@article{VortkampThiasGessleretal.1991,
author = {Vortkamp, A. and Thias, U. and Gessler, Manfred and Rosenkranz, W. and Kroisel, P. M. and Tommerup, N. and Kruger, G. and Gotz, J. and Pelz, L. and Grzeschik, Karl-Heinz},
title = {A somatic cell hybrid panel and DNA probes for physical mapping of human chromosome 7p},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59217},
year = {1991},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{MerzFliednerLehrnbecheretal.1990,
author = {Merz, H. and Fliedner, A. and Lehrnbecher, T. and Sebald, Walter and M{\"u}ller-Hermelink, H. K. and Feller, A. C.},
title = {Cytokine expression in B-cell non-Hodgkin lymphomas},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62539},
year = {1990},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{GesslerHameisterHenryetal.1990,
author = {Gessler, Manfred and Hameister, H. and Henry, I. and Junien, C. and Braun, T. and Arnold, H. H.},
title = {The human MyoD1 (MYF3) gene maps on the short arm of chromosome 11 but is not associated with the WAGR locus or the region for the Beckwith-Wiedemann syndrome},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59221},
year = {1990},
abstract = {The human gene encoding the myogenic determination factor myf3 (mouse MyoD1) has been mapped to the short arm of chromosome 11. Analysis of several somatic cell hybrids containing various derivatives with deletions or translocations revealed that the human MyoD (MYF3) gene is not associated with the WAGR locus at chromosomal band 11pl3 nor with the loss of the heterozygosity region at 11p15.5 related to the Beckwith-Wiedemann syndrome. Subregional mapping by in situ hybridization with an myf3 specific probe shows that the gene resides at the chromosomal band llp14, possibly at llp14.3.},
subject = {Biochemie},
language = {en}
}
@article{vanHeyningenBickmoreSeawrightetal.1990,
author = {van Heyningen, V. and Bickmore, W. A. and Seawright, A. and Fletcher, J. M. and Maule, J. and Fekete, G. and Gessler, Manfred and Bruns, G. A. and Huerre-Jeanpierre, C. and Junien, C.},
title = {Role for the Wilms tumor gene in genital development?},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59238},
year = {1990},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{WeigelMeyerSebald1989,
author = {Weigel, U. and Meyer, M. and Sebald, Walter},
title = {Mutant proteins of human interleukin 2. Renaturation yield, proliferative activity and receptor binding},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62543},
year = {1989},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{FlueggeFischerGrossetal.1989,
author = {Fl{\"u}gge, U. I. and Fischer, K. and Gross, A. and Sebald, Walter and Lottspeich, F. and Eckerskorn, C.},
title = {The triose phosphate-3-phosphoglycerate-phosphate translocator from spinach chloroplasts: nucleotide sequence of a full-length cDNA clone and import of the in vitro synthesized precursor protein into chloroplasts},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62559},
year = {1989},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{GesslerBruns1989,
author = {Gessler, Manfred and Bruns, G. A. P.},
title = {A physical map around the WAGR complex on the short arm of chromosome 11},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59246},
year = {1989},
abstract = {A long-range restriction map of part of the short arm of ehromosome 11 including the WAGR region has been constructed using pulsed-field gel electrophoresis and a number of infrequently cutting restriction enzymes. A total of 15.4 Mbp has been mapped in detall, extending from proximal 11p14 to the distal part of 11p12. The map localizes 35 different DNA probes and reveals at least nine areas with features eharaeteristle of BTF islands, some of which may be candidates for the different loci underlying the phenotype of the WAGR syndrome. This map will furthermore allow screening of DNA from individuals with WAGR-related phenotypes and from Wilms tumors for associated chromosomal rearrangements.},
subject = {Biochemie},
language = {en}
}
@article{GesslerThomasCouillinetal.1989,
author = {Gessler, Manfred and Thomas, G. H. and Couillin, P. and Junien, C. and McGillivray, B. C. and Hayden, M. and Jaschek, G. and Bruns, G. A.},
title = {A deletion map of the WAGR region on chromosome II},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59255},
year = {1989},
abstract = {The WAGR (Wilms tumor, aniridia, genitourinary anomalies, and mental retardation) region has been assigned to chromosome 11p13 on the basis of overlapping constitutional deletions found in affected individuals. We have utilized 31 DNA probes which map to the WAGR deletion region, together with six reference loci and 13 WAGR-related deletions, to subdivide this area into 16 intervals. Specific intervals have been correlated with phenotypic features, leading to the identification of individual subregions for the aniridia and Wilms tumor loci. Delineation, by specific probes, of multiple intervals above and below the critical region and of five intervals within the overlap area provides a framework map for molecular characterization of WAGR gene loci and of deletion boundary regions.},
subject = {Biochemie},
language = {en}
}
@article{GesslerBruns1988,
author = {Gessler, Manfred and Bruns, Gail A. P.},
title = {Molecular mapping and cloning of the breakpoints of a chromosome 11p14.1-p13 deletion associated with the AGR syndrome},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59264},
year = {1988},
abstract = {Chromosome 11p13 is frequently rearranged in individuals with the WAGR syndrome (Wilms tumor, aniridia, genitourinary anomalies, and mental retardation) or parts of this syndrome. To map the cytogenetic aberrations molecularly, we screened DNA from cell Unes with known WAGR-related chromosome abnormalities for rearrangements with pulsed fleld gel (PFG) analysis using probes deleted from one chromosome 11 homolog of a WAGR patient. The first alteration was detected in a cell line from an individual with aniridia, genitourinary anomalies, mental retardation, and a deletion described as 11p14.1-p13. We have located one breakpoint close to probe HU11-164B and we have cloned both breakpoint sites as well as the junctional fragment. The breakpoints subdivide current intervals on the genetic map, and the probes for both sides will serve as important additional markers for a long-range restriction map of this region. Further characterization and sequencing of the breakpoints may yield insight into the mechanisms by which these deletions occur.},
subject = {Biochemie},
language = {en}
}
@article{KleenePfannerPfalleretal.1987,
author = {Kleene, R. and Pfanner, N. and Pfaller, R. and Link, T. A. and Sebald, Walter and Neupert, W. and Tropschug, M.},
title = {Mitochondrial porin of Neurospora crassa: cDNA cloning, in vitro expression and import into mitochondria},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62566},
year = {1987},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{RoemischTropschugSebaldetal.1987,
author = {R{\"o}misch, J. and Tropschug, M. and Sebald, Walter and Weiss, H.},
title = {The primary structure of cytochrome c\(_1\) from Neurospora crassa},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62578},
year = {1987},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{BarnekowGessler1986,
author = {Barnekow, Angelika and Gessler, Manfred},
title = {Activation of the pp60\(^{c-src}\) kinase during differentiation of monomyelocytic cells in vitro},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59278},
year = {1986},
abstract = {Tbe proto-oncogene c-src, the cellular homolog of the Rous sarcoma virus (RSV) transforming gene v-src, is expressed in a tissue-specific and age-dependent manner. Its physiological function, although still unknown, appears to be more closely related to differentiation processes than to proliferation processes. To obtain more information about the physiological role of the c-src gene in cells, we have studied differentiation-dependent alterations using the human HL-60 leukaemia cell line as a model system. Induction of monocytic and granulocytic differentiation of HL-60 cells by 12-0-tetradecanoylphorbol-13-acetate (TPA) and dimethylsulfoxide (DMSO) is associated with an activation of the pp60c-src tyrosine kinase, but not with increased c-src gene expression. Control experiments exclude an interaction of TPA and DMSO themselves with the pp60c-src kinase.},
subject = {Biochemie},
language = {en}
}
@article{WeichSebaldSchaireretal.1986,
author = {Weich, H. A. and Sebald, Walter and Schairer, H. U. and Hoppe, J.},
title = {The human osteosarcoma cell line U-2 OS expresses a 3.8 kilobase mRNA which codes for the sequence of the PDGF-B chain},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62588},
year = {1986},
abstract = {A cDNA clone of about 2500 basepairswas prepared from the human osteosarcoma cellline U-2 OS by hybridizing with a v-sis probe. Sequence analysis showed that this cDNA contains the coding region for the PDGF-B chain. Here we report that the mitogen secreted by these osteosarcoma cells contains the PDGF-B chain and is probably a homodimer of two B-chains.},
subject = {Biochemie},
language = {en}
}
@article{HoppeGattiWeberetal.1986,
author = {Hoppe, J. and Gatti, D. and Weber, H. and Sebald, Walter},
title = {Labeling of individual amino acid residues in the membrane-embedded F\(_0\) part of the F\(_1\) F\(_0\) ATP synthase from Neurospora crassa. Influence of oligomycin and dicyclohexylcarbodiimide},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62598},
year = {1986},
abstract = {Three F0 subunits and the F\(_1\) subunit P of the ATP synthase from Neurospora crassa were labeled with the lipophilic photoactivatable reagent 3-(trifluoromethyl)-3-(m-[\(^{125}\)I]iodophenyl)diazirine ([\(^{125}\)I]TID). In the proteolipid subunit which was the most heavily labeled polypeptide labeling was confmed to five residues at the NH2-terminus and five residues at the C-terminus ofthe protein. Labeling occurred at similar positions compared with the homologaus protein (subunit c) in the ATP synthase from Escherichia coli, indicating a similar structure of the proteolipid subunits in their respective organisms. The inhibitors oligomycin and dicyclohexylcarbodiimide did not change the pattern of accessible surface residues in the proteolipid, suggesting that neither inhibitor induces gross conformational changes. However, in the presence of oligomycin, the extent oflabeling in some residues was reduced. Apparently, these residues provide part of the binding site for the inhibitor. After reaction with dicyclohexylcarbodiimide an additional labeled amino acid was found at position 65 corresponding to the invariant carbod{\"u}mide-binding glutamic acid. These results and previous observations indicate that the carboxyl side chain of Glu-65 is located at the protein-lipid interphase. The idea is discussed that proton translocation occurs at the interphase between different types if F\(_0\) subunits. Dicyclohexylcarbodiimide or oligomycin might disturb this essential interaction between the F\(_0\) subunits.},
subject = {Biochemie},
language = {en}
}
@article{HoppeSebald1986,
author = {Hoppe, J. and Sebald, Walter},
title = {Topological studies suggest that the pathway of the protons through F\(_0\) is provided by amino acid residues accessible from the lipid phase},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62602},
year = {1986},
abstract = {The structure of the F0 part of ATP synthases from E. coli and Neurospora crassa was analyzed by hydrophobic surface labeling with [125I]TID. In the E. co/i F0 all three subunits were freely accessible to the reagent, suggesting that these subunits are independently integrated in the membrane. Labeted amino acid residues were identified by Edman degradation of the dicyclohexylcarbodiimide binding (DCCD) proteins from E. coli and Neurospora crassa. The very similar patterns obtained with the two homologaus proteins suggested the existence of tightly packed cx-helices. The oligomeric structure of the DCCD binding protein appeared to be very rigid since little, if any, change in the labeling patternwas observed upon addition of oligomycin or DCCD to membranes from Neurospora crassa. When membrancs were pretrcated with DCCD prior to the reaction with [125I]TID an additionally labeled amino acid appeared at the position of Glu·65 which binds DCCD covalently, indicating the Jocation of this inhibitor on the outside of the oligomer. It is suggested that proton conduction occurs at the surface of the oligomer of the DCCD binding protein. Possibly this oligomer rotates against the subunit a or b and thus enables proton translocation. Conserved residues in subunit a, probably located in the Iipid bilayer, might participate in the pro· ton translocation mechanism.},
subject = {Biochemie},
language = {en}
}
@article{GabelliniSebald1986,
author = {Gabellini, N. and Sebald, Walter},
title = {Nucleotide sequence and transcription of the fbc operon from Rhodopseudomonas sphaeroides. Evaluation of the deduced amino acid sequences of the FeS protein, cytochrome b and cytochrome c\(_1\)},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62615},
year = {1986},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{McCarthySebaldGrossetal.1986,
author = {McCarthy, J. E. and Sebald, Walter and Gross, G. and Lammers, R.},
title = {Enhancement of translational efficiency by the Escherichia coli atpE translational initiation region: its fusion with two human genes},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62626},
year = {1986},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{HarnischWeissSebald1985,
author = {Harnisch, U. and Weiss, H. and Sebald, Walter},
title = {The primary structure of the iron-sulfur subunit of ubiquinol-cytochrome c reductase from Neurospora, determined by cDNA and gene sequencing},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62631},
year = {1985},
abstract = {No abstract available},
subject = {Biochemie},
language = {en}
}
@article{GabelliniHarnischMcCarthyetal.1985,
author = {Gabellini, N. and Harnisch, U. and McCarthy, J. E. and Hauska, G. and Sebald, Walter},
title = {Cloning and expression of the fbc operon encoding the FeS protein, cytochrome b and cytochrome c\(_1\) from the Rhodopseudomonas sphaeroides b/c\(_1\) complex},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62642},
year = {1985},
abstract = {The gene for the FeS protein of the Rhodopseudomonas sphaeroides b/c1 complex was identified by means of crosshybridization with a segment of the gene encoding the corresponding FeS protein of Neurospora crassa. Plasmids (pRSF1-14) containing the cross-hybridizing region, covering in total 13.5 kb of chromosomal DNA, were expressed in vitro in a homologous system. One RSF plasmid directed the synthesis of all three main polypeptides of the R. sphaeroides blc1 complex: the FeS protein, cytochrome b and cytochrome c1• The FeS protein and cytochrome c1 were apparently synthesized as precursor fonns. None of the pRSF plasmids directed the synthesis of the 10-kd polypeptide found in b/c1 complex preparations. Partial sequencing of the cloned region was performed. Several sites of strong homology between R. sphaeroides and eukaryotic polypeptides of the b/c1 complex were identified. The genes encode the three b/c1 polypeptides in the order: (5') FeS protein, cytochrome b, cytochrome c1• The three genes are transcribed to give a polycistronic mRNA of 2.9 kb. This transcriptional unit has been designated the jbc operon; its coding capacity corresponds to the size of the polycistronic mRNA assuming that only the genes for the FeS protein (jbcF), cytochrome b (jbcß) and cytochrome c1 (jbcC) are present. This could indicate that these three subunits constitute the minimal catalytic unit of the b/c1 complex from photosynthetic membranes.},
subject = {Biochemie},
language = {en}
}
@article{McCarthySchairerSebald1985,
author = {McCarthy, J. E. and Schairer, H. U. and Sebald, Walter},
title = {Translational initiation frequency of atp genes from Escherichia coli: identification of an intercistronic sequence that enhances translation},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62657},
year = {1985},
abstract = {The c, b and {\"o} subunit genes of the Escherichia coli atp operon were cloned individually in an expression vector between the tac fusion promoter and the galK gene. The relative rates of subunit synthesis directed by the cloned genes were similar in vitro andin vivo and compared favourably with the subunit stoichiometry of the assembled proton-translocating A TP synthase of E. coli in vivo. The rate of synthesis of subunit c was at least six times that of subunit b and 18 times that of subunit {\"o}. Progressive shortening of the long intercistronic sequence lying upstream of the subunit c gene showed that maximal expression of this gene is dependent upon the presence of a sequence stretching > 20 bp upstream of the Shine-Dalgarno site. This sequence thus acts to enhance the rate of translational initiation. The possibility that similar sequences might perform the same function in other operons of E. coli and bacteriophage A is also discussed. Translation of the subunit b cistron is partially coupled to translation of the preceding subunit c cistron. In conclusion, the expression of all the atp operon genes could be adjusted to accommodate the subunit requirements of A TP synthase assembly primarily by means of mechanisms which control the efficiency of translational initiation and re-initiation at the respective cistron start codons.},
subject = {Biochemie},
language = {en}
}