@article{WittbrodtAdamMalitscheketal.1989,
  author    = {Wittbrodt, J. and Adam, D. and Malitschek, B. and Maueler, W. and Raulf, F. and Telling, A. and Robertson, M. and Schartl, Manfred},
  title     = {Novel putative receptor tyrosine kinase encoded by the melanoma-inducing Tu locus in Xiphophorus},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61800},
  year      = {1989},
  abstract  = {No abstract available},
  subject      = {Physiologische Chemie},
  language  = {en}
}
@article{WinotoMorbachUlrichsLeyhausenetal.1989,
  author    = {Winoto-Morbach, Supandi and Ulrichs, Karin and Leyhausen, Gaby and M{\"u}ller-Ruchholtz, Wolfgang},
  title     = {New principle for large-scale preparation of purified human pancreas islets},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45600},
  year      = {1989},
  abstract  = {Because successful human islet transplantation requires large quantities of viable islets that must be separated from the highly immunogenic exocrine tissue and because handpicking is too time-consuming and laborious to be clinically relevant, a new approach for solving this problem has been established in rat models. It is based on the principle that magnetic microspheres (MMSs) coupled to lectins with binding specificity for the exocrine tissue portion are trapped in an electromagnetic field, thus providing effluent islets of a high degree of purity. In this study our aim was to adapt this princip'le to human islet preparations. In this context our prime interest was focused on a lectin suitable for human pancreatic tissue. Of 19 different lectins tested, only 1, Wisteria floribunda agglutinin (WFA), is suitable, as shown by immunofluorescence, MMS-Iectin binding, and magnetic separation},
  subject      = {Immunbiologie},
  language  = {en}
}
@article{WinotoMorbachLeyhausenSchuenkeetal.1989,
  author    = {Winoto-Morbach, S. and Leyhausen, G. and Sch{\"u}nke, M. and Ulrichs, Karin and M{\"u}ller-Buchholtz, W.},
  title     = {Magnetic microspheres (MMS) coupled to selective lectins: a new tool for large-scale extraction and purification of human pancreatic islets},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-44789},
  year      = {1989},
  abstract  = {No abstract available},
  subject      = {Chirurgie},
  language  = {en}
}
@article{Wilhelm1989,
  author    = {Wilhelm, Gernot},
  title     = {Die zweite Tafel der Serie Kagal in Ḫattusa},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-87806},
  year      = {1989},
  abstract  = {no Abstract available},
  subject      = {Kagal},
  language  = {de}
}
@article{WieberBurschkaBauer1989,
  author    = {Wieber, Markus and Burschka, Christian and Bauer, Bernd},
  title     = {Kristallstruktur von Bis(diethyldithiocarbamato)phenylphosphan und Synthese einiger Mono- und Bis(dioganyldithiocarbamato)phosphane},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-57814},
  year      = {1989},
  abstract  = {Durch Umsetzung der jeweiligen Chiaraphosphane mit den entsprechenden Natriumdithiocarbamaten k{\"o}nnen folgende Verbindungen erhalten werden: Verbindungen des Typs RP(S\(_2\)CNR\(_2\)')\(_2\) mit R = CH\(_3\), C\(_6\)H\(_5\); R' = CH\(_3\) , C\(_2\)H\(_5\), CH(CH\(_3\))\(_2\). C\(_6\)H\(_5\) ; Verbindungen des Typs (C\(_6\)H\(_5\)) \(_2\)PS\(_2\)CNR\(_2\) mit R' = CH\(_3\) , CH(CH\(_3\))\(_2\) sowie [(C\(_6\)H\(_5\)))\(_2\)PS\(_2\)CN(CH\(_3\))CH\(_2\)--]\(_2\). Die Kristallstruktur von C\(_6\)H\(_5\)P(S\(_2\)CN(C\(_2\)H\(_5\))\(_2\))\(_2\) zeigt, daß sich der Trend zu schw{\"a}cher ausgepr{\"a}gter zweiz{\"a}hniger Bindungsweise der Dithiocarbamatliganden in der homologen Reihe RE(S\(_2\)CN(C\(_2\)H\(_5\))\(_2\))\(_2\); E = Bi, Sb, As, P f{\"u}r E = P fortsetzt.},
  subject      = {Anorganische Chemie},
  language  = {de}
}
@article{WeigelMeyerSebald1989,
  author    = {Weigel, U. and Meyer, M. and Sebald, Walter},
  title     = {Mutant proteins of human interleukin 2. Renaturation yield, proliferative activity and receptor binding},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62543},
  year      = {1989},
  abstract  = {No abstract available},
  subject      = {Biochemie},
  language  = {en}
}
@article{Wehgartner1989,
  author    = {Wehgartner, Irma},
  title     = {Man leaning on his stick : Zu Bild und Inschrift eines attischen Salbgef{\"a}ßes ; (mit einem Beitrag von G{\"u}nter Neumann)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-70047},
  year      = {1989},
  abstract  = {No abstract available.},
  subject      = {Inschrift},
  language  = {de}
}
@article{WeberSchmidtScheer1989,
  author    = {Weber, Thomas and Schmidt, Erwin and Scheer, Ulrich},
  title     = {Mapping of transcription units on Xenopus laevis lampbrush chromosomes by in situ hybridization with biotin-labeled cDNA probes},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-40763},
  year      = {1989},
  abstract  = {A non-radioactive in situ hybridization method is described for the localization of transcription units of defined genes to lateral loops of Xenopus laevis lampbrush chromosomes. Two Xenopus cONA probes were used encoding the nucleolar protein N038/ B23 and cytokeratin 1(8). Both proteins are known to be synthesized in Xenopus oocytes, and Northern blot analysis revealed the presence of the corresponding mRNAs in different oogenic stages. The probes were enzymatically labeled with biotin-dCTP and hybridized to lampbrush chromosomes. The sites of hybridization were detected either by indirect immunofluorescence microscopy using rabbit antibodies against biotin and fluorescein-conjugated antirabbit IgG or enzymatically using peroxidase-conjugated streptavi din. The probe encoding the nucleolar protein hybridized to two sets of lateral loops on different bivalents, the cytokeratin probe to at least four. Our finding that each probe hybridized to more than one chromosomal locus may reflect the tetraploid nature of the Xenopus laevis genome or results from cross-hybridization to other transcriptionally active members of the N038/ B23-nucleoplasmin or the cytokeratin-Iamin gene families. The method described should facilitate further in situ hybridization studies with appropriate genomic clones in order to map specific DNA sequences to defined loop regions and to come to a better understanding of the relationship between loop organization and gene transcription unit.},
  subject      = {Cytologie},
  language  = {en}
}
@article{WaelbroeckTastenoyCamusetal.1989,
  author    = {Waelbroeck, M. and Tastenoy, M. and Camus, J. and Christophe, J. and Strohmann, C. and Linoh, H. and Zilch, H. and Tacke, Reinhold and Mutschler, E. and Lambrecht, G.},
  title     = {Binding and functional properties of antimuscarinics of the hexocyclium/sila-hexocyclium and hexahydro-diphenidol/hexahydro-sila-diphenidol type to muscarinic receptor subtypes},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63944},
  year      = {1989},
  abstract  = {l In an attempt to assess the structural requirements for the musearlnie receptor selectivity of hexahydro-diphenidol (hexahydro-difenidol) and hexahydro-sila-diphenidol (hexahydro-sila-difenidol), a serles of structurally related C/Si pairs were investigated, along with atropine, pirenzepine and methoctramine, for their binding affinities in NB-OK 1 cells as well as in rat heart and pancreas. 2 The action of these antagonists at musearlnie receptors mediating negative inotropic responses in guinea-pig atrla and ileal contractions has also been assessed. 3 Antagonist binding data indicated that NB-OK 1 cells (M\(_1\) type) as weil as rat heart (cardiac type) and pancreas (glandularjsmooth muscle type) possess different muscarinic receptor subtypes. 4 A highly significant correlation was found between the binding affinities of the antagonists to muscarinic receptors in rat heart and pancreas, respectively, and the affinities to muscarinic receptors in guinea-pig atria and ileum. This implies that the musearlnie binding sites in rat heart and the receptors in guinea-pig atrla are essentially similar, but different from those in pancreas and ileum. 5 The antimuscarinic potency of hexahydro-diphenidol and hexahydro-sila-diphenidol at the three subtypes was inftuenced differently by structural modifications (e.g. quaternization). Different selectivity profiles for the antagonists were obtained, which makes these compounds useful tools to investigate further muscarinic receptor heterogeneity. lndeed, the tertiary analogues hexahydrodiphenidol (HHD) and hexahydro-sila-diphenidol (HHSiD) bad an M\(_1\) = glandularjsmooth muscle > cardiac selectivity profile, whereas the quaternary analogues HHD methiodide and HHSiD methiodide were M\(_1\) preferring (M\(_1\) > glandularjsmooth muscle, cardiac).},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{VonGaudeckerUlrichsMuellerRuchholtz1989,
  author    = {Von Gaudecker, Brita and Ulrichs, Karin and M{\"u}ller-Ruchholtz, Wolfgang},
  title     = {Immunoelectron microscopic localization of MHC structures in isolated pancreatic rat islets},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45596},
  year      = {1989},
  abstract  = {An immunogold-silver enhancement technique, which combines effective labeling of viable isolated islets with the ultrastructural resolution of cytological details, was applied in electron microscopy to identify major histocompatibility complex (MHC) structures on islet cells. Incubation of freshly isolated islets from CAP (RT1C) and LEW (RT1') rats with OX18, an MHC class I antibody, showed strong positive reactivity in macrophages and/or dendritic-like cells (M0-DCs) and vascular endothelial cells (VEs) and a comparatively weaker reactivity in endocrine a-, p-, and 8-ce"s. With MHC class" antibody OX6 (anti-I-A), M0-DCs were strongly labeled in both rat strains on the surface and on internal structures. Three of five particularly high titered batches of OX6 revealed MHC class" expression on VE and p-ce"s. Four days of in vitro culture in combination with a high concentration of glucose and interferon-'Y induced strong enhancement of MHC class I structures and, to a lesser extent, class " structures on p-ce"s.},
  subject      = {Immunbiologie},
  language  = {en}
}
@article{UlrichsMuellerRuchholtz1989,
  author    = {Ulrichs, Karin and M{\"u}ller-Ruchholtz, W.},
  title     = {Significant manipulative procedures to reduce immunogenicity of human islet allografts},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-45155},
  year      = {1989},
  abstract  = {No abstract available},
  subject      = {Chirurgie},
  language  = {en}
}
@article{TsfasmanNesmeyanovaGorboulevetal.1989,
  author    = {Tsfasman, I. M. and Nesmeyanova, M. A. and Gorboulev, Valentin G. and Rubtsov, P. M. and Skryabin, K. G.},
  title     = {Biosynthesis and secretion of bovine growth hormone in Escherichia coli under the control of the secretory vector containing a promoter and signal sequence of alkaline phosphatase gene},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46932},
  year      = {1989},
  abstract  = {A recombinant plasmid was constructed containing the gene for bovine growth hormone joinea with the regulatory region and the region coding the signal sequence of the Escherichia coli alkaline phosphatase gene. In conditions of phosphorus starvation, which c~s derepression of alkaline phosphatase, expression was shown of the gene for bovine growth hormone, in addition to partial processing and secretion of protein into periplasm.},
  language  = {en}
}
@article{TiuDavernGarciaetal.1989,
  author    = {Tiu, W. U. and Davern, K. M. and Garcia, E. G. and Moll, Heidrun and Mitchell, Graham F.},
  title     = {Monoclonal antibodies reacting with Schistosoma japonicum eggs and their target epitopes},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30916},
  year      = {1989},
  abstract  = {Ten monoclonal antibodies (McAbs) raised to Schistosoma japonicum eggs could be assigned using several serological and immunochemical techniques to 3 groups. The McAbs, termed A, B and C-McAbs, apparently recognize carbohydrate epitopes that can be located on the same antigen molecule. The antibodies, generally of IgM isotype, are idiotypically related. They are distinct from another IgM McAb (Group D-McAb) the carbohydrate target epitope of which can also be associated with the epitopes of A. B and C-McAbs. The McAbs produce large vacuolated bleb reactions in the circumoval precipitin test (COPT) and target epitopes have different representations in various life cycle stages such as immature and mature eggs, male and female worms (including S. mansoni). Antigens affinity purified on columns containing A, B, C and D-McAbs stimulate proliferation of T cells from egg-sensitized mice and elicit DTH reactions in such mice. This raises the possibility that the target antigens of these carbohydrate-reactive monoclonal antibodies are immunopathologic and involved in egg-induced granuloma formation.},
  language  = {en}
}
@article{TawfikSchlieperKlotzKreyeetal.1989,
  author    = {Tawfik-Schlieper, H. and Klotz, Karl-Norbert and Kreye, V. A. W. and Schwabe, U.},
  title     = {Characterization of the K\(^+\)-channel-coupled adenosine receptor in guinea pig atria},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60333},
  year      = {1989},
  abstract  = {In the present work we studied the pharmacological profile of adenosine receptors in guinea pig atria by investigating the effect of different adenosine analogues on 86Rb + -efflux from isolated left atria and on binding of the antagonist radioligand 8-cyclopentyl-1 ,3-[\(^3\)H]dipropylxanthine ([\(^3\)H]DPCPX) to atrial membrane preparations. The rate of \8^{86}\)Rb\(^+\) -effiux was increased twofold by the maximally effective concentrations of adenosine receptor agonists. The EC50-values for 2-chloro-N\(^6\)-cyclopentyladenosine (CCPA), R-N\(^6\)-phenylisopropyladenosine (R-PIA), 5'-Nethylcarboxamidoadenosine (NECA), and S-N\(^6\)-phenylisopropyladenosine (S-PIA) were 0.10, 0.14, 0.24 and 12.9 \(\mu\)M, respectively. DPCPX shifted the R-PIA concentration-response curve to the right in a concentration-dependent manner with a K\(_B\)-value of 8.1 nM, indicating competitive antagonism. [\(^3\)H]DPCPX showed a saturable binding to atrial membranes with a Bmax·value of 227 fmol/mg protein and a K\(_D\)-value of 1.3 nM. Competition experiments showed a similar potency for the three agonists CCPA, R-PIA and NECA. S-PIA is 200 times less potent than R-PIA. Our results suggest that the K\(^+\) channel-coupled adenosine receptor in guinea pig atria is of an A\(_1\) subtype.},
  subject      = {Toxikologie},
  language  = {en}
}
@article{TackeStrohmannSargeetal.1989,
  author    = {Tacke, Reinhold and Strohmann, C. and Sarge, S. and Cammenga, H. K. and Schomburg, D. and Mutschler, E. and Lambrecht, G.},
  title     = {Darstellung und Eigenschaften der Enantiomere des selektiven Antimuscarinikums 1-Cyclohexyl-1-phenyl-4-piperidino-1-butanol (Hexahydro-Difenidol)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63950},
  year      = {1989},
  abstract  = {No abstract available},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{TackeRafeinerStrohmannetal.1989,
  author    = {Tacke, Reinhold and Rafeiner, K. and Strohmann, C. and Mutschler, E. and Lambrecht, G.},
  title     = {Synthesis of the selective antimuscarinic agent 4-{[cyclohexylhydroxy(2-methoxyphenyl)silyl]methyl}-1,1-dimethylpiperazinium methyl sulfate (o-methoxy-sila-hexocyclium methyl sulfate)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63930},
  year      = {1989},
  abstract  = {The synthesis of the potent and highly selective silicon-containing antimuscarinic agent o-methoxysila- hexocyclium methyl sulfate and its corresponding tertiary amine (isolated as the dihydrochloride) is described. The quarternary compound is an omethoxy derivative of sila-hexocyclium methyl sulfate, which represents one of the tools currently used in experimental pharmacology for the subclassification of muscarinic receptors. The omethoxy derivative, the pharmacological profile of which differs substantially from tbat of the nonmethoxy compound, is also recommended as a tool for the investigation of muscarinic receptor heterogeneity.},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{TackeLinohRafeineretal.1989,
  author    = {Tacke, Reinhold and Linoh, H. and Rafeiner, K. and Lambrecht, G. and Mutschler, E.},
  title     = {Synthese und Eigenschaften des selektiven Antimuscarinikums Sila-Hexocyclium-methylsulfat},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63921},
  year      = {1989},
  abstract  = {Sila-Hexocyclium-methylsulfat (7b), ein Silicium-Analogon des therapeutisch eingesetzten Antimuscarinileums Hexocyclium-methylsulfat (7a), wurde durch eine sechsstufige Synthese - ausgehend von (CH\(_3\)0)\(_3\)SiCH\(_2\)Cl - dargestellt (Gesamtausbeute 16\%). Außerdem wurden die hiervon abzuleitende freie Base 9b (f{\"u}nfstufige Synthese, ausgehend von (CH\(_3\)0)\(_3\)SiCH\(_2\)O; Gesamtausbeute 29\%) und das strukturverwandte (Aminomethyl)silanol 13 (dreistufige Synthese, ausgehend von cyclo-C\(_6\)H\(_{11}\)(C\(_6\)H\(_5\))Si(OCH\(_3\))CH\(_2\)Cl, Gesamtausbeute 46\$) synthetisiert. 7b ist ein hochwirksames und selektives Antimuscarinikum, das in der experimentellen Pharmakologie aufgrund seines bemerkenswerten Selektivit{\"a}tsprofils zur Klassifizierung von Subtypen muscarinischer Rezeptoren eingesetzt wird.},
  subject      = {Anorganische Chemie},
  language  = {de}
}
@article{TackeHengelsbergZilchetal.1989,
  author    = {Tacke, Reinhold and Hengelsberg, H. and Zilch, H. and Stumpf, B.},
  title     = {Enantioselective microbial reduction of 1,1-dimethyl-1-sila-cyclohexan-2-one with growing cells of the yeast Kloeckera corticis (ATCC 20109)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-64010},
  year      = {1989},
  abstract  = {(R)-1,1-Dimethyl-1-sila-cyclohexan-2-ol [(R)-2] was prepared by enantioselective microbial reduction of 1,1-dimethyl-1-sila-cyclohexan-2-one (1) with growing cells of the yeast Kloeckera corticis (ATCC 20109). At a substrate concentration of 0.5 g/1 (temperature 27° C, incubation time 16 h), (R}-2 was obtained on a preparative scale in 60\% yield and with an enantiomeric purity of 92\% ee. Repeated recrystallization of the biotransformation product from n-hexane raised the enantiomeric purity to 99\% ee.},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{StoeckliLottspeichSendtneretal.1989,
  author    = {St{\"o}ckli, K. A. and Lottspeich, F. and Sendtner, Michael and Masiakowski, P. and Carroll, Patrick and G{\"o}tz, Rudolf and Lindholm, D. and Thoenen, Hans},
  title     = {Molecular cloning, expression and regional distribution of rat ciliary neurotrophic factor},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34229},
  year      = {1989},
  abstract  = {CILIARY neurotrophic factor (CNTF) was originally characterized as a survival factor for chick ciliary neurons in vitro. More recently, it was shown to promote the survival of a variety of otherneuronal cell types and to affect the differentiation of E7 chick sympathetic neurons by inhibiting their proliferation and by inducing the expression of yasoactiYe intestinal peptide immunoreactiyity (VIP-IR). In cultures of dissociated sympathetic neurons from newborn rats, CNTF induces cholinergic differentiation as shown by increased levels of choline acetyltransferase (ChAT.},
  language  = {en}
}
@article{SolbachBogdanMolletal.1989,
  author    = {Solbach, W. and Bogdan, C. and Moll, Heidrun and Lohoff, M. and R{\"o}llinghoff, M.},
  title     = {Parasit{\"a}re Evasionsmechanismen: Beispiel Leishmanien},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30920},
  year      = {1989},
  abstract  = {Leishmanien besitzen eine Vielzahl von Mechanismen, die humorale und zellul{\"a}re Immunabwehr effektiv zu unterlaufen. Diese h{\"a}ngen eng mit der Expression von haupts{\"a}chlich zwei Glykokonjugaten auf der Parasitenoberfl{\"a}che zusammen, dem gp63 und dem Lipophosphoglykan. Die Parasiten sind einerseits schlechte Aktivatoren des alternativen Komplementweges und umgehen damit ihre eigene extrazellul{\"a}re Lyse. Oberfl{\"a}chengebundene Komplementfaktoren f{\"o}rdern andererseits die Aufnahme der Leishmanien durch Makrophagen. Solange diese nicht durch T-Zellen aktiviert sind, dienen sie den Parasiten als "Refugium". Dies gilt insbesondere, als Leishmanien in der Lage sind, 1. den "oxidative burst" zu hemmen; 2. toxische Sauerstoffmetaboliten zu entgiften; 3. abbauende lysosomale Enzyme zu hemmen und 4. das saure Milieu in den Lysosomen f{\"u}r ihren eigenen Metabolismus auszunutzen. Schließlich unterlaufen Leishmanien die zellul{\"a}re Immunabwehr des Wirts, indem sie die Aktivierung von T-Lymphozyten hemmen und die Expansion von T-Zell-Sub-populationen bewirken, die f{\"u}r ihr eigenes {\"U}berleben n{\"u}tzlich sind.},
  language  = {de}
}