@article{BrehmHaasGoebeletal.1992, author = {Brehm, Klaus and Haas, Albert and Goebel, Werner and Kreft, J{\"u}rgen}, title = {A gene encoding a superoxide dismutase of the facultative intracellular bacterium Listeria monocytogenes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60515}, year = {1992}, abstract = {A gene (Imsod) encoding superoxide dismutase (SOD; EC 1.15.1.1) of the facultative intracellular pathogen, Listeria monocytogenes, was cloned by functional complementation of an SOD-deficient Escherichia coli mutant. The nucleotide sequence was determined and the deduced amino acid (aa) sequence (202 aa) showed close similarity to manganese-containing SOD's from other organisms. Subunits of the recombinant L. monocytogenes SOD (re-SOD) and of both E. coli SODs formed enzymatically active hybrid enzymes in vivo. DNA/DNA-hybridization experiments showed that this type of recombinant re-sod gene is conserved within the genus Listeria.}, subject = {Biologie}, language = {en} }