@article{WolfKlugHackenbergetal.1992, author = {Wolf, Markus and Klug, J{\"o}rg and Hackenberg, Reinhard and Gessler, Manfred and Grzeschik, Karl-Heinz and Beato, Miguel and Suske, Guntram}, title = {Human CC10, the homologue of rabbit uteroglobin: genomic cloning, chromosomal localization and expression in endometrial cell lines}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59206}, year = {1992}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{WittbrodtLammersMalitscheketal.1992, author = {Wittbrodt, Joachim and Lammers, Reiner and Malitschek, Barbara and Ullrich, Axel and Schartl, Manfred}, title = {Xmrk receptor tyrosine kinase is activated in Xiphophorus malignant melanoma}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61699}, year = {1992}, abstract = {Xmrk encodes a putative transmembrane glycoprotein of the tyrosine kinase family and is a melanoma-inducing gene in Xiphophorus. We attempted to investigate the biological function of the putative Xmrk receptor by characterizing its signalling properties. Since a potential Iigand for Xmrk has not yet been identified, it has been difficult to analyse the biochemical properlies and biological function of this cell surface protein. In an approach towards such analyses, the Xmrk extracellular domain was replaced by the closely related Iigand-binding domain sequences of the human epidennal growth factor receptor (HER) and the ligand-induced activity of the chimeric HER-Xmrk proteinwas examined. We show that the Xmrk protein is a functional receptor tyrosine kinase, is highly active in malignant melanoma and displays a constitutive autophosphorylation activity possibly due to an activating mutation in its extracellular or transmembrane domain. In the focus formation assay the HER-Xmrk chimera is a potent transfonning protein equivalent to other tyrosine kinase oncoproteins.}, subject = {Physiologische Chemie}, language = {en} } @article{WinklerHongWittbrodtetal.1992, author = {Winkler, Christoph and Hong, Yunhan and Wittbrodt, Joachim and Schartl, Manfred}, title = {Analysis of heterologous and homologous promoters and enhancers in vitro and in vivo by gene transfer into Japanese medaka (Oryzias latipes) and Xiphophorus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86796}, year = {1992}, abstract = {Efficient expression systems are required for analysis of gene regulation and function in teleost fish. To develop such systems, a nurober of inducible or constitutive promoter and enhancer sequences of fish or higher vertebrate origin were tested for activity in a variety of fish celllines andin embryos of the Japanese medaka fish (Oryzias latipes) and Xiphophorus. The activity of the different promoterenhancer combinations were quantitated. Considerable differences were found for some constructs if tested in vitro or in vivo. From the data obtained, a set of expression vectors for basic research as weH as for aquaculture purposes were established.}, subject = {Schwertk{\"a}rpfling}, language = {en} } @article{WeisingFiala1992, author = {Weising, Kurt and Fiala, Brigitte}, title = {Botanische Eindr{\"u}cke vom Bako-Nationalpark / Sarawak}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42947}, year = {1992}, abstract = {No abstract available}, language = {de} } @article{VortkampFranzGessleretal.1992, author = {Vortkamp, Andrea and Franz, Thomas and Gessler, Manfred and Grzeschik, Karl-Heinz}, title = {Deletion of GLI3 supports the homology of the human Greig cephalopolysyndactyly syndrome (GCPS) and the mouse mutant extra toes (Xt)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30166}, year = {1992}, abstract = {No abstract available}, language = {en} } @article{SchluppParzefallEpplenetal.1992, author = {Schlupp, Ingo and Parzefall, Jakob and Epplen, J{\"o}rg T. and Nanda, Indrajit and Schmid, Michael and Schartl, Manfred}, title = {Pseudomale behaviour and spontaneous masculinization in the all-female teleost Poecilia formosa (Teleostei: Poeciliidae)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61688}, year = {1992}, abstract = {Pseudosexual behaviour is a rare phenomenon associated with unisexuality in vertebrates. In the gynogenetic, all-female teleost Poecilia formosa, rare individuals occur that resemble males of closely related gonochoristic species both in behaviour and external morphology. These masculinized gynogens and normal gynogens are members of the same clone, as demonstrated by DNA-fingerprinting. The behaviour of these masculinized gynogens is described and compared to the behaviour of the gonochoristic species Poecilia mexicana, P. latipinna and their hybrid as weil as androgen-treated individuals of P. formosa. No statistically significant difTerences were found between masculinized gynogens and hormonetreated individuals nor between the gonochoristic P. mexicana and P. latipinna males. Differences exist between gonochoristic and unisexual species. Passihle causes and effects of masculinized gynogens are discussed.}, subject = {Physiologische Chemie}, language = {en} } @article{SchartlAdam1992, author = {Schartl, Manfred and Adam, Dieter}, title = {Molecular cloning, structural characterization, and analysis of transcription of the melanoma oncogene of xiphophorus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61989}, year = {1992}, abstract = {No abstract available}, subject = {Physiologische Chemie}, language = {en} } @article{ReinhardHalbrueggeScheeretal.1992, author = {Reinhard, Matthias and Halbr{\"u}gge, Maria and Scheer, Ulrich and Wiegand, Christiane and Jockusch, Brigitte M. and Walter, Ulrich}, title = {The 46/50 kDa phosphoprotein VASP purified from human platelets is a novel protein associated with actin filaments and focal contacts}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34246}, year = {1992}, abstract = {Vasoactive agents which elevate either cGMP or cAMP inhibit platelet activation by pathways sharing at least one component, the 46/50 kDa vasodilator-stimulated phosphoprotein (V ASP). V ASP is stoichiometrically phosphorylated by both cGMP-dependent and cAMPdependent protein kinases in intact human platelets, and its phosphorylation correlates very well with platelet inhibition caused by cGMP- and cAMP-elevating agents. Here we report that in human platelets spread on glass, V ASP is associated predominantly with the distal parts of radial micro filament bundles and with microfilaments outlining the periphery, whereas less V ASP is associated with a central microfilamentous ring. V ASP is also detectable in a variety of different cell types including fibroblasts and epithelial cells. In fibroblasts, V ASP is concentrated at focal contact areas, along microfilament bundles (stress fibres) in a punctate pattern, in the periphery of protruding lamellae, and is phosphorylated by cGMP- and cAMP-dependent protein kinases in response to appropriate stimuli. Evidence for the direct binding of V ASP to F -actin is also presented. The data demonstrate that V ASP is a novel phosphoprotein associated with actin filaments and focal contact areas, i.e. transmembrane junctions between microfilaments and the extracellular matrix.}, language = {de} } @article{OttilieRaulfBarnekowetal.1992, author = {Ottilie, S. and Raulf, F. and Barnekow, A. and Hannig, G. and Schartl, Manfred}, title = {Multiple src-related kinase genes, srk1-4, in the fresh water sponge Spongilla lacustris}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-80282}, year = {1992}, abstract = {In one of the simplest metazoan organisms, the sponge Spongilla lacustris, at least four different src-related kin ase genes (srkl-4) are expressed, aD of which show a high degree of similarity to the c-src genes of vertebrates. Whereas srk2 and srk3 are c1early unrelated at the nucleic acid level, srkl and srk4 share identical sequences in the 5' parts of their cDNAs. The cloning of several primer extension clones and genomic polymerase chain re action experiments confirmed the hypo thesis of an alternative splicing of tandemly arranged carboxyterminal parts of srkl and srk4. The genomic sequence encoding both proteins was found to be interrupted at the splice point by an intron which is located in the same position as one of the introns in the chicken src gene, which is the only gene conserved in invertebrates and vertebrates. All four srk genes are expressed in adult sponges as mRNA transcripts of about 2.2 kb. Tyrosine kin ase activity of a src-related kin ase could be detected in adult sponges but not in their resting form (gemmulae), and may reflect the activity of the srk protein products. Spongilla lacustris is the simplest organism from which a pro tein tyrosine kinase gene has been isolated. The presence of at least four such genes in the evolutionary ancient and primitive phylum Porifera suggests that tyrosine kinase genes arose concomitantly with or shortly after the appearance of multicellular organisms and that their activity may be involved in aggregation and cell-cell recognition.}, subject = {Spongilla lacustris}, language = {en} } @article{NandaSchartlFeichtingeretal.1992, author = {Nanda, Indrajit and Schartl, Manfred and Feichtinger, Wolfgang and Epplen, J{\"o}rg T. and Schmid, Michael}, title = {Early stages of sex chromosome differentiation in fish as analysed by simple repetitive DNA sequences}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61715}, year = {1992}, abstract = {Animal sex chromosome evolution has started on different occasions with a homologous pair of autosomes leading to morphologically differentiated gonosomes. In contrast to other vertebrate classes, among fishes cytologically dernonstrahle sex chromosomes are rare. In reptiles, certain motifs of simple tandemly repeated DNA sequences like (gata)\(_n\)/(gaca)\(_m\) are associated with the constitutive heterochromatin of sex chromosomes. In this study a panel of simple repetitive sequence probes was hybridized to restriction enzyme digested genomic DNA of poeciliid fishes. Apparent male heterogamety previously established by genetic experiments in Poecilia reticulata (guppy) was correlated with male-specific hybridization using the (GACA)\(_4\) probe. The (GATA)\(_4\) oligonucleotide identifies certain male guppies by a Y chromosomal polymorphism in the outbred population. In cantrast none of the genetically defined heterogametic situations in Xiphophorus could be verified consistently using the collection of simple repetitive sequence probes. Only individuals from particular populations produced sex-specific patterns of hybridization with (GATA)\(_4\). Additional poeciliid species (P. sphenops, P. velifera) harbour different sex-specifically organized simple repeat motifs. The observed sex-specific hybridization patterns were substantiated by banding analyses of the karyotypes and by in situ hybridization using the (GACA)\(_4\) probe.}, subject = {Physiologische Chemie}, language = {en} } @article{MuehlenbergHovestadt1992, author = {M{\"u}hlenberg, Michael and Hovestadt, Thomas}, title = {Das Zielartenkonzept}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30140}, year = {1992}, abstract = {No abstract available}, language = {de} } @article{MaschwitzFialaLinsenmair1992, author = {Maschwitz, Ulrich and Fiala, Brigitte and Linsenmair, K. Eduard}, title = {A new ant-tree from SE Asia: Zanthoxylum myriacanthum (Rutaceae), the Thorny Ivy-Rue}, isbn = {0025-1291}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-42967}, year = {1992}, abstract = {Zanthoxylum myriacanthum, a small Rutaceous tree growing mainly in secondary hill forests in SE Asia, is a true myrmecophyte. It possesses stem domatia in the form of hollow branches with slitlike openings. Branch hollows and entrance slits are produced by the plant itself through pith degene~.tion ?u.d growth proceSses. If the entrance is not kept open by ants it closes again by growth ol the surrounding tissue after some time. The domatia are colonized opportunistic ally by different arboreous ants, e.g. Crematogaster and Campono tus. Additionally many small extrafloral nectaries are found on the leaflets of Zanthoxylum myriacanthum. Judging from herbarium studies and literature records at least four more true ant trees are found in the genus Zanthoxylum namely Z. rhetsa in SE Asia, Z. conspersipunctatum, Z. pluviatile and Z. vinkii in New Guinea. We could not confirm ant inhabitation in Drypetes pendula (Euphorbiaceae) on the Malay Peninsula, which has also been recorded to be an anttree.}, language = {en} } @article{KruseTonySebald1992, author = {Kruse, N. and Tony, H. P. and Sebald, Walter}, title = {Conversion of human interleukin-4 into a high affinity antagonist by a single amino acid replacement}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62469}, year = {1992}, abstract = {lnterleukin-4 (IL-4) represents a prototypic lymphokine (for a recent review see Paul, 1991). It promotes differentiation of B-cells and the proliferation of T- and B-cell, and other cell types of the lymphoid system. An antagonist of human IL-4 was discovered during the studies presented here after Tyr124 of the recombinant proteinbad been substituted by an aspartic acid residue. This IL-4 variant, Y124D, bound with high affinity to the IL-4 receptor (K\(_D\) = 310 pM), but retained no detectable proliferative activity for T -<:ells and inhibited IL-4-dependent T -cell proliferation competitively (K\(_i\) = 620 pM). The loss of efficacy in variant Y124D was estimated to be > 100-fold on the basis of a weak partial agonist activity for the very sensitive induction of CD23 positive B-cells. The subsitution of Tyr124 by either phenylalanine, histidine, asparagine, Iysine or glycine resulted in partial agonist variants with unaltered receptor binding atTmity and relatively small deficiencies in efficacy. These results demoostrate that high affinity binding and signal generation can be uncoupled efticiently in a Iigand of a receptor betonging to the recently identified hematopoietin receptor family. In addition we show for the first time, that a powerful antagonist acting on the IL-4 receptor system can be derived from the IL-4 protein.}, subject = {Biochemie}, language = {en} } @article{KobeltLinsenmair1992, author = {Kobelt, F. and Linsenmair, Karl Eduard}, title = {Adaptations of the reed frog Hyperolius viridiflavus (Hyperoliidae) to its arid environment. VI. The iridophores in the skin as radiation reflectors}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-30563}, year = {1992}, abstract = {No abstract available}, language = {en} } @article{HongSchartl1992, author = {Hong, Yunhan and Schartl, Manfred}, title = {Structure of the rainbow trout metallothionein A gene}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61679}, year = {1992}, abstract = {To investigate the regulation of metallothionein-encoding genes in fish, we have isolated and sequenced the rainbow trout metallothionein-A-encoding gene (tMT-A) by polymerase chain reaction. This gene spans about 1.1 kb, consists of three exons and two introns, and has an A+ T-rieb 5' -region which contains a TATAAA signal, and two metal responsive elements (MREs). The transcription start point is centered around an A residue 81 nt upstream of the ATG codon.}, subject = {Physiologische Chemie}, language = {en} } @article{HaasDumbskyKreft1992, author = {Haas, Albert and Dumbsky, Martina and Kreft, J{\"u}rgen}, title = {Listeriolysin genes: complete sequence of ilo from Listeria ivanovii and of lso from Listeria seeligeri}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60529}, year = {1992}, abstract = {The completc DNA scqucnccs coding for thc thiol-activated cytolysins from Listeria ivanovii, ivanolysin 0 (ILO) and for sccligerolysin 0 (LSO) from Listeria seeligeri have been dctermined. Thc deduced amino acid scquences revealed that: (i) the primary translation products comprise 528 (ILO) and 530 (LSO) amino acids. respectively. (ii) ILO contains two cysteines. LSO has a substitution in the conserved cysteine motif.}, subject = {Biologie}, language = {en} } @article{GoetzRaulfSchartl1992, author = {G{\"o}tz, Rudolf and Raulf, Friedrich and Schartl, Manfrad}, title = {Brain-derived neurotrophic factor is more highly conserved in structure and function than nerve growth factor during vertebrate evolution}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-61703}, year = {1992}, abstract = {Mammalian nerve growth factor (NGF) and brain-derived neurotrophic factor (BDNF) are members of a protein family with perfectly conserved domains arranged around the cysteine residues thought to stabilize an invariant three-dimensional scaffold in addition to distinct sequence motifs that convey different neuronal functions. To study their structural and functional conservation during evolution, we have compared NGF and BDNF from a lower vertebrate, the teleost fi.sh Xiphophorus, with the mammalian homlogues. Genomic clones encoding fish NGF and BDNF were isolated by cross-hybridization using probes from the cloned mammalian factors. Fish NGF and BDNF were expressed by means of recombinant vaccinia viruses, purified, and their neuronal survival specificities for different classes of neurons were found to mirror those of the mammalian factors. The half-maximal survival concentration for chick sensory neurons was 60 pg/ml for both fish and mammalian purifi.ed recombinant BDNF. However, the activity ofrecombinant fish NGF on both chick sensory and sympathetic neurons was 6 ng,lml, 75-fold lower than that of mouse NGF. The different functional conservation of NGF and BDNF is also reflected in their structures. The DNA-deduced amino acid sequences of processed mature fish NGF and BDNF showed, compared to mouse, 63\% and 90\% identity, respectively, indicating that NGF bad reached an optimized structure later than BDNF. The retrograde extrapolation of these data indicates that NGF and BDNF evolved at strikingly different rates ftom a common ancestral gene about 600 million years ago. By RNA gel blot anaJysis NGF mRNA was detected during late embryonie development; BDNF was present in adult brain.}, subject = {Physiologische Chemie}, language = {en} } @article{GrafeSchmuckLinsenmair1992, author = {Grafe, T. Ulmar and Schmuck, Richard and Linsenmair, Karl Eduard}, title = {Reproductive energetics of the African Reed Frogs, Hyperolius viridiflavus and Hyperolius marmoratus}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-31187}, year = {1992}, abstract = {No abstract available}, language = {en} } @article{GesslerKoenigBruns1992, author = {Gessler, Manfred and K{\"o}nig, A. and Bruns, G. A. P.}, title = {The genomic organization and expression of the WT1 gene}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59195}, year = {1992}, abstract = {The Wilms tumor gene WTl, a proposed tumor suppressor gene, has been identifled based on its location within a homozygous deletion found in tumor tissue. The gene encodes a putative transcription factor containing a Cys/His zinc finger domain. The critical homozygous deletions, however, are rarely seen, suggesting that in many cases the gene may be inactivated by more subtle alterations. To facilitate the seareh for smaller deletions and point mutations we have established the genomic organization of the WTl gene and have determined the sequence of all 10 exons and flanking intron DNA. The pattern of alternative splicing in two regions has been characterized in detail. These results will form the basis for future studies of mutant alleles at this locus.}, subject = {Biochemie}, language = {en} } @article{GesslerGrupeGrzeschiketal.1992, author = {Gessler, Manfred and Grupe, Andrew and Grzeschik, Karl-Heinz and Pongs, Olaf}, title = {The potassium channel gene HK1 maps to human chromosome 11p14.1, close to the FSHB gene}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-59184}, year = {1992}, abstract = {Transiently activating (A-type) potassium (K) channels are important regulators of action potential and action potential firing frequencies. HK1 designates the firsthuman cDNA that is highly homologous to the rat RCK4 cDNA that codes for an A-type K-channel. The HK1 channel is expressed in heart. By somatic cell hybrid analysis, the HK1 gene has been assigned to human chromosome 11p13-pl4, the WAGR deletion region (Wilms tumor, aniridia, genito-urinary abnormalities and mental retardation). Subsequent pulsed field gel (PFG) analysis and comparison with the well-established PFG map of this region localized the gene to 11p14, 200-600 kb telomeric to the FSHB gene.}, subject = {Biochemie}, language = {en} } @article{FischerWeisenbergerScheer1992, author = {Fischer, D. and Weisenberger, D. and Scheer, Ulrich}, title = {In situ hybridization of DIG-labeled rRNA probes to mouse liver ultrathin sections}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-69458}, year = {1992}, abstract = {No abstract available.}, subject = {Hybridisierung }, language = {en} } @article{FialaMaschwitz1992, author = {Fiala, Brigitte and Maschwitz, Ulrich}, title = {Food bodies and their significance for obligate ant-association in the tree genus Macaranga (Euphorbiaceae)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32921}, year = {1992}, abstract = {The production of extrafloral nectar and food bodies plays an important role in many tropical ant-plant mutualisms. In Malaysia, a close association exists between ants and some species of the pioneer tree genus Macaranga (Euphorbiaccac). Macaranga is a very diverse genus which exhibits all stages ofintcraction with ants, from facultative to obligatory associations. The ants nest inside the hollow inlcrnodes and reed mainly on food budies provided by the plants. Food body production had previously been reported only in myrrnecophytic Macaranga species, where it is usually coneentrated on protected parts or the plants such as recurved stipules. We found that non-myrmecophytic Macaranga species also produce food bodies on leaves and stems, where they are collected by a variety or ants. Levels of food body production differ between facultatively and obligatorily ant-associated species but also among the various non-myrmecophytes. This may he rdated to the degree of interaction with ants. Food body production starts at a younger age in the myrmccophytic species than in the transitional or non-myrmcccophytic Macaranga. Although food bodies of the non-inhabited Macaranga species are collected by a variety of ants, there is nu evidence of association with specific ant species. Our observations suggest that food bodies enhance the evolution of ant-plant interactions. Production of food bodies alone, however, does not appear to be the most important factor for the development of obligate myrmccopllytism in Macaranga.}, language = {en} } @article{FialaMaschwitz1992, author = {Fiala, Brigitte and Maschwitz, Ulrich}, title = {Domatia as most important adaptations in the evolution of myrmecophytes in the paleotropical tree genus Macaranga (Euphorbiacae)}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-32935}, year = {1992}, abstract = {The paleotropical tree genus Macaranga (Euphorbiaceae) comprises all stages of interaction with ants, from facultative associations to obligate myrmecophytes. In SE.-Asia food availability does not seem to be the limiting factor for the development of a close relationship since all species provide food for ants in form of extrafloral nectar and/or food bodies. Only myrmecophytic Macaranga species offer nesting space for ants (domatia) inside intern odes which become hollow due to degeneration of the pith. Non-myrmecophytic species have a solid stem with a compact and wet pith and many resin ducts. The stem interior of some transitional species remains solid, but the soft pith can be excavated. The role of different ant-attracting attributes for the development of obligate ant-plant interactions is discussed. In the genus Macaranga, the provision of nesting space seems to be the most important factor for the evolution of obligate myrmecophytism.}, language = {en} } @article{DuschlJahnBertlingetal.1992, author = {Duschl, Albert and Jahn, Ute and Bertling, Claudia and Sebald, Walter}, title = {A comparison of assays for the response of primary human T-cells upon stimulation with interleukin-2, interleukin-4 and interleukin-7}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-86750}, year = {1992}, abstract = {The most commonly used assay to quantitate the response of peripheral T~cells upon stimulation with growth factors is determination of incorporated (JH]TdR. We compared thls test to three other methods: 1. direct countlog of cells with a Coulter type counter as reference assay, 2. a colorimetric assay using the tetrazolium dye 3-[ 4,S-dimethylthiazol-l-yl]-2,5diphenyl tetrazolium (MTT), which is a cheap and increasingly popular non-radioactive method and 3. incorporation of the thymidine analog 5-bromo-2'-deoxyuridine detection with a monoclonal antibody on cytospins. Primary human PHA-blasts from >30 healthy individuals were stimulated with IL-2, IL-4 aod IL-7 and assayed with up to four different methods. We discuss the advantages and disadvantages of the assays used and tbe effects of differences between cell preparations. We observed no significant variations between individuals for the dose dependence, but the relative emctency of IL4 compared to IL-2 and IL-7 was variable. This was probably due to the slower response observed upon stimulation with this factor.}, subject = {T-Lymphozyt}, language = {en} } @article{DummerPosseckertNestleetal.1992, author = {Dummer, R. and Posseckert, G. and Nestle, F. and Witzgall, R. and Burger, M. and Becker, J. C. and Sch{\"a}fer, E. and Wiede, J. and Sebald, Walter and Burg, G.}, title = {Soluble interleukin-2 receptors inhibit interleukin 2-dependent proliferation and cytotoxicity: explanation for diminished natural killer cell activity in cutaneous T-cell lymphomas in vivo?}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-62473}, year = {1992}, abstract = {No abstract available}, subject = {Biochemie}, language = {en} } @article{DandekarTollervey1992, author = {Dandekar, Thomas and Tollervey, David}, title = {Mutational analysis of Schizosaccharomyces pombe U4 snRNA by plasmid exchange}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29969}, year = {1992}, abstract = {No abstract available}, language = {en} } @misc{DandekarArgos1992, author = {Dandekar, Thomas and Argos, Patrick}, title = {A novel heterodimeric cysteine protease is required for interleukin 1ß processing in monocytes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29986}, year = {1992}, abstract = {No abstract available}, language = {en} } @misc{DandekarArgos1992, author = {Dandekar, Thomas and Argos, Patrick}, title = {Successive action of DnaK, DnaJ and GroEL along the pathway of chaperone-mediated protein folding}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29814}, year = {1992}, abstract = {No abstract available}, language = {en} } @article{DandekarArgos1992, author = {Dandekar, Thomas and Argos, P.}, title = {Potential of genetic algorithms in protein folding and protein engineering simulations}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-29974}, year = {1992}, abstract = {No abstract available}, language = {de} } @article{BrehmHaasGoebeletal.1992, author = {Brehm, Klaus and Haas, Albert and Goebel, Werner and Kreft, J{\"u}rgen}, title = {A gene encoding a superoxide dismutase of the facultative intracellular bacterium Listeria monocytogenes}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-60515}, year = {1992}, abstract = {A gene (Imsod) encoding superoxide dismutase (SOD; EC 1.15.1.1) of the facultative intracellular pathogen, Listeria monocytogenes, was cloned by functional complementation of an SOD-deficient Escherichia coli mutant. The nucleotide sequence was determined and the deduced amino acid (aa) sequence (202 aa) showed close similarity to manganese-containing SOD's from other organisms. Subunits of the recombinant L. monocytogenes SOD (re-SOD) and of both E. coli SODs formed enzymatically active hybrid enzymes in vivo. DNA/DNA-hybridization experiments showed that this type of recombinant re-sod gene is conserved within the genus Listeria.}, subject = {Biologie}, language = {en} } @article{BellDabauvalleScheer1992, author = {Bell, Peter and Dabauvalle, Marie-Christine and Scheer, Ulrich}, title = {In vitro assembly of prenucleolar bodies in Xenopus egg extract}, url = {http://nbn-resolving.de/urn:nbn:de:bvb:20-opus-34233}, year = {1992}, abstract = {Nuclei assembled in Xenopus egg extract from purified DNA or chromatin resemble their natural counterparts in a number of structural and functional features. However, the most obvious structural element of normal interphase nuclei, the nucleolus, is absent from the in vitro reconstituted nuclei. By EM, cytological silver staining, and immunofluorescence microscopy employing antibodies directed against various nucleolar components we show that nuclei assembled in vitro contain numerous distinct aggregates that resemble prenucleolar bodies (PNBs) by several criteria. Formation of these PNB-like structures requires pore complex-mediated nuclear transport of proteins but is independent of the genetic content of the in vitro nuclei as well as transcriptional and translational events. Our data indicate that nuclei assembled in vitro are capable of initiating early steps of nucleologenesis but that the resulting PNBs are unable to fuse with each other, probably due to the absence of a functional nucleolus organizer. With appropriate modifications, this experimental system should be useful to define and analyze conditions promoting the site-specific assembly of PNBs into a coherent nucleolar body.}, language = {en} }