@article{BinasBessiSchwalbe2020,
  author    = {Binas, Oliver and Bessi, Irene and Schwalbe, Harald},
  title     = {Structure Validation of G-Rich RNAs in Noncoding Regions of the Human Genome},
  series = {ChemBioChem},
  volume    = {21},
  journal   = {ChemBioChem},
  number    = {11},
  doi       = {10.1002/cbic.201900696},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-214892},
  pages     = {1656 -- 1663},
  year      = {2020},
  abstract  = {We present the rapid biophysical characterization of six previously reported putative G-quadruplex-forming RNAs from the 5′-untranslated region (5′-UTR) of silvestrol-sensitive transcripts for investigation of their secondary structures. By NMR and CD spectroscopic analysis, we found that only a single sequence—[AGG]\(_{2}\)[CGG]\(_{2}\)C—folds into a single well-defined G-quadruplex structure. Sequences with longer poly-G strands form unspecific aggregates, whereas CGG-repeat-containing sequences exhibit a temperature-dependent equilibrium between a hairpin and a G-quadruplex structure. The applied experimental strategy is fast and provides robust readout for G-quadruplex-forming capacities of RNA oligomers.},
  language  = {en}
}
@article{MuellerBessiRichteretal.2021,
  author    = {M{\"u}ller, Diana and Bessi, Irene and Richter, Christian and Schwalbe, Harald},
  title     = {The Folding Landscapes of Human Telomeric RNA and DNA G-Quadruplexes are Markedly Different},
  series = {Angewandte Chemie International Edition},
  volume    = {60},
  journal   = {Angewandte Chemie International Edition},
  number    = {19},
  doi       = {10.1002/anie.202100280},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-238917},
  pages     = {10895 -- 10901},
  year      = {2021},
  abstract  = {We investigated the folding kinetics of G-quadruplex (G4) structures by comparing the K\(^{+}\)-induced folding of an RNA G4 derived from the human telomeric repeat-containing RNA (TERRA25) with a sequence homologous DNA G4 (wtTel25) using CD spectroscopy and real-time NMR spectroscopy. While DNA G4 folding is biphasic, reveals kinetic partitioning and involves kinetically favoured off-pathway intermediates, RNA G4 folding is faster and monophasic. The differences in kinetics are correlated to the differences in the folded conformations of RNA vs. DNA G4s, in particular with regard to the conformation around the glycosidic torsion angle χ that uniformly adopts anti conformations for RNA G4s and both, syn and anti conformation for DNA G4s. Modified DNA G4s with \(^{19}\)F bound to C2′ in arabino configuration adopt exclusively anti conformations for χ. These fluoro-modified DNA (antiTel25) reveal faster folding kinetics and monomorphic conformations similar to RNA G4s, suggesting the correlation between folding kinetics and pathways with differences in χ angle preferences in DNA and RNA, respectively.},
  language  = {en}
}