@article{BoriskinDesyatskovaBogomolovaetal.1986,
  author    = {Boriskin, Yu S. and Desyatskova, R. G. and Bogomolova, N. N. and Gorboulev, Valentin G.},
  title     = {Stability of rubella virus after long-term persistence in human cell line},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46944},
  year      = {1986},
  abstract  = {Primary infection of HEp-2 cells with rubella virus resulted in non-cytophatic longterm persistent infection. During four years of persistence the virus was produced in sufficient quantities (up to 6 logs PFU/ml) and did not differ from the parental variant in its pathogenicity for BHK-21 or RK-13 cells, or hemagglutinating activity, but formed smaller plaques. Persistent virus preserved the original antigenicity as judged from reciprocal hemagglutination-inhibition or plaque reduction-neutralization tests with polyclonal antisera. Both original and persistent rubella viruses were thermoresistant (T 56° C) and sligthly temperature-sensitive. Clonal analysis revealed presence of ts-mutants among both original and persistent virus clones with different degrees of plating efficiency at 40°/34° C. RNA fingerprinting showed only minor changes in persistent rubella virus.},
  language  = {en}
}