@article{YousefStrzalkowskaHillenkampetal.2020,
  author    = {Yousef, Yousef Al and Strzalkowska, Alicja and Hillenkamp, Jost and Rosentreter, Andr{\´e} and Loewen, Nils A.},
  title     = {Comparison of a second-generation trabecular bypass (iStent inject) to ab interno trabeculectomy (Trabectome) by exact matching},
  series = {Graefe's Archive for Clinical and Experimental Ophthalmology},
  volume    = {258},
  journal   = {Graefe's Archive for Clinical and Experimental Ophthalmology},
  issn      = {0721-832X},
  doi       = {10.1007/s00417-020-04933-z},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-232613},
  pages     = {2775-2780},
  year      = {2020},
  abstract  = {Purpose To achieve a highly balanced comparison of trabecular bypass stenting (IS2, iStent inject) with ab interno trabeculectomy (T, Trabectome) by exact matching. Methods Fifty-three IS2 eyes were matched to 3446 T eyes. Patients were matched using exact matching by baseline intraocular pressure (IOP), the number of glaucoma medications, and glaucoma type, and using nearest neighbor matching by age. Individuals without a close match were excluded. All surgeries were combined with phacoemulsification. Results A total of 78 eyes (39 in each group) could be matched as exact pairs with a baseline IOP of 18.3 ± 5.1 mmHg and glaucoma medications of 2.7 ± 1.2 in each. IOP in IS2 was reduced to 14.6 ± 4.2 mmHg at 3 months and in T to a minimum of 13.1 ± 3.2 mmHg at 1 month. In IS2, IOP began to rise again at 6 months, eventually exceeding baseline. At 24 months, IOP in IS2 was 18.8 ± 9.0 mmHg and in T 14.2 ± 3.5 mmHg. IS2 had a higher average IOP than T at all postoperative visits (p < 0.05 at 1, 12, 18 months). Glaucoma medications decreased to 2.0 ± 1.5 in IS2 and to 1.5 ± 1.4 in T. Conclusion T resulted in a larger and sustained IOP reduction compared with IS2 where a rebound occurred after 6 months to slightly above preoperative values.},
  language  = {en}
}
@article{WaxmanStrzalkowskaWangetal.2023,
  author    = {Waxman, Susannah and Strzalkowska, Alicja and Wang, Chao and Loewen, Ralitsa and Dang, Yalong and Loewen, Nils A.},
  title     = {Tissue-engineered anterior segment eye cultures demonstrate hallmarks of conventional organ culture},
  series = {Graefe's Archive for Clinical and Experimental Ophthalmology},
  volume    = {261},
  journal   = {Graefe's Archive for Clinical and Experimental Ophthalmology},
  number    = {5},
  doi       = {10.1007/s00417-022-05915-z},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-323845},
  pages     = {1359-1368},
  year      = {2023},
  abstract  = {Background Glaucoma is a blinding disease largely caused by dysregulation of outflow through the trabecular meshwork (TM), resulting in elevated intraocular pressure (IOP). We hypothesized that transplanting TM cells into a decellularized, tissue-engineered anterior segment eye culture could restore the outflow structure and function. Methods Porcine eyes were decellularized with freeze-thaw cycles and perfusion of surfactant. We seeded control scaffolds with CrFK cells transduced with lentiviral vectors to stably express eGFP and compared them to scaffolds seeded with primary TM cells as well as to normal, unaltered eyes. We tracked the repopulation behavior, performed IOP maintenance challenges, and analyzed the histology. Results Transplanted cells localized to the TM and progressively infiltrated the extracellular matrix, reaching a distribution comparable to normal, unaltered eyes. After a perfusion rate challenge to mimic a glaucomatous pressure elevation, transplanted and normal eyes reestablished a normal intraocular pressure (transplanted = 16.5 ± 0.9 mmHg, normal = 16.9 ± 0.9). However, eyes reseeded with eGFP-expressing CrFK cells could not regulate IOP, remaining high and unstable (27.0 ± 6.2 mmHg) instead. Conclusion Tissue-engineered anterior segment scaffolds can serve as readily available, scalable ocular perfusion cultures. This could reduce dependency on scarce donor globes in outflow research and may allow engineering perfusion cultures with specific geno- and phenotypes.},
  language  = {en}
}