@article{TimmermansvanderTolTimmermansetal.2015,
  author    = {Timmermans, Wim J. and van der Tol, Christiaan and Timmermans, Joris and Ucer, Murat and Chen, Xuelong and Alonso, Luis and Moreno, Jose and Carrara, Arnaud and Lopez, Ramon and Fernando de la Cruz, Tercero and Corcoles, Horacio L. and de Miguel, Eduardo and Sanchez, Jose A. G. and Perez, Irene and Belen, Perez and Munoz, Juan-Carlos J. and Skokovic, Drazen and Sobrino, Jose and Soria, Guillem and MacArthur, Alasdair and Vescovo, Loris and Reusen, Ils and Andreu, Ana and Burkart, Andreas and Cilia, Chiara and Contreras, Sergio and Corbari, Chiara and Calleja, Javier F. and Guzinski, Radoslaw and Hellmann, Christine and Herrmann, Ittai and Kerr, Gregoire and Lazar, Adina-Laura and Leutner, Benjamin and Mendiguren, Gorka and Nasilowska, Sylwia and Nieto, Hector and Pachego-Labrador, Javier and Pulanekar, Survana and Raj, Rahul and Schikling, Anke and Siegmann, Bastian and von Bueren, Stefanie and Su, Zhongbo (Bob)},
  title     = {An Overview of the Regional Experiments for Land-atmosphere Exchanges 2012 (REFLEX 2012) Campaign},
  series = {Acta Geophysica},
  volume    = {63},
  journal   = {Acta Geophysica},
  number    = {6},
  doi       = {10.2478/s11600-014-0254-1},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-136491},
  pages     = {1465-1484},
  year      = {2015},
  abstract  = {The REFLEX 2012 campaign was initiated as part of a training course on the organization of an airborne campaign to support advancement of the understanding of land-atmosphere interaction processes. This article describes the campaign, its objectives and observations, remote as well as in situ. The observations took place at the experimental Las Tiesas farm in an agricultural area in the south of Spain. During the period of ten days, measurements were made to capture the main processes controlling the local and regional land-atmosphere exchanges. Apart from multi-temporal, multi-directional and multi-spatial space-borne and airborne observations, measurements of the local meteorology, energy fluxes, soil temperature profiles, soil moisture profiles, surface temperature, canopy structure as well as leaf-level measurements were carried out. Additional thermo-dynamical monitoring took place at selected sites. After presenting the different types of measurements, some examples are given to illustrate the potential of the observations made.},
  language  = {en}
}
@article{KuhnGrippFliederetal.2015,
  author    = {Kuhn, Joachim and Gripp, Tatjana and Flieder, Tobias and Dittrich, Marcus and Hendig, Doris and Busse, Jessica and Knabbe, Cornelius and Birschmann, Ingvild},
  title     = {UPLC-MRM Mass Spectrometry Method for Measurement of the Coagulation Inhibitors Dabigatran and Rivaroxaban in Human Plasma and Its Comparison with Functional Assays},
  series = {PLOS ONE},
  volume    = {10},
  journal   = {PLOS ONE},
  number    = {12},
  doi       = {10.1371/journal.pone.0145478},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-136023},
  pages     = {e0145478},
  year      = {2015},
  abstract  = {Introduction The fast, precise, and accurate measurement of the new generation of oral anticoagulants such as dabigatran and rivaroxaban in patients' plasma my provide important information in different clinical circumstances such as in the case of suspicion of overdose, when patients switch from existing oral anticoagulant, in patients with hepatic or renal impairment, by concomitant use of interaction drugs, or to assess anticoagulant concentration in patients' blood before major surgery. Methods Here, we describe a quick and precise method to measure the coagulation inhibitors dabigatran and rivaroxaban using ultra-performance liquid chromatography electrospray ionization-tandem mass spectrometry in multiple reactions monitoring (MRM) mode (UPLC-MRM MS). Internal standards (ISs) were added to the sample and after protein precipitation; the sample was separated on a reverse phase column. After ionization of the analytes the ions were detected using electrospray ionization-tandem mass spectrometry. Run time was 2.5 minutes per injection. Ion suppression was characterized by means of post-column infusion. Results The calibration curves of dabigatran and rivaroxaban were linear over the working range between 0.8 and 800 mu g/L (r > 0.99). Limits of detection (LOD) in the plasma matrix were 0.21 mu g/L for dabigatran and 0.34 mu g/L for rivaroxaban, and lower limits of quantification (LLOQ) in the plasma matrix were 0.46 mu g/L for dabigatran and 0.54 mu g/L for rivaroxaban. The intraassay coefficients of variation (CVs) for dabigatran and rivaroxaban were < 4\% and 6\%; respectively, the interassay CVs were < 6\% for dabigatran and < 9\% for rivaroxaban. Inaccuracy was < 5\% for both substances. The mean recovery was 104.5\% (range 83.8-113.0\%) for dabigatran and 87.0\%(range 73.6-105.4\%) for rivaroxaban. No significant ion suppressions were detected at the elution times of dabigatran or rivaroxaban. Both coagulation inhibitors were stable in citrate plasma at -20 degrees C, 4 degrees C and even at RT for at least one week. A method comparison between our UPLC-MRM MS method, the commercially available automated Direct Thrombin Inhibitor assay (DTI assay) for dabigatran measurement from CoaChrom Diagnostica, as well as the automated anti-Xa assay for rivaroxaban measurement from Chromogenix both performed by ACL-TOP showed a high degree of correlation. However, UPLC-MRM MS measurement of dabigatran and rivaroxaban has a much better selectivity than classical functional assays measuring activities of various coagulation factors which are susceptible to interference by other coagulant drugs. Conclusions Overall, we developed and validated a sensitive and specific UPLC-MRM MS assay for the quick and specific measurement of dabigatran and rivaroxaban in human plasma.},
  language  = {en}
}