@article{LinsenmairLinsenmair1971, author = {Linsenmair, Karl Eduard and Linsenmair, Christa}, title = {Paarbildung und Paarzusammenhalt bei der monogamen W{\"u}stenassel Hemilepistus reaumuri (Crustacea, Isopoda, Oniscoidea)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33937}, year = {1971}, abstract = {No abstract available}, language = {de} } @article{GrafeLinsenmair1989, author = {Grafe, U. and Linsenmair, Karl Eduard}, title = {Protogynous sex change in the Reed Frog: Hyperolius viridiflavus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30990}, year = {1989}, abstract = {Observations on captive reed frogs Hyperolius viridijlavus ommatostictus showed that seven out of 24 females changed into males. Sex change occurred without any hormone treatment and resulted in completely functional males. The adaptive value is discussed in terms of maximizing life-time reproductive success. Hyperolius r. ommatostictus is the first amphibian known to show functional sex reversal.}, language = {en} } @article{SchmuckGeiseLinsenmair1994, author = {Schmuck, R. and Geise, W. and Linsenmair, Karl Eduard}, title = {Life cycle strategies and physiological adjustments of Reedfrog Tadpoles (Amphibia, Anura, Hyperoliidae) in relation to environmental conditions.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31009}, year = {1994}, abstract = {The relationship between different degrees of intraspecific crowding of reedfrog tadpoles and their physiological responses to a deterioration of the natal pond water quality was examined under laboratory conditions. Tadpoles that were reared at a lower density metamorphosed significantly earlier than those raised at a higher density. As density increases, the average body length at metamorphosis decreases. However, at low tadpole density, a significantly higher diversity of body size classes among freshly metamorphosed froglets was observed than under more crowded conditions. Mortality increased during metamorphic climax and was inversely correlated with the tadpole density. In ephemeral ponds, an accumulation of nitrogenous wastes from metabolic processes and/or a concentration by evaporation in prolonged rainless periods can pose a considerable chemical stress to reedfrog tadpoles. Hyperolius viridiflavus ommatostictus responded to an increasing ammonia concentration with an activity increase of the ornithine cycle (intensified urea synthesis). hi contrast, Hyperolius marmoratus taeniatus exhibited a strong tolerance against high ammonia levels. A deterioration of the natal pond water quality caused H. v. ommatostictus and H. v. nitidulus tadpoles to adjust to harsher climatic conditions at the time of metamorphosis. This physiological preadjustment enabled the froglets to start feeding and growing immediately after metamorphosis even at low air humidity and rare precipitation events. In contrast, froglets that were raised in daily refreshed water exhibited high mortality rates if subjected to identical conditions. As one possible indicator of the actual climatic conditions prevailing in the surrounding terrestrial habitat, fluctuations in the water ammonia level are discussed.}, language = {en} } @article{TackeSchuberthBeckeretal.1982, author = {Tacke, M. and Schuberth, W. and Becker, Charles R. and Haas, L. D.}, title = {The dielectric constant of PbTe at 4.2 K and \(\tilde ν\)=84.15 cm\(^{-1}\), 96.97 cm\(^{-1}\), 103.60 cm\(^{-1}\)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30821}, year = {1982}, abstract = {The dielectric constant of a PbTe epitaxial layer has been measured by surface wave spectroscopy using an optically pumped far-infrared laser and the technique of attenuated total reflection.}, language = {en} } @article{GeisGeickBeckeretal.1977, author = {Geis, G. and Geick, R. and Becker, Charles R. and Wagner, V.}, title = {Antiferromagnetic resonance in CoO/NiO mixed crystals}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31239}, year = {1977}, abstract = {We have studied the lowest magnetic excitation of Ni\(_{1-x}\)Co\(_x\)O mixed crystals for 0.94 \(\leq\) x \(\leq\) 1. Together with previous results for 0.02 \(\leq\) x \(\leq\) 0.07 and neutron data for x = 0.14 and x = 0.30, the results are discussed by means of a model, especially the variation of AFMR frequency and preferred spin direction with Co concentration x.}, language = {en} } @article{Becker1970, author = {Becker, Charles R.}, title = {Impurity-induced absorption in or near the phonon gap of KI}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30757}, year = {1970}, abstract = {In or near the phonon gap of KI a single weak line due to OW (OD\(^-\)) at 69.7 (69.3) cm\(^{-1}\) has been observed. A second and much stronger line at 94.1 cm\(^{-1}\) was shown not to be related to OH\(^-\), but instead is thought to be due to CO\(_3\).}, language = {en} } @article{KoehlerBecker1974, author = {K{\"o}hler, H. and Becker, Charles R.}, title = {Optically active lattice vibrations in Bi\(_2\)Se\(_3\)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31296}, year = {1974}, abstract = {The preparation of Bi2Sea single crystals with low free carrier densities allowed an investigation of the lattice vibrations to be carried out from the reflectivity of cleavage planes at nearly normal incidence of the radiation (E ~ c). The experimental results ean be explained with two classical oscillators, whose eigenfrequencies oceur at 92 and 69.5 cm-I. The static dielectric constant was determined to be 100 ± 10 for E ~ c.}, language = {en} } @article{KlitzingBecker1976, author = {Klitzing, K. von and Becker, Charles R.}, title = {Far infrared photoconductivity of residual acceptors in pure tellurium}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31278}, year = {1976}, abstract = {The photoconductivity of both undeformed and deformed Te samples has been investigated at liquid He temperature by means of a Fourier spectrometer. Three peaks were usually found in the spectra of undeformed samples at 11, 24 and 46 cm\(^{-1}\). These are shown to be due to three different chemical impurities. The deformed samples are characterized by additional structure at higher frequencies.}, language = {en} } @article{Becker1971, author = {Becker, Charles R.}, title = {Evidence for a quasi-phonon gap in CsCl}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30767}, year = {1971}, abstract = {The observed impurity induced far-infrared absorption in CsCl : Rb\(^+\) and CsCl : K\(^+\) is compared with a calculated density of acoustic phonon states in CsCl. The absorption due to CsCl : Rb\(^+\) displays a minimum between the acoustic and optic phonon bands. A narrow line is observed in CsCl: K\(^+\) at 85.8 cm\(^{-1}\) which falls in this quasi-phonon gap.}, language = {en} } @article{ReimerScheerPetersetal.1986, author = {Reimer, Georg and Scheer, Ulrich and Peters, Jan-Michael and Tan, Eng M.}, title = {Immunolocalization and partial characterization of a nucleolar autoantigen (PM-Scl) associated with polymyositis / scleroderma overlap syndromes.}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33191}, year = {1986}, abstract = {Precipitating anti-PM-Sel antibodies are present in sera from patients with polymyositis. scleroderma. and polymyositis/scleroderma overlap syndromes. By indirect immunofluorescence microscopy. anti-PM-Scl antibodies stained the nucleolus in cells of different tissues and species. suggesting that the antigen is highly conserved. By electron microscopy, anti-PM-Scl antibodies reacted primarily with the granular component of the nuc1eolus. Drugs that inhibit rRNA synthesis had a marked effect on the expression of PM-Scl antigen. In actinomycin D-treated cells, immunofluorescence staining by anti-PM-Scl was sign{\"u}icantly reduced with residual staining restricted to the granular regions of nuc1eoli. Treatment with 5,6-dichloro-beta-D- ribofuranosylbenzimidazole (DRB) also selectively reduced nuc1eolar staining. On a molecular level, anti-PM-Sel antibodies precipitated 11 polypeptides with molecular weights (Mr) ranging from 110,000 to 20,000. The Mr 80,000 and 20.000 polypeptides were phosphorylated. Evidence suggests that the PM-Scl antigen complex may be related to a prerlbosomal particle.}, language = {en} } @article{ScheerSommervilleBustin1979, author = {Scheer, Ulrich and Sommerville, John and Bustin, M.}, title = {Injected histone antibodies interfere with transcription of lampbrush chromosome loops in oocytes of Pleurodeles}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33166}, year = {1979}, abstract = {Antibodies to calf thymus histone H2B were purified by chromatography on DEAE-cellulose and injected into oocyte nuclei of Pleurodeles waltlii. As shown by indirect immunofluorescence these antibodies cross-reacted strongly with corresponding histones associated with lampbrush chromosomes. Shortly after injection the lateral loops of the chromosomes retracted into the chromomeres and by 3 h postinjection the 'lampbrush' appearance was completely lost and the chromosomes appeared in light-microscopic preparations as rod-like structures consisting of 10ngitudina11y coalesced chromomeres. In control oocytes injected with non-immune immunoglobulins or antibodies against a ubiquitous transcript-associated protein no morphological alterations of the lampbrush chromosomes could be observed. Electron microscopic spreads of chromosomes prepared at various times after injection of anti-H2B revealed a progressive loss of transcriptional complexes from the loop axes. Finally, higher-order chromatin configurations, like supranuc1eosomal globules (' superbeads ') or cable-like chromatin strands 50- 60 nm thick predominated, indicating complete transcriptional inactivation of a11 chromosomal regions. The results indicate that H2B antibodies react specifically with his tones associated with the transcribed DNA of lateral loops in their native state. The resulting antigenantibody complexes seem to inhibit progression of the R A polymerases along the template, thus causing the premature release of transcripts, a process analogous to the stripping effect of actinomycin D. The demonstration of histones associated with heavily transcribed regions, which are not compacted into nucleosomes but largely extended, supports the current concept that unfolding of nucleosomes to a110w transcription of the DNA does not involve dissociation of histones. In contrast, amplified ribosomal RNA genes are unaffected by injected HzB antibodies. This does not necessarily indicate absence of his tones from nucleolar chromatin, since we do not know whether it is accessible in vivo to antibodies or whether the histone antigenie determinants are masked by the presence of other proteins. The technique of injecting specific antibodies should be widely applicable when analysing the in vivo distribution of chromosomal components at the electron-microscopic level and when studying complex metabolie processes, like the cleavage and modification of RNA, by selective inhibition of defined enzymic steps.}, language = {en} } @article{ReimerRoseScheeretal.1987, author = {Reimer, Georg and Rose, Kathleen M. and Scheer, Ulrich and Tan, Eng M.}, title = {Autoantibody to RNA polymerase I in scleroderma sera}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34294}, year = {1987}, abstract = {Autoantibodies to components of the nucleolus are a unique serological feature of patients with scleroderma. There are autoantibodies of several specificities; one type produces a speckled pattern of nucleolar staining in immunofluorescence. In actinomycin D and 5,6-dichloro-{j-D-ribofuranosylbenzimidazoletreated Vero cells, staining was restricted to the fibrillar and not the granular regions. By double immunofluorescence, specific rabbit anti-RNA polymerase I antibodies stained the same fibrillar structures in drug-segregated nucleoli as scleroderma sera. Scleroderma sera immunoprecipitated 13 polypeptides from (35S)methionine-labeled HeLa cell extract with molecular weights ranging from 210,000 to 14,000. Similar polypeptides were precipitated by rabbit anti-RNA polymerase I antibodies, and their common identities were confirmed in immunoabsorption experiments. Microinjection of purified IgG from a patient with speckled nucleolar staining effectively inhibited ribosomal RNA transcription. Autoantibodies to RNA polymerase I were restricted to certain patients with scleroderma and were not found in other autoimmune diseases.}, language = {en} } @article{ReinhardHalbrueggeScheeretal.1992, author = {Reinhard, Matthias and Halbr{\"u}gge, Maria and Scheer, Ulrich and Wiegand, Christiane and Jockusch, Brigitte M. and Walter, Ulrich}, title = {The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34246}, year = {1992}, abstract = {Vasoactive agents which elevate either cGMP or cAMP inhibit platelet activation by pathways sharing at least one component, the 46/50 kDa vasodilator-stimulated phosphoprotein (V ASP). V ASP is stoichiometrically phosphorylated by both cGMP-dependent and cAMPdependent protein kinases in intact human platelets, and its phosphorylation correlates very well with platelet inhibition caused by cGMP- and cAMP-elevating agents. Here we report that in human platelets spread on glass, V ASP is associated predominantly with the distal parts of radial micro filament bundles and with microfilaments outlining the periphery, whereas less V ASP is associated with a central microfilamentous ring. V ASP is also detectable in a variety of different cell types including fibroblasts and epithelial cells. In fibroblasts, V ASP is concentrated at focal contact areas, along microfilament bundles (stress fibres) in a punctate pattern, in the periphery of protruding lamellae, and is phosphorylated by cGMP- and cAMP-dependent protein kinases in response to appropriate stimuli. Evidence for the direct binding of V ASP to F -actin is also presented. The data demonstrate that V ASP is a novel phosphoprotein associated with actin filaments and focal contact areas, i.e. transmembrane junctions between microfilaments and the extracellular matrix.}, language = {de} } @article{ScheerDabauvalleMerkertetal.1988, author = {Scheer, Ulrich and Dabauvalle, Marie-Christine and Merkert, Hilde and Benavente, Ricardo}, title = {The nuclear envelope and the organization of the pore complexes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34275}, year = {1988}, abstract = {No abstract available}, language = {en} } @article{SchubertKrausKenkliesetal.1992, author = {Schubert, D.W. and Kraus, M.M and Kenklies, R. and Becker, Charles R. and Bicknell-Tassius, R.N.}, title = {Composition and wavelength dependence of the refractive index in Cd\(_{1-x}\)Mn\(_x\)Te epitaxial layers}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-37716}, year = {1992}, abstract = {We have investigated Cd\(_{1-x}\)Mn\(_x\)Te thin films with Mn concentrations of x=0.12, 0.18, 0.30, 0.52, and 0.70. These single crystal layers were grown by molecular beam epitaxy on [001] CdTe substrates. The real part of the refractive index, n, was determined below the band-gap Eo in the range of 0.5-2.5 eV at T=300 K. The parallel reOectivity was measured near the Brewster angle at the YAG laser wavelength of 1.064 J.Lm (hv= 1.165 eV). Combining these results with the optical pathlength results (nd) of reOection measurements in a Fourier spectrometer we have determined n(x,v) over a wide spectral range by utilizing a three parameter fit. The accuracy of these results for n should improve waveguide designs based on this material.}, language = {en} } @article{BellDabauvalleScheer1992, author = {Bell, Peter and Dabauvalle, Marie-Christine and Scheer, Ulrich}, title = {In vitro assembly of prenucleolar bodies in Xenopus egg extract}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34233}, year = {1992}, abstract = {Nuclei assembled in Xenopus egg extract from purified DNA or chromatin resemble their natural counterparts in a number of structural and functional features. However, the most obvious structural element of normal interphase nuclei, the nucleolus, is absent from the in vitro reconstituted nuclei. By EM, cytological silver staining, and immunofluorescence microscopy employing antibodies directed against various nucleolar components we show that nuclei assembled in vitro contain numerous distinct aggregates that resemble prenucleolar bodies (PNBs) by several criteria. Formation of these PNB-like structures requires pore complex-mediated nuclear transport of proteins but is independent of the genetic content of the in vitro nuclei as well as transcriptional and translational events. Our data indicate that nuclei assembled in vitro are capable of initiating early steps of nucleologenesis but that the resulting PNBs are unable to fuse with each other, probably due to the absence of a functional nucleolus organizer. With appropriate modifications, this experimental system should be useful to define and analyze conditions promoting the site-specific assembly of PNBs into a coherent nucleolar body.}, language = {en} } @article{Scheer1981, author = {Scheer, Ulrich}, title = {Identification of a novel class of tandemly repeated genes transcribed on lampbrush chromosomes of Pleurodeles waltlii}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33153}, year = {1981}, abstract = {Electron microscope preparations of lampbrush chromosomes from oocytes of Pleurodeles waltl;; have revealed a new class of tandemly repeated genes. These genes are highly active, as judged by the close spacing of nascent transcripts. They occur in clusters of >100 copies and are transcribed in units containing roughly 940 base pairs of DNA that are separated by nontranscribed spacers of an estimated DNA content of 2,410 base pairs. The size and the pattern of arrangement of these transcription units can not be correlated with any of the repetitious genes so far described.}, language = {en} } @article{FrankeKleinschmidtSpringetal.1981, author = {Franke, Werner W. and Kleinschmidt, J{\"u}rgen A. and Spring, Herbert and Krohne, Georg and Grund, Christine and Trendelenburg, Michael F. and St{\"o}hr, Michael and Scheer, Ulrich}, title = {A nucleolar skeleton of protein filaments demonstrated in amplified nucleoli of Xenopus laevis}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33130}, year = {1981}, abstract = {The amplified, extrachromosomal nucleoli of Xenopus oocytes contain a meshwork of -4-nm-thick filaments, which are densely coiled into higher-order fibrils of diameter 30-40 nm and are resistant to treatment with high- and low-salt concentrations, nucleases (DNase I, pancreatic RNase, micrococcal nuclease), sulfhydryl agents, and various nonionic detergents. This filamentous "skeleton" has been prepared from manually isolated nuclear contents and nucleoli as weil as from nucleoli isolated by fluorescence-activated particle sorting. The nucleolar skeletons are observed in light and electron microscopy and are characterized by ravels of filaments that are especially densely packed in the nucleolar cortex. DNA as weil as RNA are not constituents of this structure, and precursors to ribosomal RNAs are completely removed from the extraction-resistant filaments by treatment with high-salt buffer or RN ase. Fractions of isolated nucleolar skeletons show specific enrichment of an acidic major protein of 145,000 mol wt and an apparent pi value of -6.15, accompanied in some preparations by various amounts of minor proteins. The demonstration of this skeletal structure in "free" extrachromosomal nucleoli excludes the problem of contaminations by nonnucleolar material such as perinucleolar heterochromatin normally encountered in studies of nucleoli from somatic cells. It is suggested that this insoluble protein filament complex forms a skeleton specific to the nucleolus proper that is different from other extraction-resistant components of the nucleus such as matrix and lamina and is involved in the spatial organization of the nucleolar chromatin and its transcriptional products. In studies of the organization of the interphase nucleus, considerable progress has been made in the elucidation of the arrangement of chromatin components and transcriptional products. However, relatively little is known about the composition and function of another category of nuclear structures, the nonnucleoproteinaceous architectural components that are insoluble in solutions of low and high ionic strength, despite numerous studies dedicated to this problem. Such structures include (a) the nuclear envelope and its pore complexes (I, 15, 18, 23, 37, 41), (b) a peripheral layer of insoluble protein ("lamina"; I, 15, 22, 23, 59), (e) certain skeletal proteins related to the chromosome "scaffold" described by Laemmli and coworkers (see references 2 and 3), and (d) ill-defined tangles of fibrillar structures of the nuclear interior that are collectively described as residual "matrix" (6, 21 ; for reviews, see references THE JOURNAL OF CEll BrOlOGY . VOlUME 90 AUGUST 1981 289-299 © The RockefeIler University Press · 0021 -9525/ 81 / 08/ 0289/ 11 \$1 .00 4 and 12). The latter, preparatively}, language = {en} } @article{FrankeScheerKrohneetal.1981, author = {Franke, Werner W. and Scheer, Ulrich and Krohne, Georg and Jarasch, Ernst-Dieter}, title = {The nuclear envelope and the architecture of the nuclear periphery}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33108}, year = {1981}, abstract = {No abstract available}, language = {en} } @article{BonaScheerBautz1981, author = {Bona, Marion and Scheer, Ulrich and Bautz, Ekkehard K. F.}, title = {Antibodies to RNA polymerase II (B) inhibit transcription in lampbrush chromosomes after microinjection into living amphibian oocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-33128}, year = {1981}, abstract = {Antibodies directed against RNA polymerase II (B) from Drosophila melanogaster were obtained from rabbit sera and, as monoclonal immunoglobulins, from mouse hybridomas and shown to cross-react with the amphibian enzyme protein. Localization by indirect immunofluorescence microscopy revealed the association of this enzyme with chromatin of interphase nuclei of amphibian cells and its absence in nucleoli. Purified immunoglobulins were microinjected in to nuclei ofliving vitellogenic oocytes of Ple1lrodeles waltlii and X enopus laevis and their effects on transcriptional processes were monitored by biochemical and light and electron microscopic stud ies. RNA polymerase II antibodies from rabbit sera caused a rapid and almost complete release of nascent transcripts from the chromatin axis of the loops of lampbrush chromosomes, followed by collapse of the loops and their retraction on the main chromosome axis. Monoclonal murine antibodies to the Iarge RNA polymerase II subunits also inhibited transcription in chromosome Ioops but appeared to inhibit initiation rather than elongation events. Activities of class land III RNA polymerases were not significantly affected by injection of antibodies to polymerase II, indicating immunological differences between the three RNA polymerases. The potential value of the in vitro test system described , as a very sensitive assay for detecting proteins involved in transcription in living cells, is discussed. 1}, language = {en} }