@article{LehrnbecherMerzSebaldetal.1991,
author = {Lehrnbecher, T. and Merz, H. and Sebald, Walter and Poot, M.},
title = {Interleukin 4 drives phytohemagglutinin-activated T cells through several cell cycles: no synergism between interleukin 2 and interleukin 4},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62491},
year = {1991},
abstract = {Cell kinetic studies of T cells stimulated with the interleukin 2 (11-2), D-4, or both lymphokines were performed with conventional [3H] thymidine incorporation and with the bivariate BrdU/Hoechst technique. 11-2 and 11-4 are able to drive phytohemagglutininactivated T cells through more than one cell cycle. Neither synergistic nor inhibitory efl'ect on T -cell proliferationwas seen for the stimulation with both 11-2 and 11-4 as compared with the effect ofll-2 alone. The quantitative data ofthe cell cycle distribution ofphytohemagglutininactivated T cells suggestthat the population ofll-4-responsive cells is at least an overlapping population, if not a real subset of the ·population of the 11-2-responsive cells.},
subject = {Biochemie},
language = {en}
}
@article{MerzFliednerOrschescheketal.1991,
author = {Merz, H. and Fliedner, A. and Orscheschek, K. and Binder, T. and Sebald, Walter and M{\"u}ller-Hermelink, H. K. and Feller, A. C.},
title = {Cytokine expression in T-cell lymphomas and Hodgkin's disease. Its possible implication in autocrine or paracrine production as a potential basis for neoplastic growth},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62483},
year = {1991},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{KruseLehrnbecherSebald1991,
author = {Kruse, N. and Lehrnbecher, T. and Sebald, Walter},
title = {Site-directed mutagenesis reveals the importance of disulfide bridges and aromatic residues for structure and proliferative activity of human interleukin-4},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62505},
year = {1991},
abstract = {Mutant proteins (muteins) of human lnterleukin-4 (llA) were constructed by means of in vitro mutagenesis. The muteins were expressed in E. co/1, submitted to a renaturation and purification protocol and analysed for biological activity. Exchange of the cysteines at either position 46 or 99 which form one of the three disulfide bridges resulted. in a nearly co•mplete loss · of biological actiyity and an unstable protein. The exchange of tyrosine 124 also inactivated the protein, while a mutation of tyrosine 56 left some residual activity. Exchange of the other four cysteines or of · the single tryptophane had smaller etTects.},
subject = {Biochemie},
language = {en}
}
@article{PreiserSchmartzVanderLindenetal.1991,
author = {Preiser, J. C. and Schmartz, D. and Van der Linden, P. and Content, J. and Vanden Bussche, P. and Buurman, W. and Sebald, Werner and Dupont, E. and Pinsky, M. R. and Vincent, J. L.},
title = {Interleukin-6 administration has no acute hemodynamic or hematologic effect in the dog},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62511},
year = {1991},
abstract = {To investigate the possible hemodynamic efl'ects of interleukin-6 (IL-6), a single dose of 15 mcg/kg of recombinant IL-6 isolated from Escherichia coli was injected intravenously in six pentobarbital-anesthetized dogs. After 30 min, saline infusion was performed to maintain the - pulmonary artery balloon-occluded pressure at baseline Ievel. The animals were observed for up to 5 hours. No other hemodynamic alteration was observed than a gradual decline in cardiac output attributed to anesthesia. Hematologic variables, blood glucose, and total serum proteins were also constant. IL-6 levels were markedly elevated in the blood, bot no tumor necrosis factor activity was detected. Thus a primary role for IL-6 in the early cardiovascular alterations associated with septic shock seems unlikely.},
subject = {Biochemie},
language = {en}
}
@article{TonyLehrnbecherMerzetal.1991,
author = {Tony, H. P. and Lehrnbecher, T. and Merz, H. and Sebald, Werner and Wilhelm, M.},
title = {Regulation of IL-4 responsiveness in lymphoma B cells},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62520},
year = {1991},
abstract = {The responsiveness to IL-4 with and without costimulation with anti-IgM antibodies or phorbolester was studied in 35 cases of low grade non-Hodgkin Iymphoma by analyzing enhancement of CD23 and HLA dass li expression. The predominant phenotype responds directly to IL-4. Separate differentiation states can be distinguished according to coordinate or differential upregulation of CD23 and HLA dass II molecules by IL-4 alone, and differences in responsiveness to anti-IgM antibodies. A particular subgroup of B-lymphoma cells defines a separate stage of B-eeil differentiation. They fail to express high affinity binding sites for IL-4 and accordingly do not respond to IL-4- mediated signals. Cross-linking membrane lgM receptors or direct activation of protein kinase C via phorbolester induces IL-4 receptor expression and subsequent IL-4 reactivity.},
subject = {Biochemie},
language = {en}
}
@article{FoernzlerWittbrodtSchartl1991,
author = {F{\"o}rnzler, Dorothee and Wittbrodt, Joachim and Schartl, M.anfred},
title = {Analysis of an esterase linked to a locus involved in the regulation of the melanoma oncogene and isolation of polymorphic marker sequences in Xiphophorus},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61726},
year = {1991},
abstract = {Melanoma formation in Xiphophorus hybrids is mediated by a growth factor receptor tyrosine kinase oncogene encoded by the Tu locus. In the wild-type parental fish no tumors occur due to the activity of a locus that regulates the activity of the melanoma oncogene. Molecu/ar identification of this regulatory locus (R) requires a precise physical map of the chromosomal region. Therefore we studied esterase isozymes in Xiphophorus, two of which have been previously reported to be linked to locus R. We confinn that ES 1 is a distant marker for R ( approx. 30cM), and contrary to earlier studies, we show that this isozyme is present in all species of the genus and at similar activity Ievels in all organs tested. ES4, which has also been reported to be linked to R, was found to be a misclassification of liver ES1. In an attempt to identify markersthat bridge the large distance between ESl and R, we have generated DNA probes which are highly polymorphic. They will be useful in finding Iandmarks on a physical map of the R-containing chromosomal region.},
subject = {Physiologische Chemie},
language = {en}
}
@article{SchartlSchluppSchartletal.1991,
author = {Schartl, Manfred and Schlupp, Ingo and Schartl, Angelika and Meyer, Manfred K. and Nanda, Indrajit and Schmid, Michael and Epplen, J{\"o}rg T. and Parzefall, Jakob},
title = {On the stability of dispensable constituents of the eukaryotic genome: Stability of coding sequences versus truly hypervariable sequences in a clonal vertebrate, the amazon molly, Poecilia formosa},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61731},
year = {1991},
abstract = {In dooal unisexual vertebrales, the genes specifying the males become dispensable. To study tbe rate of such geoes the gynogeoetic all-female fisb Poecilillfonnolll was treated with androgens. Phenotypic males were obtained that exbibited the complete set of male cbaracteristics of dosely related gooocboristic species, induding body proportions, pigmentation, the extremely complex insemination apparatus of poecil{\"u}d fish, sexual bebavior, and spermatogeoesls. Tbe apparent stabllity of such genic structures, induding those involved in androgen regulation, is contrasted by high instability of noncoding sequeaces. Frequent mutations, thelr donal transmission, and at least two truly hypervariable Iod leading to individual difl'ereaces between these othenrise donal organisms were detected by DNA fingerprinting. These observations substantiate the concept that also in "ameiotic" vertebrates certain compartments of the genome are more prooe to mutatiooal alterations than others.},
subject = {Physiologische Chemie},
language = {en}
}
@article{WinklerVielkindSchartl1991,
author = {Winkler, Christoph and Vielkind, J{\"u}rgen R. and Schartl, Manfred},
title = {Transient expression of foreign DNA during embryonic and larval development of the medaka fish (Oryzias latipes)},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61743},
year = {1991},
abstract = {Species of small fish are becoming useful tools for studies on vertebrate development. Wehave investigated the developing embryo of the Japanese medaka for its application as a transient expression system for the in vivo analysis of gene regulation and function. The temporaland spatial expression patterns ofbacterial chloramphenicol acetyltransferase and galactosidase reporter genes injected in supercoiled plasmid form into the cytoplasm of one cell of the two-cell stage embryo was promoter-specific. The transient expression was found to be mosaic within the tissue and organs reflecting the unequal distribution of extrachromosomal foreign DNA and the intensive cell mixing movements that occur in fish embryogenesis. The expression data are consistent with data on DNA fate. Foreign DNA persisted during embryogenesis and was still detectable in some 3- and 9-month-old adult fish; it was found in high molecular weight form as weil as in circular plasmid conformations. The DNA was replicated during early and late embryogenesis. Our data indicate that the developing medaka embryo is a powerful in vivo assay system for studies of gene regulation and function.},
subject = {Physiologische Chemie},
language = {en}
}
@article{HaasBrehmKreftetal.1991,
author = {Haas, Albert and Brehm, Klaus and Kreft, J{\"u}rgen and Goebel, Werner},
title = {Cloning, characterization, and expression in Escherichia coli of a gene encoding Listeria seeligeri catalase, a bacterial enzyme highly homologous to mammalian catalases},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60536},
year = {1991},
abstract = {A gene coding for catalase (hydrogen-peroxide:hydrogen-peroxide oxidoreductase; EC 1.11.1.6) of the grain-positive bacterium Listeria seeligeri was cloned from a plasmid library of EcoRI-digested chromosomal DNA, with Escherichia coli DHSa as a host. The recombinant catalase was expressed in E. coli to an enzymatic activity approximately SO times that of the combined E. coli catalases. The nucleutide sequence was determined, and the deduced amino acid sequence revealed 43.2\% amino acid sequence identity between bovine liver catalase and L. seeligeri catalase. Most of the amino acid residues which are involved in catalytic activity, the formation of the active center accession channel, and heme binding in bovine liver catalase were also present in L. seeligeri catalase at the corresponding positions. The recombinant protein contained 488 amino acid residues and had a calculated molecular weight of 55,869. The predicted isoelectric point was 5.0. Enzymatic and genetic analyses showed that there is most probably a single catalase of this type in L. seeligeri. A perfect 21-bp inverted repeat, which was highly homologous to previously reported binding sequences of the Fur (ferric uptake regulon) protein of E. coli, was detected next to the putative promoter region of tbe L. seeligeri catalase gene.},
subject = {Biologie},
language = {en}
}
@article{SchueleinKreftGonskietal.1991,
author = {Sch{\"u}lein, Ralf and Kreft, J{\"u}rgen and Gonski, Sigrid and Goebel, Werner},
title = {Preprosubtilisin Carlsberg processing and secretion is blocked after deletion of amino acids 97-101 in the mature part of the enzyme},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60577},
year = {1991},
abstract = {During an investigation into the substrate specificity and processing of subtilisin Carlsberg from Bacillus licheniformis, two major independent findings were made: (i) as has been shown previously, a stretch of five amino acids (residues 97-101 of the mature enzyme) that loops out into the binding cleft is involved in substrate binding by subtilisin Carlsberg. In order to see whether this loop element also determines substrate specificity, the coding region for these five amino acids was deleted from the cloned gene for subtilisin Carlsberg by site-directed mutagenesis. Unexpectedly the resulting mutant preproenzyme (P42c, Mr=42 kDa) was not processed to the mature form (Mr = 30 kDa) and was not released into the medium by a proteasedeficient B. subtilis host strain; rather, it accumulated in the cell membrane. This result demonstrates that the integrity of this loop element, which is very distant from the processing cleavage sites in the preproenzyme, is required for secretion of subtilisin Carlsberg. (ii) In culture supernatants from B. subtilis harbouring the cloned wild-type subtilisin Carlsberg gene the transient appearance (at 0-3 h after onset of stationary phase) of a processing intermediate (P38c, Mr = 38 kDa) oftbis protease could be demonstrated. P38c very probably represents a genuine proform of subtilisin Carlsberg.},
subject = {Biologie},
language = {en}
}
@article{VortkampThiasGessleretal.1991,
author = {Vortkamp, A. and Thias, U. and Gessler, Manfred and Rosenkranz, W. and Kroisel, P. M. and Tommerup, N. and Kruger, G. and Gotz, J. and Pelz, L. and Grzeschik, Karl-Heinz},
title = {A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59217},
year = {1991},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{DabauvalleLoosMerkertetal.1991,
author = {Dabauvalle, Marie-Christine and Loos, Karin and Merkert, Hilde and Scheer, Ulrich},
title = {Spontaneous assembly of pore complex-containing membranes ("Annulate lamellae") in Xenopus egg extract in the absence of chromatin},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32797},
year = {1991},
abstract = {No abstract available},
language = {en}
}
@article{VortkampGesslerGrzeschik1991,
author = {Vortkamp, Andrea and Gessler, Manfred and Grzeschik, Karl-Heinz},
title = {GLI3 zinc-finger gene interrupted by translocations in Greig syndrome families},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30100},
year = {1991},
abstract = {No abstract available},
language = {de}
}
@article{DandekarTollervey1991,
author = {Dandekar, Thomas and Tollervey, D.},
title = {Thirty-three nucleotides of 5' flanking sequence including the TATA box are necessary and sufficient for efficient U2 snRNA transcription in Schizosaccharomycespombe},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29959},
year = {1991},
abstract = {No abstract available},
language = {en}
}
@article{DandekarStripeckeGrayetal.1991,
author = {Dandekar, Thomas and Stripecke, Renata and Gray, Nicola K. and Goossen, Britta and Constable, Anne and Johansson, Hans E. and Hentze, Matthias W.},
title = {Identification of a novel iron-responsive element in murine and human erythroid \(\delta\)-aminolevulinic acid synthase mRNA},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29929},
year = {1991},
abstract = {No abstract available},
language = {en}
}
@article{MaschwitzFialaMoogetal.1991,
author = {Maschwitz, Ulrich and Fiala, Brigitte and Moog, J. and Saw, L. G.},
title = {Two new myrmecophytic associations from the Malay Peninsula: ants of the genus Cladomyrma as partners of Saraca thaipingensis and Crypteronia griffithii},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32992},
year = {1991},
abstract = {In Peninsular Malaysia the trees Saraca thaipingensis (Caesalpiniaceae) and Crypteronia griffithii (Crypteroniaceae) are inhabited by ants. In the vicinity ofGombak, near Kuala Lumpur, the hollow internodes of young Saraca thaipingensis plants are colonized mainly by two Cladomyrma species. In larger trees a Crematogaster sp. is also found. Crypteronia griffithii is inhabited by a third species of Cladomyrma. None of these species is conspecific with any of the three Cladomyrma taxa so far described. The colonies are founded by single mated queens, which have a conspicuous, sphecid wasp-like behaviour when searching for host plants and nest sites. They chew holes into the plant intern odes and hollow them out to provide nest sites. Coccids and pseudococcids are cultivated within the internodes. The homopterans are not carried by queens on their nuptial flights. They apparently find their way by themselves into the cavities or are perhaps carried there by the worker ants. The Cladomyrma ants on Crypteronia are not aggressive, in contrast to those on Saraca thaipingensis. The relationship of Crypteronia with ants seems to be obligatory, whereas Saraca was only partly colonized by Cladomyrma. The interaction of Saraca with Crematogaster sp. is loose and facultative, since the Crematogaster sp. also lives on other tree species. Our studies have now revealed four Cladomyrma spp. which are regularly associated with plants. The genus therefore seems to have an entirely myrmecophytic way of life.},
language = {en}
}
@article{Linsenmair1991,
author = {Linsenmair, Karl Eduard},
title = {Allokation elterlicher Investition beim Bienenwolf Philantus triangulum (Hymenoptera: Sphecidae)},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-78191},
year = {1991},
abstract = {No abstract available},
subject = {Zoologie},
language = {de}
}
@article{SchluppParzefallSchartl1991,
author = {Schlupp, I. and Parzefall, J. and Schartl, Manfred},
title = {Male mate choice in mixed bisexual/-unisexual breeding complexes of Poecilia (Teleostei: Poeciliidae)},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-80309},
year = {1991},
abstract = {The livebearing all-female fish Poecilia formosa reproduces by gynogenesis, a modified form of parthenogenesis. P. formosa forms at least two breeding complexes: in its northern range it exists sympatrically with Poecilia latipinna and in its southern range with Poecilia mexicana. Differences between these complexes and their possible origin are discussed. Embryogenesis is triggered by sperm of males of these closely related sympatric species. Because inheritance is stricdy maternal, from the male point of view energy and time invested are totally lost. In this study we wanted to elucidate whether males are able to distinguish between conspecific and parasitic females. It could be shown that males are able to distinguish females optically, but that this ability was obscured as soon as chemical and/or tactile contact was possible. Furthermore, we found that females in an attractive phase of their sexual cycle are always preferred, regardless of species. This is possibly the mechanism by which parasitic females obtain the matings they need to reproduce.},
subject = {Poecilia (Teleostei: Poeciliidae)},
language = {en}
}
@article{MalitschekSchartl1991,
author = {Malitschek, B. and Schartl, Manfred},
title = {Rapid identification of recombinant baculoviruses using PCR},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-80328},
year = {1991},
abstract = {no abstract available},
subject = {PCR},
language = {en}
}
@article{Fiala1991,
author = {Fiala, Brigitte},
title = {Partnerschaften von Pflanzen und Ameisen: Ameisenb{\"a}ume im malaysischen Regenwald.},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42936},
year = {1991},
abstract = {No abstract available},
language = {de}
}