@article{BinasBessiSchwalbe2020,
  author    = {Binas, Oliver and Bessi, Irene and Schwalbe, Harald},
  title     = {Structure Validation of G-Rich RNAs in Noncoding Regions of the Human Genome},
  series = {ChemBioChem},
  volume    = {21},
  journal   = {ChemBioChem},
  number    = {11},
  doi       = {10.1002/cbic.201900696},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-214892},
  pages     = {1656 -- 1663},
  year      = {2020},
  abstract  = {We present the rapid biophysical characterization of six previously reported putative G-quadruplex-forming RNAs from the 5′-untranslated region (5′-UTR) of silvestrol-sensitive transcripts for investigation of their secondary structures. By NMR and CD spectroscopic analysis, we found that only a single sequence—[AGG]\(_{2}\)[CGG]\(_{2}\)C—folds into a single well-defined G-quadruplex structure. Sequences with longer poly-G strands form unspecific aggregates, whereas CGG-repeat-containing sequences exhibit a temperature-dependent equilibrium between a hairpin and a G-quadruplex structure. The applied experimental strategy is fast and provides robust readout for G-quadruplex-forming capacities of RNA oligomers.},
  language  = {en}
}