@article{KoenigRammeFaustetal.2022,
  author    = {Koenig, Leopold and Ramme, Anja Patricia and Faust, Daniel and Mayer, Manuela and Fl{\"o}tke, Tobias and Gerhartl, Anna and Brachner, Andreas and Neuhaus, Winfried and Appelt-Menzel, Antje and Metzger, Marco and Marx, Uwe and Dehne, Eva-Maria},
  title     = {A human stem cell-derived brain-liver chip for assessing blood-brain-barrier permeation of pharmaceutical drugs},
  series = {Cells},
  volume    = {11},
  journal   = {Cells},
  number    = {20},
  issn      = {2073-4409},
  doi       = {10.3390/cells11203295},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-290375},
  year      = {2022},
  abstract  = {Significant advancements in the field of preclinical in vitro blood-brain barrier (BBB) models have been achieved in recent years, by developing monolayer-based culture systems towards complex multi-cellular assays. The coupling of those models with other relevant organoid systems to integrate the investigation of blood-brain barrier permeation in the larger picture of drug distribution and metabolization is still missing. Here, we report for the first time the combination of a human induced pluripotent stem cell (hiPSC)-derived blood-brain barrier model with a cortical brain and a liver spheroid model from the same donor in a closed microfluidic system (MPS). The two model compounds atenolol and propranolol were used to measure permeation at the blood-brain barrier and to assess metabolization. Both substances showed an in vivo-like permeation behavior and were metabolized in vitro. Therefore, the novel multi-organ system enabled not only the measurement of parent compound concentrations but also of metabolite distribution at the blood-brain barrier.},
  language  = {en}
}