@article{KrallmannWenzelOttHackeretal.1989, author = {Krallmann-Wenzel, U. and Ott, M. and Hacker, J{\"o}rg and Schmidt, G}, title = {Chromosomal mapping of genes encoding mannose-sensitive (type I) and mannose-resistant F8(P) fimbriae of Escherichia coli O18:K5:H5}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59545}, year = {1989}, abstract = {DNA hybridization experiments demonstrated that the gene clusters encoding the F8 fimbriae (fei) as well as the type I fimbriae (pi/) exist in a single copy on the chromosome of E. coli 018:K5 strain 2980. In conjugation experiments with appropriate donors, the chromosomal site of these gene clusters was determined. The pil genes were mapped close to the gene clusters thr and Jeu controlling the biosynthesis of threonine and leucine, respectively. The fei genes were found to be located close to the galactose operon (gal) between the position 17 and 21 of the E. coli chromosomallinkage map.}, subject = {Infektionsbiologie}, language = {en} } @article{HackerOttSchmidtetal.1986, author = {Hacker, J{\"o}rg and Ott, M. and Schmidt, G. and Hull, R. and Goebel, W.}, title = {Molecular cloning of the F8 fimbrial antigen from Escherichia coli}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59391}, year = {1986}, abstract = {The genetic determinant coding for the Pspecific F8 fimbriae was cloned from ยท the chromosome of the Escherichia coli wild-type strain 2980 (018: K5: H5: FlC, F8). The F8 determinant was further subcloned into the Pstl site of pBR322 and a restriction map was established. In a Southern hybridization experiment identity between the chromosomally encoded F8 determinant of 2980 and its cloned Counterpart was demonstrated. The cloned F8 fimbri{\"a}e and those of the wild type strain consist of a protein subunit of nearly 20 kDa. F8 fimbriated strains were agglutinated by an F8 polyclonal antiserum, caused mannose-resistant hemagglutination and attached to human uroepi thellal cells. The cloned F8 determinant was weil expressed in a variety of host strains.}, subject = {Infektionsbiologie}, language = {en} }