@inproceedings{AxelrodGorboulevKutateladzeetal.1976,
author = {Axelrod, V. D. and Gorboulev, Valentin G. and Kutateladze, T. V. and Barciszewski, J. and Bayev, A. A.},
title = {The new approach to tRNA primary structure determination : the primary structure of valine tRNA\(^{Val}_{2b}\)},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-50920},
year = {1976},
abstract = {The new combination of TLC and high voltage electrophoresis on cooling plate is described.We have applied this technique to study of primary structure of tRNA.Preliminary sequence of baker's yeast tRNA^Val_2b is described. New approach to preparation of large tRNA fragments is demonstrated.},
subject = {RNS},
language = {en}
}
@article{GorboulevKutateladzeBarciszewskietal.1977,
author = {Gorboulev, Valentin G. and Kutateladze, Tamara V. and Barciszewski, Jan and Axelrod, Vladimir D.},
title = {The separation of oligonucleotides of baker's yeast valine transfer ribonucleic acid 2b by high-voltage electrophoresis on DEAE-paper and by thin-layer chromatography},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32536},
year = {1977},
abstract = {No abstract available},
language = {en}
}
@article{GorboulevAxelrodBayev1977,
author = {Gorboulev, Valentin G. and Axelrod, Vladimir D. and Bayev, Alexander A.},
title = {Primary structure of baker's yeast valine tRNA\(^{Val}_{2b}\)},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32546},
year = {1977},
abstract = {The minor form of vallne tBNA from baker's yeaat - tRNA\(^{Val}_{2b}\) - purified by column chromatography was completely digesteft with guanylo-BNase and pancreatic ENase. The products of these digestions were separated by a combination of thin-layer chromatography on cellulose and high voltage electrophoresis on DEAE-paper and then identified. The halves of tRNA Val 2b were prepared by partial digestion with pancreatic Mass and their complete guanylo-BNase and pancreatic ENase, digests were analysed. Basing on the obtained data the primary structure of baker1s yeast tRNA\(^{Val}_{2b}\) was reconstructed.},
language = {en}
}
@article{KutateladzeAxelrodGorboulevetal.1979,
author = {Kutateladze, T. V. and Axelrod, V. D. and Gorboulev, Valentin G. and Belzhelarskaya, S. N. and Vartikyan, R. V.},
title = {New procedure of high-voltage electrophoresis in polyacrylamide gel and its application to the sequencing of nucleic acids},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46927},
year = {1979},
abstract = {Fractionation of nucleic acids and their fragments with polyacrylamide gel has been widely applied in sequencing of nucleic acids. Although the conditions of electrophoresis for this purpose have previously been suggested. we have found that polyacrylamide gel electrophoresis at 5000 V (100 V/cm) is possible and effective. An apparatus consisting of a horizontal thermostated plate is used to remove the heat which was formed during the electrophoretic process. The techniques for loading samples on the horizontal thin gel and the procedure for high-voltage gel electrophoresis are described and illustrated by the fractionation of the spleen phosphodiesterase partial digest of tRNA¥~1 as well as by the RNA synthesis by RNA polymerase from E. coli with poly[d(A- T)j as template in the presence of "terminator," 3'-O-methyluridine 5'-triphosphate. This same technique was used for electrophoresis of oligonucleotides on acetylcellulose and was incorporated into a two-dimensional system which was demonstrated by fingerprinting of the guanylo-RNase digest of tRNAT'P from baker's yeast. In the third part of the article a simple technique for the electric trapping of nucleic acids or their fragments from a slab gel on a DEAE-paper sheet is presented.},
language = {en}
}
@inproceedings{VivianiLutz1979,
author = {Viviani, A. and Lutz, Werner K.},
title = {Modulation of the in vivo covalent binding of the carcinogen benzo(a)pyrene to rat liver DNA by selective induction of microsomal and nuclear aryl hydrocarbon hydroxylase activity},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-80132},
year = {1979},
abstract = {The influence of microsomal (mAHH) and nuclear (nAHH) aryl hydrocarbon hydroxylase activity on the covalent binding of t:titiated benzo(a)pyrene to rat liver DNA was evaluated in vivo. Induction ofmAHH was obtained after phenobarbitone treatment (180\% of control), which increased DNA binding to 210\%, but left the nAHH unchanged. mAHH and nAHH were slightly indilced with dieldrin (130\% and 120\%), but the binding remairred unchanged. The increasing effect of mAHlt as weil as the possibly decreasing effect of nAHH induction on the binding became obvious when the data of 11 individual rats were used to solve the equation Binding = aX(mAHH) + bX(nAHH) + c. Multiple linear regression analysis resulted in positive values for a and c, a negative value for b, and a multiple correlation coefficient R = 0.82. An influence of other enzymes involved in the metabolism of benzo(a)pyrene cannot be excluded. The Study shows clearly that the binding of a foreign compound to DNA in vivo is not only dependent on microsomal enzyme activities but also on nuclear activities even if the latter are considerably lower than those of mic'rosomes.},
subject = {DNA},
language = {en}
}
@article{AgranovskyDolyaGorboulevetal.1981,
author = {Agranovsky, A. A. and Dolya, V. V. and Gorboulev, Valentin G. and Kozlov, J. V. and Atabekov, J. G.},
title = {Aminoacylation of barley stripe mosaic virus RNA: polyadenylate-containing RNA has a 3'-terminal tyrosine-accepting structure},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32566},
year = {1981},
abstract = {Barley stripe mosaic virus (BSMV) RNA which was previously reported to contain poly(A) sequences (Agranovsky et al., 1978) can be specifically esterified with tyrosine in vitro in the presence of an aminoacyl-tRNA synthetase fraction from wheat embryos. All the three RNA components of the BSMV strain with a three-component genome (Norwich) and both RNA components of a two-component strain (Russian) can be tyrosylated. The poly(A)-containing (bound to oligo(dT)-cellulose) and poly(A)-deficient(not bound to oligo(dT)-cellulose) fractions of BSMV RNA display a similar amino acidaccepting ability. The nucleotide sequence which accepts tyrosine is coupled with the intact genomic polyadenylated BSMV RNA. The viral RNA isolated after sucrose density gradient centrifugation under drastic denaturing conditions retains its aminoacylating activity, which suggests that this activity is not due to the presence in a BSMV RNA preparation of a tyrosine tRNA associated with BSMV RNA. Inhibition of aminoacylation of the 3'-oxidized (treated with sodium metaperiodate) BSMV RNA suggests that the tyrosine-accepting structure is localized at the 3' terminus of BSMV RNA molecules. It is shown that segments of different lengths obtained upon random fragmentation can be tyrosylated. The 3'-terminal (tyrosine-accepting) poly(A)+ segments can be isolated. The shortest segments of viral RNA capable of being aminoacylated [i.e., containing both tRNA-like structure and poly(A)] consists of approximately 150-200 nucleotides. The analysis of the oligonucleotides derived from individual BSMV RNA components labeled with 32P at the 3' end revealed two types of 3'-terminal sequences different from poly(A). It is suggested that a poly(A) sequence is intercalated between a 3'-terminal tyrosineaccepting structure and the 5'-terminal portion of poly(A)+ BSMV RNA.},
language = {en}
}
@article{KozlovGorboulevKurmanovaetal.1981,
author = {Kozlov, J. V. and Gorboulev, Valentin G. and Kurmanova, A. G. and Bayev, Alexander A. and Shilov, A. A. and Zhdanov, V. M.},
title = {On the origin of the H1N1 (A/USSR/90/77) influenza virus},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32556},
year = {1981},
abstract = {The influenza virus H1N1 (the A/USSR/90/77 strain) that reappeared in 1977 after the H1N1 influenza viruses had disappeared from the human population, is compared with the A/FM/1/47 and the A/FW/1/50 influenza viruses by the method of oligonucleotide mapping of individual segments of the viral RNAs. Seven genes of the A/USSR/90/77 virus appear to be very similar to the corresponding genes of the A/FW/1/50 virus, whereas the gene coding for the M protein displays considerable homology to the corresponding gene of the A/FM/1/47 virus. The data demonstrate that the A/USSR/90/77 strain is a recombinant virus.},
language = {en}
}
@article{ArtyukovaGorboulevRodionovaetal.1985,
author = {Artyukova, E. V. and Gorboulev, Valentin G. and Rodionova, N. P. and Krylov, A. V. and Atabekov, J. G.},
title = {Comparative study of structural peculiarities and translation of potexvirus RNAs},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46985},
year = {1985},
abstract = {Structural peculiarities of the S'-end segments of genomic RNA were studied in F potato virus (F-PV) and white clover mosaic virus (WCMV). The methods of affinity chromatography on oligo(dT) cellulose and oligonucleotide mapping revealed a prolonged (up to 210 nucleotides) polyadenyl sequence at the 3'-end of F-PV RNA. A polyadenyl sequence is missing at the 3'end of WCMV RNA. A study of the translation products of WCMV and F-PV RNAs in a oe11-free protein-synthesizing system derived from rabbit reticulocytes showed that polypeptides electrophoretically comigrating with a structural protein of either virus were synthesized alongside high-molecular-weight polypeptides (M\(_r\)\(\approx\) 180-150 kdaltons).},
language = {en}
}
@article{RubtsovChernovGorboulevetal.1985,
author = {Rubtsov, P. M. and Chernov, V. G. and Gorboulev, Valentin G. and Parsadanyan, A. S. and Sverdlova, P. S. and Chupeeva, V. V. and Golova, Yu. B. and Batchikova, N. V. and Zvirblis, G. S. and Skryabin, K. G. and Bayev, A. A.},
title = {Genetic engineering of peptide hormones},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46964},
year = {1985},
abstract = {Peptide and polypeptide hormones represent an extensive group of biologically active compounds of important significance for medicine and agriculture. In recent years genetic engineering methods have been used to create strains of microorganisms synthesizing eukaryotic proteins, including hormones and their precursors. The first stage of such developments is the isolation of DNA coding the des~red product. We have accomplished the cloning of the cDNA of a number of polypeptide and peptide hormones of the pituitary of man and domestic animals. The model gene of human calcitonin has also been synthesized and cloned. The obtained genes are being used to develop methods for the microbiological synthesis of human and animal-hormones.},
language = {en}
}
@article{BoriskinDesyatskovaBogomolovaetal.1986,
author = {Boriskin, Yu S. and Desyatskova, R. G. and Bogomolova, N. N. and Gorboulev, Valentin G.},
title = {Stability of rubella virus after long-term persistence in human cell line},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46944},
year = {1986},
abstract = {Primary infection of HEp-2 cells with rubella virus resulted in non-cytophatic longterm persistent infection. During four years of persistence the virus was produced in sufficient quantities (up to 6 logs PFU/ml) and did not differ from the parental variant in its pathogenicity for BHK-21 or RK-13 cells, or hemagglutinating activity, but formed smaller plaques. Persistent virus preserved the original antigenicity as judged from reciprocal hemagglutination-inhibition or plaque reduction-neutralization tests with polyclonal antisera. Both original and persistent rubella viruses were thermoresistant (T 56° C) and sligthly temperature-sensitive. Clonal analysis revealed presence of ts-mutants among both original and persistent virus clones with different degrees of plating efficiency at 40°/34° C. RNA fingerprinting showed only minor changes in persistent rubella virus.},
language = {en}
}
@article{ZvirblisGorboulevRubtsovetal.1988,
author = {Zvirblis, G. S. and Gorboulev, Valentin G. and Rubtsov, P. M. and Chernov, B. K. and Golova, Yu. B. and Pozmogova, G. E. and Skryabin, K. G. and Bayev, A. A.},
title = {Genetic engineering of peptide hormones : III. Cloning of cDNA of porcine growth hormone and construction of gene for expression of hormone in bacteria},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46958},
year = {1988},
abstract = {Results are presented of cloning cDNA of procine growth hormone, analysis of its primary structure, and creation of a construction capable of expression of this cDNA in Esqheriahia coti cells. It is shown that in the population of mRNA coding porcine growth hormone, heterogeneity is noted which is manifested not only at the level of the nucleotide sequence, but also is reflected in the amino acid sequence of the mature hormone.},
language = {en}
}
@article{RubtsovOganessyanGorboulevetal.1988,
author = {Rubtsov, P. M. and Oganessyan, R. G. and Gorboulev, Valentin G. and Skryabin, K. G. and Bayev, A. A.},
title = {Genetic engineering of peptide hormones : II. Possible polymorphism of preprolactin in cattle. Data of molecular cloning},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46975},
year = {1988},
abstract = {Primary structure is determined of an insertion of a clone isolated from the library of hypophyseal cDNA of cattle by hybridization with a probe specific for prolactin. Analysis of nucleotide sequences showed that in the process of cloning, reorganization occurred in structure of preprolactin cDNA, including an inversion of the 5'-terminal and deletion of the central section of cDNA. Nevertheless, from structure of cDNA, nucleotide sequences can be deduced of extended 5'- and 3'-terminal sections of preprolactin mRNA in cattle with lengths of 257 and 551 nucleotide residues, respectively. When these sequences are compared to those established previously, some differences were found in primary structure. The most important of them is the presence of an additional codon which codes alanine at the position (-22) of the signal peptide. It is suggested that heterogeneity of preprolactin mRNA of cattle in the section coding the signal peptide is the result of alternative splicing, as was shown for preprolactin mRNA in rats.},
language = {en}
}
@article{TsfasmanNesmeyanovaGorboulevetal.1989,
author = {Tsfasman, I. M. and Nesmeyanova, M. A. and Gorboulev, Valentin G. and Rubtsov, P. M. and Skryabin, K. G.},
title = {Biosynthesis and secretion of bovine growth hormone in Escherichia coli under the control of the secretory vector containing a promoter and signal sequence of alkaline phosphatase gene},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-46932},
year = {1989},
abstract = {A recombinant plasmid was constructed containing the gene for bovine growth hormone joinea with the regulatory region and the region coding the signal sequence of the Escherichia coli alkaline phosphatase gene. In conditions of phosphorus starvation, which c~s derepression of alkaline phosphatase, expression was shown of the gene for bovine growth hormone, in addition to partial processing and secretion of protein into periplasm.},
language = {en}
}
@article{GorboulevAkhundovaLuziusetal.1992,
author = {Gorboulev, Valentin and Akhundova, Aida and Luzius, Heike and Fahrenholz, Falk},
title = {Molecular cloning of substance P receptor cDNA from guinea-pig uterus},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59293},
year = {1992},
abstract = {A cDNA encoding guinea-pig uterine substance P (SP) receptor has been isolated using the homology screening approach. Northern blot analysis reveals that the corresponding mRNA, of approx. 4.8 kb, is expressed in all tissues tested, but predominantly in the uteri of non-pregnant animals; during pregnancy its expression is reduced. The guinea-pig SP receptor was expressed in COS-7 cells and demonstrated relative Iigand affinity in the order: SP >> neurokinin A > neurokinin B.},
subject = {Biologie},
language = {en}
}
@article{GorboulevAkhundovaBuechneretal.1992,
author = {Gorboulev, Valentin and Akhundova, Aida and B{\"u}chner, Hubert and Fahrenholz, Falk},
title = {Molecular cloning of a new bombesin receptor subtype expressed in uterus during pregnancy},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59304},
year = {1992},
abstract = {The homology screening approach has been used to clone a new member of the guanine-nucleotidebinding-protein-coupled receptor superfamily from guinea pig uterus. The cloned cDNA encodes a 399-amino-acid protein and shows the highest amino acid similarity to members of the bombesin receptor family; 52\% and 47\% similarity to the gastrin-releasing-peptide (GRP) receptor and the neuromedin-B receptor, respectively. Bindingexperiments with the stably transfected LLC-PK1 cell line expressing the new receptor protein confmned the bombesin-like nature of the cloned receptor. The relative order ofligand affinity, GRP = neuromedin C >> neuromedin B, suggests that the cloned cDNA represents the GRP subtype rather than the neuromedin-B subtype of bombesin receptors. Northern-blot analysis of mRNA species from several guinea-pig tissues showed that the mRNA for the new bombesin receptor subtype is expressed mainly in uteri of pregnant animals.},
subject = {Biologie},
language = {en}
}
@article{GorboulevBuechnerAkhundovaetal.1993,
author = {Gorboulev, Valentin and B{\"u}chner, Hubert and Akhundova, Aida and Fahrenholz, Falk},
title = {Molecular cloning and functional characterization of V2 [8-Iysine] vasopressin and oxytocin receptors from a pig kidney cell line (LLC-PK1)},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59311},
year = {1993},
abstract = {No abstract available},
subject = {Biologie},
language = {en}
}
@article{GruendemannGorboulevGambaryanetal.1994,
author = {Gr{\"u}ndemann, Dirk and Gorboulev, Valentin and Gambaryan, Stepan and Veyhl, Maike and Koepsell, Hermann},
title = {Drug excretion mediated by a new prototype of polyspecific transporter},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59327},
year = {1994},
abstract = {CATIO~IC drugs of different types and structures (antihistaminics, antiarrhythmics, sedatives, opiates, cytostatics and antibiotics, for example) are excreted in mammals by epithelial cells of the renal proximal tubules and by hepatocytes in the liver1-4. In the proximal tubules, two functionally disparate transport systems are involved which are localized in the basolateral and luminal plasma membrane and are different from the previously identified neuronal monoamine transporters and A TP-dependent multidrug exporting proteins1-3,5-12. Here we report the isolation of a complementary DNA from rat kidney that encodes a 556-amino-acid membrane protein, OCT1, which has the functional characteristics of organic cation uptake over the basolateral membrane of renal proximal tubules and of organic cation uptake into hepatocytes. OCTl is not homologous to any other known protein and is found in kidney, liver and intestine. As OCTl translocates hydrophobic and hydrophilic organic cations of different structures, it is considered to be a new prolotype of polyspecific transporters that are important for drug elimination.},
subject = {Biologie},
language = {en}
}
@article{GorboulevAkhundovaGrzeschiketal.1994,
author = {Gorboulev, Valentin G. and Akhundova, Aida and Grzeschik, K.-H. and Fahrenholz, Falk},
title = {Organization and chromosomal localization of the gene for the human bombesin receptor subtype expressed in pregnant uterus},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32572},
year = {1994},
abstract = {The gene encoding the human homologue of the guinea pig uterine bombesm receptor [( 1992) Eur. J. Biochem. 208,405] was isolated from a genomic lambda library by the PCR/homology screening approach. The gene spans more than 4 kb and consists of 3 exons and 2 introns. The deduced amino acid sequence shows about 86\% identity to that of guinea pig bombesin receptor. This subtype of bombesin receptor is expressed in the pregnant uterus and in two human tumour cell lines, T47D (ductal breast carcinoma) and A431 (epidermal carcinoma). PCR analysis of genomic DNA from human-mouse cell hybrids allows the cloned gene to be localized to the region q26q28 on chromosome X.},
language = {en}
}
@phdthesis{Arndt2000,
author = {Arndt, Petra},
title = {Klonierung und funktionelle Charakterisierung von organischen Kationentransportern aus der Rattenniere},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-793},
school = {Universit{\"a}t W{\"u}rzburg},
year = {2000},
abstract = {Der organische Kationentransport im proximalen Tubulus der Niere spielt eine wichtige Rolle bei der Aufrechterhaltung der Hom{\"o}ostase der K{\"o}rperfl{\"u}ssigkeiten und der Ausschleusung von toxischen organischen Kationen. Der Transport von organischen Kationen wird an der B{\"u}rstensaummembran durch den H+/organische Kationen-Austauscher vermittelt, w{\"a}hrend bei dem Transport von organischen Kationen an der basolateralen Membran das nach innen gerichtete negative Membranpotential eine treibende Kraft darstellt. Durch Expressionsklonierung wurde der erste organische Kationentransporter, rOCT1, aus der Rattenniere isoliert. Kurz darauf wurde im Rahmen dieser Arbeit ein zweiter organischer Kationentransporter ebenfalls aus der Ratenniere kloniert. rOCT2 besteht aus 593 Aminos{\"a}uren und besitzt 12 putative Transmembrandom{\"a}nen. Zum funktionellen Vergleich zwischen rOCT1 und rOCT2 wurde das Oozytenexpressionssystem verwendet. In der vorliegenden Arbeit wurde ein pharmakologisches Profil von rOCT2 erstellt. Das Substratsprektrum von rOCT2 ist dem von rOCT1 sehr {\"a}hnlich. Die Affinit{\"a}ten von rOCT2 gegen{\"u}ber verschiedenen Substanzen wurden direkt mit denen von rOCT1 verglichen. Einerseits fanden wir bei einigen Substraten Unterschiede in den Km- und Vmax-Werten, aber andererseits auch viele {\"A}hnlichkeiten zwischen beiden Transportern. Anionen (z. B. p-Aminohippurat) wurden als neue Gruppe von Inhibitoren f{\"u}r den durch rOCT1- und rOCT2-vermittelten Transport identifiziert. Die Potentialdifferenz ist die treibende Kraft des rOCT1- und rOCT2-vermittelten Transportes. Wir konnten potentialabh{\"a}ngige Ver{\"a}nderungen der Km-Werte von Cholin-induzierten Einw{\"a}rtsstr{\"o}men zeigen. Bei dem Austausch von Na+-Ionen gegen K+-Ionen im Reaktionspuffer wurde die Aufnahme von Cholin und MPP durch rOCT2 erniedrigt. Der bidirektionale Transport von MPP wurde gezeigt und trans-Stimulationsexperimente f{\"u}r MPP-Influx und MPP-Efflux durchgef{\"u}hrt, um die Asymmetrie des Transporters zu studieren. Dar{\"u}berhinaus wurde in der vorliegenden Arbeit die Interaktion von verschiedenen Substraten mit rOCT1 und rOCT2 untersucht und ein kompetitver und nicht-kompetitiver Hemmtyp bei der TEA-Aufnahme gefunden.},
subject = {Ratte},
language = {de}
}
@phdthesis{Kuehlkamp2001,
author = {K{\"u}hlkamp, Thomas},
title = {Der plasmamembran assoziierte Transportregulator RS1 bindet Ubiquitin und gelangt in den Zellkern},
url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-1179507},
school = {Universit{\"a}t W{\"u}rzburg},
year = {2001},
abstract = {Die vorliegende Arbeit liefert wichtige Erkenntnisse {\"u}ber die subzellul{\"a}re Verteilung und die Funktion des RS1-Proteins vom Schwein (pRS1), einem Regulator von Plasmamembran-transportern. Das gr{\"u}n fluoreszierende Protein (GFP) wurde mit pRS1 fusioniert und in LLC-PK1 Zellen exprimiert. Das GFP-pRS1 Fusionsprodukt (96 kD) konnte an der Plasmamembran, im Zytosol und im Zellkern entdeckt werden. Bei GFP-Fusion mit trunkierten pRS1-Proteinen zeigte sich, dass der C-Terminus die Kernlokalisierung beeinflusst. Dagegen wurde die Kernlokalisierung durch eine Trunkierung des N-Terminus nicht gest{\"o}rt. Im C-Terminus des pRS1 konnte von AS 579 bis 616 eine Ubiquitin associated domain (UBA) identifiziert werden, die auch in den anderen bisher bekannten RS1-Proteinen aus Mensch, Kaninchen und Maus konserviert vorliegt. Eine Ubiquitin-Affinit{\"a}tschromatographie zeigte, dass das pRS1-Protein Ubiquitin auf nicht kovalente Weise bindet. Nach der Trunkierung der UBA-Dom{\"a}ne war keine Wechselwirkung des pRS1-Proteins mit Ubiquitin mehr feststellbar. Ein konserviertes Di-Leucin-Endozytose-Motiv (pRS1 AS 366/67) deutet eine Funktion des pRS1-Proteins bei der Internalisierung von Plasmamembranproteinen an. Deshalb wurde das Endozytoseverhalten von pRS1 {\"u}berexprimierenden LLC-PK1 Zellen untersucht, wobei sich zeigte, dass diese Zellen eine deutlich h{\"o}here Aufnahme des Endozytosefarbstoffes RH 414 aufwiesen als Zellen, die pRS1 nicht {\"u}berexprimierten. Die in dieser Arbeit gesammelten Daten zum RS1-Protein wurden zusammen mit fr{\"u}her erhobenen Ergebnissen zum RS1-Protein im Rahmen eines Modells zusammengefasst. In diesem hypothetischen Modell wird angenommen, dass RS1 ein Adapterprotein ist, welches die ubiquitinabh{\"a}ngige Endozytose von Plasmamembrantransportern vermittelt und als Signalmolek{\"u}l in den Zellkern gelangen kann, wo es an der Transcriptionsrepression des SGLT1 beteiligt ist.},
subject = {Ubiquitin},
language = {de}
}