@phdthesis{Heinrichs2018,
  author    = {Heinrichs, Susanne Margarete},
  title     = {Myocardial B-cell infiltration following occlusion of the left anterior descending artery in mice is driven by CXCL13},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-168554},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2018},
  abstract  = {Myocardial B-cell infiltration after LAD occlusion in mice is driven by CXCL13 After myocardial infarction, the immune system is activated and regulates wound healing and remodeling processes in the heart. While the role of T cells has been elucidated already, the function of B cells in myocardial infarction remained relatively unclear until now. It is, however, already known that B cells are of importance in healing processes in other tissues, for example in the skin. Our studies therefore addressed the role and function of B cells in healing and early remodeling processes in the myocardium after infarction. Under physiological conditions, only few B cells can be found in the heart. After myocardial infarction, however, which we modelled with a permanent ligation of the left anterior descending artery (LAD) in C57BL/6J mice, we could demonstrate that B lymphocytes accumulate in the early phase after tissue injury (days one to seven) in the myocardium. To detect B cells, we performed immunofluorescence stainings on cryosections of infarcted hearts using an anti-B220 antibody. Quantitative analysis of tissue infiltration revealed that B cells peaked at day seven. In flow cytometry, we further characterized the B cells infiltrating infarcted tissue. We found that most of them were mature B cells (IgM+, IgD+). Next, we wanted to outline a potential mechanism responsible for B-cell infiltration to the site of tissue injury. We therefore performed ELISA experiments revealing that CXCL13 was upregulated in scar tissue. Antibody-mediated neutralization of CXCL13 verifiably attenuated B-cell infiltration. Treated mice also showed - in the tendency - smaller infarct sizes and an improved survival. In conclusion, we could show that B lymphocytes infiltrate the myocardium after MI in mice following a local CXCL13 gradient and that it is, most likely, beneficial to inhibit this process.},
  subject      = {Maus},
  language  = {en}
}
@article{LindemannRethwilm2011,
  author    = {Lindemann, Dirk and Rethwilm, Axel},
  title     = {Foamy Virus Biology and Its Application for Vector Development},
  series = {Viruses},
  volume    = {3},
  journal   = {Viruses},
  number    = {5},
  doi       = {10.3390/v3050561},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-139811},
  pages     = {561-585},
  year      = {2011},
  abstract  = {Spuma- or foamy viruses (FV), endemic in most non-human primates, cats, cattle and horses, comprise a special type of retrovirus that has developed a replication strategy combining features of both retroviruses and hepadnaviruses. Unique features of FVs include an apparent apathogenicity in natural hosts as well as zoonotically infected humans, a reverse transcription of the packaged viral RNA genome late during viral replication resulting in an infectious DNA genome in released FV particles and a special particle release strategy depending capsid and glycoprotein coexpression and specific interaction between both components. In addition, particular features with respect to the integration profile into the host genomic DNA discriminate FV from orthoretroviruses. It appears that some inherent properties of FV vectors set them favorably apart from orthoretroviral vectors and ask for additional basic research on the viruses as well as on the application in Gene Therapy. This review will summarize the current knowledge of FV biology and the development as a gene transfer system.},
  language  = {en}
}