@article{SteinmetzgerBaeuerleinHoebartner2020,
  author    = {Steinmetzger, Christian and B{\"a}uerlein, Carmen and H{\"o}bartner, Claudia},
  title     = {Supramolecular fluorescence resonance energy transfer in nucleobase-modified fluorogenic RNA aptamers},
  series = {Angewandte Chemie, International Edition},
  volume    = {59},
  journal   = {Angewandte Chemie, International Edition},
  doi       = {10.1002/anie.201916707},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-203084},
  pages     = {6760-6764},
  year      = {2020},
  abstract  = {RNA aptamers form compact tertiary structures and bind their ligands in specific binding sites. Fluorescence-based strategies reveal information on structure and dynamics of RNA aptamers. Here we report the incorporation of the universal emissive nucleobase analog 4-cyanoindole into the fluorogenic RNA aptamer Chili, and its application as a donor for supramolecular FRET to bound ligands DMHBI+ or DMHBO+. The photophysical properties of the new nucleobase-ligand-FRET pair revealed structural restraints for the overall RNA aptamer organization and identified nucleotide positions suitable for FRET-based readout of ligand binding. This strategy is generally suitable for binding site mapping and may also be applied for responsive aptamer devices.},
  language  = {en}
}