@article{RethwilmErlweinBaunachetal.1991,
  author    = {Rethwilm, Axel and Erlwein, Otto and Baunach, Gerald and Mauerer, Bernd and ter Meulen, Volker},
  title     = {The transcriptional transactivator of human foamy virus maps to the bel 1 genomic region},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-47342},
  year      = {1991},
  abstract  = {The human foamy virus (HFV) genome possesses three open reading frames (bel I, 2, and 3) located between env and the 3' long terminal repeat. By analogy to other human retroviruses this region was selected as the most Iikely candidate to encode the viral transactivator. ResuIts presented here confirmed this and showed further that a deletion introduced only into the bell open reading frame of a plasmid derived from an infectious molecular clone of HFV abolished transactivation. In contrast, deletions in bel 2 and bel 3 had only minor effects on the ability to transactivate. The role of the bel I genomic region as a transactivator was further investigated by eukaryotic expression of a genome fragment of HFV spanning the bel I open reading frame. A construct expressing bell under control of a heterologous promoter was found to transactivate the HFV long terminal repeat in a dose-dependent fashion. Furthermore, it is shown that the U3 region of the HFV long terminal repeat is sufficient to respond to the HFV transactivator.},
  subject      = {Virologie},
  language  = {en}
}
@article{MaurerSerflingterMeulenetal.1991,
  author    = {Maurer, Bernd and Serfling, Edgar and ter Meulen, Volker and Rethwilm, Axel},
  title     = {Transcription factor AP-1 modulates the activity of the human foamy virus long terminal repeat},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61444},
  year      = {1991},
  abstract  = {The human foamy virus (HFV) contains within the UJ region of its long terminal repeat (L TR) three perfect consensus sequences for the binding of the inducible transcription factor AP-1. Results of DNase I footprint protection and gel retardation assays demonstrated that proteins in extracts of HeLa and BHK-21 cells as weil as bacterially expressed Jun and Fos proteins bind to these AP-1 sites. By conducting transient expression assays using chloramphenicol acetyltransferase plasmids carrying LTR sequences with point-mutated AP-1 sites it was found that the three AP-1 sites contribute to the optimal activity ofthe HFV promoter. It is shown that lnduction of the HFV L TR by 12-O-tetradecanoylphorbol-13-acetate (TPA) and serum factors is mediated through the AP-1 sites.},
  subject      = {Virologie},
  language  = {en}
}