@phdthesis{Trippen2022,
  author    = {Trippen, Raimund Dieter},
  title     = {Ph{\"a}notypisierung von Effektor T-Zellen bei Juveniler Idiopathischer Arthritis (JIA)},
  doi       = {10.25972/OPUS-25202},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-252026},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2022},
  abstract  = {Background: Juvenile Idiopathic Arthritis (JIA) is a heterogeneous disease with unknown etiology of arthritis for more than six weeks in patients aged under 16 years. Human Cytomegalovirus (HCMV) is a lymphotropic betaherpesvirus that persists in the human body and causes ongoing stimulation of the effector T-cell system. For both, JIA and HCMV, a premature immunosenescence is shown. Aim: To investigate the potential influence of HCMV on the prematurely altered immune system of JIA patients. Methods: T-cell phenotype, intracellular cytokine production and the expression of chemokine receptors were measured by flow cytometry (FACS). HCMV serostatus was measured by enzyme-linked immunosorbent assay (ELISA). Phenotype and cytokine production of lymphocytes derived from JIA patients and healthy donors were compared regarding their HCMV serostatus. Results: Both JIA patients and healthy donors showed an association between HCMV seropositivity and immunosenescence resulting in low proportions of naive T-cells and relatively higher proportions of differentiated T-cells. Within the JIA patients HCMV seropositivity was associated with higher intracellular IFNγ production. T-cells in JIA patients showed a higher CCR5 expression in association with HCMV seropositivity. This association was not seen in healthy donors. Conclusion: The T-cell phenotype was similarly associated with HCMV in JIA patients and healthy donors. In contrast, JIA patients showed evidence of TH1 predominance in association with HCMV seropositivity. Regarding CCR5 this effect is significantly stronger in JIA patients than in healthy donors. The present study suggests that HCMV associated changes of the T-cell differentiation may be corroborated in JIA patients.},
  subject      = {Juvenile idiopathische Arthritis},
  language  = {de}
}
@phdthesis{Sustal2020,
  author    = {Sustal, Klara Nela},
  title     = {T-Zell Hom{\"o}ostase und Plastizit{\"a}t in Juveniler idiopathischer Arthritis},
  doi       = {10.25972/OPUS-12386},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-123863},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2020},
  abstract  = {Juvenile idiopathic arthritis (JIA) is a chronic systemic inflammatory disease in childhood with unknown etiology. Previous studies have demonstrated an important role for CD4+ T helper cells. The aim of the present study was to investigate the T cell phenotype and cytokine profile in children with JIA with focus on disease activity. T cells from peripheral blood mononuclear cells of 84 children with JIA and 40 age-matched healthy donors (HD) were analyzed by flowcytometry. T cells from JIA patients were less differentiated with higher numbers of na{\"i}ve T cells compared to HD. Cytokine profile analysis revealed a reduced intracellular Th1- and Th2-specific cytokine production. Significantly higher levels of RORуt expression, the most important transcription factor of Th17 cells, were found during acute disease flare. Moreover, IL-17 production was significantly higher in patients with active disease compared to patients in remission. Furthermore, stimulation experiments with CCR6+CD4+ T cells, which we could identify as IL-17 producing cells, revealed distinct plasticity. Polarization of isolated CCR6+ cells into Th17, Th1 or regulatory T cells, respectively, was achieved using specific cytokines. Interestingly, the potential to polarize differed in JIA patients and healthy donors. T cells from JIA patients showed a more stable Th17 phenotype and restricted switching to Th1 or Treg. These results suggest a predominant Th17 phenotype of T cells in JIA patients. During disease flares, RORуt seems to be an important factor leading to Th17 polarization. An opposing modulation of Th1, Th2 or Treg was not found. In conclusion, a Th17 phenotype dominates during disease flares in JIA and underlines the role of inflammatory T cells in the etiopathogenesis of JIA. IL-17, CCR6 and RORуt, Th17-specific factors, may be interesting targets for novel therapeutic approaches in JIA.},
  subject      = {Juvenile chronische Arthritis},
  language  = {de}
}
@phdthesis{Bieber2020,
  author    = {Bieber, Marlene Barbara},
  title     = {Impfstatus von Patienten mit Juveniler idiopathischer Arthritis},
  doi       = {10.25972/OPUS-20558},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-205581},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2020},
  abstract  = {Patienten mit JIA erhielten weniger Lebendimpfungen als gesunde Kontrollpersonen, f{\"u}r Totimpfstoffe waren die Immunisierungsraten vergleichbar. Ebenso zeigten die Antik{\"o}rper Konzentrationen zwischen den JIA Patienten und den gesunden Kontrollpersonen keine signifikanten Unterschiede. Bei Untersuchungen innerhalb der JIA Patienten Gruppe zeigte lediglich der Abstand zwischen der letzten Auffrischungsimpfung und der Blutentnahme signifikante Ergebnisse.},
  subject      = {Juvenile chronische Arthritis},
  language  = {de}
}
@phdthesis{Fischer2011,
  author    = {Fischer, Michael Johannes},
  title     = {K{\"o}rperliche Leistungsf{\"a}higkeit bei Patienten mit HLA B27 positiver juveniler idiopathischer Arthritis in Remission},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-67301},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2011},
  abstract  = {Mit dieser Arbeit sollte untersucht werden, ob es eine Beeintr{\"a}chtigung der k{\"o}rperlichen Leistungsf{\"a}higkeit bei Patienten bis zum 20. Lebensjahr mit inaktiver juveniler idiopathischer Arthritis bzw. einer Arthritis in Remission im Vergleich zu gesunden Gleichaltrigen gibt und wenn ja, ob ein Zusammenhang zu dem Eiweißk{\"o}rper HLA B27 besteht.},
  subject      = {Juvenile chronische Arthritis},
  language  = {de}
}
@phdthesis{SuessFroehlich2004,
  author    = {S{\"u}ß-Fr{\"o}hlich, Yvonne},
  title     = {Die Serumpr{\"a}valenz von Parvovirus B19 bei rheumatischen Erkrankungen im Kindesalter},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-13418},
  school      = {Universit{\"a}t W{\"u}rzburg},
  year      = {2004},
  abstract  = {Ziel dieser Studie war es, einen m{\"o}glichen Zusammenhang zwischen einer Parvovirus B19-Infektion und der {\"A}tiologie und Pathogenese der Juvenilen idiopathischen Arthritis (JIA) zu untersuchen. Hierzu wurden von insgesamt 382 Patienten der Rheumasprechstunde der Kinderpoliklinik der Universit{\"a}t W{\"u}rzburg Serumproben auf das Vorhandensein von Parvovirus B19-IgG-Antik{\"o}rpern gestestet. Der Nachweis dieser Antik{\"o}rper erfolgte mittels indirektem Immunfluoreszenztest (IFT). Das gleiche Verfahren wurde auch auf 146 gesunde Kontrollpatienten angewandt. Das Patientenkollektiv wurde diagnostisch in 13 Subgruppen unterteilt, die Kontrollen in drei altersentsprechende Gruppen. Die Gruppe Systemischer Lupus Erythematodes (SLE) n=4 zeigte die h{\"o}chste Parvovirus B19-Serumpr{\"a}valenz mit 100\%. Die Gruppe Enthesitis assoziierte Arthritis (EAA) n=54 wies mit 72,2\% eine hohe Pr{\"a}valenz auf. Im mittleren Bereich lagen die Gruppen Lyme Arthritis n=37 (67,6\%), Arthralgien n=85 (62,4\%), Polyarthritis n=19 (57,9\%), andere, nicht n{\"a}her klassifizierbare Arthritis n=28 (57,1\%), CRMO n=12 (50\%), Psoriasis Arthritis n=11 (63,6\%), Morbus Still n=8 (62,5\%) und andere Oligoarthritis -nicht EOPA-JIA n=13 mit 53,9\% positiv getesteten Patienten. Die niedrigsten Serumpr{\"a}valenzen erzielten die Subgruppen reaktive Arthritis n=38 (39,5\%), fr{\"u}hkindliche Oligoarthritis (EOPA-JIA) n=67 (31,3\%) und rheumatische Augenerkrankung n=6 (33,3\%). Im Vergleich zu den Kontrollgruppen konnte in keiner der Subgruppen ein statistisch signifikant h{\"o}heres Ergebnis ermittelt werden. Lediglich in den Gruppen reaktive Arthritis und EOPA-JIA wurden statistisch signifikante Unterschiede erreicht, die jedoch niedriger als in den entsprechenden Kontrollgruppen lagen. Aus diesem Grund untermauert das Resultat dieser Studie nicht die Hypothese eines pathogenetischen Zusammenhangs zwischen einer Parvovirus B19-Infektion und kindlichem Rheuma.},
  language  = {de}
}