1994}, abstract = {No abstract available}, language = {en} } @article{BeckerMartin1972, author = {Becker, Charles R. and Martin, T. P.}, title = {Infrared absorption by Impurity-pair resonant modes in NaCl:F}, isbn = {1098-0121}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37840}, year = {1972}, abstract = {New resonant-mode infrared absorption lines have been observed in NaCl with high concentrations of fluorine impurities. The quadratic concentration dependence of the strength of these lines indicates that they are due to pairs of fluorine impurities. At the resonant frequencies, the motion of some host ions appears to be as important as the motion of the impurities themselves.}, subject = {Festk{\"o}rperphysik}, language = {en} } @article{BeckerNath1970, author = {Becker, Charles R. and Nath, G.}, title = {Optical properties of LiIO\(_3\) in the far infrared}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37905}, year = {1970}, abstract = {No abstract available}, language = {en} } @article{RoederSteinleinSchmidetal.1993, author = {R{\"o}der, G. and Steinlein, C. and Schmid, M. and Linsenmair, Karl Eduard}, title = {Karyotype and chromosome banding in the Turkish desert woodlouse Desertellio elongatus (Crustacea, Isopoda, Oniscidea)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30989}, year = {1993}, abstract = {The karyotype of D. elongatus was investigated by means of C-banding, silver staining, and mithramycinand quinacrine fluorescent staining. The diploid chromosome number is 2n = 50. C-banding shows pericentromerically localized constitutive heterochromatin in every chromosome. Two of the chromosome pairs carry two telomeric nucleolus organizer regions each. No heteromorphic sex chromosomes were found.}, language = {en} } @article{Linsenmair1984, author = {Linsenmair, Karl Eduard}, title = {Comparative studies on the social behaviour of the desert isopod Hemilepistus reaumuri and of a Porcellio species}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30846}, year = {1984}, abstract = {Behavioural adaptations have made the desert isopod Hemilepistus reaumuri the most successful herbivore and detritivore of the macrofauna of many arid areas in North Africa and Asia Minor. For survival and reproduction Hemilepistus is dependent on burrows. New burrows can only be dug during spring. With the time-consuming digging of a burrow, Hemilepistus has only made the first step towards solving its ecological problems. The burrows are vital and have to be continuously defended against competitors. This requirement is met by co-operation of individuals within the framework of a highly developed social behaviour. In spring adults form monogamous pairs in which partners recognize each other individually and later form, with their progeny, strictly closed family communities. Hemilepistus is compared with a Porcellio' sp. which has developed, convergently, a social behaviour which resembles that of Hemilepistus in many respects, but differs essentially in some aspects, partly reflecting differences in ecological requirements. This and a few other Porcellio species demonstrate some possible steps in the evolution of the social behaviour of Hemilepistus. The female Hemilepistus is-in contrast to Porcellio sp. - semelparous and the selective advantages of monogamy in its environment are not difficult to recognize. This chapter discusses how this mating system could have evolved and especially why monogamous behaviour is also the best method for the Hemilepistus male to maximize its reproductive success. The cohesion of pairs and of family communities in Hemilepistus is based on a highly developed chemical communication system. Individual- and family-specific badges owe their specificity to genetically determined discriminating substances. The nature of the badges raises a series of questions: e.g. since alien badges release aggression, how do parents avoid cannibalizing their young? Similar problems arise from the fact that family badges are mixtures of chemical compounds of very low volatility with the consequence that they can only be transferred by direct contact and that during moulting all substances are lost which an individual does not produce itself. It is shown that in solving these problems inhibiting properties (presumably substances) and learning play a dominant role.}, language = {en} } @article{Linsenmair1985, author = {Linsenmair, Karl Eduard}, title = {Individual and family recognition in subsocial arthropods, in particular in the desert isopod Hemilepistus reaumuri}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33957}, year = {1985}, abstract = {Individual recogmtlon in the non-eusocial arthropods is, according to our present knowledge, predominantly found in the frame of permanent or temporary monogamy. In some cases, e. g. in stomatopods and possibly other marine crustaceans too, individual recognition may serve to allow identification of (i) individuals within dominance hierarchies or (ii) neighbours in territorial species thus helping to avoid the repetition of unnecessary and costly fights. Kin recognition is experimentally proven only in some isopod species (genera Hemilepistus and Porcel/io) and in the primitive cockroach (termite?) Cryptocercus. The «signatures» or «discriminators» used in the arthropods are chemical. It is assumed that the identifying substances are mainly genetically determined and in this paper I shall discuss possible evolutionary origins. The main part of this account is devoted to the presentation of some aspects of the highly developed individual and kin identification and recognition system in the desert isopod Hemilepistus reaumuri - a pure monogamous species in which pairs together with their progeny form strictly exclusive family units. Amongst other things problems of (i) mate choice, (ii) learning to recognize a partner, (iii) avoiding the un adaptive familiarization with aliens are treated. Monogamy under present conditions is for both sexes the only suitable way of maximizing reproductive success; an extremely strong selection pressure must act against every attempt to abandon monogamy under the given ecological conditions. The family «badges» which are certainly always blends of different discriminator substances are extremely variable. This variability is mainly due to genetical differences and is not environmentally caused. It is to be expected that intra-family variabiliry exists in respect of the production of discriminator substances. Since the common badge of a family is the result of exchanging and mixing individual substances, and since the chemical nature of these discriminators requires direct body contacts in order to acquire those substances which an individual does not produce itself, problems must arise with molting. These difficulties do indeed exist and they are aggravated by the fact that individuals may produce substances which do not show up in the common family badge. An efficient learning capability on the one hand and the use of inhibiting properties of newly molted isopods help to solve these problems. In the final discussion three questions are posed and - partly at least - answered; (i) why are families so strictly exclusive, (ii) how many discriminator substances have to be produced to provide a variability allowing families to remain exclusive under extreme conditions of very high population densities, (iii) what is the structure of the family badge and what does an individual have to learn apart from the badge in order not to mistake a family member for an alien or vice versa.}, language = {en} } @article{GrafeLinsenmair1989, author = {Grafe, U. and Linsenmair, Karl Eduard}, title = {Protogynous sex change in the Reed Frog: Hyperolius viridiflavus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30990}, year = {1989}, abstract = {Observations on captive reed frogs Hyperolius viridijlavus ommatostictus showed that seven out of 24 females changed into males. Sex change occurred without any hormone treatment and resulted in completely functional males. The adaptive value is discussed in terms of maximizing life-time reproductive success. Hyperolius r. ommatostictus is the first amphibian known to show functional sex reversal.}, language = {en} } @article{SchmuckGeiseLinsenmair1994, author = {Schmuck, R. and Geise, W. and Linsenmair, Karl Eduard}, title = {Life cycle strategies and physiological adjustments of Reedfrog Tadpoles (Amphibia, Anura, Hyperoliidae) in relation to environmental conditions.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31009}, year = {1994}, abstract = {The relationship between different degrees of intraspecific crowding of reedfrog tadpoles and their physiological responses to a deterioration of the natal pond water quality was examined under laboratory conditions. Tadpoles that were reared at a lower density metamorphosed significantly earlier than those raised at a higher density. As density increases, the average body length at metamorphosis decreases. However, at low tadpole density, a significantly higher diversity of body size classes among freshly metamorphosed froglets was observed than under more crowded conditions. Mortality increased during metamorphic climax and was inversely correlated with the tadpole density. In ephemeral ponds, an accumulation of nitrogenous wastes from metabolic processes and/or a concentration by evaporation in prolonged rainless periods can pose a considerable chemical stress to reedfrog tadpoles. Hyperolius viridiflavus ommatostictus responded to an increasing ammonia concentration with an activity increase of the ornithine cycle (intensified urea synthesis). hi contrast, Hyperolius marmoratus taeniatus exhibited a strong tolerance against high ammonia levels. A deterioration of the natal pond water quality caused H. v. ommatostictus and H. v. nitidulus tadpoles to adjust to harsher climatic conditions at the time of metamorphosis. This physiological preadjustment enabled the froglets to start feeding and growing immediately after metamorphosis even at low air humidity and rare precipitation events. In contrast, froglets that were raised in daily refreshed water exhibited high mortality rates if subjected to identical conditions. As one possible indicator of the actual climatic conditions prevailing in the surrounding terrestrial habitat, fluctuations in the water ammonia level are discussed.}, language = {en} } @article{TackeSchuberthBeckeretal.1982, author = {Tacke, M. and Schuberth, W. and Becker, Charles R. and Haas, L. D.}, title = {The dielectric constant of PbTe at 4.2 K and \(\tilde ν\)=84.15 cm\(^{-1}\), 96.97 cm\(^{-1}\), 103.60 cm\(^{-1}\)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30821}, year = {1982}, abstract = {The dielectric constant of a PbTe epitaxial layer has been measured by surface wave spectroscopy using an optically pumped far-infrared laser and the technique of attenuated total reflection.}, language = {en} }