@article{SyldatkStoffregenWuttkeetal.1988,
  author    = {Syldatk, C. and Stoffregen, A. and Wuttke, F. and Tacke, Reinhold},
  title     = {Enantioselective reduction of acetyldimethylphenylsilane: a screening with thirty strains of microorganisms},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63906},
  year      = {1988},
  abstract  = {Thirty strains of microorganisms (bacteria, yeasts, fungi and green algae) were tested as resting free cells for their ability to transform acetyldimethylphenylsilane (1) enantioselectively into (R)-(1-hydroxyethyl) dimethylphenylsilane [(R)-2]. The biotransformations were monitared by GC (packed OV-17 column), and the enantiomeric purities of the products isolated were determined by HPLC (cellulose triacetate column, UV detection). All microorganisms tested were found to reduce 1 enantioselectively to give (R)-2. Under the test conditions used, the yeast Trigonapsis variabilis (DSM 70714) was found to 1 exhibif the highest specific activity (1.5 mg product x g cell wet mass\(^{-1}\) x min\(^{-1}\) ), whereas the highest enantioselectivities were observed for the bacteria Acinetobacter ca lcoaceticus (ATCC 31012) (>95\% ee), Brevfbacterium species (ATCC 21860) (90\% ee) and Corynebacterium dioxydans (ATCC 21766) (>95\% ee), the yeast Candida humico la (OSM 70067) (90\% ee), the fungus Cunninghame lla e legans (ATCC 26269) (94\% ee), as well as the cyanobacterium Synechococcus leopoliensis (94\% ee).· From the green algae tested, Chlamydomonas reinhardii showed the highest.enantioselectivity (85\% ee).},
  subject      = {Anorganische Chemie},
  language  = {en}
}
@article{SyldatkAndreeStoffregenetal.1987,
  author    = {Syldatk, C. and Andree, H. and Stoffregen, A. and Wagner, F. and Stumpf, B. and Ernst, L. and Zilch, H. and Tacke, Reinhold},
  title     = {Enantioselective reduction of acetyldimethylphenylsilane by Trigonopsis variabilis (DSM 70714)},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-63836},
  year      = {1987},
  abstract  = {Growing and resting cells of the yeast Trigonapsis variabilis (DSM 70714) can be used for the enantioselective reduction of the organosilicon compound acetyldimethylphenylsilane (J) to give optically active (R)-(1-hydroxyethyl)dimethylphenylsilane [(R)-2] in good yields. The enantiomeric purity of the isolated product was determined tobe 62-86\% ee depending on the substrate concentration used. Both substrate and product caused an inhibition of the reaction at concentrations higher than 0.35 and 0.5 g/1, respectively. Besides, higher substrate and product concentrations led to increased formation of the by-product 1,1,3,3-tetramethyl-1,3-diphenyldisiloxane. Considering the limiting substrate and product concentrations, it was possible to use the same biomass at least 5 times without significant loss of enzyme activity. 3-Methyl-3-phenyl-2-butanone (5) and acetyldimethylphenylgermane (7), which represent carbon and germanium analogues of 1, were also found to be accepted as substrates by Trigonapsis variabilis (DSM 70714). The reduction rates of the silicon {1) and germanium compound {7) were much higher than the transformation rate of the corresponding carbon analogue 5.},
  subject      = {Anorganische Chemie},
  language  = {en}
}