@article{GorboulevAkhundovaBuechneretal.1992, author = {Gorboulev, Valentin and Akhundova, Aida and B{\"u}chner, Hubert and Fahrenholz, Falk}, title = {Molecular cloning of a new bombesin receptor subtype expressed in uterus during pregnancy}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59304}, year = {1992}, abstract = {The homology screening approach has been used to clone a new member of the guanine-nucleotidebinding-protein-coupled receptor superfamily from guinea pig uterus. The cloned cDNA encodes a 399-amino-acid protein and shows the highest amino acid similarity to members of the bombesin receptor family; 52\% and 47\% similarity to the gastrin-releasing-peptide (GRP) receptor and the neuromedin-B receptor, respectively. Bindingexperiments with the stably transfected LLC-PK1 cell line expressing the new receptor protein confmned the bombesin-like nature of the cloned receptor. The relative order ofligand affinity, GRP = neuromedin C >> neuromedin B, suggests that the cloned cDNA represents the GRP subtype rather than the neuromedin-B subtype of bombesin receptors. Northern-blot analysis of mRNA species from several guinea-pig tissues showed that the mRNA for the new bombesin receptor subtype is expressed mainly in uteri of pregnant animals.}, subject = {Biologie}, language = {en} } @article{GorboulevBuechnerAkhundovaetal.1993, author = {Gorboulev, Valentin and B{\"u}chner, Hubert and Akhundova, Aida and Fahrenholz, Falk}, title = {Molecular cloning and functional characterization of V2 [8-Iysine] vasopressin and oxytocin receptors from a pig kidney cell line (LLC-PK1)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59311}, year = {1993}, abstract = {No abstract available}, subject = {Biologie}, language = {en} }