@article{KnappHackerJarchauetal.1986,
  author    = {Knapp, S. and Hacker, J{\"o}rg and Jarchau, T. and Goebel, W},
  title     = {Large, Unstable Inserts in the Chromosome Affect Virulence Properties Of Uropathogenic Escherichia coli 06 Strain 536},
  url       = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59402},
  year      = {1986},
  abstract  = {The hemolytic, uropathogenic Escherichia coli 536 (06:K15:H31) contains two inserts in its chromosome (insert I and insert II), both of which carried hly genes, were rather unstable, and were deleted spontaneously with a frequen~y of 10-3 to 10-4• These inserts were not found in the chromosome of two nonhemolytic E. coli strains, whereas the chromosomal ~equences adjacent to these inserts appeared tobe again homologous in the uropathogenic and two other E. co{\"u} strains. Insert I was 75 kilobases in size and was ftanked at both ends by 16 base pairs (bp) (TTCGACTCCTGTGATC) which were arranged in direct orientation. For insert I it was demonstrated that deletion occurred by recombination between the two 16-bp ftanking sequences, since mutants lacking this insert still carried a single copy of the 16-bp sequence in the chromosome. 8oth inserts contained a functional hemolysin determinant. However, the loss of the inserts not only atfected the hemolytic phenotype bot led to a considerable reduction in serum resistance and the loss of mannose-resistant hemagglutination, caused by the presence of S-type funbriae (sja). lt is shown that the Sfa-negative phenotype is due to a block in transcription of the sfa genes. Mutants of strain 536 which lacked both inserts were entirely avirulent when tested in several animal model systems.},
  subject      = {Infektionsbiologie},
  language  = {en}
}