@article{StepulaKoenigWangetal.2020,
  author    = {Stepula, Elzbieta and K{\"o}nig, Matthias and Wang, Xin-Ping and Levermann, Janina and Schimming, Tobias and Kasimir-Bauer, Sabine and Schilling, Bastian and Schl{\"u}cker, Sebastian},
  title     = {Localization of PD-L1 on single cancer cells by iSERS microscopy with Au/Au core/satellite nanoparticles},
  series = {Journal of Biophotonics},
  volume    = {13},
  journal   = {Journal of Biophotonics},
  number    = {3},
  doi       = {10.1002/jbio.201960034},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-212655},
  year      = {2020},
  abstract  = {Programmed cell death-ligand 1 (PD-L1) is an important predictive biomarker. The detection of PD-L1 can be crucial for patients with advanced cancer where the use of immunotherapy is considered. Here, we demonstrate the use of immuno-SERS microscopy (iSERS) for localizing PD-L1 on single cancer SkBr-3 cells. A central advantage of iSERS is that the disturbing autofluorescence from cells and tissues can be efficiently minimized by red to near-infrared laser excitation. In this study we employed Au/Au core/satellite nanoparticles as SERS nanotags because of their remarkable signal brightness and colloidal stability upon red laser excitation. False-color iSERS images of the positive and negative controls clearly reveal the specific localization of PD-L1 with SERS nanotag-labeled antibodies.},
  language  = {en}
}