@article{DindasDreyerHuangetal.2021,
  author    = {Dindas, Julian and Dreyer, Ingo and Huang, Shouguang and Hedrich, Rainer and Roelfsema, M. Rob G.},
  title     = {A voltage-dependent Ca\(^{2+}\) homeostat operates in the plant vacuolar membrane},
  series = {New Phytologist},
  volume    = {230},
  journal   = {New Phytologist},
  number    = {4},
  doi       = {10.1111/nph.17272},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-259627},
  pages     = {1449-1460},
  year      = {2021},
  abstract  = {Cytosolic calcium signals are evoked by a large variety of biotic and abiotic stimuli and play an important role in cellular and long distance signalling in plants. While the function of the plasma membrane in cytosolic Ca\(^{2+}\) signalling has been intensively studied, the role of the vacuolar membrane remains elusive. A newly developed vacuolar voltage clamp technique was used in combination with live-cell imaging, to study the role of the vacuolar membrane in Ca\(^{2+}\) and pH homeostasis of bulging root hair cells of Arabidopsis. Depolarisation of the vacuolar membrane caused a rapid increase in the Ca\(^{2+}\) concentration and alkalised the cytosol, while hyperpolarisation led to the opposite responses. The relationship between the vacuolar membrane potential, the cytosolic pH and Ca2+ concentration suggests that a vacuolar H\(^{+}\)/Ca\(^{2+}\) exchange mechanism plays a central role in cytosolic Ca2+ homeostasis. Mathematical modelling further suggests that the voltage-dependent vacuolar Ca\(^{2+}\) homeostat could contribute to calcium signalling when coupled to a recently discovered K\(^{+}\) channel-dependent module for electrical excitability of the vacuolar membrane.},
  language  = {en}
}