@article{WohlfarthSchmitteckertHaertleetal.2017,
  author    = {Wohlfarth, Carolin and Schmitteckert, Stefanie and H{\"a}rtle, Janina D. and Houghton, Lesley A. and Dweep, Harsh and Fortea, Marina and Assadi, Ghazaleh and Braun, Alexander and Mederer, Tanja and P{\"o}hner, Sarina and Becker, Philip P. and Fischer, Christine and Granzow, Martin and M{\"o}nnikes, Hubert and Mayer, Emeran A. and Sayuk, Gregory and Boeckxstaens, Guy and Wouters, Mira M. and Simr{\´e}n, Magnus and Lindberg, Greger and Ohlsson, Bodil and Schmidt, Peter Thelin and Dlugosz, Aldona and Agreus, Lars and Andreasson, Anna and D'Amato, Mauro and Burwinkel, Barbara and Bermejo, Justo Lorenzo and R{\"o}th, Ralph and Lasitschka, Felix and Vicario, Maria and Metzger, Marco and Santos, Javier and Rappold, Gudrun A. and Martinez, Cristina and Niesler, Beate},
  title     = {miR-16 and miR-103 impact 5-HT4 receptor signalling and correlate with symptom profile in irritable bowel syndrome},
  series = {Scientific Reports},
  volume    = {7},
  journal   = {Scientific Reports},
  doi       = {10.1038/s41598-017-13982-0},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-173478},
  year      = {2017},
  abstract  = {Irritable bowel syndrome (IBS) is a gut-brain disorder involving alterations in intestinal sensitivity and motility. Serotonin 5-HT4 receptors are promising candidates in IBS pathophysiology since they regulate gut motor function and stool consistency, and targeted 5-HT4R selective drug intervention has been proven beneficial in subgroups of patients. We identified a single nucleotide polymorphism (SNP) (rs201253747) c.*61 T > C within the 5-HT4 receptor gene \(HTR4\) to be predominantly present in diarrhoea-IBS patients (IBS-D). It affects a binding site for the miR-16 family and miR-103/miR-107 within the isoforms \({HTR4b/i}\) and putatively impairs \(HTR4\) expression. Subsequent miRNA profiling revealed downregulation of miR-16 and miR-103 in the jejunum of IBS-D patients correlating with symptoms. \(In\) \(vitro\) assays confirmed expression regulation via three 3′UTR binding sites. The novel isoform \(HTR4b\_2\) lacking two of the three miRNA binding sites escapes miR-16/103/107 regulationin SNP carriers. We provide the first evidence that \(HTR4\) expression is fine-tuned by miRNAs, and that this regulation is impaired either by the SNP c.*61 T > C or bydiminished levels of miR-16 and miR-103 suggesting that \(HTR4\) might be involved in the development of IBS-D.},
  language  = {en}
}
@article{StrasserSchrauthDembskietal.2017,
  author    = {Straßer, Marion and Schrauth, Joachim H. X. and Dembski, Sofia and Haddad, Daniel and Ahrens, Bernd and Schweizer, Stefan and Christ, Bastian and Cubukova, Alevtina and Metzger, Marco and Walles, Heike and Jakob, Peter M. and Sextl, Gerhard},
  title     = {Calcium fluoride based multifunctional nanoparticles for multimodal imaging},
  series = {Beilstein Journal of Nanotechnology},
  volume    = {8},
  journal   = {Beilstein Journal of Nanotechnology},
  doi       = {10.3762/bjnano.8.148},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-170657},
  pages     = {1484-1493},
  year      = {2017},
  abstract  = {New multifunctional nanoparticles (NPs) that can be used as contrast agents (CA) in different imaging techniques, such as photoluminescence (PL) microscopy and magnetic resonance imaging (MRI), open new possibilities for medical imaging, e.g., in the fields of diagnostics or tissue characterization in regenerative medicine. The focus of this study is on the synthesis and characterization of CaF\(_{2}\):(Tb\(^{3+}\),Gd\(^{3+}\)) NPs. Fabricated in a wet-chemical procedure, the spherical NPs with a diameter of 5-10 nm show a crystalline structure. Simultaneous doping of the NPs with different lanthanide ions, leading to paramagnetism and fluorescence, makes them suitable for MR and PL imaging. Owing to the Gd\(^{3+}\) ions on the surface, the NPs reduce the MR T\(_{1}\) relaxation time constant as a function of their concentration. Thus, the NPs can be used as a MRI CA with a mean relaxivity of about r = 0.471 mL·mg\(^{-1}\)·s\(^{-1}\). Repeated MRI examinations of four different batches prove the reproducibility of the NP synthesis and determine the long-term stability of the CAs. No cytotoxicity of NP concentrations between 0.5 and 1 mg·mL\(^{-1}\) was observed after exposure to human dermal fibroblasts over 24 h. Overall this study shows, that the CaF\(_{2}\):(Tb\(^{3+}\),Gd\(^{3+}\)) NPs are suitable for medical imaging.},
  language  = {en}
}
@article{AppeltMenzelCubukovaGuentheretal.2017,
  author    = {Appelt-Menzel, Antje and Cubukova, Alevtina and G{\"u}nther, Katharina and Edenhofer, Frank and Piontek, J{\"o}rg and Krause, Gerd and St{\"u}ber, Tanja and Walles, Heike and Neuhaus, Winfried and Metzger, Marco},
  title     = {Establishment of a Human Blood-Brain Barrier Co-culture Model Mimicking the Neurovascular Unit Using Induced Pluri- and Multipotent Stem Cells},
  series = {Stem Cell Reports},
  volume    = {8},
  journal   = {Stem Cell Reports},
  number    = {4},
  doi       = {10.1016/j.stemcr.2017.02.021},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-170982},
  pages     = {894-906},
  year      = {2017},
  abstract  = {In vitro models of the human blood-brain barrier (BBB) are highly desirable for drug development. This study aims to analyze a set of ten different BBB culture models based on primary cells, human induced pluripotent stem cells (hiPSCs), and multipotent fetal neural stem cells (fNSCs). We systematically investigated the impact of astrocytes, pericytes, and NSCs on hiPSC-derived BBB endothelial cell function and gene expression. The quadruple culture models, based on these four cell types, achieved BBB characteristics including transendothelial electrical resistance (TEER) up to 2,500 Ω cm\(^{2}\) and distinct upregulation of typical BBB genes. A complex in vivo-like tight junction (TJ) network was detected by freeze-fracture and transmission electron microscopy. Treatment with claudin-specific TJ modulators caused TEER decrease, confirming the relevant role of claudin subtypes for paracellular tightness. Drug permeability tests with reference substances were performed and confirmed the suitability of the models for drug transport studies.},
  language  = {en}
}