@article{SubramanianDoeringKollertetal.2016,
  author    = {Subramanian, Hariharan and D{\"o}ring, Frank and Kollert, Sina and Rukoyatkina, Natalia and Sturm, Julia and Gambaryan, Stepan and Stellzig-Eisenhauer, Angelika and Meyer-Marcotty, Philipp and Eigenthaler, Martin and Wischmeyer, Erhard},
  title     = {PTH1R Mutants Found in Patients with Primary Failure of Tooth Eruption Disrupt G-Protein Signaling},
  series = {PLoS One},
  volume    = {11},
  journal   = {PLoS One},
  number    = {11},
  doi       = {10.1371/journal.pone.0167033},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-147967},
  pages     = {e0167033},
  year      = {2016},
  abstract  = {Aim Primary failure of tooth eruption (PFE) is causally linked to heterozygous mutations of the parathyroid hormone receptor (PTH1R) gene. The mutants described so far lead to exchange of amino acids or truncation of the protein that may result in structural changes of the expressed PTH1R. However, functional effects of these mutations have not been investigated yet. Materials and Methods In HEK293 cells, PTH1R wild type was co-transfected with selected PTH1R mutants identified in patients with PFE. The effects on activation of PTH-regulated intracellular signaling pathways were analyzed by ELISA and Western immunoblotting. Differential effects of wild type and mutated PTH1R on TRESK ion channel regulation were analyzed by electrophysiological recordings in Xenopus laevis oocytes. Results In HEK293 cells, activation of PTH1R wild type increases cAMP and in response activates cAMP-stimulated protein kinase as detected by phosphorylation of the vasodilator stimulated phosphoprotein (VASP). In contrast, the PTH1R mutants are functionally inactive and mutant PTH1R/Gly452Glu has a dominant negative effect on the signaling of PTH1R wild type. Confocal imaging revealed that wild type PTH1R is expressed on the cell surface, whereas PTH1R/Gly452Glu mutant is mostly retained inside the cell. Furthermore, in contrast to wild type PTH1R which substantially augmented K+ currents of TRESK channels, coupling of mutated PTH1R to TRESK channels was completely abolished. Conclusions PTH1R mutations affect intracellular PTH-regulated signaling in vitro. In patients with primary failure of tooth eruption defective signaling of PTH1R mutations is suggested to occur in dento-alveolar cells and thus may lead to impaired tooth movement.},
  language  = {en}
}
@article{SchlagenhaufJakobEigenthaleretal.2016,
  author    = {Schlagenhauf, Ulrich and Jakob, Lena and Eigenthaler, Martin and Segerer, Sabine and Jockel-Schneider, Yvonne and Rehn, Monika},
  title     = {Regular consumption of Lactobacillus reuteri-containing lozenges reduces pregnancy gingivitis: an RCT},
  series = {Journal of Clinical Periodontology},
  volume    = {43},
  journal   = {Journal of Clinical Periodontology},
  number    = {11},
  doi       = {10.1111/jcpe.12606},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-186783},
  pages     = {948-954},
  year      = {2016},
  abstract  = {Aim: This randomized controlled trial assessed the impact of Lactobacillus reuteri on pregnancy gingivitis in healthy women. Materials and Methods: Forty-five healthy women (24 test/21 placebo) with pregnancy gingivitis in the third trimester of pregnancy were enrolled. At baseline Gingival Index (GI) and Plaque Index (PlI) were assessed at the Ramfjord teeth and venous blood taken for TNF-alpha analysis. Subsequently participants were randomly provided with lozenges to be consumed 2 9 daily until birth (approx. 7 weeks) containing >= 10(8) CFU L. reuteri ATCC PTA 5289 and >= 10(8) CFU L. reuteri DSM 17938 (test) or being devoid of L. reuteri (placebo). Within 2 days after birth recording of GI, PlI and blood sampling were repeated. Results: At baseline, mean GI and mean PlI did not differ significantly between both groups. In the test group mean TNF-alpha serum level was significantly (p < 0.02) lower than in the placebo group. At reevaluation, mean GI and mean PlI of the test group were both significantly (p < 0.0001) lower than in the placebo group. Mean TNF-alpha serum level did no longer differ significantly between the groups. Conclusions: The consumption of L. reuteri lozenges may be a useful adjunct in the control of pregnancy gingivitis.},
  language  = {en}
}