@article{GrausKonradBemmetal.2018,
  author    = {Graus, Dorothea and Konrad, Kai R. and Bemm, Felix and Nebioglu, Meliha G{\"o}rkem Patir and Lorey, Christian and Duscha, Kerstin and G{\"u}thoff, Tilman and Herrmann, Johannes and Ferjani, Ali and Cuin, Tracey Ann and Roelfsema, M. Rob G. and Schumacher, Karin and Neuhaus, H. Ekkehard and Marten, Irene and Hedrich, Rainer},
  title     = {High V-PPase activity is beneficial under high salt loads, but detrimental without salinity},
  series = {New Phytologist},
  volume    = {219},
  journal   = {New Phytologist},
  doi       = {10.1111/nph.15280},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-227553},
  pages     = {1421-1432},
  year      = {2018},
  abstract  = {The membrane-bound proton-pumping pyrophosphatase (V-PPase), together with the V-type H+-ATPase, generates the proton motive force that drives vacuolar membrane solute transport. Transgenic plants constitutively overexpressing V-PPases were shown to have improved salinity tolerance, but the relative impact of increasing PPi hydrolysis and proton-pumping functions has yet to be dissected. For a better understanding of the molecular processes underlying V-PPase-dependent salt tolerance, we transiently overexpressed the pyrophosphate-driven proton pump (NbVHP) in Nicotiana benthamiana leaves and studied its functional properties in relation to salt treatment by primarily using patch-clamp, impalement electrodes and pH imaging. NbVHP overexpression led to higher vacuolar proton currents and vacuolar acidification. After 3 d in salt-untreated conditions, V-PPase-overexpressing leaves showed a drop in photosynthetic capacity, plasma membrane depolarization and eventual leaf necrosis. Salt, however, rescued NbVHP-hyperactive cells from cell death. Furthermore, a salt-induced rise in V-PPase but not of V-ATPase pump currents was detected in nontransformed plants. The results indicate that under normal growth conditions, plants need to regulate the V-PPase pump activity to avoid hyperactivity and its negative feedback on cell viability. Nonetheless, V-PPase proton pump function becomes increasingly important under salt stress for generating the pH gradient necessary for vacuolar proton-coupled Na+ sequestration.},
  language  = {en}
}
@article{ThurowKrischkeMuelleretal.2020,
  author    = {Thurow, Corinna and Krischke, Markus and Mueller, Martin J. and Gatz, Christiane},
  title     = {Induction of jasmonoyl-isoleucine (JA-Ile)-dependent JASMONATE ZIM-DOMAIN (JAZ) genes in NaCl-treated Arabidopsis thaliana roots can occur at very low JA-Ile levels and in the absence of the JA/JA-Ile transporter JAT1/AtABCG16},
  series = {Plants},
  volume    = {9},
  journal   = {Plants},
  number    = {12},
  issn      = {2223-7747},
  doi       = {10.3390/plants9121635},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-219382},
  year      = {2020},
  abstract  = {The plant hormone jasmonoyl-isoleucine (JA-Ile) is an important regulator of plant growth and defense in response to various biotic and abiotic stress cues. Under our experimental conditions, JA-Ile levels increased approximately seven-fold in NaCl-treated Arabidopsis thaliana roots. Although these levels were around 1000-fold lower than in wounded leaves, genes of the JA-Ile signaling pathway were induced by a factor of 100 or more. Induction was severely compromised in plants lacking the JA-Ile receptor CORONATINE INSENSITIVE 1 or enzymes required for JA-Ile biosynthesis. To explain efficient gene expression at very low JA-Ile levels, we hypothesized that salt-induced expression of the JA/JA-Ile transporter JAT1/AtABCG16 would lead to increased nuclear levels of JA-Ile. However, mutant plants with different jat1 alleles were similar to wild-type ones with respect to salt-induced gene expression. The mechanism that allows COI1-dependent gene expression at very low JA-Ile levels remains to be elucidated.},
  language  = {en}
}