@article{MunzJakobBorisjuk2016, author = {Munz, Eberhard and Jakob, Peter M. and Borisjuk, Ljudmilla}, title = {The potential of nuclear magnetic resonance to track lipids in planta}, series = {Biochimie}, volume = {130}, journal = {Biochimie}, doi = {10.1016/j.biochi.2016.07.014}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-186828}, pages = {97-108}, year = {2016}, abstract = {Nuclear Magnetic Resonance (NMR) provides a highly flexible platform for non invasive analysis and imaging biological samples, since the manipulation of nuclear spin allows the tailoring of experiments to maximize the informativeness of the data. MRI is capable of visualizing a holistic picture of the lipid storage in living plant/seed. This review has sought to explain how the technology can be used to acquire functional and physiological data from plant samples, and how to exploit it to characterize lipid deposition in vivo. At the same time, we have referred to the current limitations of NMR technology as applied to plants, and in particular of the difficulty of transferring methodologies optimized for animal/medical subjects to plant ones. A forward look into likely developments in the field is included, anticipating its key future role in the study of living plant.}, language = {en} } @article{BorisjukRolletschekFuchsetal.2011, author = {Borisjuk, Ljudmilla and Rolletschek, Hardy and Fuchs, Johannes and Melkus, Gerd and Neuberger, Thomas}, title = {Low and High Field Magnetic Resonance for \(in\) \(Vivo\) Analysis of Seeds}, series = {Materials}, volume = {4}, journal = {Materials}, number = {8}, doi = {10.3390/ma4081426}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-140910}, pages = {1426-1439}, year = {2011}, abstract = {Low field NMR has been successfully used for the evaluation of seed composition and quality, but largely only in crop species. We show here that 1.5T NMR provides a reliable means for analysing the seed lipid fraction present in a wide range of species, where both the seed size and lipid concentration differed by >10 fold. Little use of high field NMR has been made in seed research to date, even though it potentially offers many opportunities for studying seed development, metabolism and storage. Here we demonstrate how 17.5T and 20T NMR can be applied to image seed structure, and analyse lipid and metabolite distribution. We suggest that further technical developments in NMR/MRI will facilitate significant advances in our understanding of seed biology.}, language = {en} }